Restriction map for
pFN21AB6242
(Circular) (Six-base) MAPSORT of: pFN21AB6242.seq Check: 7225 from: 1 to: 6766
pFN21AB6242 6766 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3088 3088
Size: 6766
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4576 278
Size: 53 83 186 936 3040 2468
Fragments arranged by size:
3040 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3056 3962 4109 1937
Size: 1119 906 147 4594
Fragments arranged by size:
4594 1119 906 147
Acc65I G'GTAC_C
Cuts at: 3759 4087 3759
Size: 328 6438
AclI AA'CG_TT
Cuts at: 1563 4893 5266 1563
Size: 3330 373 3063
Fragments arranged by size:
3330 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3251 3458 4050 4829 6213 6481 1632
Size: 1619 207 592 779 1384 268 1917
Fragments arranged by size:
1917 1619 1384 779 592 268 207
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6569
AflIII A'CryG_T
Cuts at: 1203 5665 6642 1203
Size: 4462 977 1327
Fragments arranged by size:
4462 1327 977
AgeI A'CCGG_T
Cuts at: 5597 5597
Size: 6766
AhdI GACnn_n'nnGTC
Cuts at: 3958 5495 3958
Size: 1537 5229
AleI CACnn'nnGTG
Cuts at: 1166 2407 1166
Size: 1241 5525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6766
AlwNI CAG_nnn'CTG
Cuts at: 2464 4509 6081 2464
Size: 2045 1572 3149
Fragments arranged by size:
3149 2045 1572
ApaLI G'TGCA_C
Cuts at: 3485 4823 5979 3485
Size: 1338 1156 4272
Fragments arranged by size:
4272 1338 1156
ApoI r'AATT_y
Cuts at: 1493 2179 2673 2952 3295 3836 3969 4075 4301
Size: 686 494 279 343 541 133 106 226
Cuts at: 4301 4453 6562 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 686 541 494 343 279 226
152 133 106
AseI AT'TA_AT
Cuts at: 160 1032 5320 160
Size: 872 4288 1606
Fragments arranged by size:
4288 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6766
AvaI C'yCGr_G
Cuts at: 1870 1943 4091 1870
Size: 73 2148 4545
Fragments arranged by size:
4545 2148 73
BamHI G'GATC_C
Cuts at: 2020 2137 2156 4096 4463 2020
Size: 117 19 1940 367 4323
Fragments arranged by size:
4323 1940 367 117 19
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 3759 4087 5542 618
Size: 325 217 627 251 1721 328 1455 1842
Fragments arranged by size:
1842 1721 1455 627 328 325 251 217
BanII G_rGCy'C
Cuts at: 1391 2586 3785 4085 1391
Size: 1195 1199 300 4072
Fragments arranged by size:
4072 1199 1195 300
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2846 3000 3401 3918 928
Size: 971 947 154 401 517 3776
Fragments arranged by size:
3776 971 947 517 401 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4957 4991 1903
Size: 34 3020 34 3678
Fragments arranged by size:
3678 3020 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2789 4658 5868 2789
Size: 1869 1210 3687
Fragments arranged by size:
3687 1869 1210
BclI T'GATC_A
Cuts at: 1548 3180 1548
Size: 1632 5134
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5377 136
Size: 107 122 71 631 605 3705 1525
Fragments arranged by size:
3705 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3496 6761 3496
Size: 3265 3501
BlpI GC'TnA_GC
Cuts at: 4183 4183
Size: 6766
Bme1580I G_kGCm'C
Cuts at: 1790 3489 4827 5983 1790
Size: 1699 1338 1156 2573
Fragments arranged by size:
2573 1699 1338 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6766
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4497 5455 442
Size: 416 53 83 399 24 3080 958 1753
Fragments arranged by size:
3080 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6766
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 3804 2566
Size: 1238 5528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 3450 3506 5426 1239
Size: 1262 949 56 1920 2579
Fragments arranged by size:
2579 1920 1262 949 56
Bpu10I CC'TnA_GC
Cuts at: 2319 2319
Size: 6766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3933 4891 5756 6054 3933
Size: 958 865 298 4645
Fragments arranged by size:
4645 958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2468 2507 3570 5429 882
Size: 617 969 39 1063 1859 2219
Fragments arranged by size:
2219 1859 1063 969 617 39
BsaAI yAC'GTr
Cuts at: 493 1204 4557 493
Size: 711 3353 2702
Fragments arranged by size:
3353 2702 711
BsaBI GATnn'nnATC
Cuts at: 3150 6760 3150
Size: 3610 3156
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3717 4573 4955 5590
Size: 53 83 186 936 2184 856 382 635
Cuts at: 5590 275
Size: 1451
Fragments arranged by size:
2184 1451 936 856 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3546 5199 5597 5871 6018 6429 3546
Size: 1653 398 274 147 411 3883
Fragments arranged by size:
3883 1653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 3080 3110 1178
Size: 30 165 30 619 30 1028 30 4834
Fragments arranged by size:
4834 1028 619 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2257 2462 2498 3572 3824 4010 1186
Size: 1071 205 36 1074 252 186 3942
Fragments arranged by size:
3942 1074 1071 252 205 186 36
BseYI C'CCAG_C
Cuts at: 5969 6465 5969
Size: 496 6270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3513 3777 1163
Size: 573 1777 264 4152
Fragments arranged by size:
4152 1777 573 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4216 4977 6005 1540
Size: 275 186 2215 761 1028 2301
Fragments arranged by size:
2301 2215 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2389 2586 3489 3770 4827 4912 5983 2389
Size: 197 903 281 1057 85 1071 3172
Fragments arranged by size:
3172 1071 1057 903 281 197 85
BsmI GAATG_Cn'
Cuts at: 4277 4370 4277
Size: 93 6673
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2389 2586 3489 3770 3785 4085 4827
Size: 399 599 197 903 281 15 300 742
Cuts at: 4827 4912 5983 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 903 742 599 399 300 281
197 85 15
BspEI T'CCGG_A
Cuts at: 6429 6429
Size: 6766
BspHI T'CATG_A
Cuts at: 4655 6385 4655
Size: 1730 5036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3088 4121 844
Size: 2244 1033 3489
Fragments arranged by size:
3489 2244 1033
BsrBI CCG'CTC
Cuts at: 1394 1823 4653 1394
Size: 429 2830 3507
Fragments arranged by size:
3507 2830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2271 5261 5435 65
Size: 1183 1023 2990 174 1396
Fragments arranged by size:
2990 1396 1183 1023 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2889 3816 5410 5597 1700
Size: 110 1079 927 1594 187 2869
Fragments arranged by size:
2869 1594 1079 927 187 110
BsrGI T'GTAC_A
Cuts at: 96 3210 96
Size: 3114 3652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6766
BssSI C'ACGA_G
Cuts at: 4826 5838 4826
Size: 1012 5754
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3128 794
Size: 2334 4432
BstBI TT'CG_AA
Cuts at: 5570 5570
Size: 6766
BstEII G'GTnAC_C
Cuts at: 1184 2885 1184
Size: 1701 5065
BstYI r'GATC_y
Cuts at: 1972 2020 2137 2156 3496 4096 4463 4848 4865
Size: 48 117 19 1340 600 367 385 17
Cuts at: 4865 6317 6403 6415 6761 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1340 600 385 367 346 117
86 48 19 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 3636 513
Size: 552 939 30 1602 3643
Fragments arranged by size:
3643 1602 939 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6027
Fragments arranged by size:
6027 683 56
BtsI GCAGTG_nn'
Cuts at: 3931 4282 5076 5096 3931
Size: 351 794 20 5601
Fragments arranged by size:
5601 794 351 20
ClaI AT'CG_AT
Cuts at: 1554 2218 1554
Size: 664 6102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6731
DraI TTT'AAA
Cuts at: 2563 2950 3259 4070 2563
Size: 387 309 811 5259
Fragments arranged by size:
5259 811 387 309
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5773 809
Size: 507 4457 1802
Fragments arranged by size:
4457 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2466 4213
Size: 54 1425 50 275 18 498 138 1747
Cuts at: 4213 5102 6731 8
Size: 889 1629 43
Fragments arranged by size:
1747 1629 1425 889 498 275 138 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4213 1537
Size: 275 2401 4090
Fragments arranged by size:
4090 2401 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3987 4008 4696 1353
Size: 2634 21 688 3423
Fragments arranged by size:
3423 2634 688 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3557 5353 5727 5873 387
Size: 1064 138 1968 1796 374 146 1280
Fragments arranged by size:
1968 1796 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2584 2584
Size: 6766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 3251 3450 3458 3506 4050 4829
Size: 393 869 750 199 8 48 544 779
Cuts at: 4829 5426 6213 6481 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 869 787 779 750 597 544 393
268 199 48 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2822 2822
Size: 6766
EcoRI G'AATT_C
Cuts at: 4075 4453 4075
Size: 378 6388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2834 3336 2834
Size: 502 6264
FspI TGC'GCA
Cuts at: 5272 5272
Size: 6766
HaeII r_GCGC'y
Cuts at: 1678 5913 1678
Size: 4235 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2293 2325 2560 2592 2621
Size: 32 135 32 721 32 235 32 29
Cuts at: 2621 2653 3857 3889 3944 3976 5407 5439 5481
Size: 32 1204 32 55 32 1431 32 42
Cuts at: 5481 5513 1373
Size: 32 2626
Fragments arranged by size:
2626 1431 1204 721 235 135 55 42
32 32 32 32 32 32 32 32
32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2195 4110 4356 669
Size: 988 281 257 1915 246 3079
Fragments arranged by size:
3079 1915 988 281 257 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6766
HpaI GTT'AAC
Cuts at: 4356 4356
Size: 6766
KpnI G_GTAC'C
Cuts at: 3763 4091 3763
Size: 328 6438
MfeI C'AATT_G
Cuts at: 4365 4365
Size: 6766
MluI A'CGCG_T
Cuts at: 6642 6642
Size: 6766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 2577 2708 5880 6064 1305
Size: 942 330 131 3172 184 2007
Fragments arranged by size:
3172 2007 942 330 184 131
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 2468 10
Size: 54 1425 343 498 138 4308
Fragments arranged by size:
4308 1425 498 343 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2292 2407 3003 3009 3159 4724 5083
Size: 648 1126 115 596 6 150 1565 359
Cuts at: 5083 5242 518
Size: 159 2042
Fragments arranged by size:
2042 1565 1126 648 596 359 159 150
115 6
MspA1I CmG'CkG
Cuts at: 1606 3526 4182 4859 6007 6252 1606
Size: 1920 656 677 1148 245 2120
Fragments arranged by size:
2120 1920 1148 677 656 245
NaeI GCC'GGC
Cuts at: 3818 3818
Size: 6766
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5275
Fragments arranged by size:
5275 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6766
NgoMIV G'CCGG_C
Cuts at: 3816 3816
Size: 6766
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6766
NotI GC'GGCC_GC
Cuts at: 4213 4213
Size: 6766
NspI r_CATG'y
Cuts at: 1132 1229 4124 5669 1132
Size: 97 2895 1545 2229
Fragments arranged by size:
2895 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6766
PciI A'CATG_T
Cuts at: 5665 5665
Size: 6766
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2464 2789 3244 1108
Size: 1356 325 455 4630
Fragments arranged by size:
4630 1356 455 325
PfoI T'CCnGG_A
Cuts at: 2258 2258
Size: 6766
PmeI GTTT'AAAC
Cuts at: 4070 4070
Size: 6766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4835 4867 6348 6380 4835
Size: 32 1481 32 5221
Fragments arranged by size:
5221 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2203 2496 2719 1613
Size: 590 293 223 5660
Fragments arranged by size:
5660 590 293 223
PsiI TTA'TAA
Cuts at: 4336 4336
Size: 6766
PstI C_TGCA'G
Cuts at: 830 3043 3926 4118 830
Size: 2213 883 192 3478
Fragments arranged by size:
3478 2213 883 192
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6766
PvuII CAG'CTG
Cuts at: 3526 3526
Size: 6766
RsrII CG'GwC_CG
Cuts at: 3544 3544
Size: 6766
SacI G_AGCT'C
Cuts at: 2586 2586
Size: 6766
SalI G'TCGA_C
Cuts at: 1936 4108 1936
Size: 2172 4594
SbfI CC_TGCA'GG
Cuts at: 4118 4118
Size: 6766
ScaI AGT'ACT
Cuts at: 5014 6726 5014
Size: 1712 5054
SfcI C'TryA_G
Cuts at: 826 1045 2089 2519 3039 3922 4114 5249 5930
Size: 219 1044 430 520 883 192 1135 681
Cuts at: 5930 6121 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1044 883 681 520 430 219
192 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6766
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6766
SmaI CCC'GGG
Cuts at: 1872 4093 1872
Size: 2221 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3948 4870 5771 6033 820
Size: 197 926 2005 922 901 262 1553
Fragments arranged by size:
2005 1553 926 922 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4557 493
Size: 4064 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6766
SphI G_CATG'C
Cuts at: 1132 4124 1132
Size: 2992 3774
SspI AAT'ATT
Cuts at: 5 52 4690 5
Size: 47 4638 2081
Fragments arranged by size:
4638 2081 47
StuI AGG'CCT
Cuts at: 1517 2453 1517
Size: 936 5830
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 2783 2823 3571 4205 513
Size: 552 939 129 650 40 748 634 3074
Fragments arranged by size:
3074 939 748 650 634 552 129 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2419 2598 2603 3594 4793 4810 4963
Size: 1255 839 179 5 991 1199 17 153
Cuts at: 4963 5148 5593 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1199 991 839 445 185 179
153 17 5
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3210 3944 5012
Size: 274 80 33 51 1443 1233 734 1068
Cuts at: 5012 6724 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1233 1068 734 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 2607 2639 3617 3649 6726 6758 2022
Size: 32 553 32 978 32 3077 32 2030
Fragments arranged by size:
3077 2030 978 553 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2127 3704 1316
Size: 811 1577 4378
Fragments arranged by size:
4378 1577 811
XbaI T'CTAG_A
Cuts at: 4102 4102
Size: 6766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 6766
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6766
XmaI C'CCGG_G
Cuts at: 1870 4091 1870
Size: 2221 4545
XmnI GAAnn'nnTTC
Cuts at: 4895 4895
Size: 6766
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4574 276
Size: 53 83 186 936 3040 2468
Fragments arranged by size:
3040 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI