Restriction map for
pFN21AB6237
(Circular) (Six-base) MAPSORT of: pFN21AB6237.seq Check: 9442 from: 1 to: 6958
pFN21AB6237 6958 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4768 278
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4301 1937
Size: 2364 4594
Acc65I G'GTAC_C
Cuts at: 2291 4279 2291
Size: 1988 4970
AclI AA'CG_TT
Cuts at: 1563 5085 5458 1563
Size: 3522 373 3063
Fragments arranged by size:
3522 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2376 2764 3268 3436 3688 4192 5021 6405
Size: 744 388 504 168 252 504 829 1384
Cuts at: 6405 6673 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 829 744 504 504 388 268
252 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6761
AflIII A'CryG_T
Cuts at: 1203 5857 6834 1203
Size: 4654 977 1327
Fragments arranged by size:
4654 1327 977
AgeI A'CCGG_T
Cuts at: 5789 5789
Size: 6958
AhdI GACnn_n'nnGTC
Cuts at: 5687 5687
Size: 6958
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6958
AlwNI CAG_nnn'CTG
Cuts at: 2345 2482 3459 4243 4701 6273 2345
Size: 137 977 784 458 1572 3030
Fragments arranged by size:
3030 1572 977 784 458 137
ApaI G_GGCC'C
Cuts at: 3210 3210
Size: 6958
ApaLI G'TGCA_C
Cuts at: 5015 6171 5015
Size: 1156 5802
ApoI r'AATT_y
Cuts at: 1493 2549 2638 2814 2898 2982 3066 3150 3402
Size: 1056 89 176 84 84 84 84 252
Cuts at: 3402 3486 3654 3822 3990 4158 4267 4493 4645
Size: 84 168 168 168 168 109 226 152
Cuts at: 4645 6754 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1056 252 226 176 168 168
168 168 152 109 89 84 84 84
84 84
AseI AT'TA_AT
Cuts at: 160 1032 5512 160
Size: 872 4480 1606
Fragments arranged by size:
4480 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
AvaI C'yCGr_G
Cuts at: 1870 1943 4283 1870
Size: 73 2340 4545
Fragments arranged by size:
4545 2340 73
BamHI G'GATC_C
Cuts at: 4288 4655 4288
Size: 367 6591
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2291 4279 5734 618
Size: 325 217 627 504 1988 1455 1842
Fragments arranged by size:
1988 1842 1455 627 504 325 217
BanII G_rGCy'C
Cuts at: 1391 3210 3968 4277 1391
Size: 1819 758 309 4072
Fragments arranged by size:
4072 1819 758 309
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5987
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5149 5183 1903
Size: 34 3212 34 3678
Fragments arranged by size:
3678 3212 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4850 6060 4850
Size: 1210 5748
BclI T'GATC_A
Cuts at: 1548 2845 1548
Size: 1297 5661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3042 5569 136
Size: 107 122 71 631 605 1370 2527 1525
Fragments arranged by size:
2527 1525 1370 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2011 6953 2011
Size: 4942 2016
BlpI GC'TnA_GC
Cuts at: 3642 4375 3642
Size: 733 6225
Bme1580I G_kGCm'C
Cuts at: 1790 3210 5019 6175 1790
Size: 1420 1809 1156 2573
Fragments arranged by size:
2573 1809 1420 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6958
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2233 2314 2514
Size: 416 53 83 399 24 816 81 200
Cuts at: 2514 2642 3336 4008 4689 5647 442
Size: 128 694 672 681 958 1753
Fragments arranged by size:
1753 958 816 694 681 672 416 399
200 128 83 81 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6958
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3651 3651
Size: 6958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2007 2355 2586 2760 2844 2928 3180 3432
Size: 768 348 231 174 84 84 252 252
Cuts at: 3432 3684 3768 4104 5618 1239
Size: 252 84 336 1514 2579
Fragments arranged by size:
2579 1514 768 348 336 252 252 252
231 174 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3564 5083 5948 6246 3564
Size: 1519 865 298 4276
Fragments arranged by size:
4276 1519 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2203 2603 5621 882
Size: 617 704 400 3018 2219
Fragments arranged by size:
3018 2219 704 617 400
BsaAI yAC'GTr
Cuts at: 493 1204 4749 493
Size: 711 3545 2702
Fragments arranged by size:
3545 2702 711
BsaBI GATnn'nnATC
Cuts at: 6952 6952
Size: 6958
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4765 5147 5782 275
Size: 53 83 186 936 3232 382 635 1451
Fragments arranged by size:
3232 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5391 5789 6063 6210 6621 5391
Size: 398 274 147 411 5728
Fragments arranged by size:
5728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6733
Fragments arranged by size:
6733 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6958
BseYI C'CCAG_C
Cuts at: 6161 6657 6161
Size: 496 6462
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2319 3369 1163
Size: 573 583 1050 4752
Fragments arranged by size:
4752 1050 583 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4408 5169 6197 1540
Size: 275 186 2407 761 1028 2301
Fragments arranged by size:
2407 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3128 3968 4052 4136 5019 5104 6175 3128
Size: 840 84 84 883 85 1071 3911
Fragments arranged by size:
3911 1071 883 840 85 84 84
BsiWI C'GTAC_G
Cuts at: 3255 3255
Size: 6958
BsmI GAATG_Cn'
Cuts at: 2280 3266 3518 3785 4115 4469 4562 2280
Size: 986 252 267 330 354 93 4676
Fragments arranged by size:
4676 986 354 330 267 252 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3128 3210 3968 4052 4136 4277 5019
Size: 399 1338 82 758 84 84 141 742
Cuts at: 5019 5104 6175 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1338 1071 758 742 399 141 85
84 84 82
BspEI T'CCGG_A
Cuts at: 6621 6621
Size: 6958
BspHI T'CATG_A
Cuts at: 3685 4847 6577 3685
Size: 1162 1730 4066
Fragments arranged by size:
4066 1730 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4313 844
Size: 3469 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4845 1394
Size: 429 3022 3507
Fragments arranged by size:
3507 3022 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2683 3271 3439 4027 5453 5627 65
Size: 1183 1435 588 168 588 1426 174 1396
Fragments arranged by size:
1435 1426 1396 1183 588 588 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 5602 5789 1700
Size: 110 3792 187 2869
Fragments arranged by size:
3792 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6958
BssSI C'ACGA_G
Cuts at: 3493 5018 6030 3493
Size: 1525 1012 4421
Fragments arranged by size:
4421 1525 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6958
BstBI TT'CG_AA
Cuts at: 5762 5762
Size: 6958
BstEII G'GTnAC_C
Cuts at: 1184 2500 1184
Size: 1316 5642
BstYI r'GATC_y
Cuts at: 1972 2011 3738 4288 4655 5040 5057 6509 6595
Size: 39 1727 550 367 385 17 1452 86
Cuts at: 6595 6607 6953 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1727 1452 550 385 367 346 86
39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2261 513
Size: 552 939 257 5210
Fragments arranged by size:
5210 939 552 257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6219
Fragments arranged by size:
6219 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 3523 4474 5268 5288 2480
Size: 1043 951 794 20 4150
Fragments arranged by size:
4150 1043 951 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6958
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2665 2700 3253 3288 3421 3456 529
Size: 35 2101 35 553 35 133 35 4031
Fragments arranged by size:
4031 2101 553 133 35 35 35 35
DraI TTT'AAA
Cuts at: 3091 4262 3091
Size: 1171 5787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5965 809
Size: 507 4649 1802
Fragments arranged by size:
4649 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2146 4405 5294
Size: 54 1425 50 275 18 316 2259 889
Cuts at: 5294 6923 8
Size: 1629 43
Fragments arranged by size:
2259 1629 1425 889 316 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4405 1537
Size: 275 2593 4090
Fragments arranged by size:
4090 2593 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2355 2601 4888 1353
Size: 1002 246 2287 3423
Fragments arranged by size:
3423 2287 1002 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5545 5919 6065 387
Size: 1064 138 3956 374 146 1280
Fragments arranged by size:
3956 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3966 3966
Size: 6958
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2355 2376 2586 2760 2764 2844
Size: 393 375 348 21 210 174 4 80
Cuts at: 2844 2928 3180 3268 3432 3436 3684 3688 3768
Size: 84 252 88 164 4 248 4 80
Cuts at: 3768 4104 4192 5021 5618 6405 6673 1239
Size: 336 88 829 597 787 268 1524
Fragments arranged by size:
1524 829 787 597 393 375 348 336
268 252 248 210 174 164 88 88
84 80 80 21 4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3118 3118
Size: 6958
EcoO109I rG'GnC_Cy
Cuts at: 2534 2958 3207 2534
Size: 424 249 6285
Fragments arranged by size:
6285 424 249
EcoRI G'AATT_C
Cuts at: 2638 2814 2898 2982 3486 3654 3822 3990 4158
Size: 176 84 84 504 168 168 168 168
Cuts at: 4158 4267 4645 2638
Size: 109 378 4951
Fragments arranged by size:
4951 504 378 176 168 168 168 168
109 84 84
EcoRV GAT'ATC
Cuts at: 4214 4214
Size: 6958
FspI TGC'GCA
Cuts at: 5464 5464
Size: 6958
HaeII r_GCGC'y
Cuts at: 1678 6105 1678
Size: 4427 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2861 2893 2947
Size: 32 135 32 431 32 826 32 54
Cuts at: 2947 2979 3955 3987 4039 4051 4071 4083 4123
Size: 32 976 32 52 12 20 12 40
Cuts at: 4123 4155 4231 4263 5599 5631 5673 5705 1373
Size: 32 76 32 1336 32 42 32 2626
Fragments arranged by size:
2626 1336 976 826 431 135 76 54
52 42 40 32 32 32 32 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4302 4548 669
Size: 988 281 2364 246 3079
Fragments arranged by size:
3079 2364 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3588 3615 748
Size: 2840 27 4091
Fragments arranged by size:
4091 2840 27
HpaI GTT'AAC
Cuts at: 4548 4548
Size: 6958
KpnI G_GTAC'C
Cuts at: 2295 4283 2295
Size: 1988 4970
MfeI C'AATT_G
Cuts at: 4557 4557
Size: 6958
MluI A'CGCG_T
Cuts at: 6834 6834
Size: 6958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2442 6072 6256 1305
Size: 1137 3630 184 2007
Fragments arranged by size:
3630 2007 1137 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2148 10
Size: 54 1425 343 316 4820
Fragments arranged by size:
4820 1425 343 316 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2839 2923 3243 3690 4916 5275 5434
Size: 648 1673 84 320 447 1226 359 159
Cuts at: 5434 518
Size: 2042
Fragments arranged by size:
2042 1673 1226 648 447 359 320 159
84
MspA1I CmG'CkG
Cuts at: 1606 2137 4374 5051 6199 6444 1606
Size: 531 2237 677 1148 245 2120
Fragments arranged by size:
2237 2120 1148 677 531 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2261 513
Size: 552 939 257 5210
Fragments arranged by size:
5210 939 552 257
NdeI CA'TA_TG
Cuts at: 387 3231 387
Size: 2844 4114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6958
NotI GC'GGCC_GC
Cuts at: 4405 4405
Size: 6958
NspI r_CATG'y
Cuts at: 1132 1229 4316 5861 1132
Size: 97 3087 1545 2229
Fragments arranged by size:
3087 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6958
PciI A'CATG_T
Cuts at: 5857 5857
Size: 6958
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3052 1108
Size: 1944 5014
PmeI GTTT'AAAC
Cuts at: 4262 4262
Size: 6958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5027 5059 6540 6572 5027
Size: 32 1481 32 5413
Fragments arranged by size:
5413 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2958 2958
Size: 6958
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6958
PsiI TTA'TAA
Cuts at: 2801 4098 4528 2801
Size: 1297 430 5231
Fragments arranged by size:
5231 1297 430
PspOMI G'GGCC_C
Cuts at: 3206 3206
Size: 6958
PstI C_TGCA'G
Cuts at: 830 4310 830
Size: 3480 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6958
PvuII CAG'CTG
Cuts at: 2137 2137
Size: 6958
SacI G_AGCT'C
Cuts at: 3968 3968
Size: 6958
SalI G'TCGA_C
Cuts at: 1936 4300 1936
Size: 2364 4594
SapI GCTCTTCn'nnn_
Cuts at: 2355 2355
Size: 6958
SbfI CC_TGCA'GG
Cuts at: 4310 4310
Size: 6958
ScaI AGT'ACT
Cuts at: 5206 6918 5206
Size: 1712 5246
SexAI A'CCwGG_T
Cuts at: 2193 2193
Size: 6958
SfcI C'TryA_G
Cuts at: 826 1045 2202 4306 5441 6122 6313 826
Size: 219 1157 2104 1135 681 191 1471
Fragments arranged by size:
2104 1471 1157 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6958
SmaI CCC'GGG
Cuts at: 1872 4285 1872
Size: 2413 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3579 5062 5963 6225 820
Size: 197 926 1636 1483 901 262 1553
Fragments arranged by size:
1636 1553 1483 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4749 493
Size: 4256 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6958
SphI G_CATG'C
Cuts at: 1132 4316 1132
Size: 3184 3774
SspI AAT'ATT
Cuts at: 5 52 4882 5
Size: 47 4830 2081
Fragments arranged by size:
4830 2081 47
StuI AGG'CCT
Cuts at: 1517 3197 3449 1517
Size: 1680 252 5026
Fragments arranged by size:
5026 1680 252
StyI C'CwwG_G
Cuts at: 513 1065 2004 2041 2261 4397 513
Size: 552 939 37 220 2136 3074
Fragments arranged by size:
3074 2136 939 552 220 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4985 5002 5155 5340 5785 325
Size: 1255 3405 17 153 185 445 1498
Fragments arranged by size:
3405 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5204 6916 96
Size: 274 80 33 51 1443 3227 1712 138
Fragments arranged by size:
3227 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6918 6950 6918
Size: 32 6926
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6958
XbaI T'CTAG_A
Cuts at: 4294 4294
Size: 6958
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6958
XmaI C'CCGG_G
Cuts at: 1870 4283 1870
Size: 2413 4545
XmnI GAAnn'nnTTC
Cuts at: 3281 3654 3701 4162 5087 3281
Size: 373 47 461 925 5152
Fragments arranged by size:
5152 925 461 373 47
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4766 276
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI XcmI