(Circular) (Six-base) MAPSORT of: pFN21AB6249.seq Check: 4523 from: 1 to: 6973
pFN21AB6249 6973 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4783 278
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2613 4316 1937
Size: 676 1703 4594
Fragments arranged by size:
4594 1703 676
Acc65I G'GTAC_C
Cuts at: 4294 4294
Size: 6973
AclI AA'CG_TT
Cuts at: 1563 5100 5473 1563
Size: 3537 373 3063
Fragments arranged by size:
3537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3344 3773 3998 4155 5036 6420 6688 1632
Size: 1712 429 225 157 881 1384 268 1917
Fragments arranged by size:
1917 1712 1384 881 429 268 225 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6776
AflIII A'CryG_T
Cuts at: 1203 2036 5872 6849 1203
Size: 833 3836 977 1327
Fragments arranged by size:
3836 1327 977 833
AgeI A'CCGG_T
Cuts at: 5804 5804
Size: 6973
AhdI GACnn_n'nnGTC
Cuts at: 5702 5702
Size: 6973
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3359 1184
Size: 2175 4798
AlwNI CAG_nnn'CTG
Cuts at: 2569 3061 3322 4716 6288 2569
Size: 492 261 1394 1572 3254
Fragments arranged by size:
3254 1572 1394 492 261
ApaI G_GGCC'C
Cuts at: 2892 2892
Size: 6973
ApaLI G'TGCA_C
Cuts at: 5030 6186 5030
Size: 1156 5817
ApoI r'AATT_y
Cuts at: 1493 3412 4282 4508 4660 6769 1493
Size: 1919 870 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 870 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5527 160
Size: 872 4495 1606
Fragments arranged by size:
4495 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 3738 4298 1870
Size: 73 289 1506 560 4545
Fragments arranged by size:
4545 1506 560 289 73
AvrII C'CTAG_G
Cuts at: 2503 2503
Size: 6973
BamHI G'GATC_C
Cuts at: 2875 4303 4670 2875
Size: 1428 367 5178
Fragments arranged by size:
5178 1428 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2178 2263 2363 2630 2853
Size: 325 217 627 391 85 100 267 223
Cuts at: 2853 2963 4294 5749 618
Size: 110 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 627 391 325 267 223
217 110 100 85
BanII G_rGCy'C
Cuts at: 1391 2154 2892 3255 3573 3656 3966 4292 1391
Size: 763 738 363 318 83 310 326 4072
Fragments arranged by size:
4072 763 738 363 326 318 310 83
BbeI G_GCGC'C
Cuts at: 2267 2267
Size: 6973
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2751 3932 928
Size: 971 852 1181 3969
Fragments arranged by size:
3969 1181 971 852
BbvCI CC'TCA_GC
Cuts at: 3281 3281
Size: 6973
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2530 2564 3083 3117 5164 5198 1903
Size: 34 593 34 519 34 2047 34 3678
Fragments arranged by size:
3678 2047 593 519 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4865 6075 3251
Size: 1614 1210 4149
Fragments arranged by size:
4149 1614 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6973
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3660 5584 136
Size: 107 122 71 631 605 1988 1924 1525
Fragments arranged by size:
1988 1924 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2311 3204 6968 2311
Size: 893 3764 2316
Fragments arranged by size:
3764 2316 893
BlpI GC'TnA_GC
Cuts at: 2153 2814 4390 2153
Size: 661 1576 4736
Fragments arranged by size:
4736 1576 661
Bme1580I G_kGCm'C
Cuts at: 1790 2873 2892 2966 5034 6190 1790
Size: 1083 19 74 2068 1156 2573
Fragments arranged by size:
2573 2068 1156 1083 74 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6973
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4704 5662 442
Size: 416 53 83 399 24 3287 958 1753
Fragments arranged by size:
3287 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2330 4285 2330
Size: 1955 5018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2606 2641 2843 3506 3565 4240 4280
Size: 965 402 35 202 663 59 675 40
Cuts at: 4280 5633 1239
Size: 1353 2579
Fragments arranged by size:
2579 1353 965 675 663 402 202 59
40 35
Bpu10I CC'TnA_GC
Cuts at: 3281 4101 3281
Size: 820 6153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3425 5098 5963 6261 3425
Size: 1673 865 298 4137
Fragments arranged by size:
4137 1673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3735 5636 882
Size: 617 2236 1901 2219
Fragments arranged by size:
2236 2219 1901 617
BsaAI yAC'GTr
Cuts at: 493 1204 2883 4764 493
Size: 711 1679 1881 2702
Fragments arranged by size:
2702 1881 1679 711
BsaBI GATnn'nnATC
Cuts at: 2129 2192 6967 2129
Size: 63 4775 2135
Fragments arranged by size:
4775 2135 63
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2264 4780 5162 5797
Size: 53 83 186 936 731 2516 382 635
Cuts at: 5797 275
Size: 1451
Fragments arranged by size:
2516 1451 936 731 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2316 5406 5804 6078 6225 6636 2316
Size: 3090 398 274 147 411 2653
Fragments arranged by size:
3090 2653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6748
Fragments arranged by size:
6748 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2322 3062 3368 3664 3710 1186
Size: 1136 740 306 296 46 4449
Fragments arranged by size:
4449 1136 740 306 296 46
BseYI C'CCAG_C
Cuts at: 6176 6672 6176
Size: 496 6477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2711 4135 1163
Size: 573 975 1424 4001
Fragments arranged by size:
4001 1424 975 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4423 5184 6212 1540
Size: 275 186 2422 761 1028 2301
Fragments arranged by size:
2422 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 3573 3966 4251 5034 5119 6190 2154
Size: 1419 393 285 783 85 1071 2937
Fragments arranged by size:
2937 1419 1071 783 393 285 85
BsmI GAATG_Cn'
Cuts at: 2698 4039 4484 4577 2698
Size: 1341 445 93 5094
Fragments arranged by size:
5094 1341 445 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2873 2892 2966 3255 3573 3656
Size: 399 364 719 19 74 289 318 83
Cuts at: 3656 3966 4251 4292 5034 5119 6190 1391
Size: 310 285 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 399 364 318 310
289 285 85 83 74 41 19
BspEI T'CCGG_A
Cuts at: 2316 6636 2316
Size: 4320 2653
BspHI T'CATG_A
Cuts at: 2196 2413 4862 6592 2196
Size: 217 2449 1730 2577
Fragments arranged by size:
2577 2449 1730 217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2283 4328 844
Size: 1439 2045 3489
Fragments arranged by size:
3489 2045 1439
BsrBI CCG'CTC
Cuts at: 1394 1823 2173 4860 1394
Size: 429 350 2687 3507
Fragments arranged by size:
3507 2687 429 350
BsrDI GCAATG_nn'
Cuts at: 65 1248 2388 5468 5642 65
Size: 1183 1140 3080 174 1396
Fragments arranged by size:
3080 1396 1183 1140 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2142 3339 5617 5804 1700
Size: 110 332 1197 2278 187 2869
Fragments arranged by size:
2869 2278 1197 332 187 110
BsrGI T'GTAC_A
Cuts at: 96 3714 96
Size: 3618 3355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6973
BssSI C'ACGA_G
Cuts at: 5033 6045 5033
Size: 1012 5961
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2538 2689 2842 794
Size: 1744 151 153 4925
Fragments arranged by size:
4925 1744 153 151
BstBI TT'CG_AA
Cuts at: 5777 5777
Size: 6973
BstEII G'GTnAC_C
Cuts at: 1184 2013 1184
Size: 829 6144
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 2521 2224
Size: 297 6676
BstYI r'GATC_y
Cuts at: 1972 2187 2311 2437 2875 3157 3204 4303 4670
Size: 215 124 126 438 282 47 1099 367
Cuts at: 4670 5055 5072 6524 6610 6622 6968 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1099 438 385 367 346 282
215 126 124 86 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 2244 2244
Size: 6973
BtgI C'CryG_G
Cuts at: 513 1065 2289 3212 3417 3657 4000 4080 513
Size: 552 1224 923 205 240 343 80 3406
Fragments arranged by size:
3406 1224 923 552 343 240 205 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6234
Fragments arranged by size:
6234 683 56
BtsI GCAGTG_nn'
Cuts at: 2144 2699 4489 5283 5303 2144
Size: 555 1790 794 20 3814
Fragments arranged by size:
3814 1790 794 555 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3982 4017 529
Size: 35 3418 35 3485
Fragments arranged by size:
3485 3418 35 35
DraI TTT'AAA
Cuts at: 4277 4277
Size: 6973
DraIII CAC_nnn'GTG
Cuts at: 3111 3111
Size: 6973
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5980 809
Size: 507 4664 1802
Fragments arranged by size:
4664 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 4420 5309
Size: 54 1425 50 275 18 456 2134 889
Cuts at: 5309 6938 8
Size: 1629 43
Fragments arranged by size:
2134 1629 1425 889 456 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4420 1537
Size: 275 2608 4090
Fragments arranged by size:
4090 2608 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2711 3888 4109 4239 4903 1353
Size: 1358 1177 221 130 664 3423
Fragments arranged by size:
3423 1358 1177 664 221 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5560 5934 6080 387
Size: 1064 138 3971 374 146 1280
Fragments arranged by size:
3971 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2152 3571 3964 2152
Size: 1419 393 5161
Fragments arranged by size:
5161 1419 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2606 2641 2843 3344 3506 3565
Size: 393 572 402 35 202 501 162 59
Cuts at: 3565 3773 3998 4155 4240 4280 5036 5633 6420
Size: 208 225 157 85 40 756 597 787
Cuts at: 6420 6688 1239
Size: 268 1524
Fragments arranged by size:
1524 787 756 597 572 501 402 393
268 225 208 202 162 157 85 59
40 35
EcoNI CCTnn'n_nnAGG
Cuts at: 4262 4262
Size: 6973
EcoO109I rG'GnC_Cy
Cuts at: 2043 2556 2908 2043
Size: 513 352 6108
Fragments arranged by size:
6108 513 352
EcoRI G'AATT_C
Cuts at: 3412 4282 4660 3412
Size: 870 378 5725
Fragments arranged by size:
5725 870 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2081 2081
Size: 6973
FspI TGC'GCA
Cuts at: 5479 5479
Size: 6973
HaeII r_GCGC'y
Cuts at: 1678 2065 2267 3872 6120 1678
Size: 387 202 1605 2248 2531
Fragments arranged by size:
2531 2248 1605 387 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 3604 3636 5614
Size: 32 135 32 806 32 1194 32 1978
Cuts at: 5614 5646 5688 5720 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1978 1194 806 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2614 4317 4563 669
Size: 988 281 676 1703 246 3079
Fragments arranged by size:
3079 1703 988 676 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6973
HpaI GTT'AAC
Cuts at: 4563 4563
Size: 6973
KasI G'GCGC_C
Cuts at: 2263 2263
Size: 6973
KpnI G_GTAC'C
Cuts at: 4298 4298
Size: 6973
MfeI C'AATT_G
Cuts at: 4572 4572
Size: 6973
MluI A'CGCG_T
Cuts at: 6849 6849
Size: 6973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3049 3432 4113 6087 6271 1305
Size: 1744 383 681 1974 184 2007
Fragments arranged by size:
2007 1974 1744 681 383 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 10
Size: 54 1425 343 456 4695
Fragments arranged by size:
4695 1425 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 4931 5290 5449 518
Size: 648 1051 2714 359 159 2042
Fragments arranged by size:
2714 2042 1051 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3237 3280 3393 4063 4254 4389 5066 6214
Size: 1631 43 113 670 191 135 677 1148
Cuts at: 6214 6459 1606
Size: 245 2120
Fragments arranged by size:
2120 1631 1148 677 670 245 191 135
113 43
NaeI GCC'GGC
Cuts at: 2144 2144
Size: 6973
NarI GG'CG_CC
Cuts at: 2264 2264
Size: 6973
NcoI C'CATG_G
Cuts at: 513 1065 2289 3417 3657 513
Size: 552 1224 1128 240 3829
Fragments arranged by size:
3829 1224 1128 552 240
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6973
NgoMIV G'CCGG_C
Cuts at: 2142 2142
Size: 6973
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6973
NotI GC'GGCC_GC
Cuts at: 4420 4420
Size: 6973
NspI r_CATG'y
Cuts at: 1132 1229 2040 4331 5876 1132
Size: 97 811 2291 1545 2229
Fragments arranged by size:
2291 2229 1545 811 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6973
PciI A'CATG_T
Cuts at: 2036 5872 2036
Size: 3836 3137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3148 1108
Size: 902 1138 4933
Fragments arranged by size:
4933 1138 902
PmeI GTTT'AAAC
Cuts at: 4277 4277
Size: 6973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5042 5074 6555 6587 5042
Size: 32 1481 32 5428
Fragments arranged by size:
5428 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2556 2556
Size: 6973
PshAI GACnn'nnGTC
Cuts at: 1613 3932 1613
Size: 2319 4654
PsiI TTA'TAA
Cuts at: 4543 4543
Size: 6973
PspOMI G'GGCC_C
Cuts at: 2888 2888
Size: 6973
PstI C_TGCA'G
Cuts at: 830 2466 2565 3006 3069 3348 3627 3945 4325
Size: 1636 99 441 63 279 279 318 380
Cuts at: 4325 830
Size: 3478
Fragments arranged by size:
3478 1636 441 380 318 279 279 99
63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6973
PvuII CAG'CTG
Cuts at: 3237 3280 3393 4063 4254 3237
Size: 43 113 670 191 5956
Fragments arranged by size:
5956 670 191 113 43
SacI G_AGCT'C
Cuts at: 2154 3573 3966 2154
Size: 1419 393 5161
Fragments arranged by size:
5161 1419 393
SalI G'TCGA_C
Cuts at: 1936 2612 4315 1936
Size: 676 1703 4594
Fragments arranged by size:
4594 1703 676
SanDI GG'GwC_CC
Cuts at: 2556 2556
Size: 6973
SapI GCTCTTCn'nnn_
Cuts at: 4239 4239
Size: 6973
SbfI CC_TGCA'GG
Cuts at: 4325 4325
Size: 6973
ScaI AGT'ACT
Cuts at: 2297 5221 6933 2297
Size: 2924 1712 2337
Fragments arranged by size:
2924 2337 1712
SfcI C'TryA_G
Cuts at: 826 1045 2462 2561 3002 3065 3344 3623 3941
Size: 219 1417 99 441 63 279 279 318
Cuts at: 3941 4321 5456 6137 6328 826
Size: 380 1135 681 191 1471
Fragments arranged by size:
1471 1417 1135 681 441 380 318 279
279 219 191 99 63
SfoI GGC'GCC
Cuts at: 2265 2265
Size: 6973
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6973
SmaI CCC'GGG
Cuts at: 1872 4300 1872
Size: 2428 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3440 3738 5077 5978 6240 820
Size: 197 926 1497 298 1339 901 262 1553
Fragments arranged by size:
1553 1497 1339 926 901 298 262 197
SnaBI TAC'GTA
Cuts at: 493 4764 493
Size: 4271 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6973
SphI G_CATG'C
Cuts at: 1132 4331 1132
Size: 3199 3774
SspI AAT'ATT
Cuts at: 5 52 4897 5
Size: 47 4845 2081
Fragments arranged by size:
4845 2081 47
StuI AGG'CCT
Cuts at: 1517 4268 1517
Size: 2751 4222
StyI C'CwwG_G
Cuts at: 513 1065 2065 2289 2302 2503 2514 3417 3657
Size: 552 1000 224 13 201 11 903 240
Cuts at: 3657 3885 4412 513
Size: 228 527 3074
Fragments arranged by size:
3074 1000 903 552 527 240 228 224
201 13 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 5000 5017 5170 5355 5800 325
Size: 1255 2064 1356 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1356 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 2598 3492
Size: 274 80 33 51 1443 318 303 894
Cuts at: 3492 3714 5219 6931 96
Size: 222 1505 1712 138
Fragments arranged by size:
1712 1505 1443 894 318 303 274 222
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6933 6965 6933
Size: 32 6941
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6973
XbaI T'CTAG_A
Cuts at: 4309 4309
Size: 6973
XhoI C'TCGA_G
Cuts at: 1943 3738 1943
Size: 1795 5178
XmaI C'CCGG_G
Cuts at: 1870 4298 1870
Size: 2428 4545
XmnI GAAnn'nnTTC
Cuts at: 5102 5102
Size: 6973
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4781 276
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI
SwaI XcmI