Restriction map for
pFN21AB6251
(Circular) (Six-base) MAPSORT of: pFN21AB6251.seq Check: 2258 from: 1 to: 6985
pFN21AB6251 6985 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4795 278
Size: 53 83 186 936 3259 2468
Fragments arranged by size:
3259 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4328 1937
Size: 2391 4594
Acc65I G'GTAC_C
Cuts at: 2945 4306 2945
Size: 1361 5624
AclI AA'CG_TT
Cuts at: 1563 5112 5485 1563
Size: 3549 373 3063
Fragments arranged by size:
3549 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2712 3402 5048 6432 6700 1632
Size: 1080 690 1646 1384 268 1917
Fragments arranged by size:
1917 1646 1384 1080 690 268
AflII C'TTAA_G
Cuts at: 820 1017 2519 820
Size: 197 1502 5286
Fragments arranged by size:
5286 1502 197
AflIII A'CryG_T
Cuts at: 1203 5884 6861 1203
Size: 4681 977 1327
Fragments arranged by size:
4681 1327 977
AgeI A'CCGG_T
Cuts at: 5816 5816
Size: 6985
AhdI GACnn_n'nnGTC
Cuts at: 3585 5714 3585
Size: 2129 4856
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6985
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6985
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3270 3302 3182
Size: 32 56 32 6865
Fragments arranged by size:
6865 56 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3281 3611 4728 6300 3281
Size: 330 1117 1572 3966
Fragments arranged by size:
3966 1572 1117 330
ApaLI G'TGCA_C
Cuts at: 5042 6198 5042
Size: 1156 5829
ApoI r'AATT_y
Cuts at: 1493 3849 3905 4294 4520 4672 6781 1493
Size: 2356 56 389 226 152 2109 1697
Fragments arranged by size:
2356 2109 1697 389 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 5539 160
Size: 872 4507 1606
Fragments arranged by size:
4507 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
AvaI C'yCGr_G
Cuts at: 1870 1943 4310 1870
Size: 73 2367 4545
Fragments arranged by size:
4545 2367 73
BamHI G'GATC_C
Cuts at: 4315 4682 4315
Size: 367 6618
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2793 2945 3530 4306 5761
Size: 325 217 627 1006 152 585 776 1455
Cuts at: 5761 618
Size: 1842
Fragments arranged by size:
1842 1455 1006 776 627 585 325 217
152
BanII G_rGCy'C
Cuts at: 1391 2718 4304 1391
Size: 1327 1586 4072
Fragments arranged by size:
4072 1586 1327
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6014
BbvCI CC'TCA_GC
Cuts at: 3108 3108
Size: 6985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5176 5210 1903
Size: 34 3239 34 3678
Fragments arranged by size:
3678 3239 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2803 3910 4877 6087 2803
Size: 1107 967 1210 3701
Fragments arranged by size:
3701 1210 1107 967
BclI T'GATC_A
Cuts at: 1548 2185 2248 4066 1548
Size: 637 63 1818 4467
Fragments arranged by size:
4467 1818 637 63
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5596 136
Size: 107 122 71 631 605 3924 1525
Fragments arranged by size:
3924 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6980 6980
Size: 6985
BlpI GC'TnA_GC
Cuts at: 4402 4402
Size: 6985
Bme1580I G_kGCm'C
Cuts at: 1790 5046 6202 1790
Size: 3256 1156 2573
Fragments arranged by size:
3256 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6985
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2749 3103 4716
Size: 416 53 83 399 24 1332 354 1613
Cuts at: 4716 5674 442
Size: 958 1753
Fragments arranged by size:
1753 1613 1332 958 416 399 354 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3303 3510 3798 5645 1239
Size: 2064 207 288 1847 2579
Fragments arranged by size:
2579 2064 1847 288 207
Bpu10I CC'TnA_GC
Cuts at: 3108 3807 3108
Size: 699 6286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2693 3538 5110 5975 6273 2693
Size: 845 1572 865 298 3405
Fragments arranged by size:
3405 1572 865 845 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5648 882
Size: 617 4149 2219
Fragments arranged by size:
4149 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4776 493
Size: 711 3572 2702
Fragments arranged by size:
3572 2702 711
BsaBI GATnn'nnATC
Cuts at: 6979 6979
Size: 6985
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3819 4792 5174 5809
Size: 53 83 186 936 2286 973 382 635
Cuts at: 5809 275
Size: 1451
Fragments arranged by size:
2286 1451 973 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2718 5418 5816 6090 6237 6648 2718
Size: 2700 398 274 147 411 3055
Fragments arranged by size:
3055 2700 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3508 3538 1178
Size: 30 165 30 2105 30 4625
Fragments arranged by size:
4625 2105 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6985
BseYI C'CCAG_C
Cuts at: 6188 6684 6188
Size: 496 6489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3335 3713 1163
Size: 573 1599 378 4435
Fragments arranged by size:
4435 1599 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4435 5196 6224 1540
Size: 275 186 2434 761 1028 2301
Fragments arranged by size:
2434 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2718 5046 5131 6202 2012
Size: 706 2328 85 1071 2795
Fragments arranged by size:
2795 2328 1071 706 85
BsmI GAATG_Cn'
Cuts at: 4496 4589 4496
Size: 93 6892
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2718 4304 5046 5131 6202 1391
Size: 399 222 706 1586 742 85 1071 2174
Fragments arranged by size:
2174 1586 1071 742 706 399 222 85
BspEI T'CCGG_A
Cuts at: 2718 6648 2718
Size: 3930 3055
BspHI T'CATG_A
Cuts at: 2869 4874 6604 2869
Size: 2005 1730 3250
Fragments arranged by size:
3250 2005 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4340 844
Size: 3496 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2952 4872 1394
Size: 429 1129 1920 3507
Fragments arranged by size:
3507 1920 1129 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2740 2969 3383 5480 5654 65
Size: 1183 1492 229 414 2097 174 1396
Fragments arranged by size:
2097 1492 1396 1183 414 229 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2616 5629 5816 1700
Size: 110 806 3013 187 2869
Fragments arranged by size:
3013 2869 806 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6985
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6985
BssSI C'ACGA_G
Cuts at: 5045 6057 5045
Size: 1012 5973
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6985
BstBI TT'CG_AA
Cuts at: 5789 5789
Size: 6985
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6985
BstYI r'GATC_y
Cuts at: 1972 3097 3278 4177 4315 4682 5067 5084 6536
Size: 1125 181 899 138 367 385 17 1452
Cuts at: 6536 6622 6634 6980 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1125 899 385 367 346 181
138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3050 3050
Size: 6985
BtgI C'CryG_G
Cuts at: 513 1065 3375 513
Size: 552 2310 4123
Fragments arranged by size:
4123 2310 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6246
Fragments arranged by size:
6246 683 56
BtsI GCAGTG_nn'
Cuts at: 2098 3701 4501 5295 5315 2098
Size: 1603 800 794 20 3768
Fragments arranged by size:
3768 1603 800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6985
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6950
DraI TTT'AAA
Cuts at: 4289 4289
Size: 6985
DraIII CAC_nnn'GTG
Cuts at: 2912 2912
Size: 6985
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5992 809
Size: 507 4676 1802
Fragments arranged by size:
4676 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4432 5321 6950
Size: 54 1425 50 275 18 2602 889 1629
Cuts at: 6950 8
Size: 43
Fragments arranged by size:
2602 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4432 1537
Size: 275 2620 4090
Fragments arranged by size:
4090 2620 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 2635 3370 4915 1353
Size: 660 622 735 1545 3423
Fragments arranged by size:
3423 1545 735 660 622
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5572 5946 6092 387
Size: 1064 138 3983 374 146 1280
Fragments arranged by size:
3983 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2716 2716
Size: 6985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2712 3303 3402 3510 3798 5048 5645
Size: 393 1080 591 99 108 288 1250 597
Cuts at: 5645 6432 6700 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1250 1080 787 597 591 393 288
268 108 99
EcoRI G'AATT_C
Cuts at: 4294 4672 4294
Size: 378 6607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2793 2793
Size: 6985
FspI TGC'GCA
Cuts at: 5491 5491
Size: 6985
HaeII r_GCGC'y
Cuts at: 1678 2762 6132 1678
Size: 1084 3370 2531
Fragments arranged by size:
3370 2531 1084
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2053 2085 2345 2377 3257
Size: 32 135 32 481 32 260 32 880
Cuts at: 3257 3289 3571 3603 3776 3808 4133 4165 5626
Size: 32 282 32 173 32 325 32 1461
Cuts at: 5626 5658 5700 5732 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1461 880 481 325 282 260 173
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3442 4329 4575 669
Size: 988 281 939 565 887 246 3079
Fragments arranged by size:
3079 988 939 887 565 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6985
HpaI GTT'AAC
Cuts at: 4575 4575
Size: 6985
KpnI G_GTAC'C
Cuts at: 2949 4310 2949
Size: 1361 5624
MfeI C'AATT_G
Cuts at: 4074 4584 4074
Size: 510 6475
MluI A'CGCG_T
Cuts at: 6861 6861
Size: 6985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4213 6099 6283 1305
Size: 2908 1886 184 2007
Fragments arranged by size:
2908 2007 1886 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5163
Fragments arranged by size:
5163 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2733 3136 3189 4943 5302 5461 518
Size: 648 1567 403 53 1754 359 159 2042
Fragments arranged by size:
2042 1754 1567 648 403 359 159 53
MspA1I CmG'CkG
Cuts at: 1606 2927 3608 4401 5078 6226 6471 1606
Size: 1321 681 793 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 793 681 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3375 513
Size: 552 2310 4123
Fragments arranged by size:
4123 2310 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6985
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6985
NotI GC'GGCC_GC
Cuts at: 4432 4432
Size: 6985
NspI r_CATG'y
Cuts at: 1132 1229 2656 3323 4343 5888 1132
Size: 97 1427 667 1020 1545 2229
Fragments arranged by size:
2229 1545 1427 1020 667 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6985
PciI A'CATG_T
Cuts at: 5884 5884
Size: 6985
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6985
PmeI GTTT'AAAC
Cuts at: 4289 4289
Size: 6985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5054 5086 6567 6599 5054
Size: 32 1481 32 5440
Fragments arranged by size:
5440 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6985
PsiI TTA'TAA
Cuts at: 4555 4555
Size: 6985
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3112 3144 3112
Size: 32 6953
PstI C_TGCA'G
Cuts at: 830 4337 830
Size: 3507 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6985
PvuII CAG'CTG
Cuts at: 2927 3608 2927
Size: 681 6304
SacI G_AGCT'C
Cuts at: 2718 2718
Size: 6985
SalI G'TCGA_C
Cuts at: 1936 4327 1936
Size: 2391 4594
SbfI CC_TGCA'GG
Cuts at: 4337 4337
Size: 6985
ScaI AGT'ACT
Cuts at: 5233 6945 5233
Size: 1712 5273
SfcI C'TryA_G
Cuts at: 826 1045 2544 2590 2598 3476 3509 4050 4333
Size: 219 1499 46 8 878 33 541 283
Cuts at: 4333 5468 6149 6340 826
Size: 1135 681 191 1471
Fragments arranged by size:
1499 1471 1135 878 681 541 283 219
191 46 33 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6985
SmaI CCC'GGG
Cuts at: 1872 4312 1872
Size: 2440 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 2708 3553 5089 5990 6252
Size: 197 926 576 189 845 1536 901 262
Cuts at: 6252 820
Size: 1553
Fragments arranged by size:
1553 1536 926 901 845 576 262 197
189
SnaBI TAC'GTA
Cuts at: 493 4776 493
Size: 4283 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6985
SphI G_CATG'C
Cuts at: 1132 4343 1132
Size: 3211 3774
SspI AAT'ATT
Cuts at: 5 52 2506 4909 5
Size: 47 2454 2403 2081
Fragments arranged by size:
2454 2403 2081 47
StuI AGG'CCT
Cuts at: 1517 3564 1517
Size: 2047 4938
StyI C'CwwG_G
Cuts at: 513 1065 2367 2475 3024 3375 3559 4227 4424
Size: 552 1302 108 549 351 184 668 197
Cuts at: 4424 513
Size: 3074
Fragments arranged by size:
3074 1302 668 552 549 351 197 184
108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3422 5012 5029 5182 5367 5812 325
Size: 1255 1842 1590 17 153 185 445 1498
Fragments arranged by size:
1842 1590 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5231 6943 96
Size: 274 80 33 51 1443 3254 1712 138
Fragments arranged by size:
3254 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2989 3021 3508 3540 6945 6977 2989
Size: 32 487 32 3405 32 2997
Fragments arranged by size:
3405 2997 487 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2900 1316
Size: 1584 5401
XbaI T'CTAG_A
Cuts at: 4321 4321
Size: 6985
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6985
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6985
XmaI C'CCGG_G
Cuts at: 1870 4310 1870
Size: 2440 4545
XmnI GAAnn'nnTTC
Cuts at: 2865 5114 2865
Size: 2249 4736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4793 276
Size: 53 83 186 936 3259 2468
Fragments arranged by size:
3259 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI