Restriction map for
pFN21AB6245
(Circular) (Six-base) MAPSORT of: pFN21AB6245.seq Check: 3835 from: 1 to: 6964
pFN21AB6245 6964 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2209 2224 3412 3433 2209
Size: 15 1188 21 5740
Fragments arranged by size:
5740 1188 21 15
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4774 278
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3508 3777 4307 1937
Size: 1571 269 530 4594
Fragments arranged by size:
4594 1571 530 269
Acc65I G'GTAC_C
Cuts at: 3281 3969 4285 3281
Size: 688 316 5960
Fragments arranged by size:
5960 688 316
AclI AA'CG_TT
Cuts at: 1563 5091 5464 1563
Size: 3528 373 3063
Fragments arranged by size:
3528 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2829 2974 3603 5027 6411 6679 1632
Size: 1197 145 629 1424 1384 268 1917
Fragments arranged by size:
1917 1424 1384 1197 629 268 145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6767
AflIII A'CryG_T
Cuts at: 1203 3772 5863 6840 1203
Size: 2569 2091 977 1327
Fragments arranged by size:
2569 2091 1327 977
AgeI A'CCGG_T
Cuts at: 2477 5795 2477
Size: 3318 3646
AhdI GACnn_n'nnGTC
Cuts at: 5693 5693
Size: 6964
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6964
AlwNI CAG_nnn'CTG
Cuts at: 2395 2428 4707 6279 2395
Size: 33 2279 1572 3080
Fragments arranged by size:
3080 2279 1572 33
ApaI G_GGCC'C
Cuts at: 2436 2818 2436
Size: 382 6582
ApaLI G'TGCA_C
Cuts at: 5021 6177 5021
Size: 1156 5808
ApoI r'AATT_y
Cuts at: 1493 3203 3922 4054 4273 4499 4651 6760 1493
Size: 1710 719 132 219 226 152 2109 1697
Fragments arranged by size:
2109 1710 1697 719 226 219 152 132
AseI AT'TA_AT
Cuts at: 160 1032 5518 160
Size: 872 4486 1606
Fragments arranged by size:
4486 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2372 2460 2516 2654 2666 4289
Size: 73 78 351 88 56 138 12 1623
Cuts at: 4289 1870
Size: 4545
Fragments arranged by size:
4545 1623 351 138 88 78 73 56
12
AvrII C'CTAG_G
Cuts at: 3698 3698
Size: 6964
BamHI G'GATC_C
Cuts at: 2781 2820 3051 3499 4294 4661 2781
Size: 39 231 448 795 367 5084
Fragments arranged by size:
5084 795 448 367 231 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2357 2750 3281 3969
Size: 325 217 627 294 276 393 531 688
Cuts at: 3969 4285 5740 618
Size: 316 1455 1842
Fragments arranged by size:
1842 1455 688 627 531 393 325 316
294 276 217
BanII G_rGCy'C
Cuts at: 1391 2112 2436 2694 2818 2839 3440 4283 1391
Size: 721 324 258 124 21 601 843 4072
Fragments arranged by size:
4072 843 721 601 324 258 124 21
BbeI G_GCGC'C
Cuts at: 2361 2361
Size: 6964
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3969 4162 928
Size: 971 2070 193 3730
Fragments arranged by size:
3730 2070 971 193
BbvCI CC'TCA_GC
Cuts at: 2034 4140 2034
Size: 2106 4858
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3919 3953 5155 5189 1903
Size: 34 1982 34 1202 34 3678
Fragments arranged by size:
3678 1982 1202 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4856 6066 4856
Size: 1210 5754
BclI T'GATC_A
Cuts at: 1548 2637 1548
Size: 1089 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5575 136
Size: 107 122 71 631 605 3903 1525
Fragments arranged by size:
3903 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6959 6959
Size: 6964
BlpI GC'TnA_GC
Cuts at: 4381 4381
Size: 6964
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2436 2818 5025 6181 1790
Size: 294 352 382 2207 1156 2573
Fragments arranged by size:
2573 2207 1156 382 352 294
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6964
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3555 4695 5653
Size: 416 53 83 399 24 2138 1140 958
Cuts at: 5653 442
Size: 1753
Fragments arranged by size:
2138 1753 1140 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 6964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2350 2476 2582 2930 3457 5624 1239
Size: 1111 126 106 348 527 2167 2579
Fragments arranged by size:
2579 2167 1111 527 348 126 106
Bpu10I CC'TnA_GC
Cuts at: 2034 3483 4140 4253 2034
Size: 1449 657 113 4745
Fragments arranged by size:
4745 1449 657 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4015 5089 5954 6252 4015
Size: 1074 865 298 4727
Fragments arranged by size:
4727 1074 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5627 882
Size: 617 4128 2219
Fragments arranged by size:
4128 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3342 4755 493
Size: 711 2138 1413 2702
Fragments arranged by size:
2702 2138 1413 711
BsaBI GATnn'nnATC
Cuts at: 6958 6958
Size: 6964
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2183 2358 4771 5153
Size: 53 83 186 936 650 175 2413 382
Cuts at: 5153 5788 275
Size: 635 1451
Fragments arranged by size:
2413 1451 936 650 635 382 186 175
83 53
BsaWI w'CCGG_w
Cuts at: 2477 2747 5397 5795 6069 6216 6627 2477
Size: 270 2650 398 274 147 411 2814
Fragments arranged by size:
2814 2650 411 398 274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 4174 4204 1178
Size: 30 165 30 1278 30 1463 30 3938
Fragments arranged by size:
3938 1463 1278 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2585 2654 4247 1186
Size: 1399 69 1593 3903
Fragments arranged by size:
3903 1593 1399 69
BseYI C'CCAG_C
Cuts at: 2428 2435 3082 3359 3464 6167 6663 2428
Size: 7 647 277 105 2703 496 2729
Fragments arranged by size:
2729 2703 647 496 277 105 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 1163
Size: 573 672 5719
Fragments arranged by size:
5719 672 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 4414 5175 6203 1540
Size: 275 186 75 2338 761 1028 2301
Fragments arranged by size:
2338 2301 1028 761 275 186 75
BsiHKAI G_wGCw'C
Cuts at: 2839 3739 4254 5025 5110 6181 2839
Size: 900 515 771 85 1071 3622
Fragments arranged by size:
3622 1071 900 771 515 85
BsmI GAATG_Cn'
Cuts at: 3577 3950 4043 4475 4568 3577
Size: 373 93 432 93 5973
Fragments arranged by size:
5973 432 373 93 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2127 3475 2127
Size: 1348 5616
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2112 2436 2694 2818 2839 3440
Size: 399 294 28 324 258 124 21 601
Cuts at: 3440 3739 4254 4283 5025 5110 6181 1391
Size: 299 515 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 515 399 324 299
294 258 124 85 29 28 21
BspEI T'CCGG_A
Cuts at: 6627 6627
Size: 6964
BspHI T'CATG_A
Cuts at: 2577 4853 6583 2577
Size: 2276 1730 2958
Fragments arranged by size:
2958 2276 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2209 2224 2331 3412 3433 4319 844
Size: 1365 15 107 1081 21 886 3489
Fragments arranged by size:
3489 1365 1081 886 107 21 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2057 2947 3333 4851 1394
Size: 429 234 890 386 1518 3507
Fragments arranged by size:
3507 1518 890 429 386 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 5459 5633 65
Size: 1183 4211 174 1396
Fragments arranged by size:
4211 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2071 2477 2521 5608 5795 1700
Size: 110 261 406 44 3087 187 2869
Fragments arranged by size:
3087 2869 406 261 187 110 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6964
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6964
BssSI C'ACGA_G
Cuts at: 5024 6036 5024
Size: 1012 5952
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2147 2239 2386 3659 794
Size: 1353 92 147 1273 4099
Fragments arranged by size:
4099 1353 1273 147 92
BstBI TT'CG_AA
Cuts at: 5768 5768
Size: 6964
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6964
BstXI CCAn_nnnn'nTGG
Cuts at: 2707 2707
Size: 6964
BstYI r'GATC_y
Cuts at: 1972 2781 2820 3051 3499 4294 4661 5046 5063
Size: 809 39 231 448 795 367 385 17
Cuts at: 5063 6515 6601 6613 6959 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 809 795 448 385 367 346
231 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2256 2777 2256
Size: 521 6443
BtgI C'CryG_G
Cuts at: 513 1065 2004 2103 2199 513
Size: 552 939 99 96 5278
Fragments arranged by size:
5278 939 552 99 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6225
Fragments arranged by size:
6225 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 4480 5274 5294 2184
Size: 2296 794 20 3854
Fragments arranged by size:
3854 2296 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6929
DraI TTT'AAA
Cuts at: 4002 4268 4002
Size: 266 6698
DraIII CAC_nnn'GTG
Cuts at: 2386 3598 4049 2386
Size: 1212 451 5301
Fragments arranged by size:
5301 1212 451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5971 809
Size: 507 4655 1802
Fragments arranged by size:
4655 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3796 4411 5300
Size: 54 1425 50 275 18 1966 615 889
Cuts at: 5300 6929 8
Size: 1629 43
Fragments arranged by size:
1966 1629 1425 889 615 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4411 1537
Size: 275 2599 4090
Fragments arranged by size:
4090 2599 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4894 1353
Size: 3541 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2049 2582 2829 5551 5925 6071
Size: 1064 138 460 533 247 2722 374 146
Cuts at: 6071 387
Size: 1280
Fragments arranged by size:
2722 1280 1064 533 460 374 247 146
138
EcoICRI GAG'CTC
Cuts at: 2837 2837
Size: 6964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2350 2476 2582 2829 2930 2974 3457
Size: 393 718 126 106 247 101 44 483
Cuts at: 3457 3603 5027 5624 6411 6679 1239
Size: 146 1424 597 787 268 1524
Fragments arranged by size:
1524 1424 787 718 597 483 393 268
247 146 126 106 101 44
EcoNI CCTnn'n_nnAGG
Cuts at: 3231 3231
Size: 6964
EcoO109I rG'GnC_Cy
Cuts at: 2432 2456 2513 2814 2869 2432
Size: 24 57 301 55 6527
Fragments arranged by size:
6527 301 57 55 24
EcoRI G'AATT_C
Cuts at: 4273 4651 4273
Size: 378 6586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3852 3852
Size: 6964
FseI GG_CCGG'CC
Cuts at: 2525 2525
Size: 6964
FspI TGC'GCA
Cuts at: 5470 5470
Size: 6964
HaeII r_GCGC'y
Cuts at: 1678 2361 2402 3633 6111 1678
Size: 683 41 1231 2478 2531
Fragments arranged by size:
2531 2478 1231 683 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2454 2486 2681 2713 4174
Size: 32 135 32 882 32 195 32 1461
Cuts at: 4174 4206 5605 5637 5679 5711 1373
Size: 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1461 1399 882 195 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4308 4554 669
Size: 988 281 2370 246 3079
Fragments arranged by size:
3079 2370 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6964
HpaI GTT'AAC
Cuts at: 4554 4554
Size: 6964
KasI G'GCGC_C
Cuts at: 2357 2357
Size: 6964
KpnI G_GTAC'C
Cuts at: 3285 3973 4289 3285
Size: 688 316 5960
Fragments arranged by size:
5960 688 316
MfeI C'AATT_G
Cuts at: 4563 4563
Size: 6964
MluI A'CGCG_T
Cuts at: 6840 6840
Size: 6964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 3715 6078 6262 1305
Size: 1182 1228 2363 184 2007
Fragments arranged by size:
2363 2007 1228 1182 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3798 10
Size: 54 1425 343 1966 3176
Fragments arranged by size:
3176 1966 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3771 4922 5281 5440 518
Size: 648 2605 1151 359 159 2042
Fragments arranged by size:
2605 2042 1151 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2201 2279 3415 3574 4144 4380 5057
Size: 462 133 78 1136 159 570 236 677
Cuts at: 5057 6205 6450 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1136 677 570 462 245 236
159 133 78
NaeI GCC'GGC
Cuts at: 2523 2523
Size: 6964
NarI GG'CG_CC
Cuts at: 2358 2358
Size: 6964
NcoI C'CATG_G
Cuts at: 513 1065 2004 2103 513
Size: 552 939 99 5374
Fragments arranged by size:
5374 939 552 99
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6964
NgoMIV G'CCGG_C
Cuts at: 2521 2521
Size: 6964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6964
NotI GC'GGCC_GC
Cuts at: 4411 4411
Size: 6964
NspI r_CATG'y
Cuts at: 1132 1229 2408 3776 3891 4322 5867 1132
Size: 97 1179 1368 115 431 1545 2229
Fragments arranged by size:
2229 1545 1368 1179 431 115 97
PasI CC'CwG_GG
Cuts at: 1348 2810 1348
Size: 1462 5502
PciI A'CATG_T
Cuts at: 3772 5863 3772
Size: 2091 4873
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6964
PfoI T'CCnGG_A
Cuts at: 3495 3495
Size: 6964
PmeI GTTT'AAAC
Cuts at: 4268 4268
Size: 6964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5033 5065 6546 6578 5033
Size: 32 1481 32 5419
Fragments arranged by size:
5419 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2869 2869
Size: 6964
PshAI GACnn'nnGTC
Cuts at: 1613 2867 1613
Size: 1254 5710
PsiI TTA'TAA
Cuts at: 4534 4534
Size: 6964
PspOMI G'GGCC_C
Cuts at: 2432 2814 2432
Size: 382 6582
PspXI vC'TCGA_Gb
Cuts at: 2460 2666 2460
Size: 206 6758
PstI C_TGCA'G
Cuts at: 830 2220 2337 2678 3430 4316 830
Size: 1390 117 341 752 886 3478
Fragments arranged by size:
3478 1390 886 752 341 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6964
PvuII CAG'CTG
Cuts at: 3415 3574 4144 3415
Size: 159 570 6235
Fragments arranged by size:
6235 570 159
SacI G_AGCT'C
Cuts at: 2839 2839
Size: 6964
SacII CC_GC'GG
Cuts at: 2202 2202
Size: 6964
SalI G'TCGA_C
Cuts at: 1936 4306 1936
Size: 2370 4594
SbfI CC_TGCA'GG
Cuts at: 4316 4316
Size: 6964
ScaI AGT'ACT
Cuts at: 3658 5212 6924 3658
Size: 1554 1712 3698
Fragments arranged by size:
3698 1712 1554
SfcI C'TryA_G
Cuts at: 826 1045 2216 2333 2674 3365 3426 4312 5447
Size: 219 1171 117 341 691 61 886 1135
Cuts at: 5447 6128 6319 826
Size: 681 191 1471
Fragments arranged by size:
1471 1171 1135 886 691 681 341 219
191 117 61
SfoI GGC'GCC
Cuts at: 2359 2359
Size: 6964
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6964
SmaI CCC'GGG
Cuts at: 1872 2518 4291 1872
Size: 646 1773 4545
Fragments arranged by size:
4545 1773 646
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2460 2666 4030 5068 5969 6231
Size: 197 926 517 206 1364 1038 901 262
Cuts at: 6231 820
Size: 1553
Fragments arranged by size:
1553 1364 1038 926 901 517 262 206
197
SnaBI TAC'GTA
Cuts at: 493 3342 4755 493
Size: 2849 1413 2702
Fragments arranged by size:
2849 2702 1413
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6964
SphI G_CATG'C
Cuts at: 1132 4322 1132
Size: 3190 3774
SspI AAT'ATT
Cuts at: 5 52 4888 5
Size: 47 4836 2081
Fragments arranged by size:
4836 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6964
StyI C'CwwG_G
Cuts at: 513 1065 2004 2103 2154 2865 3698 3793 4158
Size: 552 939 99 51 711 833 95 365
Cuts at: 4158 4403 513
Size: 245 3074
Fragments arranged by size:
3074 939 833 711 552 365 245 99
95 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3783 4991 5008 5161 5346 5791 325
Size: 1255 2203 1208 17 153 185 445 1498
Fragments arranged by size:
2203 1498 1255 1208 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3656 3884 5210
Size: 274 80 33 51 1443 1679 228 1326
Cuts at: 5210 6922 96
Size: 1712 138
Fragments arranged by size:
1712 1679 1443 1326 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3393 3414 3425 3446 6924 6956 3393
Size: 21 11 21 3478 32 3401
Fragments arranged by size:
3478 3401 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 2347 1316
Size: 1031 5933
XbaI T'CTAG_A
Cuts at: 3622 4300 3622
Size: 678 6286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 3542 2152
Size: 1390 5574
XhoI C'TCGA_G
Cuts at: 1943 2460 2666 1943
Size: 517 206 6241
Fragments arranged by size:
6241 517 206
XmaI C'CCGG_G
Cuts at: 1870 2516 4289 1870
Size: 646 1773 4545
Fragments arranged by size:
4545 1773 646
XmnI GAAnn'nnTTC
Cuts at: 4169 5093 4169
Size: 924 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4772 276
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FspAI NruI NsiI PacI PmlI PsrI RsrII SanDI
SapI SexAI SfiI SrfI SwaI