Restriction map for
pFN21AB6239
(Circular) (Six-base) MAPSORT of: pFN21AB6239.seq Check: 3634 from: 1 to: 6958
pFN21AB6239 6958 bp
With 182 enzymes: SgfI *
February 2, 2010 11:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4768 278
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3409 4301 1937
Size: 1472 892 4594
Fragments arranged by size:
4594 1472 892
Acc65I G'GTAC_C
Cuts at: 4279 4279
Size: 6958
AclI AA'CG_TT
Cuts at: 1563 5085 5458 1563
Size: 3522 373 3063
Fragments arranged by size:
3522 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2304 2604 3264 3345 4241 5021 6405 6673
Size: 672 300 660 81 896 780 1384 268
Cuts at: 6673 1632
Size: 1917
Fragments arranged by size:
1917 1384 896 780 672 660 300 268
81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6761
AflIII A'CryG_T
Cuts at: 1203 3404 4002 5857 6834 1203
Size: 2201 598 1855 977 1327
Fragments arranged by size:
2201 1855 1327 977 598
AgeI A'CCGG_T
Cuts at: 3507 5789 3507
Size: 2282 4676
AhdI GACnn_n'nnGTC
Cuts at: 5687 5687
Size: 6958
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6958
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2742 2774 2742
Size: 32 6926
AlwNI CAG_nnn'CTG
Cuts at: 3176 4701 6273 3176
Size: 1525 1572 3861
Fragments arranged by size:
3861 1572 1525
ApaLI G'TGCA_C
Cuts at: 2207 5015 6171 2207
Size: 2808 1156 2994
Fragments arranged by size:
2994 2808 1156
ApoI r'AATT_y
Cuts at: 1493 2148 2359 2511 2654 2670 3250 3640 3926
Size: 655 211 152 143 16 580 390 286
Cuts at: 3926 4068 4267 4493 4645 6754 1493
Size: 142 199 226 152 2109 1697
Fragments arranged by size:
2109 1697 655 580 390 286 226 211
199 152 152 143 142 16
AseI AT'TA_AT
Cuts at: 160 1032 3129 5512 160
Size: 872 2097 2383 1606
Fragments arranged by size:
2383 2097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
AvaI C'yCGr_G
Cuts at: 1870 1943 4283 1870
Size: 73 2340 4545
Fragments arranged by size:
4545 2340 73
BamHI G'GATC_C
Cuts at: 4288 4655 4288
Size: 367 6591
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3868 4279 5734 618
Size: 325 217 627 2081 411 1455 1842
Fragments arranged by size:
2081 1842 1455 627 411 325 217
BanII G_rGCy'C
Cuts at: 1391 2231 3239 3848 4277 1391
Size: 840 1008 609 429 4072
Fragments arranged by size:
4072 1008 840 609 429
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5987
BbvCI CC'TCA_GC
Cuts at: 3465 3465
Size: 6958
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4068 4102 5149 5183 1903
Size: 34 2131 34 1047 34 3678
Fragments arranged by size:
3678 2131 1047 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2190 3142 3469 4850 6060 2190
Size: 952 327 1381 1210 3088
Fragments arranged by size:
3088 1381 1210 952 327
BclI T'GATC_A
Cuts at: 1548 3589 4132 1548
Size: 2041 543 4374
Fragments arranged by size:
4374 2041 543
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5569 136
Size: 107 122 71 631 605 3897 1525
Fragments arranged by size:
3897 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2608 6953 2608
Size: 4345 2613
BlpI GC'TnA_GC
Cuts at: 3039 4375 3039
Size: 1336 5622
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2711 3623 3734 5019 6175 1790
Size: 421 500 912 111 1285 1156 2573
Fragments arranged by size:
2573 1285 1156 912 500 421 111
BmgBI CAC'GTC
Cuts at: 1318 3880 1318
Size: 2562 4396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 4689 5647
Size: 416 53 83 399 24 608 2664 958
Cuts at: 5647 442
Size: 1753
Fragments arranged by size:
2664 1753 958 608 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2633 3438 5618 1239
Size: 1394 805 2180 2579
Fragments arranged by size:
2579 2180 1394 805
Bpu10I CC'TnA_GC
Cuts at: 3465 3465
Size: 6958
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 3945 5083 5948 6246 2075
Size: 1870 1138 865 298 2787
Fragments arranged by size:
2787 1870 1138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2349 5621 882
Size: 617 850 3272 2219
Fragments arranged by size:
3272 2219 850 617
BsaAI yAC'GTr
Cuts at: 493 1204 4749 493
Size: 711 3545 2702
Fragments arranged by size:
3545 2702 711
BsaBI GATnn'nnATC
Cuts at: 3899 6952 3899
Size: 3053 3905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4765 5147 5782 275
Size: 53 83 186 936 3232 382 635 1451
Fragments arranged by size:
3232 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3507 5391 5789 6063 6210 6621 3507
Size: 1884 398 274 147 411 3844
Fragments arranged by size:
3844 1884 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2400 2430 2742 2772 1178
Size: 30 165 30 997 30 312 30 5364
Fragments arranged by size:
5364 997 312 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3229 3471 3547 1186
Size: 2043 242 76 4597
Fragments arranged by size:
4597 2043 242 76
BseYI C'CCAG_C
Cuts at: 3113 3888 6161 6657 3113
Size: 775 2273 496 3414
Fragments arranged by size:
3414 2273 775 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3504 3935 4027 1163
Size: 573 1768 431 92 4094
Fragments arranged by size:
4094 1768 573 431 92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4408 5169 6197 1540
Size: 275 186 2407 761 1028 2301
Fragments arranged by size:
2407 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2231 3239 5019 5104 6175 2211
Size: 20 1008 1780 85 1071 2994
Fragments arranged by size:
2994 1780 1071 1008 85 20
BsmI GAATG_Cn'
Cuts at: 2314 2536 4469 4562 2314
Size: 222 1933 93 4710
Fragments arranged by size:
4710 1933 222 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2568 2568
Size: 6958
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2231 2711 3239 3623 3734 3848
Size: 399 421 20 480 528 384 111 114
Cuts at: 3848 4277 5019 5104 6175 1391
Size: 429 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 528 480 429 421 399
384 114 111 85 20
BspEI T'CCGG_A
Cuts at: 6621 6621
Size: 6958
BspHI T'CATG_A
Cuts at: 4847 6577 4847
Size: 1730 5228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4313 844
Size: 3469 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3043 4845 1394
Size: 429 1220 1802 3507
Fragments arranged by size:
3507 1802 1220 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2231 3378 5453 5627 65
Size: 1183 983 1147 2075 174 1396
Fragments arranged by size:
2075 1396 1183 1147 983 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3507 5602 5789 1700
Size: 110 1697 2095 187 2869
Fragments arranged by size:
2869 2095 1697 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6958
BssSI C'ACGA_G
Cuts at: 5018 6030 5018
Size: 1012 5946
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3828 3862 794
Size: 3034 34 3890
Fragments arranged by size:
3890 3034 34
BstBI TT'CG_AA
Cuts at: 5762 5762
Size: 6958
BstEII G'GTnAC_C
Cuts at: 1184 2257 1184
Size: 1073 5885
BstXI CCAn_nnnn'nTGG
Cuts at: 2046 2269 2046
Size: 223 6735
BstYI r'GATC_y
Cuts at: 1972 2608 3907 4288 4655 5040 5057 6509 6595
Size: 636 1299 381 367 385 17 1452 86
Cuts at: 6595 6607 6953 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1299 636 385 381 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3596 513
Size: 552 939 1592 3875
Fragments arranged by size:
3875 1592 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2881 487
Size: 683 56 1655 4564
Fragments arranged by size:
4564 1655 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 3734 4474 5268 5288 3523
Size: 211 740 794 20 5193
Fragments arranged by size:
5193 794 740 211 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6958
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2959 2994 529
Size: 35 2395 35 4493
Fragments arranged by size:
4493 2395 35 35
DraI TTT'AAA
Cuts at: 2875 4262 2875
Size: 1387 5571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5965 809
Size: 507 4649 1802
Fragments arranged by size:
4649 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4405 5294 6923
Size: 54 1425 50 275 18 2575 889 1629
Cuts at: 6923 8
Size: 43
Fragments arranged by size:
2575 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4405 1537
Size: 275 2593 4090
Fragments arranged by size:
4090 2593 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3076 3207 3491 3973 4173 4888 1353
Size: 1723 131 284 482 200 715 3423
Fragments arranged by size:
3423 1723 715 482 284 200 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 5545 5919 6065 387
Size: 1064 138 476 3480 374 146 1280
Fragments arranged by size:
3480 1280 1064 476 374 146 138
EcoICRI GAG'CTC
Cuts at: 2229 3237 2229
Size: 1008 5950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 2604 2633 3264 3345 3438 4241
Size: 393 672 300 29 631 81 93 803
Cuts at: 4241 5021 5618 6405 6673 1239
Size: 780 597 787 268 1524
Fragments arranged by size:
1524 803 787 780 672 631 597 393
300 268 93 81 29
EcoRI G'AATT_C
Cuts at: 3926 4267 4645 3926
Size: 341 378 6239
Fragments arranged by size:
6239 378 341
FspI TGC'GCA
Cuts at: 5464 5464
Size: 6958
HaeII r_GCGC'y
Cuts at: 1678 6105 1678
Size: 4427 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3542 3574 3943 3975 5599
Size: 32 135 32 1970 32 369 32 1624
Cuts at: 5599 5631 5673 5705 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1970 1624 369 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4126 4302 4548 669
Size: 988 281 2188 176 246 3079
Fragments arranged by size:
3079 2188 988 281 246 176
HindIII A'AGCT_T
Cuts at: 748 2833 748
Size: 2085 4873
HpaI GTT'AAC
Cuts at: 4126 4548 4126
Size: 422 6536
KpnI G_GTAC'C
Cuts at: 4283 4283
Size: 6958
MfeI C'AATT_G
Cuts at: 4557 4557
Size: 6958
MluI A'CGCG_T
Cuts at: 6834 6834
Size: 6958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2303 6072 6256 1305
Size: 998 3769 184 2007
Fragments arranged by size:
3769 2007 998 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5136
Fragments arranged by size:
5136 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2625 3131 4916 5275 5434 518
Size: 648 1459 506 1785 359 159 2042
Fragments arranged by size:
2042 1785 1459 648 506 359 159
MspA1I CmG'CkG
Cuts at: 1606 3038 3464 3541 3702 3791 3841 4374 5051
Size: 1432 426 77 161 89 50 533 677
Cuts at: 5051 6199 6444 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1432 1148 677 533 426 245 161
89 77 50
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5467
Fragments arranged by size:
5467 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6958
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6958
NotI GC'GGCC_GC
Cuts at: 4405 4405
Size: 6958
NspI r_CATG'y
Cuts at: 1132 1229 2077 3408 4006 4316 5861 1132
Size: 97 848 1331 598 310 1545 2229
Fragments arranged by size:
2229 1545 1331 848 598 310 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6958
PciI A'CATG_T
Cuts at: 3404 4002 5857 3404
Size: 598 1855 4505
Fragments arranged by size:
4505 1855 598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3001 1108
Size: 1893 5065
PfoI T'CCnGG_A
Cuts at: 3067 3691 3067
Size: 624 6334
PmeI GTTT'AAAC
Cuts at: 4262 4262
Size: 6958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2742 2774 5027 5059 6540 6572 2742
Size: 32 2253 32 1481 32 3128
Fragments arranged by size:
3128 2253 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3771 1613
Size: 2158 4800
PsiI TTA'TAA
Cuts at: 2501 2937 4528 2501
Size: 436 1591 4931
Fragments arranged by size:
4931 1591 436
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2116 2148 2116
Size: 32 6926
PstI C_TGCA'G
Cuts at: 830 3530 3833 4310 830
Size: 2700 303 477 3478
Fragments arranged by size:
3478 2700 477 303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6958
PvuII CAG'CTG
Cuts at: 3541 3541
Size: 6958
SacI G_AGCT'C
Cuts at: 2231 3239 2231
Size: 1008 5950
SalI G'TCGA_C
Cuts at: 1936 4300 1936
Size: 2364 4594
SapI GCTCTTCn'nnn_
Cuts at: 4173 4173
Size: 6958
SbfI CC_TGCA'GG
Cuts at: 4310 4310
Size: 6958
ScaI AGT'ACT
Cuts at: 5206 6918 5206
Size: 1712 5246
SfcI C'TryA_G
Cuts at: 826 1045 2351 2860 3526 3829 4306 5441 6122
Size: 219 1306 509 666 303 477 1135 681
Cuts at: 6122 6313 826
Size: 191 1471
Fragments arranged by size:
1471 1306 1135 681 666 509 477 303
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6958
SmaI CCC'GGG
Cuts at: 1872 4285 1872
Size: 2413 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 3960 5062 5963 6225 820
Size: 197 926 147 1870 1102 901 262 1553
Fragments arranged by size:
1870 1553 1102 926 901 262 197 147
SnaBI TAC'GTA
Cuts at: 493 4749 493
Size: 4256 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6958
SphI G_CATG'C
Cuts at: 1132 4316 1132
Size: 3184 3774
SspI AAT'ATT
Cuts at: 5 52 3289 4882 5
Size: 47 3237 1593 2081
Fragments arranged by size:
3237 2081 1593 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6958
StyI C'CwwG_G
Cuts at: 513 1065 2004 2536 3485 4397 513
Size: 552 939 532 949 912 3074
Fragments arranged by size:
3074 949 939 912 552 532
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2941 4985 5002 5155 5340 5785 325
Size: 1255 1361 2044 17 153 185 445 1498
Fragments arranged by size:
2044 1498 1361 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2070 2473 5204
Size: 274 80 33 51 1443 93 403 2731
Cuts at: 5204 6916 96
Size: 1712 138
Fragments arranged by size:
2731 1712 1443 403 274 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 6918 6950 2274
Size: 32 4612 32 2282
Fragments arranged by size:
4612 2282 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6958
XbaI T'CTAG_A
Cuts at: 2962 4294 2962
Size: 1332 5626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3133 3133
Size: 6958
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6958
XmaI C'CCGG_G
Cuts at: 1870 4283 1870
Size: 2413 4545
XmnI GAAnn'nnTTC
Cuts at: 5087 5087
Size: 6958
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4766 276
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI