Restriction map for
pFN21AB6234
(Circular) (Six-base) MAPSORT of: pFN21AB6234.seq Check: 2596 from: 1 to: 6736
pFN21AB6234 6736 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2775 2775
Size: 6736
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4546 278
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4079 1937
Size: 2142 4594
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2673 2815 3323 3375 4799 6183 6451 1632
Size: 1041 142 508 52 1424 1384 268 1917
Fragments arranged by size:
1917 1424 1384 1041 508 268 142 52
AfeI AGC'GCT
Cuts at: 3415 3415
Size: 6736
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 2388 3055 5635 6612 1203
Size: 1185 667 2580 977 1327
Fragments arranged by size:
2580 1327 1185 977 667
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 2219 2822 1166
Size: 1053 603 5080
Fragments arranged by size:
5080 1053 603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3185 1184
Size: 2001 4735
AlwNI CAG_nnn'CTG
Cuts at: 3780 4479 6051 3780
Size: 699 1572 4465
Fragments arranged by size:
4465 1572 699
ApaI G_GGCC'C
Cuts at: 2625 3181 2625
Size: 556 6180
ApaLI G'TGCA_C
Cuts at: 2357 2699 4793 5949 2357
Size: 342 2094 1156 3144
Fragments arranged by size:
3144 2094 1156 342
ApoI r'AATT_y
Cuts at: 1493 3389 4045 4271 4423 6532 1493
Size: 1896 656 226 152 2109 1697
Fragments arranged by size:
2109 1896 1697 656 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5290 160
Size: 872 4258 1606
Fragments arranged by size:
4258 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 2947 4061 1870
Size: 73 1004 1114 4545
Fragments arranged by size:
4545 1114 1004 73
BamHI G'GATC_C
Cuts at: 4066 4433 4066
Size: 367 6369
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 3497 3977 4057 5512
Size: 325 217 627 273 1437 480 80 1455
Cuts at: 5512 618
Size: 1842
Fragments arranged by size:
1842 1455 1437 627 480 325 273 217
80
BanII G_rGCy'C
Cuts at: 1391 2625 2687 3181 3342 4055 1391
Size: 1234 62 494 161 713 4072
Fragments arranged by size:
4072 1234 713 494 161 62
BbeI G_GCGC'C
Cuts at: 3501 3501
Size: 6736
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3852 928
Size: 971 1953 3812
Fragments arranged by size:
3812 1953 971
BbvCI CC'TCA_GC
Cuts at: 2466 2466
Size: 6736
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4927 4961 1903
Size: 34 2990 34 3678
Fragments arranged by size:
3678 2990 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 2325 1548
Size: 777 5959
BfrBI ATG'CAT
Cuts at: 3425 3425
Size: 6736
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2440 2841 3116
Size: 107 122 71 631 605 768 401 275
Cuts at: 3116 5347 136
Size: 2231 1525
Fragments arranged by size:
2231 1525 768 631 605 401 275 122
107 71
BglII A'GATC_T
Cuts at: 6731 6731
Size: 6736
BlpI GC'TnA_GC
Cuts at: 4153 4153
Size: 6736
Bme1580I G_kGCm'C
Cuts at: 1790 2065 2361 2625 2703 3181 4797 5953 1790
Size: 275 296 264 78 478 1616 1156 2573
Fragments arranged by size:
2573 1616 1156 478 296 275 264 78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6736
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2740 3497 4467
Size: 416 53 83 399 24 1323 757 970
Cuts at: 4467 5425 442
Size: 958 1753
Fragments arranged by size:
1753 1323 970 958 757 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3198 4021 3198
Size: 823 5913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2476 3110 3383 5396 1239
Size: 944 293 634 273 2013 2579
Fragments arranged by size:
2579 2013 944 634 293 273
Bpu10I CC'TnA_GC
Cuts at: 2066 2466 3965 2066
Size: 400 1499 4837
Fragments arranged by size:
4837 1499 400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3257 3326 4861 5726 6024 3257
Size: 69 1535 865 298 3969
Fragments arranged by size:
3969 1535 865 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2022 2372 2391 3701 5399 882
Size: 617 523 350 19 1310 1698 2219
Fragments arranged by size:
2219 1698 1310 617 523 350 19
BsaAI yAC'GTr
Cuts at: 493 1204 2755 4527 493
Size: 711 1551 1772 2702
Fragments arranged by size:
2702 1772 1551 711
BsaBI GATnn'nnATC
Cuts at: 6730 6730
Size: 6736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3498 4543 4925 5560
Size: 53 83 186 936 1965 1045 382 635
Cuts at: 5560 275
Size: 1451
Fragments arranged by size:
1965 1451 1045 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2731 3394 3951 5169 5567 5841 5988 6399 2731
Size: 663 557 1218 398 274 147 411 3068
Fragments arranged by size:
3068 1218 663 557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 3668 3698 1178
Size: 30 165 30 1149 30 1086 30 4216
Fragments arranged by size:
4216 1149 1086 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2935 3488 1186
Size: 1749 553 4434
Fragments arranged by size:
4434 1749 553
BseYI C'CCAG_C
Cuts at: 2113 2309 3773 4031 5939 6435 2113
Size: 196 1464 258 1908 496 2414
Fragments arranged by size:
2414 1908 1464 496 258 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2760 2996 3191 4040 1163
Size: 573 483 541 236 195 849 3859
Fragments arranged by size:
3859 849 573 541 483 236 195
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2912 4186 4947 5975 1540
Size: 275 186 911 1274 761 1028 2301
Fragments arranged by size:
2301 1274 1028 911 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2361 2703 3342 3732 4797 4882 5953 2361
Size: 342 639 390 1065 85 1071 3144
Fragments arranged by size:
3144 1071 1065 639 390 342 85
BsmI GAATG_Cn'
Cuts at: 3916 4247 4340 3916
Size: 331 93 6312
Fragments arranged by size:
6312 331 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2065 2361 2625 2687 2703 3181 3342
Size: 399 275 296 264 62 16 478 161
Cuts at: 3342 3732 4055 4797 4882 5953 1391
Size: 390 323 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 399 390 323 296
275 264 161 85 62 16
BspEI T'CCGG_A
Cuts at: 2731 3394 6399 2731
Size: 663 3005 3068
Fragments arranged by size:
3068 3005 663
BspHI T'CATG_A
Cuts at: 4625 6355 4625
Size: 1730 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2750 2775 4091 844
Size: 1906 25 1316 3489
Fragments arranged by size:
3489 1906 1316 25
BsrBI CCG'CTC
Cuts at: 1394 1823 2056 2077 4623 1394
Size: 429 233 21 2546 3507
Fragments arranged by size:
3507 2546 429 233 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 3031 5231 5405 65
Size: 1183 1783 2200 174 1396
Fragments arranged by size:
2200 1783 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2715 4026 5380 5567 1700
Size: 110 905 1311 1354 187 2869
Fragments arranged by size:
2869 1354 1311 905 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 3520 4796 5808 3520
Size: 1276 1012 4448
Fragments arranged by size:
4448 1276 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6736
BstBI TT'CG_AA
Cuts at: 5540 5540
Size: 6736
BstEII G'GTnAC_C
Cuts at: 1184 2762 3242 1184
Size: 1578 480 4678
Fragments arranged by size:
4678 1578 480
BstXI CCAn_nnnn'nTGG
Cuts at: 2393 3718 2393
Size: 1325 5411
BstYI r'GATC_y
Cuts at: 1972 4066 4433 4818 4835 6287 6373 6385 6731
Size: 2094 367 385 17 1452 86 12 346
Cuts at: 6731 1972
Size: 1977
Fragments arranged by size:
2094 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3260 3260
Size: 6736
BtgI C'CryG_G
Cuts at: 513 1065 2004 3615 513
Size: 552 939 1611 3634
Fragments arranged by size:
3634 1611 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2407 487
Size: 683 56 1181 4816
Fragments arranged by size:
4816 1181 683 56
BtsI GCAGTG_nn'
Cuts at: 3140 3601 3976 4028 4252 5046 5066 3140
Size: 461 375 52 224 794 20 4810
Fragments arranged by size:
4810 794 461 375 224 52 20
ClaI AT'CG_AT
Cuts at: 1554 3804 1554
Size: 2250 4486
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6701
DraI TTT'AAA
Cuts at: 4040 4040
Size: 6736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5743 809
Size: 507 4427 1802
Fragments arranged by size:
4427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2548 2909 3598
Size: 54 1425 50 275 18 718 361 689
Cuts at: 3598 3789 4183 5072 6701 8
Size: 191 394 889 1629 43
Fragments arranged by size:
1629 1425 889 718 689 394 361 275
191 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2909 4183 1537
Size: 275 1097 1274 4090
Fragments arranged by size:
4090 1274 1097 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3458 4666 1353
Size: 2105 1208 3423
Fragments arranged by size:
3423 2105 1208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2471 3497 5323 5697 5843 387
Size: 1064 138 882 1026 1826 374 146 1280
Fragments arranged by size:
1826 1280 1064 1026 882 374 146 138
EcoICRI GAG'CTC
Cuts at: 3340 3340
Size: 6736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2476 2673 2815 3110 3323 3375
Size: 393 551 293 197 142 295 213 52
Cuts at: 3375 3383 4799 5396 6183 6451 1239
Size: 8 1416 597 787 268 1524
Fragments arranged by size:
1524 1416 787 597 551 393 295 293
268 213 197 142 52 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2629 2629
Size: 6736
EcoO109I rG'GnC_Cy
Cuts at: 2227 2622 3177 3178 3485 3984 2227
Size: 395 555 1 307 499 4979
Fragments arranged by size:
4979 555 499 395 307 1
EcoRI G'AATT_C
Cuts at: 4045 4423 4045
Size: 378 6358
EcoRV GAT'ATC
Cuts at: 3622 3622
Size: 6736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3328 3328
Size: 6736
FspI TGC'GCA
Cuts at: 5242 5242
Size: 6736
HaeII r_GCGC'y
Cuts at: 1678 2083 2435 3417 3501 5883 1678
Size: 405 352 982 84 2382 2531
Fragments arranged by size:
2531 2382 982 405 352 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 3524 3556 3668
Size: 32 135 32 447 32 1473 32 112
Cuts at: 3668 3700 5377 5409 5451 5483 1373
Size: 32 1677 32 42 32 2626
Fragments arranged by size:
2626 1677 1473 447 135 112 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2017 2155 2581 2860 3754 4080
Size: 988 281 79 138 426 279 894 326
Cuts at: 4080 4326 669
Size: 246 3079
Fragments arranged by size:
3079 988 894 426 326 281 279 246
138 79
HindIII A'AGCT_T
Cuts at: 748 2777 748
Size: 2029 4707
HpaI GTT'AAC
Cuts at: 4326 4326
Size: 6736
KasI G'GCGC_C
Cuts at: 3497 3497
Size: 6736
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 4335 4335
Size: 6736
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3015 3903 5850 6034 1305
Size: 1710 888 1947 184 2007
Fragments arranged by size:
2007 1947 1710 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2550 3791 10
Size: 54 1425 343 718 1241 2955
Fragments arranged by size:
2955 1425 1241 718 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2387 2648 2822 3024 3252 4694
Size: 648 1053 168 261 174 202 228 1442
Cuts at: 4694 5053 5212 518
Size: 359 159 2042
Fragments arranged by size:
2042 1442 1053 648 359 261 228 202
174 168 159
MspA1I CmG'CkG
Cuts at: 1606 2233 2554 2619 2693 2908 3327 3446 3901
Size: 627 321 65 74 215 419 119 455
Cuts at: 3901 4152 4829 5977 6222 1606
Size: 251 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 627 455 419 321 251
245 215 119 74 65
NaeI GCC'GGC
Cuts at: 4028 4028
Size: 6736
NarI GG'CG_CC
Cuts at: 3498 3498
Size: 6736
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5245
Fragments arranged by size:
5245 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6736
NgoMIV G'CCGG_C
Cuts at: 4026 4026
Size: 6736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NsiI A_TGCA'T
Cuts at: 3427 3427
Size: 6736
NspI r_CATG'y
Cuts at: 1132 1229 2357 2392 3059 4094 5639 1132
Size: 97 1128 35 667 1035 1545 2229
Fragments arranged by size:
2229 1545 1128 1035 667 97 35
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6736
PciI A'CATG_T
Cuts at: 2388 3055 5635 2388
Size: 667 2580 3489
Fragments arranged by size:
3489 2580 667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 1108
Size: 1449 5287
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 6318 6350 4805
Size: 32 1481 32 5191
Fragments arranged by size:
5191 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 3485 2227
Size: 1258 5478
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 4306 4306
Size: 6736
PspOMI G'GGCC_C
Cuts at: 2621 3177 2621
Size: 556 6180
PstI C_TGCA'G
Cuts at: 830 2136 3765 4088 830
Size: 1306 1629 323 3478
Fragments arranged by size:
3478 1629 1306 323
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2554 3446 2554
Size: 892 5844
SacI G_AGCT'C
Cuts at: 3342 3342
Size: 6736
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SanDI GG'GwC_CC
Cuts at: 2227 2227
Size: 6736
SbfI CC_TGCA'GG
Cuts at: 4088 4088
Size: 6736
ScaI AGT'ACT
Cuts at: 2543 4984 6696 2543
Size: 2441 1712 2583
Fragments arranged by size:
2583 2441 1712
SexAI A'CCwGG_T
Cuts at: 3487 3717 3487
Size: 230 6506
SfcI C'TryA_G
Cuts at: 826 1045 2132 3761 4084 5219 5900 6091 826
Size: 219 1087 1629 323 1135 681 191 1471
Fragments arranged by size:
1629 1471 1135 1087 681 323 219 191
SfoI GGC'GCC
Cuts at: 3499 3499
Size: 6736
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 4063 1872
Size: 2191 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3272 3341 4840 5741 6003 820
Size: 197 926 1329 69 1499 901 262 1553
Fragments arranged by size:
1553 1499 1329 926 901 262 197 69
SnaBI TAC'GTA
Cuts at: 493 2755 4527 493
Size: 2262 1772 2702
Fragments arranged by size:
2702 2262 1772
SpeI A'CTAG_T
Cuts at: 152 2854 152
Size: 2702 4034
SphI G_CATG'C
Cuts at: 1132 4094 1132
Size: 2962 3774
SspI AAT'ATT
Cuts at: 5 52 4660 5
Size: 47 4608 2081
Fragments arranged by size:
4608 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6736
StyI C'CwwG_G
Cuts at: 513 1065 2004 3733 4175 513
Size: 552 939 1729 442 3074
Fragments arranged by size:
3074 1729 939 552 442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4763 4780 4933 5118 5563 325
Size: 1255 3183 17 153 185 445 1498
Fragments arranged by size:
3183 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 4982 6694
Size: 274 80 33 51 1443 564 2441 1712
Cuts at: 6694 96
Size: 138
Fragments arranged by size:
2441 1712 1443 564 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 6696 6728 2552
Size: 32 4112 32 2560
Fragments arranged by size:
4112 2560 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6736
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6736
XmaI C'CCGG_G
Cuts at: 1870 4061 1870
Size: 2191 4545
XmnI GAAnn'nnTTC
Cuts at: 4865 4865
Size: 6736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4544 276
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I DraIII
FseI FspAI NruI PacI PfoI PmlI PspXI PsrI
RsrII SacII SapI SfiI SrfI SwaI XcmI