Restriction map for
pFN21AB6241
(Circular) (Six-base) MAPSORT of: pFN21AB6241.seq Check: 3635 from: 1 to: 6958
pFN21AB6241 6958 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4768 278
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3264 4301 1937
Size: 1327 1037 4594
Fragments arranged by size:
4594 1327 1037
Acc65I G'GTAC_C
Cuts at: 4279 4279
Size: 6958
AclI AA'CG_TT
Cuts at: 1563 3547 5085 5458 1563
Size: 1984 1538 373 3063
Fragments arranged by size:
3063 1984 1538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 2554 2722 3982 5021 6405 6673 1632
Size: 747 175 168 1260 1039 1384 268 1917
Fragments arranged by size:
1917 1384 1260 1039 747 268 175 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6761
AflIII A'CryG_T
Cuts at: 1203 2269 5857 6834 1203
Size: 1066 3588 977 1327
Fragments arranged by size:
3588 1327 1066 977
AgeI A'CCGG_T
Cuts at: 5789 5789
Size: 6958
AhdI GACnn_n'nnGTC
Cuts at: 5687 5687
Size: 6958
AleI CACnn'nnGTG
Cuts at: 1166 3537 4212 1166
Size: 2371 675 3912
Fragments arranged by size:
3912 2371 675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6958
AlwNI CAG_nnn'CTG
Cuts at: 4701 6273 4701
Size: 1572 5386
ApaI G_GGCC'C
Cuts at: 2110 2110
Size: 6958
ApaLI G'TGCA_C
Cuts at: 3014 5015 6171 3014
Size: 2001 1156 3801
Fragments arranged by size:
3801 2001 1156
ApoI r'AATT_y
Cuts at: 1493 2160 2524 4267 4493 4645 6754 1493
Size: 667 364 1743 226 152 2109 1697
Fragments arranged by size:
2109 1743 1697 667 364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5512 160
Size: 872 4480 1606
Fragments arranged by size:
4480 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
AvaI C'yCGr_G
Cuts at: 1870 1943 3703 4283 1870
Size: 73 1760 580 4545
Fragments arranged by size:
4545 1760 580 73
BamHI G'GATC_C
Cuts at: 4288 4655 4288
Size: 367 6591
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4279 5734 618
Size: 325 217 627 2492 1455 1842
Fragments arranged by size:
2492 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2110 4277 1391
Size: 705 14 2167 4072
Fragments arranged by size:
4072 2167 705 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2236 3785 928
Size: 971 337 1549 4101
Fragments arranged by size:
4101 1549 971 337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5149 5183 1903
Size: 34 3212 34 3678
Fragments arranged by size:
3678 3212 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4029 4850 6060 4029
Size: 821 1210 4927
Fragments arranged by size:
4927 1210 821
BclI T'GATC_A
Cuts at: 1548 2332 1548
Size: 784 6174
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2241 5569 136
Size: 107 122 71 631 605 569 3328 1525
Fragments arranged by size:
3328 1525 631 605 569 122 107 71
BglII A'GATC_T
Cuts at: 6953 6953
Size: 6958
BlpI GC'TnA_GC
Cuts at: 2090 4375 2090
Size: 2285 4673
Bme1580I G_kGCm'C
Cuts at: 1790 2110 2214 3018 3532 5019 6175 1790
Size: 320 104 804 514 1487 1156 2573
Fragments arranged by size:
2573 1487 1156 804 514 320 104
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6958
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4689 5647 442
Size: 416 53 83 399 24 3272 958 1753
Fragments arranged by size:
3272 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6958
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2115 2115
Size: 6958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 5618 1239
Size: 911 3468 2579
Fragments arranged by size:
3468 2579 911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2416 2729 5083 5948 6246 2416
Size: 313 2354 865 298 3128
Fragments arranged by size:
3128 2354 865 313 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5621 882
Size: 617 4122 2219
Fragments arranged by size:
4122 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4214 4749 493
Size: 711 3010 535 2702
Fragments arranged by size:
3010 2702 711 535
BsaBI GATnn'nnATC
Cuts at: 2588 6952 2588
Size: 4364 2594
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2233 4765 5147 5782
Size: 53 83 186 936 700 2532 382 635
Cuts at: 5782 275
Size: 1451
Fragments arranged by size:
2532 1451 936 700 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5391 5789 6063 6210 6621 5391
Size: 398 274 147 411 5728
Fragments arranged by size:
5728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2431 2461 4228 4258 1178
Size: 30 165 30 1028 30 1767 30 3878
Fragments arranged by size:
3878 1767 1028 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2452 2747 3167 3386 3470 3755 3923 1186
Size: 1266 295 420 219 84 285 168 4221
Fragments arranged by size:
4221 1266 420 295 285 219 168 84
BseYI C'CCAG_C
Cuts at: 6161 6657 6161
Size: 496 6462
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2271 2571 2737 3157 1163
Size: 573 535 300 166 420 4964
Fragments arranged by size:
4964 573 535 420 300 166
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4408 5169 6197 1540
Size: 275 186 2407 761 1028 2301
Fragments arranged by size:
2407 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3018 5019 5104 6175 3018
Size: 2001 85 1071 3801
Fragments arranged by size:
3801 2001 1071 85
BsmI GAATG_Cn'
Cuts at: 2540 2888 2944 4151 4469 4562 2540
Size: 348 56 1207 318 93 4936
Fragments arranged by size:
4936 1207 348 318 93 56
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2110 2214 3018 3532 4277 5019
Size: 399 306 14 104 804 514 745 742
Cuts at: 5019 5104 6175 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 804 745 742 514 399 306
104 85 14
BspEI T'CCGG_A
Cuts at: 6621 6621
Size: 6958
BspHI T'CATG_A
Cuts at: 4847 6577 4847
Size: 1730 5228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4313 844
Size: 3469 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4845 1394
Size: 429 3022 3507
Fragments arranged by size:
3507 3022 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2669 5453 5627 65
Size: 1183 1421 2784 174 1396
Fragments arranged by size:
2784 1421 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 5602 5789 1700
Size: 110 425 3367 187 2869
Fragments arranged by size:
3367 2869 425 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 4229 96
Size: 2016 2117 2825
Fragments arranged by size:
2825 2117 2016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6958
BssSI C'ACGA_G
Cuts at: 5018 6030 5018
Size: 1012 5946
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3732 794
Size: 2938 4020
BstBI TT'CG_AA
Cuts at: 5762 5762
Size: 6958
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6958
BstXI CCAn_nnnn'nTGG
Cuts at: 2894 3062 4157 2894
Size: 168 1095 5695
Fragments arranged by size:
5695 1095 168
BstYI r'GATC_y
Cuts at: 1972 4288 4655 5040 5057 6509 6595 6607 6953
Size: 2316 367 385 17 1452 86 12 346
Cuts at: 6953 1972
Size: 1977
Fragments arranged by size:
2316 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2013 3524 2013
Size: 1511 5447
BtgI C'CryG_G
Cuts at: 513 1065 2004 2391 513
Size: 552 939 387 5080
Fragments arranged by size:
5080 939 552 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6219
Fragments arranged by size:
6219 683 56
BtsI GCAGTG_nn'
Cuts at: 2725 3149 3653 4474 5268 5288 2725
Size: 424 504 821 794 20 4395
Fragments arranged by size:
4395 821 794 504 424 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6958
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2791 2826 2959 2994 3379 3414 3883
Size: 35 2227 35 133 35 385 35 469
Cuts at: 3883 3918 529
Size: 35 3569
Fragments arranged by size:
3569 2227 469 385 133 35 35 35
35 35
DraI TTT'AAA
Cuts at: 4262 4262
Size: 6958
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5965 809
Size: 507 4649 1802
Fragments arranged by size:
4649 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2237 3064 4405
Size: 54 1425 50 275 18 407 827 1341
Cuts at: 4405 5294 6923 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1341 889 827 407 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4405 1537
Size: 275 2593 4090
Fragments arranged by size:
4090 2593 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2217 3773 4001 4888 1353
Size: 864 1556 228 887 3423
Fragments arranged by size:
3423 1556 887 864 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4048 5545 5919 6065 387
Size: 1064 138 2459 1497 374 146 1280
Fragments arranged by size:
2459 1497 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2379 2554 2722 3982 5021 5618
Size: 393 518 229 175 168 1260 1039 597
Cuts at: 5618 6405 6673 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1260 1039 787 597 518 393 268
229 175 168
EcoO109I rG'GnC_Cy
Cuts at: 2009 2106 2107 2243 2009
Size: 97 1 136 6724
Fragments arranged by size:
6724 136 97 1
EcoRI G'AATT_C
Cuts at: 2160 4267 4645 2160
Size: 2107 378 4473
Fragments arranged by size:
4473 2107 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3022 3442 4225 3022
Size: 420 783 5755
Fragments arranged by size:
5755 783 420
FspI TGC'GCA
Cuts at: 5464 5464
Size: 6958
HaeII r_GCGC'y
Cuts at: 1678 6105 1678
Size: 4427 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2470 2502 2903 2935 3396
Size: 32 135 32 898 32 401 32 461
Cuts at: 3396 3428 3491 3523 3732 3764 4238 4270 5599
Size: 32 63 32 209 32 474 32 1329
Cuts at: 5599 5631 5673 5705 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 898 474 461 401 209 135
63 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3339 3423 4302 4548 669
Size: 988 281 1317 84 84 879 246 3079
Fragments arranged by size:
3079 1317 988 879 281 246 84 84
HindIII A'AGCT_T
Cuts at: 748 2338 3238 748
Size: 1590 900 4468
Fragments arranged by size:
4468 1590 900
HpaI GTT'AAC
Cuts at: 4548 4548
Size: 6958
KpnI G_GTAC'C
Cuts at: 4283 4283
Size: 6958
MfeI C'AATT_G
Cuts at: 4557 4557
Size: 6958
MluI A'CGCG_T
Cuts at: 6834 6834
Size: 6958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6072 6256 1305
Size: 4767 184 2007
Fragments arranged by size:
4767 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5136
Fragments arranged by size:
5136 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2545 3537 4212 4916 5275 5434 518
Size: 648 1379 992 675 704 359 159 2042
Fragments arranged by size:
2042 1379 992 704 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2357 4002 4374 5051 6199 6444 1606
Size: 751 1645 372 677 1148 245 2120
Fragments arranged by size:
2120 1645 1148 751 677 372 245
NaeI GCC'GGC
Cuts at: 2237 2237
Size: 6958
NcoI C'CATG_G
Cuts at: 513 1065 2004 2391 513
Size: 552 939 387 5080
Fragments arranged by size:
5080 939 552 387
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6958
NgoMIV G'CCGG_C
Cuts at: 2235 2235
Size: 6958
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6958
NotI GC'GGCC_GC
Cuts at: 4405 4405
Size: 6958
NspI r_CATG'y
Cuts at: 1132 1229 2273 2423 2634 4316 5861 1132
Size: 97 1044 150 211 1682 1545 2229
Fragments arranged by size:
2229 1682 1545 1044 211 150 97
PasI CC'CwG_GG
Cuts at: 1348 2214 1348
Size: 866 6092
PciI A'CATG_T
Cuts at: 2269 5857 2269
Size: 3588 3370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6958
PmeI GTTT'AAAC
Cuts at: 4262 4262
Size: 6958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5027 5059 6540 6572 5027
Size: 32 1481 32 5413
Fragments arranged by size:
5413 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2009 2009
Size: 6958
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6958
PsiI TTA'TAA
Cuts at: 4528 4528
Size: 6958
PspOMI G'GGCC_C
Cuts at: 2106 2106
Size: 6958
PstI C_TGCA'G
Cuts at: 830 3560 4310 830
Size: 2730 750 3478
Fragments arranged by size:
3478 2730 750
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6958
PvuII CAG'CTG
Cuts at: 2357 4002 2357
Size: 1645 5313
SalI G'TCGA_C
Cuts at: 1936 4300 1936
Size: 2364 4594
SbfI CC_TGCA'GG
Cuts at: 4310 4310
Size: 6958
ScaI AGT'ACT
Cuts at: 5206 6918 5206
Size: 1712 5246
SfcI C'TryA_G
Cuts at: 826 1045 2137 2490 3556 3808 4186 4306 5441
Size: 219 1092 353 1066 252 378 120 1135
Cuts at: 5441 6122 6313 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1092 1066 681 378 353 252
219 191 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6958
SmaI CCC'GGG
Cuts at: 1872 4285 1872
Size: 2413 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2431 2744 5062 5963 6225 820
Size: 197 926 488 313 2318 901 262 1553
Fragments arranged by size:
2318 1553 926 901 488 313 262 197
SnaBI TAC'GTA
Cuts at: 493 4749 493
Size: 4256 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6958
SphI G_CATG'C
Cuts at: 1132 4316 1132
Size: 3184 3774
SspI AAT'ATT
Cuts at: 5 52 4882 5
Size: 47 4830 2081
Fragments arranged by size:
4830 2081 47
StuI AGG'CCT
Cuts at: 1517 4138 1517
Size: 2621 4337
StyI C'CwwG_G
Cuts at: 513 1065 2004 2391 4397 513
Size: 552 939 387 2006 3074
Fragments arranged by size:
3074 2006 939 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 3527 4232 4985 5002 5155 5340
Size: 1255 504 1443 705 753 17 153 185
Cuts at: 5340 5785 325
Size: 445 1498
Fragments arranged by size:
1498 1443 1255 753 705 504 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 4229 5204
Size: 274 80 33 51 1443 135 2117 975
Cuts at: 5204 6916 96
Size: 1712 138
Fragments arranged by size:
2117 1712 1443 975 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2431 2463 4228 4260 6918 6950 2431
Size: 32 1765 32 2658 32 2439
Fragments arranged by size:
2658 2439 1765 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6958
XbaI T'CTAG_A
Cuts at: 4294 4294
Size: 6958
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6958
XmaI C'CCGG_G
Cuts at: 1870 4283 1870
Size: 2413 4545
XmnI GAAnn'nnTTC
Cuts at: 3632 3800 3968 5087 3632
Size: 168 168 1119 5503
Fragments arranged by size:
5503 1119 168 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4766 276
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI