Restriction map for
pFN21AB6253
(Circular) (Six-base) MAPSORT of: pFN21AB6253.seq Check: 8387 from: 1 to: 6625
pFN21AB6253 6625 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2932 2932
Size: 6625
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4435 278
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3968 1937
Size: 2031 4594
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 2344 4752 5125 1563
Size: 781 2408 373 3063
Fragments arranged by size:
3063 2408 781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 2775 3120 3476 3593 4688 6072 6340
Size: 545 598 345 356 117 1095 1384 268
Cuts at: 6340 1632
Size: 1917
Fragments arranged by size:
1917 1384 1095 598 545 356 345 268
117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 2800 5524 6501 1203
Size: 1597 2724 977 1327
Fragments arranged by size:
2724 1597 1327 977
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6625
AlwNI CAG_nnn'CTG
Cuts at: 3825 4368 5940 3825
Size: 543 1572 4510
Fragments arranged by size:
4510 1572 543
ApaLI G'TGCA_C
Cuts at: 3030 4682 5838 3030
Size: 1652 1156 3817
Fragments arranged by size:
3817 1652 1156
ApoI r'AATT_y
Cuts at: 1493 2560 3176 3934 4160 4312 6421 1493
Size: 1067 616 758 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 758 616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 2978 3950 1870
Size: 73 193 842 972 4545
Fragments arranged by size:
4545 972 842 193 73
BamHI G'GATC_C
Cuts at: 3082 3955 4322 3082
Size: 873 367 5385
Fragments arranged by size:
5385 873 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3143 3730 3946 5401 618
Size: 325 217 627 1356 587 216 1455 1842
Fragments arranged by size:
1842 1455 1356 627 587 325 217 216
BanII G_rGCy'C
Cuts at: 1391 2274 3803 3944 1391
Size: 883 1529 141 4072
Fragments arranged by size:
4072 1529 883 141
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2020 2463 2601 2871 3756 928
Size: 971 121 443 138 270 885 3797
Fragments arranged by size:
3797 971 885 443 270 138 121
BbvCI CC'TCA_GC
Cuts at: 2823 2823
Size: 6625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 4816 4850 1903
Size: 34 1850 34 995 34 3678
Fragments arranged by size:
3678 1850 995 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4517 5727 4517
Size: 1210 5415
BclI T'GATC_A
Cuts at: 1548 3507 1548
Size: 1959 4666
BfrBI ATG'CAT
Cuts at: 3899 3899
Size: 6625
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5236 136
Size: 107 122 71 631 605 3564 1525
Fragments arranged by size:
3564 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3493 6620 3493
Size: 3127 3498
BlpI GC'TnA_GC
Cuts at: 4042 4042
Size: 6625
Bme1580I G_kGCm'C
Cuts at: 1790 3034 3146 4686 5842 1790
Size: 1244 112 1540 1156 2573
Fragments arranged by size:
2573 1540 1244 1156 112
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6625
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2498 3649 4356
Size: 416 53 83 399 24 1081 1151 707
Cuts at: 4356 5314 442
Size: 958 1753
Fragments arranged by size:
1753 1151 1081 958 707 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2755 5285 1239
Size: 1516 2530 2579
Fragments arranged by size:
2579 2530 1516
Bpu10I CC'TnA_GC
Cuts at: 2268 2676 2823 2268
Size: 408 147 6070
Fragments arranged by size:
6070 408 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3417 4750 5615 5913 3417
Size: 1333 865 298 4129
Fragments arranged by size:
4129 1333 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3309 5288 882
Size: 617 1810 1979 2219
Fragments arranged by size:
2219 1979 1810 617
BsaAI yAC'GTr
Cuts at: 493 1204 3892 4416 493
Size: 711 2688 524 2702
Fragments arranged by size:
2702 2688 711 524
BsaBI GATnn'nnATC
Cuts at: 6619 6619
Size: 6625
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4432 4814 5449 275
Size: 53 83 186 936 2899 382 635 1451
Fragments arranged by size:
2899 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5058 5456 5730 5877 6288 5058
Size: 398 274 147 411 5395
Fragments arranged by size:
5395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6400
Fragments arranged by size:
6400 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3869 1186
Size: 2683 3942
BseYI C'CCAG_C
Cuts at: 3361 5828 6324 3361
Size: 2467 496 3662
Fragments arranged by size:
3662 2467 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 1163
Size: 573 516 5536
Fragments arranged by size:
5536 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 3034 4686 4771 5842 2274
Size: 760 1652 85 1071 3057
Fragments arranged by size:
3057 1652 1071 760 85
BsmI GAATG_Cn'
Cuts at: 3899 3901 4136 4229 3899
Size: 2 235 93 6295
Fragments arranged by size:
6295 235 93 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 3034 3146 3803 3944 4686 4771
Size: 399 484 760 112 657 141 742 85
Cuts at: 4771 5842 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 760 742 657 484 399 141
112 85
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 4514 6244 4514
Size: 1730 4895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2932 3841 3980 844
Size: 2088 909 139 3489
Fragments arranged by size:
3489 2088 909 139
BsrBI CCG'CTC
Cuts at: 1394 1823 4512 1394
Size: 429 2689 3507
Fragments arranged by size:
3507 2689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2997 5120 5294 65
Size: 1183 1749 2123 174 1396
Fragments arranged by size:
2123 1749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5269 5456 1700
Size: 110 3459 187 2869
Fragments arranged by size:
3459 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2323 96
Size: 2227 4398
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 4685 5697 4685
Size: 1012 5613
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6625
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 3082 3493 3955 4322 4707 4724 6176 6262
Size: 1110 411 462 367 385 17 1452 86
Cuts at: 6262 6274 6620 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1110 462 411 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 2240 2637 4141 4935 4955 2044
Size: 196 397 1504 794 20 3714
Fragments arranged by size:
3714 1504 794 397 196 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 3929 3929
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5632 809
Size: 507 4316 1802
Fragments arranged by size:
4316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2634 2710 4072
Size: 54 1425 50 275 18 804 76 1362
Cuts at: 4072 4961 6590 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1362 889 804 275 76 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2445 4555 1353
Size: 783 309 2110 3423
Fragments arranged by size:
3423 2110 783 309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2673 5212 5586 5732 387
Size: 1064 138 1084 2539 374 146 1280
Fragments arranged by size:
2539 1280 1084 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2272 2272
Size: 6625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2755 2775 3120 3476 3593 4688
Size: 393 545 578 20 345 356 117 1095
Cuts at: 4688 5285 6072 6340 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1095 787 597 578 545 393 356
345 268 117 20
EcoO109I rG'GnC_Cy
Cuts at: 2282 3789 2282
Size: 1507 5118
EcoRI G'AATT_C
Cuts at: 2560 3934 4312 2560
Size: 1374 378 4873
Fragments arranged by size:
4873 1374 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 3187 2131
Size: 1056 5569
FspI TGC'GCA
Cuts at: 5131 5131
Size: 6625
HaeII r_GCGC'y
Cuts at: 1678 3111 5772 1678
Size: 1433 2661 2531
Fragments arranged by size:
2661 2531 1433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3074 3106 3451 3463 3483
Size: 32 135 32 1502 32 345 12 20
Cuts at: 3483 3495 3581 3613 5266 5298 5340 5372 1373
Size: 12 86 32 1653 32 42 32 2626
Fragments arranged by size:
2626 1653 1502 345 135 86 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2725 2916 3969 4215 669
Size: 988 281 787 191 1053 246 3079
Fragments arranged by size:
3079 1053 988 787 281 246 191
HindIII A'AGCT_T
Cuts at: 748 2641 748
Size: 1893 4732
HpaI GTT'AAC
Cuts at: 2725 4215 2725
Size: 1490 5135
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2555 3626 5739 5923 1305
Size: 1250 1071 2113 184 2007
Fragments arranged by size:
2113 2007 1250 1071 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2636 2712 10
Size: 54 1425 343 804 76 3923
Fragments arranged by size:
3923 1425 804 343 76 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2856 4583 4942 5101 518
Size: 648 913 777 1727 359 159 2042
Fragments arranged by size:
2042 1727 913 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2831 3080 4041 4718 5866 6111 1606
Size: 1225 249 961 677 1148 245 2120
Fragments arranged by size:
2120 1225 1148 961 677 249 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6625
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6625
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NsiI A_TGCA'T
Cuts at: 3901 3901
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2804 2970 3422 3983 5528 1132
Size: 97 1575 166 452 561 1545 2229
Fragments arranged by size:
2229 1575 1545 561 452 166 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 2800 5524 2800
Size: 2724 3901
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6625
PfoI T'CCnGG_A
Cuts at: 2441 2441
Size: 6625
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2282 3789 2282
Size: 1507 5118
PshAI GACnn'nnGTC
Cuts at: 1613 3917 1613
Size: 2304 4321
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PstI C_TGCA'G
Cuts at: 830 2604 3501 3977 830
Size: 1774 897 476 3478
Fragments arranged by size:
3478 1774 897 476
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 2831 2831
Size: 6625
RsrII CG'GwC_CG
Cuts at: 2336 2336
Size: 6625
SacI G_AGCT'C
Cuts at: 2274 2274
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 2259 4873 6585 2259
Size: 2614 1712 2299
Fragments arranged by size:
2614 2299 1712
SfcI C'TryA_G
Cuts at: 826 1045 2600 2688 2790 3497 3973 5108 5789
Size: 219 1555 88 102 707 476 1135 681
Cuts at: 5789 5980 826
Size: 191 1471
Fragments arranged by size:
1555 1471 1135 707 681 476 219 191
102 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 2138 2980 3952 1872
Size: 266 842 972 4545
Fragments arranged by size:
4545 972 842 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3432 4729 5630 5892 820
Size: 197 926 1489 1297 901 262 1553
Fragments arranged by size:
1553 1489 1297 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3983 1132
Size: 2851 3774
SspI AAT'ATT
Cuts at: 5 52 3706 4549 5
Size: 47 3654 843 2081
Fragments arranged by size:
3654 2081 843 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6625
StyI C'CwwG_G
Cuts at: 513 1065 2004 2285 4064 513
Size: 552 939 281 1779 3074
Fragments arranged by size:
3074 1779 939 552 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4652 4669 4822 5007 5452 325
Size: 1255 3072 17 153 185 445 1498
Fragments arranged by size:
3072 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2257 2323 4871
Size: 274 80 33 51 1443 280 66 2548
Cuts at: 4871 6583 96
Size: 1712 138
Fragments arranged by size:
2548 1712 1443 280 274 138 80 66
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2928 2960 6585 6617 2928
Size: 32 3625 32 2936
Fragments arranged by size:
3625 2936 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6625
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2081 2081
Size: 6625
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 2136 2978 3950 1870
Size: 266 842 972 4545
Fragments arranged by size:
4545 972 842 266
XmnI GAAnn'nnTTC
Cuts at: 3899 4754 3899
Size: 855 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4433 276
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI