Restriction map for
pFN21AB6250
(Circular) (Six-base) MAPSORT of: pFN21AB6250.seq Check: 5527 from: 1 to: 6751
pFN21AB6250 6751 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4561 278
Size: 53 83 186 936 3025 2468
Fragments arranged by size:
3025 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4094 1937
Size: 2157 4594
Acc65I G'GTAC_C
Cuts at: 2289 4072 2289
Size: 1783 4968
AclI AA'CG_TT
Cuts at: 1563 4878 5251 1563
Size: 3315 373 3063
Fragments arranged by size:
3315 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 2842 2926 3113 3262 3346 3430 3514
Size: 478 732 84 187 149 84 84 84
Cuts at: 3514 3533 3598 3766 3850 3934 4814 6198 6466
Size: 19 65 168 84 84 880 1384 268
Cuts at: 6466 1632
Size: 1917
Fragments arranged by size:
1917 1384 880 732 478 268 187 168
149 84 84 84 84 84 84 65
19
AfeI AGC'GCT
Cuts at: 3779 3779
Size: 6751
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6554
AflIII A'CryG_T
Cuts at: 1203 3431 5650 6627 1203
Size: 2228 2219 977 1327
Fragments arranged by size:
2228 2219 1327 977
AgeI A'CCGG_T
Cuts at: 5582 5582
Size: 6751
AhdI GACnn_n'nnGTC
Cuts at: 5480 5480
Size: 6751
AleI CACnn'nnGTG
Cuts at: 1166 2481 1166
Size: 1315 5436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6751
AlwNI CAG_nnn'CTG
Cuts at: 4494 6066 4494
Size: 1572 5179
ApaLI G'TGCA_C
Cuts at: 4808 5964 4808
Size: 1156 5595
ApoI r'AATT_y
Cuts at: 1493 4060 4286 4438 6547 1493
Size: 2567 226 152 2109 1697
Fragments arranged by size:
2567 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5305 160
Size: 872 4273 1606
Fragments arranged by size:
4273 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6751
AvaI C'yCGr_G
Cuts at: 1870 1943 4047 4076 1870
Size: 73 2104 29 4545
Fragments arranged by size:
4545 2104 73 29
BamHI G'GATC_C
Cuts at: 4081 4448 4081
Size: 367 6384
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2289 3648 4072 5527 618
Size: 325 217 627 502 1359 424 1455 1842
Fragments arranged by size:
1842 1455 1359 627 502 424 325 217
BanII G_rGCy'C
Cuts at: 1391 2534 2560 3458 3878 3962 4070 1391
Size: 1143 26 898 420 84 108 4072
Fragments arranged by size:
4072 1143 898 420 108 84 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 2615 3856 928
Size: 971 145 571 1241 3823
Fragments arranged by size:
3823 1241 971 571 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4942 4976 1903
Size: 34 3005 34 3678
Fragments arranged by size:
3678 3005 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4643 5853 4643
Size: 1210 5541
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6751
BfrBI ATG'CAT
Cuts at: 3011 3263 3011
Size: 252 6499
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5362 136
Size: 107 122 71 631 605 3690 1525
Fragments arranged by size:
3690 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2322 3460 6746 2322
Size: 1138 3286 2327
Fragments arranged by size:
3286 2327 1138
BlpI GC'TnA_GC
Cuts at: 4168 4168
Size: 6751
Bme1580I G_kGCm'C
Cuts at: 1790 4812 5968 1790
Size: 3022 1156 2573
Fragments arranged by size:
3022 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 6028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4482 5440 442
Size: 416 53 83 399 24 3065 958 1753
Fragments arranged by size:
3065 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2548 2754 3174 3258 3426 3526 3594
Size: 899 410 206 420 84 168 100 68
Cuts at: 3594 3678 3892 5411 1239
Size: 84 214 1519 2579
Fragments arranged by size:
2579 1519 899 420 410 214 206 168
100 84 84 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4876 5741 6039 3946
Size: 930 865 298 4658
Fragments arranged by size:
4658 930 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2143 3826 5414 882
Size: 617 644 1683 1588 2219
Fragments arranged by size:
2219 1683 1588 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 4542 493
Size: 711 3338 2702
Fragments arranged by size:
3338 2702 711
BsaBI GATnn'nnATC
Cuts at: 6745 6745
Size: 6751
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4558 4940 5575 275
Size: 53 83 186 936 3025 382 635 1451
Fragments arranged by size:
3025 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3908 5184 5582 5856 6003 6414 3908
Size: 1276 398 274 147 411 4245
Fragments arranged by size:
4245 1276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2677 2707 3949 3979 1178
Size: 30 165 30 1274 30 1242 30 3950
Fragments arranged by size:
3950 1274 1242 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2078 2224 2790 2855 3107 3191 3359
Size: 889 3 146 566 65 252 84 168
Cuts at: 3359 3695 3947 1186
Size: 336 252 3990
Fragments arranged by size:
3990 889 566 336 252 252 168 146
84 65 3
BseYI C'CCAG_C
Cuts at: 5954 6450 5954
Size: 496 6255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3615 1163
Size: 573 1879 4299
Fragments arranged by size:
4299 1879 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4201 4962 5990 1540
Size: 275 186 2200 761 1028 2301
Fragments arranged by size:
2301 2200 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2534 3458 3878 3962 4812 4897 5968 2534
Size: 924 420 84 850 85 1071 3317
Fragments arranged by size:
3317 1071 924 850 420 85 84
BsmI GAATG_Cn'
Cuts at: 4262 4355 4262
Size: 93 6658
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2534 2560 3458 3878 3962 4070 4812
Size: 399 744 26 898 420 84 108 742
Cuts at: 4812 4897 5968 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 898 744 742 420 399 108
85 84 26
BspEI T'CCGG_A
Cuts at: 6414 6414
Size: 6751
BspHI T'CATG_A
Cuts at: 2299 2968 4640 6370 2299
Size: 669 1672 1730 2680
Fragments arranged by size:
2680 1730 1672 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 4106 844
Size: 1457 1805 3489
Fragments arranged by size:
3489 1805 1457
BsrBI CCG'CTC
Cuts at: 1394 1823 4638 1394
Size: 429 2815 3507
Fragments arranged by size:
3507 2815 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3013 5246 5420 65
Size: 1183 1765 2233 174 1396
Fragments arranged by size:
2233 1765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5395 5582 1700
Size: 110 3585 187 2869
Fragments arranged by size:
3585 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3469 96
Size: 3373 3378
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6751
BssSI C'ACGA_G
Cuts at: 4811 5823 4811
Size: 1012 5739
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6751
BstBI TT'CG_AA
Cuts at: 5555 5555
Size: 6751
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6751
BstXI CCAn_nnnn'nTGG
Cuts at: 3718 3718
Size: 6751
BstYI r'GATC_y
Cuts at: 1972 2322 3460 3791 4081 4448 4833 4850 6302
Size: 350 1138 331 290 367 385 17 1452
Cuts at: 6302 6388 6400 6746 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1138 385 367 350 346 331
290 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2236 2236
Size: 6751
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6012
Fragments arranged by size:
6012 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 4267 5061 5081 2031
Size: 2236 794 20 3701
Fragments arranged by size:
3701 2236 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6751
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2995 3030 529
Size: 35 2431 35 4250
Fragments arranged by size:
4250 2431 35 35
DraI TTT'AAA
Cuts at: 4055 4055
Size: 6751
DraIII CAC_nnn'GTG
Cuts at: 3728 3728
Size: 6751
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5758 809
Size: 507 4442 1802
Fragments arranged by size:
4442 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4198 5087 6716
Size: 54 1425 50 275 18 2368 889 1629
Cuts at: 6716 8
Size: 43
Fragments arranged by size:
2368 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4198 1537
Size: 275 2386 4090
Fragments arranged by size:
4090 2386 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2274 2298 2751 2919 2930 4681 1353
Size: 921 24 453 168 11 1751 3423
Fragments arranged by size:
3423 1751 921 453 168 24 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5338 5712 5858 387
Size: 1064 138 3749 374 146 1280
Fragments arranged by size:
3749 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2532 3456 3876 3960 2532
Size: 924 420 84 5323
Fragments arranged by size:
5323 924 420 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2138 2548 2754 2842 2926 3113
Size: 393 478 28 410 206 88 84 187
Cuts at: 3113 3174 3258 3262 3346 3426 3430 3514 3526
Size: 61 84 4 84 80 4 84 12
Cuts at: 3526 3533 3594 3598 3678 3766 3850 3892 3934
Size: 7 61 4 80 88 84 42 42
Cuts at: 3934 4814 5411 6198 6466 1239
Size: 880 597 787 268 1524
Fragments arranged by size:
1524 880 787 597 478 410 393 268
206 187 88 88 84 84 84 84
84 80 80 61 61 42 42 28
12 7 4 4 4
EcoRI G'AATT_C
Cuts at: 4060 4438 4060
Size: 378 6373
FspI TGC'GCA
Cuts at: 5257 5257
Size: 6751
HaeII r_GCGC'y
Cuts at: 1678 3781 5898 1678
Size: 2103 2117 2531
Fragments arranged by size:
2531 2117 2103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 2283 2334 2366
Size: 32 135 32 647 32 32 51 32
Cuts at: 2366 2440 2472 2521 2553 2677 2689 2709 2721
Size: 74 32 49 32 124 12 20 12
Cuts at: 2721 2857 2889 3445 3477 3949 3981 5392 5424
Size: 136 32 556 32 472 32 1411 32
Cuts at: 5424 5466 5498 1373
Size: 42 32 2626
Fragments arranged by size:
2626 1411 647 556 472 136 135 124
74 51 49 42 32 32 32 32
32 32 32 32 32 32 32 32
20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4095 4341 669
Size: 988 281 2157 246 3079
Fragments arranged by size:
3079 2157 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6751
HpaI GTT'AAC
Cuts at: 4341 4341
Size: 6751
KpnI G_GTAC'C
Cuts at: 2293 4076 2293
Size: 1783 4968
MfeI C'AATT_G
Cuts at: 4350 4350
Size: 6751
MluI A'CGCG_T
Cuts at: 6627 6627
Size: 6751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 3431 3935 5865 6049 1305
Size: 1074 1052 504 1930 184 2007
Fragments arranged by size:
2007 1930 1074 1052 504 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4929
Fragments arranged by size:
4929 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 3001 3169 3253 3589 3757 3841
Size: 648 1315 520 168 84 336 168 84
Cuts at: 3841 3925 3993 4709 5068 5227 518
Size: 84 68 716 359 159 2042
Fragments arranged by size:
2042 1315 716 648 520 359 336 168
168 159 84 84 84 68
MspA1I CmG'CkG
Cuts at: 1606 2526 3870 4167 4844 5992 6237 1606
Size: 920 1344 297 677 1148 245 2120
Fragments arranged by size:
2120 1344 1148 920 677 297 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6199
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6751
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6751
NotI GC'GGCC_GC
Cuts at: 4198 4198
Size: 6751
NsiI A_TGCA'T
Cuts at: 3013 3265 3013
Size: 252 6499
NspI r_CATG'y
Cuts at: 1132 1229 3015 3267 3435 4109 5654 1132
Size: 97 1786 252 168 674 1545 2229
Fragments arranged by size:
2229 1786 1545 674 252 168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6751
PciI A'CATG_T
Cuts at: 3431 5650 3431
Size: 2219 4532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6751
PmeI GTTT'AAAC
Cuts at: 4055 4055
Size: 6751
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4820 4852 6333 6365 4820
Size: 32 1481 32 5206
Fragments arranged by size:
5206 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6751
PsiI TTA'TAA
Cuts at: 4321 4321
Size: 6751
PspXI vC'TCGA_Gb
Cuts at: 4047 4047
Size: 6751
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2434 2466 2434
Size: 32 6719
PstI C_TGCA'G
Cuts at: 830 2964 4103 830
Size: 2134 1139 3478
Fragments arranged by size:
3478 2134 1139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6751
PvuII CAG'CTG
Cuts at: 2526 3870 2526
Size: 1344 5407
SacI G_AGCT'C
Cuts at: 2534 3458 3878 3962 2534
Size: 924 420 84 5323
Fragments arranged by size:
5323 924 420 84
SalI G'TCGA_C
Cuts at: 1936 4093 1936
Size: 2157 4594
SapI GCTCTTCn'nnn_
Cuts at: 2298 2930 2298
Size: 632 6119
SbfI CC_TGCA'GG
Cuts at: 4103 4103
Size: 6751
ScaI AGT'ACT
Cuts at: 4999 6711 4999
Size: 1712 5039
SfcI C'TryA_G
Cuts at: 826 1045 2960 3475 4099 5234 5915 6106 826
Size: 219 1915 515 624 1135 681 191 1471
Fragments arranged by size:
1915 1471 1135 681 624 515 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6751
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6751
SmaI CCC'GGG
Cuts at: 1872 4078 1872
Size: 2206 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4047 4855 5756 6018 820
Size: 197 926 2018 86 808 901 262 1553
Fragments arranged by size:
2018 1553 926 901 808 262 197 86
SnaBI TAC'GTA
Cuts at: 493 4542 493
Size: 4049 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6751
SphI G_CATG'C
Cuts at: 1132 4109 1132
Size: 2977 3774
SspI AAT'ATT
Cuts at: 5 52 2377 4675 5
Size: 47 2325 2298 2081
Fragments arranged by size:
2325 2298 2081 47
StuI AGG'CCT
Cuts at: 1517 3371 3527 1517
Size: 1854 156 4741
Fragments arranged by size:
4741 1854 156
StyI C'CwwG_G
Cuts at: 513 1065 4190 513
Size: 552 3125 3074
Fragments arranged by size:
3125 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4778 4795 4948 5133 5578 325
Size: 1255 3198 17 153 185 445 1498
Fragments arranged by size:
3198 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3469 4997 6709
Size: 274 80 33 51 1443 1492 1528 1712
Cuts at: 6709 96
Size: 138
Fragments arranged by size:
1712 1528 1492 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6711 6743 6711
Size: 32 6719
Tth111I GACn'n_nGTC
Cuts at: 1316 2011 1316
Size: 695 6056
XbaI T'CTAG_A
Cuts at: 4087 4087
Size: 6751
XhoI C'TCGA_G
Cuts at: 1943 4047 1943
Size: 2104 4647
XmaI C'CCGG_G
Cuts at: 1870 4076 1870
Size: 2206 4545
XmnI GAAnn'nnTTC
Cuts at: 2519 2939 3107 3191 3443 3611 3695 3863 3947
Size: 420 168 84 252 168 84 168 84
Cuts at: 3947 4880 2519
Size: 933 4390
Fragments arranged by size:
4390 933 420 252 168 168 168 84
84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4559 276
Size: 53 83 186 936 3025 2468
Fragments arranged by size:
3025 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI