Restriction map for
pFN21AB6246
(Circular) (Six-base) MAPSORT of: pFN21AB6246.seq Check: 5155 from: 1 to: 6745
pFN21AB6246 6745 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2293 2475 2592 3034 3120 3901 2293
Size: 182 117 442 86 781 5137
Fragments arranged by size:
5137 781 442 182 117 86
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3402 4555 278
Size: 53 83 186 936 1866 1153 2468
Fragments arranged by size:
2468 1866 1153 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4088 1937
Size: 2151 4594
Acc65I G'GTAC_C
Cuts at: 4066 4066
Size: 6745
AclI AA'CG_TT
Cuts at: 1563 4872 5245 1563
Size: 3309 373 3063
Fragments arranged by size:
3309 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3127 4808 6192 6460 1632
Size: 1495 1681 1384 268 1917
Fragments arranged by size:
1917 1681 1495 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6548
AflIII A'CryG_T
Cuts at: 1203 5644 6621 1203
Size: 4441 977 1327
Fragments arranged by size:
4441 1327 977
AgeI A'CCGG_T
Cuts at: 5576 5576
Size: 6745
AhdI GACnn_n'nnGTC
Cuts at: 5474 5474
Size: 6745
AleI CACnn'nnGTG
Cuts at: 1166 3674 1166
Size: 2508 4237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2686 3821 1184
Size: 1502 1135 4108
Fragments arranged by size:
4108 1502 1135
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 6713
AlwNI CAG_nnn'CTG
Cuts at: 2233 2368 2554 2584 2608 3058 3154 3257 4488
Size: 135 186 30 24 450 96 103 1231
Cuts at: 4488 6060 2233
Size: 1572 2918
Fragments arranged by size:
2918 1572 1231 450 186 135 103 96
30 24
ApaI G_GGCC'C
Cuts at: 2643 3314 2643
Size: 671 6074
ApaLI G'TGCA_C
Cuts at: 3230 4802 5958 3230
Size: 1572 1156 4017
Fragments arranged by size:
4017 1572 1156
ApoI r'AATT_y
Cuts at: 1493 4054 4280 4432 6541 1493
Size: 2561 226 152 2109 1697
Fragments arranged by size:
2561 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5299 160
Size: 872 4267 1606
Fragments arranged by size:
4267 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
AvaI C'yCGr_G
Cuts at: 1870 1943 2635 2714 3953 3988 4070 1870
Size: 73 692 79 1239 35 82 4545
Fragments arranged by size:
4545 1239 692 82 79 73 35
BamHI G'GATC_C
Cuts at: 4075 4442 4075
Size: 367 6378
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2708 3602 3830 3968
Size: 325 217 627 518 403 894 228 138
Cuts at: 3968 4066 5521 618
Size: 98 1455 1842
Fragments arranged by size:
1842 1455 894 627 518 403 325 228
217 138 98
BanII G_rGCy'C
Cuts at: 1391 2261 2643 3135 3290 3314 3668 4064 1391
Size: 870 382 492 155 24 354 396 4072
Fragments arranged by size:
4072 870 492 396 382 354 155 24
BbeI G_GCGC'C
Cuts at: 3972 3972
Size: 6745
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2967 3948 4008 928
Size: 971 1068 981 60 3665
Fragments arranged by size:
3665 1068 981 971 60
BbvCI CC'TCA_GC
Cuts at: 3560 3698 3812 3560
Size: 138 114 6493
Fragments arranged by size:
6493 138 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4936 4970 1903
Size: 34 2999 34 3678
Fragments arranged by size:
3678 2999 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4637 5847 4637
Size: 1210 5535
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6745
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3104 3737 5356
Size: 107 122 71 631 605 1432 633 1619
Cuts at: 5356 136
Size: 1525
Fragments arranged by size:
1619 1525 1432 633 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3480 6740 3480
Size: 3260 3485
BlpI GC'TnA_GC
Cuts at: 4162 4162
Size: 6745
Bme1580I G_kGCm'C
Cuts at: 1790 2643 2713 3234 3314 3835 4806 5962 1790
Size: 853 70 521 80 521 971 1156 2573
Fragments arranged by size:
2573 1156 971 853 521 521 80 70
BmgBI CAC'GTC
Cuts at: 1318 3826 1318
Size: 2508 4237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2528 3019 3886
Size: 416 53 83 399 24 1111 491 867
Cuts at: 3886 4476 5434 442
Size: 590 958 1753
Fragments arranged by size:
1753 1111 958 867 590 491 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2405 2630 3080 4034 5405 1239
Size: 1142 24 225 450 954 1371 2579
Fragments arranged by size:
2579 1371 1142 954 450 225 24
Bpu10I CC'TnA_GC
Cuts at: 2255 3560 3698 3812 2255
Size: 1305 138 114 5188
Fragments arranged by size:
5188 1305 138 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4870 5735 6033 4870
Size: 865 298 5582
Fragments arranged by size:
5582 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2812 2885 2942 3230 3637 5408 882
Size: 617 1313 73 57 288 407 1771 2219
Fragments arranged by size:
2219 1771 1313 617 407 288 73 57
BsaAI yAC'GTr
Cuts at: 493 1204 4536 493
Size: 711 3332 2702
Fragments arranged by size:
3332 2702 711
BsaBI GATnn'nnATC
Cuts at: 6739 6739
Size: 6745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3399 3969 4552 4934
Size: 53 83 186 936 1866 570 583 382
Cuts at: 4934 5569 275
Size: 635 1451
Fragments arranged by size:
1866 1451 936 635 583 570 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3012 3997 5178 5576 5850 5997 6408 3012
Size: 985 1181 398 274 147 411 3349
Fragments arranged by size:
3349 1181 985 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2196 2226 3316 3346 3773
Size: 30 165 30 793 30 1090 30 427
Cuts at: 3773 3803 1178
Size: 30 4120
Fragments arranged by size:
4120 1090 793 427 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 2903 3163 3166 3346 3749 3864 3935
Size: 1279 438 260 3 180 403 115 71
Cuts at: 3935 4031 4034 1186
Size: 96 3 3897
Fragments arranged by size:
3897 1279 438 403 260 180 115 96
71 3 3
BseYI C'CCAG_C
Cuts at: 2481 2492 2688 3313 3851 3980 5948 6444 2481
Size: 11 196 625 538 129 1968 496 2782
Fragments arranged by size:
2782 1968 625 538 496 196 129 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2172 2174 2321 3071 3098 3551 3929
Size: 573 436 2 147 750 27 453 378
Cuts at: 3929 1163
Size: 3979
Fragments arranged by size:
3979 750 573 453 436 378 147 27
2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2772 3249 4195 4956 5984 1540
Size: 275 186 771 477 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 771 761 477 275 186
BsiHKAI G_wGCw'C
Cuts at: 2261 2859 3000 3234 3712 4806 4891 5962 2261
Size: 598 141 234 478 1094 85 1071 3044
Fragments arranged by size:
3044 1094 1071 598 478 234 141 85
BsmI GAATG_Cn'
Cuts at: 4256 4349 4256
Size: 93 6652
BsmBI CGTCTCn'nnnn_
Cuts at: 3202 3202
Size: 6745
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2643 2713 2859 3000 3135 3234
Size: 399 471 382 70 146 141 135 99
Cuts at: 3234 3290 3314 3668 3712 3835 4064 4806 4891
Size: 56 24 354 44 123 229 742 85
Cuts at: 4891 5962 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 382 354 229
146 141 135 123 99 85 70 56
44 24
BspEI T'CCGG_A
Cuts at: 3997 6408 3997
Size: 2411 4334
BspHI T'CATG_A
Cuts at: 3376 4634 6364 3376
Size: 1258 1730 3757
Fragments arranged by size:
3757 1730 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2293 2475 2592 2601 2691 3034 3120 3901
Size: 1449 182 117 9 90 343 86 781
Cuts at: 3901 4100 844
Size: 199 3489
Fragments arranged by size:
3489 1449 781 343 199 182 117 90
86 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2795 3088 3625 4632 1394
Size: 429 972 293 537 1007 3507
Fragments arranged by size:
3507 1007 972 537 429 293
BsrDI GCAATG_nn'
Cuts at: 65 1248 2167 5240 5414 65
Size: 1183 919 3073 174 1396
Fragments arranged by size:
3073 1396 1183 919 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5389 5576 1700
Size: 110 3579 187 2869
Fragments arranged by size:
3579 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6745
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6745
BssSI C'ACGA_G
Cuts at: 2885 3227 4805 5817 2885
Size: 342 1578 1012 3813
Fragments arranged by size:
3813 1578 1012 342
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2233 2356 2392 2554 2569 2584 3049 3716
Size: 1439 123 36 162 15 15 465 667
Cuts at: 3716 794
Size: 3823
Fragments arranged by size:
3823 1439 667 465 162 123 36 15
15
BstBI TT'CG_AA
Cuts at: 5549 5549
Size: 6745
BstEII G'GTnAC_C
Cuts at: 1184 3190 1184
Size: 2006 4739
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6745
BstYI r'GATC_y
Cuts at: 1972 2364 3063 3480 4075 4442 4827 4844 6296
Size: 392 699 417 595 367 385 17 1452
Cuts at: 6296 6382 6394 6740 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 699 595 417 392 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2248 2248
Size: 6745
BtgI C'CryG_G
Cuts at: 513 1065 2145 3837 513
Size: 552 1080 1692 3421
Fragments arranged by size:
3421 1692 1080 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6006
Fragments arranged by size:
6006 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 3525 4261 5055 5075 3337
Size: 188 736 794 20 5007
Fragments arranged by size:
5007 794 736 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6745
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6710
DraI TTT'AAA
Cuts at: 4049 4049
Size: 6745
DraIII CAC_nnn'GTG
Cuts at: 3907 3907
Size: 6745
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2035 5752 809
Size: 507 719 3717 1802
Fragments arranged by size:
3717 1802 719 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2769 2936
Size: 54 1425 50 275 18 489 450 167
Cuts at: 2936 3246 3738 4192 5081 6710 8
Size: 310 492 454 889 1629 43
Fragments arranged by size:
1629 1425 889 492 489 454 450 310
275 167 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2769 3246 4192 1537
Size: 275 957 477 946 4090
Fragments arranged by size:
4090 957 946 477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2884 4675 1353
Size: 1531 1791 3423
Fragments arranged by size:
3423 1791 1531
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3422 3781 5332 5706 5852 387
Size: 1064 138 1833 359 1551 374 146 1280
Fragments arranged by size:
1833 1551 1280 1064 374 359 146 138
EcoICRI GAG'CTC
Cuts at: 2259 2259
Size: 6745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2405 2630 3080 3127 4034 4808
Size: 393 749 24 225 450 47 907 774
Cuts at: 4808 5405 6192 6460 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 907 787 774 749 597 450 393
268 225 47 24
EcoNI CCTnn'n_nnAGG
Cuts at: 4016 4016
Size: 6745
EcoO109I rG'GnC_Cy
Cuts at: 2639 2640 3310 3992 4009 2639
Size: 1 670 682 17 5375
Fragments arranged by size:
5375 682 670 17 1
EcoRI G'AATT_C
Cuts at: 4054 4432 4054
Size: 378 6367
FspI TGC'GCA
Cuts at: 5251 5251
Size: 6745
HaeII r_GCGC'y
Cuts at: 1678 3972 3987 5892 1678
Size: 2294 15 1905 2531
Fragments arranged by size:
2531 2294 1905 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5386 5418 5460 5492 1373
Size: 32 135 32 3814 32 42 32 2626
Fragments arranged by size:
3814 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4089 4335 669
Size: 988 281 2151 246 3079
Fragments arranged by size:
3079 2151 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6745
HpaI GTT'AAC
Cuts at: 4335 4335
Size: 6745
KasI G'GCGC_C
Cuts at: 3968 3968
Size: 6745
KpnI G_GTAC'C
Cuts at: 4070 4070
Size: 6745
MfeI C'AATT_G
Cuts at: 4344 4344
Size: 6745
MluI A'CGCG_T
Cuts at: 6621 6621
Size: 6745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3302 5859 6043 1305
Size: 1997 2557 184 2007
Fragments arranged by size:
2557 2007 1997 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 2938 10
Size: 54 1425 343 489 617 3817
Fragments arranged by size:
3817 1425 617 489 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3500 3674 4703 5062 5221 518
Size: 648 843 1491 174 1029 359 159 2042
Fragments arranged by size:
2042 1491 1029 843 648 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 2097 2383 2533 2666 2692 3058 3466 3847
Size: 491 286 150 133 26 366 408 381
Cuts at: 3847 4161 4838 5986 6231 1606
Size: 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 491 408 381 366 314
286 245 150 133 26
NarI GG'CG_CC
Cuts at: 3969 3969
Size: 6745
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6193
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6745
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6745
NotI GC'GGCC_GC
Cuts at: 4192 4192
Size: 6745
NspI r_CATG'y
Cuts at: 1132 1229 3121 3778 4103 5648 1132
Size: 97 1892 657 325 1545 2229
Fragments arranged by size:
2229 1892 1545 657 325 97
PasI CC'CwG_GG
Cuts at: 1348 2264 3964 1348
Size: 916 1700 4129
Fragments arranged by size:
4129 1700 916
PciI A'CATG_T
Cuts at: 5644 5644
Size: 6745
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6745
PfoI T'CCnGG_A
Cuts at: 3444 3444
Size: 6745
PmeI GTTT'AAAC
Cuts at: 4049 4049
Size: 6745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3773 3805 4814 4846 6327 6359 3674
Size: 32 67 32 1009 32 1481 32 4060
Fragments arranged by size:
4060 1481 1009 67 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4009 4009
Size: 6745
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6745
PsiI TTA'TAA
Cuts at: 4315 4315
Size: 6745
PspOMI G'GGCC_C
Cuts at: 2639 3310 2639
Size: 671 6074
PstI C_TGCA'G
Cuts at: 830 2241 2388 2472 2598 2622 3096 3205 4097
Size: 1411 147 84 126 24 474 109 892
Cuts at: 4097 830
Size: 3478
Fragments arranged by size:
3478 1411 892 474 147 126 109 84
24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6745
PvuII CAG'CTG
Cuts at: 2383 2533 2666 2692 3058 3466 2383
Size: 150 133 26 366 408 5662
Fragments arranged by size:
5662 408 366 150 133 26
SacI G_AGCT'C
Cuts at: 2261 2261
Size: 6745
SalI G'TCGA_C
Cuts at: 1936 4087 1936
Size: 2151 4594
SapI GCTCTTCn'nnn_
Cuts at: 2884 2884
Size: 6745
SbfI CC_TGCA'GG
Cuts at: 4097 4097
Size: 6745
ScaI AGT'ACT
Cuts at: 2438 4993 6705 2438
Size: 2555 1712 2478
Fragments arranged by size:
2555 2478 1712
SexAI A'CCwGG_T
Cuts at: 3903 3903
Size: 6745
SfcI C'TryA_G
Cuts at: 826 1045 2237 2384 2429 2468 2594 2618 3092
Size: 219 1192 147 45 39 126 24 474
Cuts at: 3092 3201 4093 5228 5909 6100 826
Size: 109 892 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 892 681 474 219 191
147 126 109 45 39 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 3104 3737 3104
Size: 633 6112
SfoI GGC'GCC
Cuts at: 3970 3970
Size: 6745
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6745
SmaI CCC'GGG
Cuts at: 1872 2716 3955 4072 1872
Size: 844 1239 117 4545
Fragments arranged by size:
4545 1239 844 117
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4849 5750 6012 820
Size: 197 926 2906 901 262 1553
Fragments arranged by size:
2906 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4536 493
Size: 4043 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6745
SphI G_CATG'C
Cuts at: 1132 3778 4103 1132
Size: 2646 325 3774
Fragments arranged by size:
3774 2646 325
SrfI GCCC'GGGC
Cuts at: 3955 3955
Size: 6745
SspI AAT'ATT
Cuts at: 5 52 4669 5
Size: 47 4617 2081
Fragments arranged by size:
4617 2081 47
StuI AGG'CCT
Cuts at: 1517 2246 2253 2390 1517
Size: 729 7 137 5872
Fragments arranged by size:
5872 729 137 7
StyI C'CwwG_G
Cuts at: 513 1065 2802 3075 3186 4184 513
Size: 552 1737 273 111 998 3074
Fragments arranged by size:
3074 1737 998 552 273 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2495 3818 4772 4789 4942 5127 5572
Size: 1255 915 1323 954 17 153 185 445
Cuts at: 5572 325
Size: 1498
Fragments arranged by size:
1498 1323 1255 954 915 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2436 3017 3894
Size: 274 80 33 51 1443 459 581 877
Cuts at: 3894 4991 6703 96
Size: 1097 1712 138
Fragments arranged by size:
1712 1443 1097 877 581 459 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3316 3348 6705 6737 3316
Size: 32 3357 32 3324
Fragments arranged by size:
3357 3324 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2735 1316
Size: 1419 5326
XbaI T'CTAG_A
Cuts at: 4081 4081
Size: 6745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2489 2515 2489
Size: 26 6719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6745
XmaI C'CCGG_G
Cuts at: 1870 2714 3953 4070 1870
Size: 844 1239 117 4545
Fragments arranged by size:
4545 1239 844 117
XmnI GAAnn'nnTTC
Cuts at: 4874 4874
Size: 6745
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3400 4553 276
Size: 53 83 186 936 1866 1153 2468
Fragments arranged by size:
2468 1866 1153 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SwaI