Restriction map for
pFN21AB6240
(Circular) (Six-base) MAPSORT of: pFN21AB6240.seq Check: 5605 from: 1 to: 6736
pFN21AB6240 6736 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2512 3117 2512
Size: 605 6131
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4546 278
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 3748 4079 1937
Size: 1185 626 331 4594
Fragments arranged by size:
4594 1185 626 331
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 3588 4863 5236 1563
Size: 2025 1275 373 3063
Fragments arranged by size:
3063 2025 1275 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2198 2267 3960 4799 6183 6451 1632
Size: 542 24 69 1693 839 1384 268 1917
Fragments arranged by size:
1917 1693 1384 839 542 268 69 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 2774 3904 5635 6612 1203
Size: 1571 1130 1731 977 1327
Fragments arranged by size:
1731 1571 1327 1130 977
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6736
AlwNI CAG_nnn'CTG
Cuts at: 4479 6051 4479
Size: 1572 5164
ApaI G_GGCC'C
Cuts at: 3948 3948
Size: 6736
ApaLI G'TGCA_C
Cuts at: 4793 5949 4793
Size: 1156 5580
ApoI r'AATT_y
Cuts at: 1493 3374 3623 4045 4271 4423 6532 1493
Size: 1881 249 422 226 152 2109 1697
Fragments arranged by size:
2109 1881 1697 422 249 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5290 160
Size: 872 4258 1606
Fragments arranged by size:
4258 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 2630 4061 1870
Size: 73 687 1431 4545
Fragments arranged by size:
4545 1431 687 73
BamHI G'GATC_C
Cuts at: 4066 4433 4066
Size: 367 6369
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4057 5512 618
Size: 325 217 627 2270 1455 1842
Fragments arranged by size:
2270 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2372 3921 3948 4055 1391
Size: 981 1549 27 107 4072
Fragments arranged by size:
4072 1549 981 107 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2090 3551 928
Size: 971 191 1461 4113
Fragments arranged by size:
4113 1461 971 191
BbvCI CC'TCA_GC
Cuts at: 2373 3144 2373
Size: 771 5965
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4927 4961 1903
Size: 34 2990 34 3678
Fragments arranged by size:
3678 2990 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6736
BfrBI ATG'CAT
Cuts at: 3416 3416
Size: 6736
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5347 136
Size: 107 122 71 631 605 3675 1525
Fragments arranged by size:
3675 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3474 3875 6731 3474
Size: 401 2856 3479
Fragments arranged by size:
3479 2856 401
BlpI GC'TnA_GC
Cuts at: 2366 4153 2366
Size: 1787 4949
Bme1580I G_kGCm'C
Cuts at: 1790 3342 3948 4797 5953 1790
Size: 1552 606 849 1156 2573
Fragments arranged by size:
2573 1552 1156 849 606
BmgBI CAC'GTC
Cuts at: 1318 3907 1318
Size: 2589 4147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4467 5425 442
Size: 416 53 83 399 24 3050 958 1753
Fragments arranged by size:
3050 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3231 3231
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2366 2378 3432 5396 1239
Size: 1097 30 12 1054 1964 2579
Fragments arranged by size:
2579 1964 1097 1054 30 12
Bpu10I CC'TnA_GC
Cuts at: 2373 3144 2373
Size: 771 5965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2301 2649 4861 5726 6024 2301
Size: 348 2212 865 298 3013
Fragments arranged by size:
3013 2212 865 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5399 882
Size: 617 3900 2219
Fragments arranged by size:
3900 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 4527 493
Size: 711 2324 999 2702
Fragments arranged by size:
2702 2324 999 711
BsaBI GATnn'nnATC
Cuts at: 2248 6730 2248
Size: 4482 2254
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2126 4543 4925 5560
Size: 53 83 186 936 593 2417 382 635
Cuts at: 5560 275
Size: 1451
Fragments arranged by size:
2417 1451 936 635 593 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5169 5567 5841 5988 6399 5169
Size: 398 274 147 411 5506
Fragments arranged by size:
5506 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 5788
Fragments arranged by size:
5788 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 2773 2776 2900 1186
Size: 1195 392 3 124 5022
Fragments arranged by size:
5022 1195 392 124 3
BseYI C'CCAG_C
Cuts at: 2166 2331 2532 5939 6435 2166
Size: 165 201 3407 496 2467
Fragments arranged by size:
3407 2467 496 201 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2775 3685 1163
Size: 573 1039 910 4214
Fragments arranged by size:
4214 1039 910 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 4186 4947 5975 1540
Size: 275 186 132 2053 761 1028 2301
Fragments arranged by size:
2301 2053 1028 761 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 2719 2892 4797 4882 5953 2719
Size: 173 1905 85 1071 3502
Fragments arranged by size:
3502 1905 1071 173 85
BsmI GAATG_Cn'
Cuts at: 3541 4247 4340 3541
Size: 706 93 5937
Fragments arranged by size:
5937 706 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2117 2117
Size: 6736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2372 2719 2892 3342 3921 3948 4055
Size: 399 582 347 173 450 579 27 107
Cuts at: 4055 4797 4882 5953 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 582 579 450 399 347
173 107 85 27
BspEI T'CCGG_A
Cuts at: 6399 6399
Size: 6736
BspHI T'CATG_A
Cuts at: 4625 6355 4625
Size: 1730 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2512 3031 3117 4091 844
Size: 1668 519 86 974 3489
Fragments arranged by size:
3489 1668 974 519 86
BsrBI CCG'CTC
Cuts at: 1394 1823 4623 1394
Size: 429 2800 3507
Fragments arranged by size:
3507 2800 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3837 5231 5405 65
Size: 1183 2589 1394 174 1396
Fragments arranged by size:
2589 1396 1394 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2128 5380 5567 1700
Size: 110 318 3252 187 2869
Fragments arranged by size:
3252 2869 318 187 110
BsrGI T'GTAC_A
Cuts at: 96 3046 3901 96
Size: 2950 855 2931
Fragments arranged by size:
2950 2931 855
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 4796 5808 4796
Size: 1012 5724
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2362 3359 3892 794
Size: 1568 997 533 3638
Fragments arranged by size:
3638 1568 997 533
BstBI TT'CG_AA
Cuts at: 5540 5540
Size: 6736
BstEII G'GTnAC_C
Cuts at: 1184 3656 1184
Size: 2472 4264
BstYI r'GATC_y
Cuts at: 1972 2346 2761 3474 3875 4066 4433 4818 4835
Size: 374 415 713 401 191 367 385 17
Cuts at: 4835 6287 6373 6385 6731 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 713 415 401 385 374 367
346 191 86 17 12
BstZ17I GTA'TAC
Cuts at: 3749 3749
Size: 6736
BtgI C'CryG_G
Cuts at: 513 1065 2133 3771 513
Size: 552 1068 1638 3478
Fragments arranged by size:
3478 1638 1068 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5997
Fragments arranged by size:
5997 683 56
BtsI GCAGTG_nn'
Cuts at: 2955 3546 4252 5046 5066 2955
Size: 591 706 794 20 4625
Fragments arranged by size:
4625 794 706 591 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6701
DraI TTT'AAA
Cuts at: 4040 4040
Size: 6736
DraIII CAC_nnn'GTG
Cuts at: 3298 3298
Size: 6736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5743 809
Size: 507 4427 1802
Fragments arranged by size:
4427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2933 3522
Size: 54 1425 50 275 18 300 803 589
Cuts at: 3522 4183 5072 6701 8
Size: 661 889 1629 43
Fragments arranged by size:
1629 1425 889 803 661 589 300 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2130 4183 1537
Size: 275 318 2053 4090
Fragments arranged by size:
4090 2053 318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 3810 3909 4666 1353
Size: 933 1524 99 757 3423
Fragments arranged by size:
3423 1524 933 757 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5323 5697 5843 387
Size: 1064 138 3734 374 146 1280
Fragments arranged by size:
3734 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2198 2267 2336 2366 2378 3432
Size: 393 542 24 69 69 30 12 1054
Cuts at: 3432 3960 4799 5396 6183 6451 1239
Size: 528 839 597 787 268 1524
Fragments arranged by size:
1524 1054 839 787 597 542 528 393
268 69 69 30 24 12
EcoO109I rG'GnC_Cy
Cuts at: 2407 3263 3302 3944 3945 2407
Size: 856 39 642 1 5198
Fragments arranged by size:
5198 856 642 39 1
EcoRI G'AATT_C
Cuts at: 3623 4045 4423 3623
Size: 422 378 5936
Fragments arranged by size:
5936 422 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 6736
FspI TGC'GCA
Cuts at: 3363 5242 3363
Size: 1879 4857
HaeII r_GCGC'y
Cuts at: 1678 2608 5883 1678
Size: 930 3275 2531
Fragments arranged by size:
3275 2531 930
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2299 2331 2701
Size: 32 135 32 524 32 171 32 370
Cuts at: 2701 2733 2951 2983 5377 5409 5451 5483 1373
Size: 32 218 32 2394 32 42 32 2626
Fragments arranged by size:
2626 2394 524 370 218 171 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4080 4326 669
Size: 988 281 2142 246 3079
Fragments arranged by size:
3079 2142 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6736
HpaI GTT'AAC
Cuts at: 4326 4326
Size: 6736
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 4335 4335
Size: 6736
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 2702 3248 3535 5850 6034 1305
Size: 834 563 546 287 2315 184 2007
Fragments arranged by size:
2315 2007 834 563 546 287 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2935 10
Size: 54 1425 343 1103 3811
Fragments arranged by size:
3811 1425 1103 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3111 3287 3494 4694 5053 5212 518
Size: 648 1945 176 207 1200 359 159 2042
Fragments arranged by size:
2042 1945 1200 648 359 207 176 159
MspA1I CmG'CkG
Cuts at: 1606 2150 4152 4829 5977 6222 1606
Size: 544 2002 677 1148 245 2120
Fragments arranged by size:
2120 2002 1148 677 544 245
NaeI GCC'GGC
Cuts at: 2130 2130
Size: 6736
NcoI C'CATG_G
Cuts at: 513 1065 3771 513
Size: 552 2706 3478
Fragments arranged by size:
3478 2706 552
NdeI CA'TA_TG
Cuts at: 387 3412 387
Size: 3025 3711
NgoMIV G'CCGG_C
Cuts at: 2128 2128
Size: 6736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NsiI A_TGCA'T
Cuts at: 3418 3418
Size: 6736
NspI r_CATG'y
Cuts at: 1132 1229 2495 2778 2828 2999 3116 3292 3682
Size: 97 1266 283 50 171 117 176 390
Cuts at: 3682 4094 5639 1132
Size: 412 1545 2229
Fragments arranged by size:
2229 1545 1266 412 390 283 176 171
117 97 50
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6736
PciI A'CATG_T
Cuts at: 2774 5635 2774
Size: 2861 3875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2539 1108
Size: 1431 5305
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PmlI CAC'GTG
Cuts at: 3528 3528
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 6318 6350 4805
Size: 32 1481 32 5191
Fragments arranged by size:
5191 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3302 3302
Size: 6736
PshAI GACnn'nnGTC
Cuts at: 1613 2777 3251 3864 1613
Size: 1164 474 613 4485
Fragments arranged by size:
4485 1164 613 474
PsiI TTA'TAA
Cuts at: 2445 3381 4306 2445
Size: 936 925 4875
Fragments arranged by size:
4875 936 925
PspOMI G'GGCC_C
Cuts at: 3944 3944
Size: 6736
PstI C_TGCA'G
Cuts at: 830 2691 2728 4088 830
Size: 1861 37 1360 3478
Fragments arranged by size:
3478 1861 1360 37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2150 2150
Size: 6736
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SanDI GG'GwC_CC
Cuts at: 3302 3302
Size: 6736
SapI GCTCTTCn'nnn_
Cuts at: 2286 3810 3909 2286
Size: 1524 99 5113
Fragments arranged by size:
5113 1524 99
SbfI CC_TGCA'GG
Cuts at: 4088 4088
Size: 6736
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SfcI C'TryA_G
Cuts at: 826 1045 2687 2724 3062 3123 4084 5219 5900
Size: 219 1642 37 338 61 961 1135 681
Cuts at: 5900 6091 826
Size: 191 1471
Fragments arranged by size:
1642 1471 1135 961 681 338 219 191
61 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 2632 4063 1872
Size: 760 1431 4545
Fragments arranged by size:
4545 1431 760
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2316 2664 4840 5741 6003 820
Size: 197 926 373 348 2176 901 262 1553
Fragments arranged by size:
2176 1553 926 901 373 348 262 197
SnaBI TAC'GTA
Cuts at: 493 4527 493
Size: 4034 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6736
SphI G_CATG'C
Cuts at: 1132 2999 3292 4094 1132
Size: 1867 293 802 3774
Fragments arranged by size:
3774 1867 802 293
SrfI GCCC'GGGC
Cuts at: 2632 2632
Size: 6736
SspI AAT'ATT
Cuts at: 5 52 4660 5
Size: 47 4608 2081
Fragments arranged by size:
4608 2081 47
StuI AGG'CCT
Cuts at: 1517 2613 1517
Size: 1096 5640
StyI C'CwwG_G
Cuts at: 513 1065 2608 3259 3771 4175 513
Size: 552 1543 651 512 404 3074
Fragments arranged by size:
3074 1543 651 552 512 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3272 4763 4780 4933 5118 5563 325
Size: 1255 1692 1491 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1491 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3014 3031 3046
Size: 274 80 33 51 1443 1037 17 15
Cuts at: 3046 3901 4982 6694 96
Size: 855 1081 1712 138
Fragments arranged by size:
1712 1443 1081 1037 855 274 138 80
51 33 17 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6696 6728 6696
Size: 32 6704
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6736
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2909 2909
Size: 6736
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6736
XmaI C'CCGG_G
Cuts at: 1870 2630 4061 1870
Size: 760 1431 4545
Fragments arranged by size:
4545 1431 760
XmnI GAAnn'nnTTC
Cuts at: 4865 4865
Size: 6736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4544 276
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstXI
Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI
NruI PacI PfoI PspXI PsrI RsrII SacI SacII
SexAI SfiI SfoI SwaI