(Circular) (Six-base) MAPSORT of: pFN21AB6235.seq Check: 3990 from: 1 to: 6952 pFN21AB6235 6952 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2080 2080 Size: 6952 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3345 4762 278 Size: 53 83 186 936 1809 1417 2468 Fragments arranged by size: 2468 1809 1417 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3229 3591 3886 4295 1937 Size: 1292 362 295 409 4594 Fragments arranged by size: 4594 1292 409 362 295 Acc65I G'GTAC_C Cuts at: 4273 4273 Size: 6952 AclI AA'CG_TT Cuts at: 1563 5079 5452 1563 Size: 3516 373 3063 Fragments arranged by size: 3516 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3527 3668 4050 5015 6399 6667 1632 Size: 1895 141 382 965 1384 268 1917 Fragments arranged by size: 1917 1895 1384 965 382 268 141 AfeI AGC'GCT Cuts at: 4225 4225 Size: 6952 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6755 AflIII A'CryG_T Cuts at: 1203 2155 3501 5851 6828 1203 Size: 952 1346 2350 977 1327 Fragments arranged by size: 2350 1346 1327 977 952 AgeI A'CCGG_T Cuts at: 5783 5783 Size: 6952 AhdI GACnn_n'nnGTC Cuts at: 2518 5681 2518 Size: 3163 3789 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3729 1184 Size: 2545 4407 AlwNI CAG_nnn'CTG Cuts at: 2021 2563 3490 3796 4695 6267 2021 Size: 542 927 306 899 1572 2706 Fragments arranged by size: 2706 1572 927 899 542 306 ApaLI G'TGCA_C Cuts at: 5009 6165 5009 Size: 1156 5796 ApoI r'AATT_y Cuts at: 1493 2431 3385 4261 4487 4639 6748 1493 Size: 938 954 876 226 152 2109 1697 Fragments arranged by size: 2109 1697 954 938 876 226 152 AseI AT'TA_AT Cuts at: 160 1032 2687 5506 160 Size: 872 1655 2819 1606 Fragments arranged by size: 2819 1655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 AvaI C'yCGr_G Cuts at: 1870 1943 2460 2985 4139 4277 1870 Size: 73 517 525 1154 138 4545 Fragments arranged by size: 4545 1154 525 517 138 73 BamHI G'GATC_C Cuts at: 4282 4649 4282 Size: 367 6585 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4273 5728 618 Size: 325 217 627 2486 1455 1842 Fragments arranged by size: 2486 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4271 1391 Size: 2880 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2811 3769 928 Size: 971 912 958 4111 Fragments arranged by size: 4111 971 958 912 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5143 5177 1903 Size: 34 50 34 3122 34 3678 Fragments arranged by size: 3678 3122 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2244 4844 6054 2244 Size: 2600 1210 3142 Fragments arranged by size: 3142 2600 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6952 BfrBI ATG'CAT Cuts at: 2981 2981 Size: 6952 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5563 136 Size: 107 122 71 631 605 3891 1525 Fragments arranged by size: 3891 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6947 6947 Size: 6952 BlpI GC'TnA_GC Cuts at: 4100 4369 4100 Size: 269 6683 Bme1580I G_kGCm'C Cuts at: 1790 2348 2732 5013 6169 1790 Size: 558 384 2281 1156 2573 Fragments arranged by size: 2573 2281 1156 558 384 BmgBI CAC'GTC Cuts at: 1318 3504 1318 Size: 2186 4766 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2031 2654 3762 Size: 416 53 83 399 24 614 623 1108 Cuts at: 3762 4683 5641 442 Size: 921 958 1753 Fragments arranged by size: 1753 1108 958 921 623 614 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3861 1056 Size: 2805 4147 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2399 2399 Size: 6952 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 2418 2540 3722 5612 1239 Size: 1053 126 122 1182 1890 2579 Fragments arranged by size: 2579 1890 1182 1053 126 122 Bpu10I CC'TnA_GC Cuts at: 2169 3933 2169 Size: 1764 5188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2277 4059 5077 5942 6240 2277 Size: 1782 1018 865 298 2989 Fragments arranged by size: 2989 1782 1018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2329 5615 882 Size: 617 830 3286 2219 Fragments arranged by size: 3286 2219 830 617 BsaAI yAC'GTr Cuts at: 493 1204 4743 493 Size: 711 3539 2702 Fragments arranged by size: 3539 2702 711 BsaBI GATnn'nnATC Cuts at: 3332 6946 3332 Size: 3614 3338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3342 4759 5141 Size: 53 83 186 936 834 975 1417 382 Cuts at: 5141 5776 275 Size: 635 1451 Fragments arranged by size: 1451 1417 975 936 834 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5385 5783 6057 6204 6615 5385 Size: 398 274 147 411 5722 Fragments arranged by size: 5722 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4146 4176 1178 Size: 30 165 30 2743 30 3954 Fragments arranged by size: 3954 2743 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6952 BseYI C'CCAG_C Cuts at: 2470 6155 6651 2470 Size: 3685 496 2771 Fragments arranged by size: 3685 2771 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 2195 3555 4240 1163 Size: 573 426 33 1360 685 3875 Fragments arranged by size: 3875 1360 685 573 426 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4402 5163 6191 1540 Size: 275 186 2401 761 1028 2301 Fragments arranged by size: 2401 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2992 3084 3698 4188 5013 5098 6169 2992 Size: 92 614 490 825 85 1071 3775 Fragments arranged by size: 3775 1071 825 614 490 92 85 BsmI GAATG_Cn' Cuts at: 4463 4556 4463 Size: 93 6859 BsmBI CGTCTCn'nnnn_ Cuts at: 2489 2489 Size: 6952 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2348 2732 2992 3084 3698 4188 4271 Size: 399 558 384 260 92 614 490 83 Cuts at: 4271 5013 5098 6169 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 614 558 490 399 384 260 92 85 83 BspEI T'CCGG_A Cuts at: 6615 6615 Size: 6952 BspHI T'CATG_A Cuts at: 4841 6571 4841 Size: 1730 5222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2080 2119 2137 4307 844 Size: 1236 39 18 2170 3489 Fragments arranged by size: 3489 2170 1236 39 18 BsrBI CCG'CTC Cuts at: 1394 1823 2168 4839 1394 Size: 429 345 2671 3507 Fragments arranged by size: 3507 2671 429 345 BsrDI GCAATG_nn' Cuts at: 65 1248 5447 5621 65 Size: 1183 4199 174 1396 Fragments arranged by size: 4199 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5596 5783 1700 Size: 110 3786 187 2869 Fragments arranged by size: 3786 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2256 2616 96 Size: 2160 360 4432 Fragments arranged by size: 4432 2160 360 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6952 BssSI C'ACGA_G Cuts at: 3531 5012 6024 3531 Size: 1481 1012 4459 Fragments arranged by size: 4459 1481 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3533 3796 794 Size: 2739 263 3950 Fragments arranged by size: 3950 2739 263 BstBI TT'CG_AA Cuts at: 3382 5756 3382 Size: 2374 4578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6952 BstXI CCAn_nnnn'nTGG Cuts at: 2270 2725 2759 2270 Size: 455 34 6463 Fragments arranged by size: 6463 455 34 BstYI r'GATC_y Cuts at: 1972 4282 4649 5034 5051 6503 6589 6601 6947 Size: 2310 367 385 17 1452 86 12 346 Cuts at: 6947 1972 Size: 1977 Fragments arranged by size: 2310 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2319 3301 513 Size: 552 1254 982 4164 Fragments arranged by size: 4164 1254 982 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6213 Fragments arranged by size: 6213 683 56 BtsI GCAGTG_nn' Cuts at: 2085 3059 3543 3713 3856 4030 4242 4468 5262 Size: 974 484 170 143 174 212 226 794 Cuts at: 5262 5282 2085 Size: 20 3755 Fragments arranged by size: 3755 974 794 484 226 212 174 170 143 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6917 DraI TTT'AAA Cuts at: 3984 4256 3984 Size: 272 6680 DraIII CAC_nnn'GTG Cuts at: 2446 2446 Size: 6952 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5959 809 Size: 507 4643 1802 Fragments arranged by size: 4643 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4399 5288 6917 Size: 54 1425 50 275 18 2569 889 1629 Cuts at: 6917 8 Size: 43 Fragments arranged by size: 2569 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4399 1537 Size: 275 2587 4090 Fragments arranged by size: 4090 2587 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2544 3025 3687 4069 4882 1353 Size: 1191 481 662 382 813 3423 Fragments arranged by size: 3423 1191 813 662 481 382 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2293 5539 5913 6059 387 Size: 1064 138 704 3246 374 146 1280 Fragments arranged by size: 3246 1280 1064 704 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2418 2540 3527 3668 3722 4050 Size: 393 660 126 122 987 141 54 328 Cuts at: 4050 5015 5612 6399 6667 1239 Size: 965 597 787 268 1524 Fragments arranged by size: 1524 987 965 787 660 597 393 328 268 141 126 122 54 EcoNI CCTnn'n_nnAGG Cuts at: 3521 3521 Size: 6952 EcoRI G'AATT_C Cuts at: 2431 4261 4639 2431 Size: 1830 378 4744 Fragments arranged by size: 4744 1830 378 EcoRV GAT'ATC Cuts at: 3052 3052 Size: 6952 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3428 3428 Size: 6952 FspI TGC'GCA Cuts at: 5458 5458 Size: 6952 HaeII r_GCGC'y Cuts at: 1678 3160 4227 6099 1678 Size: 1482 1067 1872 2531 Fragments arranged by size: 2531 1872 1482 1067 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2021 2041 2053 2370 Size: 32 135 32 437 12 20 12 317 Cuts at: 2370 2402 2504 2536 3041 3073 5593 5625 5667 Size: 32 102 32 505 32 2520 32 42 Cuts at: 5667 5699 1373 Size: 32 2626 Fragments arranged by size: 2626 2520 505 437 317 135 102 42 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2912 3230 3592 4296 4542 669 Size: 988 281 974 318 362 704 246 3079 Fragments arranged by size: 3079 988 974 704 362 318 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6952 HpaI GTT'AAC Cuts at: 4542 4542 Size: 6952 KpnI G_GTAC'C Cuts at: 4277 4277 Size: 6952 MfeI C'AATT_G Cuts at: 2478 4551 2478 Size: 2073 4879 MluI A'CGCG_T Cuts at: 6828 6828 Size: 6952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 2547 3200 6066 6250 1305 Size: 702 540 653 2866 184 2007 Fragments arranged by size: 2866 2007 702 653 540 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5130 Fragments arranged by size: 5130 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2757 4910 5269 5428 518 Size: 648 1591 2153 359 159 2042 Fragments arranged by size: 2153 2042 1591 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3467 4368 5045 6193 6438 1606 Size: 1861 901 677 1148 245 2120 Fragments arranged by size: 2120 1861 1148 901 677 245 NcoI C'CATG_G Cuts at: 513 1065 3301 513 Size: 552 2236 4164 Fragments arranged by size: 4164 2236 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6952 NheI G'CTAG_C Cuts at: 1052 3857 1052 Size: 2805 4147 NotI GC'GGCC_GC Cuts at: 4399 4399 Size: 6952 NsiI A_TGCA'T Cuts at: 2983 2983 Size: 6952 NspI r_CATG'y Cuts at: 1132 1229 2159 2812 4129 4310 5855 1132 Size: 97 930 653 1317 181 1545 2229 Fragments arranged by size: 2229 1545 1317 930 653 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6952 PciI A'CATG_T Cuts at: 2155 5851 2155 Size: 3696 3256 PflMI CCAn_nnn'nTGG Cuts at: 1108 2338 3002 3182 3319 1108 Size: 1230 664 180 137 4741 Fragments arranged by size: 4741 1230 664 180 137 PfoI T'CCnGG_A Cuts at: 3606 3997 3606 Size: 391 6561 PmeI GTTT'AAAC Cuts at: 4256 4256 Size: 6952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5021 5053 6534 6566 5021 Size: 32 1481 32 5407 Fragments arranged by size: 5407 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6952 PsiI TTA'TAA Cuts at: 4522 4522 Size: 6952 PstI C_TGCA'G Cuts at: 830 3066 4249 4304 830 Size: 2236 1183 55 3478 Fragments arranged by size: 3478 2236 1183 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6952 SalI G'TCGA_C Cuts at: 1936 3228 3590 4294 1936 Size: 1292 362 704 4594 Fragments arranged by size: 4594 1292 704 362 SbfI CC_TGCA'GG Cuts at: 4304 4304 Size: 6952 ScaI AGT'ACT Cuts at: 4201 5200 6912 4201 Size: 999 1712 4241 Fragments arranged by size: 4241 1712 999 SfcI C'TryA_G Cuts at: 826 1045 3062 3814 3954 4227 4245 4300 5435 Size: 219 2017 752 140 273 18 55 1135 Cuts at: 5435 6116 6307 826 Size: 681 191 1471 Fragments arranged by size: 2017 1471 1135 752 681 273 219 191 140 55 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6952 SmaI CCC'GGG Cuts at: 1872 4279 1872 Size: 2407 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2292 2985 4074 4139 5056 5957 Size: 197 926 349 693 1089 65 917 901 Cuts at: 5957 6219 820 Size: 262 1553 Fragments arranged by size: 1553 1089 926 917 901 693 349 262 197 65 SnaBI TAC'GTA Cuts at: 493 4743 493 Size: 4250 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6952 SphI G_CATG'C Cuts at: 1132 4129 4310 1132 Size: 2997 181 3774 Fragments arranged by size: 3774 2997 181 SspI AAT'ATT Cuts at: 5 52 3096 3433 4876 5 Size: 47 3044 337 1443 2081 Fragments arranged by size: 3044 2081 1443 337 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6952 StyI C'CwwG_G Cuts at: 513 1065 3301 3456 4391 513 Size: 552 2236 155 935 3074 Fragments arranged by size: 3074 2236 935 552 155 SwaI ATTT'AAAT Cuts at: 3984 3984 Size: 6952 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3613 4979 4996 5149 5334 5779 325 Size: 1255 2033 1366 17 153 185 445 1498 Fragments arranged by size: 2033 1498 1366 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2110 2256 2616 Size: 274 80 33 51 1443 133 146 360 Cuts at: 2616 4199 5198 6910 96 Size: 1583 999 1712 138 Fragments arranged by size: 1712 1583 1443 999 360 274 146 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6912 6944 6912 Size: 32 6920 Tth111I GACn'n_nGTC Cuts at: 1316 2023 2637 2660 1316 Size: 707 614 23 5608 Fragments arranged by size: 5608 707 614 23 XbaI T'CTAG_A Cuts at: 4288 4288 Size: 6952 XhoI C'TCGA_G Cuts at: 1943 2985 4139 1943 Size: 1042 1154 4756 Fragments arranged by size: 4756 1154 1042 XmaI C'CCGG_G Cuts at: 1870 4277 1870 Size: 2407 4545 XmnI GAAnn'nnTTC Cuts at: 5081 5081 Size: 6952 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3343 4760 276 Size: 53 83 186 936 1809 1417 2468 Fragments arranged by size: 2468 1809 1417 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI