Restriction map for
pFN21AB6233
(Circular) (Six-base) MAPSORT of: pFN21AB6233.seq Check: 6987 from: 1 to: 6949
pFN21AB6233 6949 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2044 3475 2044
Size: 1431 5518
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 278
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4292 1937
Size: 2355 4594
Acc65I G'GTAC_C
Cuts at: 4270 4270
Size: 6949
AclI AA'CG_TT
Cuts at: 1563 3293 5076 5449 1563
Size: 1730 1783 373 3063
Fragments arranged by size:
3063 1783 1730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 2391 2762 3294 3998 4266 5012 6396
Size: 626 133 371 532 704 268 746 1384
Cuts at: 6396 6664 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 746 704 626 532 371 268
268 133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6752
AflIII A'CryG_T
Cuts at: 1203 3417 5848 6825 1203
Size: 2214 2431 977 1327
Fragments arranged by size:
2431 2214 1327 977
AgeI A'CCGG_T
Cuts at: 5780 5780
Size: 6949
AhdI GACnn_n'nnGTC
Cuts at: 5678 5678
Size: 6949
AleI CACnn'nnGTG
Cuts at: 1166 3836 1166
Size: 2670 4279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6949
AlwNI CAG_nnn'CTG
Cuts at: 4692 6264 4692
Size: 1572 5377
ApaLI G'TGCA_C
Cuts at: 5006 6162 5006
Size: 1156 5793
ApoI r'AATT_y
Cuts at: 1493 2091 2282 2513 3197 4258 4484 4636 6745
Size: 598 191 231 684 1061 226 152 2109
Cuts at: 6745 1493
Size: 1697
Fragments arranged by size:
2109 1697 1061 684 598 231 226 191
152
AseI AT'TA_AT
Cuts at: 160 1032 5503 160
Size: 872 4471 1606
Fragments arranged by size:
4471 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 4274 1870
Size: 73 2331 4545
Fragments arranged by size:
4545 2331 73
BamHI G'GATC_C
Cuts at: 4279 4646 4279
Size: 367 6582
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3782 4270 5725 618
Size: 325 217 627 1995 488 1455 1842
Fragments arranged by size:
1995 1842 1455 627 488 325 217
BanII G_rGCy'C
Cuts at: 1391 2769 4268 1391
Size: 1378 1499 4072
Fragments arranged by size:
4072 1499 1378
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5978
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 1903
Size: 34 3203 34 3678
Fragments arranged by size:
3678 3203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4841 6051 4841
Size: 1210 5739
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6949
BfrBI ATG'CAT
Cuts at: 2302 2302
Size: 6949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3318 5560 136
Size: 107 122 71 631 605 1646 2242 1525
Fragments arranged by size:
2242 1646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2500 3306 6944 2500
Size: 806 3638 2505
Fragments arranged by size:
3638 2505 806
BlpI GC'TnA_GC
Cuts at: 4366 4366
Size: 6949
Bme1580I G_kGCm'C
Cuts at: 1790 5010 6166 1790
Size: 3220 1156 2573
Fragments arranged by size:
3220 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6949
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2140 4680 5638
Size: 416 53 83 399 24 723 2540 958
Cuts at: 5638 442
Size: 1753
Fragments arranged by size:
2540 1753 958 723 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3361 3361
Size: 6949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3258 3509 5609 1239
Size: 2019 251 2100 2579
Fragments arranged by size:
2579 2100 2019 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5074 5939 6237 5074
Size: 865 298 5786
Fragments arranged by size:
5786 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3332 5612 882
Size: 617 1833 2280 2219
Fragments arranged by size:
2280 2219 1833 617
BsaAI yAC'GTr
Cuts at: 493 1204 4740 493
Size: 711 3536 2702
Fragments arranged by size:
3536 2702 711
BsaBI GATnn'nnATC
Cuts at: 6943 6943
Size: 6949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4756 5138 5773 275
Size: 53 83 186 936 3223 382 635 1451
Fragments arranged by size:
3223 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5382 5780 6054 6201 6612 5382
Size: 398 274 147 411 5719
Fragments arranged by size:
5719 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6724
Fragments arranged by size:
6724 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2356 2858 1186
Size: 1170 502 5277
Fragments arranged by size:
5277 1170 502
BseYI C'CCAG_C
Cuts at: 6152 6648 6152
Size: 496 6453
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 1163
Size: 573 750 5626
Fragments arranged by size:
5626 750 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4399 5160 6188 1540
Size: 275 186 2398 761 1028 2301
Fragments arranged by size:
2398 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 5010 5095 6166 2439
Size: 2571 85 1071 3222
Fragments arranged by size:
3222 2571 1071 85
BsiWI C'GTAC_G
Cuts at: 3516 3516
Size: 6949
BsmI GAATG_Cn'
Cuts at: 4460 4553 4460
Size: 93 6856
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2769 4268 5010 5095 6166 1391
Size: 399 649 330 1499 742 85 1071 2174
Fragments arranged by size:
2174 1499 1071 742 649 399 330 85
BspEI T'CCGG_A
Cuts at: 6612 6612
Size: 6949
BspHI T'CATG_A
Cuts at: 4838 6568 4838
Size: 1730 5219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2044 3475 4304 844
Size: 1200 1431 829 3489
Fragments arranged by size:
3489 1431 1200 829
BsrBI CCG'CTC
Cuts at: 1394 1823 4836 1394
Size: 429 3013 3507
Fragments arranged by size:
3507 3013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3347 4230 5444 5618 65
Size: 1183 2099 883 1214 174 1396
Fragments arranged by size:
2099 1396 1214 1183 883 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3897 5593 5780 1700
Size: 110 2087 1696 187 2869
Fragments arranged by size:
2869 2087 1696 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6949
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6949
BssSI C'ACGA_G
Cuts at: 5009 6021 5009
Size: 1012 5937
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 794
Size: 1811 5138
BstBI TT'CG_AA
Cuts at: 5753 5753
Size: 6949
BstEII G'GTnAC_C
Cuts at: 1184 3596 1184
Size: 2412 4537
BstXI CCAn_nnnn'nTGG
Cuts at: 3334 3334
Size: 6949
BstYI r'GATC_y
Cuts at: 1972 2500 3306 3679 4279 4646 5031 5048 6500
Size: 528 806 373 600 367 385 17 1452
Cuts at: 6500 6586 6598 6944 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 806 600 528 385 373 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2085 2157 2571 513
Size: 552 1020 72 414 4891
Fragments arranged by size:
4891 1020 552 414 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6210
Fragments arranged by size:
6210 683 56
BtsI GCAGTG_nn'
Cuts at: 2594 4465 5259 5279 2594
Size: 1871 794 20 4264
Fragments arranged by size:
4264 1871 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4195 4230 529
Size: 35 3631 35 3248
Fragments arranged by size:
3631 3248 35 35
DraI TTT'AAA
Cuts at: 4253 4253
Size: 6949
DraIII CAC_nnn'GTG
Cuts at: 2443 3268 2443
Size: 825 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5956 809
Size: 507 4640 1802
Fragments arranged by size:
4640 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4396 5285 6914
Size: 54 1425 50 275 18 2566 889 1629
Cuts at: 6914 8
Size: 43
Fragments arranged by size:
2566 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4396 1537
Size: 275 2584 4090
Fragments arranged by size:
4090 2584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 2697 4879 1353
Size: 978 366 2182 3423
Fragments arranged by size:
3423 2182 978 366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5536 5910 6056 387
Size: 1064 138 3947 374 146 1280
Fragments arranged by size:
3947 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2391 2762 3258 3294 3509 3998
Size: 393 626 133 371 496 36 215 489
Cuts at: 3998 4266 5012 5609 6396 6664 1239
Size: 268 746 597 787 268 1524
Fragments arranged by size:
1524 787 746 626 597 496 489 393
371 268 268 215 133 36
EcoO109I rG'GnC_Cy
Cuts at: 2269 2378 2269
Size: 109 6840
EcoRI G'AATT_C
Cuts at: 4258 4636 4258
Size: 378 6571
FspI TGC'GCA
Cuts at: 5455 5455
Size: 6949
HaeII r_GCGC'y
Cuts at: 1678 6096 1678
Size: 4418 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2233 2265 2533
Size: 32 135 32 535 32 94 32 268
Cuts at: 2533 2565 2834 2866 5590 5622 5664 5696 1373
Size: 32 269 32 2724 32 42 32 2626
Fragments arranged by size:
2724 2626 535 269 268 135 94 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3423 3482 4293 4539 669
Size: 988 281 1485 59 811 246 3079
Fragments arranged by size:
3079 1485 988 811 281 246 59
HindIII A'AGCT_T
Cuts at: 748 3566 748
Size: 2818 4131
HpaI GTT'AAC
Cuts at: 4539 4539
Size: 6949
KpnI G_GTAC'C
Cuts at: 4274 4274
Size: 6949
MfeI C'AATT_G
Cuts at: 2828 3090 4212 4548 2828
Size: 262 1122 336 5229
Fragments arranged by size:
5229 1122 336 262
MluI A'CGCG_T
Cuts at: 6825 6825
Size: 6949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2050 2839 6063 6247 1305
Size: 745 789 3224 184 2007
Fragments arranged by size:
3224 2007 789 745 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5127
Fragments arranged by size:
5127 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2885 3836 4907 5266 5425 518
Size: 648 1719 951 1071 359 159 2042
Fragments arranged by size:
2042 1719 1071 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4365 5042 6190 6435 1606
Size: 2759 677 1148 245 2120
Fragments arranged by size:
2759 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2085 2157 2571 513
Size: 552 1020 72 414 4891
Fragments arranged by size:
4891 1020 552 414 72
NdeI CA'TA_TG
Cuts at: 387 2866 3552 387
Size: 2479 686 3784
Fragments arranged by size:
3784 2479 686
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 4396 4396
Size: 6949
NsiI A_TGCA'T
Cuts at: 2304 2304
Size: 6949
NspI r_CATG'y
Cuts at: 1132 1229 2306 2368 2905 4307 5852 1132
Size: 97 1077 62 537 1402 1545 2229
Fragments arranged by size:
2229 1545 1402 1077 537 97 62
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6949
PciI A'CATG_T
Cuts at: 5848 5848
Size: 6949
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3408 3442 3556 1108
Size: 2300 34 114 4501
Fragments arranged by size:
4501 2300 114 34
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5018 5050 6531 6563 5018
Size: 32 1481 32 5404
Fragments arranged by size:
5404 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2269
Size: 6949
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6949
PsiI TTA'TAA
Cuts at: 4519 4519
Size: 6949
PstI C_TGCA'G
Cuts at: 830 2359 2601 4301 830
Size: 1529 242 1700 3478
Fragments arranged by size:
3478 1700 1529 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6949
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SapI GCTCTTCn'nnn_
Cuts at: 2697 2697
Size: 6949
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 5197 6909 5197
Size: 1712 5237
SexAI A'CCwGG_T
Cuts at: 3634 3634
Size: 6949
SfcI C'TryA_G
Cuts at: 826 1045 2355 2521 2597 3586 3876 4027 4297
Size: 219 1310 166 76 989 290 151 270
Cuts at: 4297 5432 6113 6304 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1310 1135 989 681 290 270 219
191 166 151 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 4276 1872
Size: 2404 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5053 5954 6216 820
Size: 197 926 3110 901 262 1553
Fragments arranged by size:
3110 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4740 493
Size: 4247 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6949
SphI G_CATG'C
Cuts at: 1132 4307 1132
Size: 3175 3774
SspI AAT'ATT
Cuts at: 5 52 2349 3675 4873 5
Size: 47 2297 1326 1198 2081
Fragments arranged by size:
2297 2081 1326 1198 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6949
StyI C'CwwG_G
Cuts at: 513 1065 2085 2157 2571 4388 513
Size: 552 1020 72 414 1817 3074
Fragments arranged by size:
3074 1817 1020 552 414 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3826 4976 4993 5146 5331 5776 325
Size: 1255 2246 1150 17 153 185 445 1498
Fragments arranged by size:
2246 1498 1255 1150 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3130 5195 6907
Size: 274 80 33 51 1443 1153 2065 1712
Cuts at: 6907 96
Size: 138
Fragments arranged by size:
2065 1712 1443 1153 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6909 6941 6909
Size: 32 6917
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6949
XbaI T'CTAG_A
Cuts at: 2383 4285 2383
Size: 1902 5047
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3851 3851
Size: 6949
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6949
XmaI C'CCGG_G
Cuts at: 1870 4274 1870
Size: 2404 4545
XmnI GAAnn'nnTTC
Cuts at: 3279 3960 4123 5078 3279
Size: 681 163 955 5150
Fragments arranged by size:
5150 955 681 163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 276
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI