Restriction map for
pFN21AB6229
(Circular) (Six-base) MAPSORT of: pFN21AB6229.seq Check: 3821 from: 1 to: 6724
pFN21AB6229 6724 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2674 2734 2908 3621 2674
Size: 60 174 713 5777
Fragments arranged by size:
5777 713 174 60
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4534 278
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4067 1937
Size: 2130 4594
Acc65I G'GTAC_C
Cuts at: 2457 4045 2457
Size: 1588 5136
AclI AA'CG_TT
Cuts at: 1563 4851 5224 1563
Size: 3288 373 3063
Fragments arranged by size:
3288 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2544 2642 3091 4787 6171 6439 1632
Size: 749 163 98 449 1696 1384 268 1917
Fragments arranged by size:
1917 1696 1384 749 449 268 163 98
AfeI AGC'GCT
Cuts at: 3497 3833 3497
Size: 336 6388
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6527
AflIII A'CryG_T
Cuts at: 1203 5623 6600 1203
Size: 4420 977 1327
Fragments arranged by size:
4420 1327 977
AgeI A'CCGG_T
Cuts at: 5555 5555
Size: 6724
AhdI GACnn_n'nnGTC
Cuts at: 5453 5453
Size: 6724
AleI CACnn'nnGTG
Cuts at: 1166 3711 3795 1166
Size: 2545 84 4095
Fragments arranged by size:
4095 2545 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6724
AlwNI CAG_nnn'CTG
Cuts at: 2254 2359 4467 6039 2254
Size: 105 2108 1572 2939
Fragments arranged by size:
2939 2108 1572 105
ApaI G_GGCC'C
Cuts at: 2541 2676 2541
Size: 135 6589
ApaLI G'TGCA_C
Cuts at: 3405 3788 3898 4781 5937 3405
Size: 383 110 883 1156 4192
Fragments arranged by size:
4192 1156 883 383 110
ApoI r'AATT_y
Cuts at: 1493 4033 4259 4411 6520 1493
Size: 2540 226 152 2109 1697
Fragments arranged by size:
2540 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5278 160
Size: 872 4246 1606
Fragments arranged by size:
4246 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 3837 3965 4049 1870
Size: 73 732 1162 128 84 4545
Fragments arranged by size:
4545 1162 732 128 84 73
BamHI G'GATC_C
Cuts at: 4054 4421 4054
Size: 367 6357
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2457 2706 2755 2920 3374
Size: 325 217 627 670 249 49 165 454
Cuts at: 3374 3881 4045 5500 618
Size: 507 164 1455 1842
Fragments arranged by size:
1842 1455 670 627 507 454 325 249
217 165 164 49
BanII G_rGCy'C
Cuts at: 1391 2541 2558 2676 3312 4043 1391
Size: 1150 17 118 636 731 4072
Fragments arranged by size:
4072 1150 731 636 118 17
BbeI G_GCGC'C
Cuts at: 3885 3885
Size: 6724
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2735 3045 3299 3466 4004 928
Size: 971 836 310 254 167 538 3648
Fragments arranged by size:
3648 971 836 538 310 254 167
BbvCI CC'TCA_GC
Cuts at: 2721 2934 2721
Size: 213 6511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3396 3430 4915 4949 1903
Size: 34 1459 34 1485 34 3678
Fragments arranged by size:
3678 1485 1459 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4616 5826 4616
Size: 1210 5514
BclI T'GATC_A
Cuts at: 1548 3394 3855 1548
Size: 1846 461 4417
Fragments arranged by size:
4417 1846 461
BfrBI ATG'CAT
Cuts at: 2264 2264
Size: 6724
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2414 2516 5335
Size: 107 122 71 631 605 742 102 2819
Cuts at: 5335 136
Size: 1525
Fragments arranged by size:
2819 1525 742 631 605 122 107 102
71
BglII A'GATC_T
Cuts at: 6719 6719
Size: 6724
BlpI GC'TnA_GC
Cuts at: 4141 4141
Size: 6724
Bme1580I G_kGCm'C
Cuts at: 1790 2541 2676 2760 3409 3792 3902 4785 5941
Size: 751 135 84 649 383 110 883 1156
Cuts at: 5941 1790
Size: 2573
Fragments arranged by size:
2573 1156 883 751 649 383 135 110
84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6724
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4455 5413 442
Size: 416 53 83 399 24 3038 958 1753
Fragments arranged by size:
3038 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2351 2366 2390 3452 3791 4006 5384
Size: 1037 75 15 24 1062 339 215 1378
Cuts at: 5384 1239
Size: 2579
Fragments arranged by size:
2579 1378 1062 1037 339 215 75 24
15
Bpu10I CC'TnA_GC
Cuts at: 2721 2934 2964 2721
Size: 213 30 6481
Fragments arranged by size:
6481 213 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 3671 3743 4849 5714 6012 2447
Size: 1224 72 1106 865 298 3159
Fragments arranged by size:
3159 1224 1106 865 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2366 5387 882
Size: 617 867 3021 2219
Fragments arranged by size:
3021 2219 867 617
BsaAI yAC'GTr
Cuts at: 493 1204 3076 4515 493
Size: 711 1872 1439 2702
Fragments arranged by size:
2702 1872 1439 711
BsaBI GATnn'nnATC
Cuts at: 6718 6718
Size: 6724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3882 4531 4913 5548
Size: 53 83 186 936 2349 649 382 635
Cuts at: 5548 275
Size: 1451
Fragments arranged by size:
2349 1451 936 649 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2148 5157 5555 5829 5976 6387 2148
Size: 3009 398 274 147 411 2485
Fragments arranged by size:
3009 2485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2339 2354 2369 2489
Size: 30 165 30 921 15 15 15 120
Cuts at: 2489 2514 2519 2544 2625 2655 3818 3848 1178
Size: 25 5 25 81 30 1163 30 4054
Fragments arranged by size:
4054 1163 921 165 120 81 30 30
30 30 25 25 15 15 15 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2693 2918 3922 3983 1186
Size: 1324 183 225 1004 61 3927
Fragments arranged by size:
3927 1324 1004 225 183 61
BseYI C'CCAG_C
Cuts at: 2693 2759 2929 2971 3166 3587 3923 5927 6423
Size: 66 170 42 195 421 336 2004 496
Cuts at: 6423 2693
Size: 2994
Fragments arranged by size:
2994 2004 496 421 336 195 170 66
42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2149 3098 3241 3263 3892 1163
Size: 573 413 949 143 22 629 3995
Fragments arranged by size:
3995 949 629 573 413 143 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2322 4174 4935 5963 1540
Size: 275 186 321 1852 761 1028 2301
Fragments arranged by size:
2301 1852 1028 761 321 275 186
BsiHKAI G_wGCw'C
Cuts at: 2970 3312 3409 3683 3792 3902 4785 4870 5941
Size: 342 97 274 109 110 883 85 1071
Cuts at: 5941 2970
Size: 3753
Fragments arranged by size:
3753 1071 883 342 274 110 109 97
85
BsiWI C'GTAC_G
Cuts at: 3297 3297
Size: 6724
BsmI GAATG_Cn'
Cuts at: 2485 3354 4235 4328 2485
Size: 869 881 93 4881
Fragments arranged by size:
4881 881 869 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3767 3767
Size: 6724
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2541 2558 2676 2760 2970 3312 3409
Size: 399 751 17 118 84 210 342 97
Cuts at: 3409 3683 3792 3902 4043 4785 4870 5941 1391
Size: 274 109 110 141 742 85 1071 2174
Fragments arranged by size:
2174 1071 751 742 399 342 274 210
141 118 110 109 97 85 84 17
BspEI T'CCGG_A
Cuts at: 6387 6387
Size: 6724
BspHI T'CATG_A
Cuts at: 4613 6343 4613
Size: 1730 4994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 2674 2734 2908 3621 4079 844
Size: 1472 358 60 174 713 458 3489
Fragments arranged by size:
3489 1472 713 458 358 174 60
BsrBI CCG'CTC
Cuts at: 1394 1823 4611 1394
Size: 429 2788 3507
Fragments arranged by size:
3507 2788 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3739 5219 5393 65
Size: 1183 2491 1480 174 1396
Fragments arranged by size:
2491 1480 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5368 5555 1700
Size: 110 3558 187 2869
Fragments arranged by size:
3558 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6724
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6724
BssSI C'ACGA_G
Cuts at: 4784 5796 4784
Size: 1012 5712
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3570 3906 794
Size: 2776 336 3612
Fragments arranged by size:
3612 2776 336
BstBI TT'CG_AA
Cuts at: 3416 5528 3416
Size: 2112 4612
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6724
BstYI r'GATC_y
Cuts at: 1972 2008 2184 2325 3972 4054 4421 4806 4823
Size: 36 176 141 1647 82 367 385 17
Cuts at: 4823 6275 6361 6373 6719 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1647 1452 385 367 346 176 141
86 82 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2412 2541 3099 513
Size: 552 939 51 357 129 558 4138
Fragments arranged by size:
4138 939 558 552 357 129 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 487
Size: 683 56 895 5090
Fragments arranged by size:
5090 895 683 56
BtsI GCAGTG_nn'
Cuts at: 2616 3224 3515 3571 4240 5034 5054 2616
Size: 608 291 56 669 794 20 4286
Fragments arranged by size:
4286 794 669 608 291 56 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6724
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2557 2592 3721 3756 529
Size: 35 1993 35 1129 35 3497
Fragments arranged by size:
3497 1993 1129 35 35 35
DraI TTT'AAA
Cuts at: 4028 4028
Size: 6724
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3470 5731 809
Size: 507 2154 2261 1802
Fragments arranged by size:
2261 2154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2319 2415
Size: 54 1425 50 275 18 261 228 96
Cuts at: 2415 2941 2956 3468 3849 4171 5060 6689 8
Size: 526 15 512 381 322 889 1629 43
Fragments arranged by size:
1629 1425 889 526 512 381 322 275
261 228 96 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2319 4171 1537
Size: 275 507 1852 4090
Fragments arranged by size:
4090 1852 507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 2433 2661 4654 1353
Size: 842 238 228 1993 3423
Fragments arranged by size:
3423 1993 842 238 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2306 5311 5685 5831 387
Size: 1064 138 717 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 717 374 146 138
EcoICRI GAG'CTC
Cuts at: 3310 3310
Size: 6724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2351 2366 2381 2390 2544 2642
Size: 393 644 75 15 15 9 154 98
Cuts at: 2642 3091 3452 3791 4006 4787 5384 6171 6439
Size: 449 361 339 215 781 597 787 268
Cuts at: 6439 1239
Size: 1524
Fragments arranged by size:
1524 787 781 644 597 449 393 361
339 268 215 154 98 75 15 15
9
EcoO109I rG'GnC_Cy
Cuts at: 2296 2672 2840 2847 2947 2296
Size: 376 168 7 100 6073
Fragments arranged by size:
6073 376 168 100 7
EcoRI G'AATT_C
Cuts at: 4033 4411 4033
Size: 378 6346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 6724
FspI TGC'GCA
Cuts at: 3551 5230 3551
Size: 1679 5045
HaeII r_GCGC'y
Cuts at: 1678 3499 3835 3885 5871 1678
Size: 1821 336 50 1986 2531
Fragments arranged by size:
2531 1986 1821 336 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2514 2546 2625
Size: 32 135 32 641 32 269 32 79
Cuts at: 2625 2657 2839 2871 5365 5397 5439 5471 1373
Size: 32 182 32 2494 32 42 32 2626
Fragments arranged by size:
2626 2494 641 269 182 135 79 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2207 4068 4314 669
Size: 988 281 269 1861 246 3079
Fragments arranged by size:
3079 1861 988 281 269 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6724
HpaI GTT'AAC
Cuts at: 4314 4314
Size: 6724
KasI G'GCGC_C
Cuts at: 3881 3881
Size: 6724
KpnI G_GTAC'C
Cuts at: 2461 4049 2461
Size: 1588 5136
MfeI C'AATT_G
Cuts at: 4323 4323
Size: 6724
MluI A'CGCG_T
Cuts at: 6600 6600
Size: 6724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5838 6022 1305
Size: 4533 184 2007
Fragments arranged by size:
4533 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2943 10
Size: 54 1425 343 1111 3791
Fragments arranged by size:
3791 1425 1111 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 3464 3711 3795 4682 5041 5200
Size: 648 954 1344 247 84 887 359 159
Cuts at: 5200 518
Size: 2042
Fragments arranged by size:
2042 1344 954 887 648 359 247 159
84
MspA1I CmG'CkG
Cuts at: 1606 2035 2933 3371 3663 3769 4140 4817 5965
Size: 429 898 438 292 106 371 677 1148
Cuts at: 5965 6210 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 898 677 438 429 371 292
245 106
NarI GG'CG_CC
Cuts at: 3882 3882
Size: 6724
NcoI C'CATG_G
Cuts at: 513 1065 2004 2412 2541 3099 513
Size: 552 939 408 129 558 4138
Fragments arranged by size:
4138 939 558 552 408 129
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6724
NotI GC'GGCC_GC
Cuts at: 4171 4171
Size: 6724
NsiI A_TGCA'T
Cuts at: 2266 2266
Size: 6724
NspI r_CATG'y
Cuts at: 1132 1229 3049 4082 5627 1132
Size: 97 1820 1033 1545 2229
Fragments arranged by size:
2229 1820 1545 1033 97
PasI CC'CwG_GG
Cuts at: 1348 2534 2711 2774 3133 1348
Size: 1186 177 63 359 4939
Fragments arranged by size:
4939 1186 359 177 63
PciI A'CATG_T
Cuts at: 5623 5623
Size: 6724
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6724
PfoI T'CCnGG_A
Cuts at: 2328 3327 3669 2328
Size: 999 342 5383
Fragments arranged by size:
5383 999 342
PmeI GTTT'AAAC
Cuts at: 4028 4028
Size: 6724
PmlI CAC'GTG
Cuts at: 3076 3076
Size: 6724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 4793 4825 6306 6338 3635
Size: 32 1126 32 1481 32 4021
Fragments arranged by size:
4021 1481 1126 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2296 2847 2296
Size: 551 6173
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6724
PsiI TTA'TAA
Cuts at: 4294 4294
Size: 6724
PspOMI G'GGCC_C
Cuts at: 2537 2672 2537
Size: 135 6589
PstI C_TGCA'G
Cuts at: 830 2040 2611 2649 2671 3522 3566 4076 830
Size: 1210 571 38 22 851 44 510 3478
Fragments arranged by size:
3478 1210 851 571 510 44 38 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6724
PvuII CAG'CTG
Cuts at: 2035 2933 3663 3769 2035
Size: 898 730 106 4990
Fragments arranged by size:
4990 898 730 106
SacI G_AGCT'C
Cuts at: 3312 3312
Size: 6724
SalI G'TCGA_C
Cuts at: 1936 4066 1936
Size: 2130 4594
SanDI GG'GwC_CC
Cuts at: 2847 2847
Size: 6724
SbfI CC_TGCA'GG
Cuts at: 2649 2671 4076 2649
Size: 22 1405 5297
Fragments arranged by size:
5297 1405 22
ScaI AGT'ACT
Cuts at: 3917 4972 6684 3917
Size: 1055 1712 3957
Fragments arranged by size:
3957 1712 1055
SfcI C'TryA_G
Cuts at: 826 1045 2036 2607 2645 2667 3518 3562 3781
Size: 219 991 571 38 22 851 44 219
Cuts at: 3781 4072 5207 5888 6079 826
Size: 291 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 851 681 571 291 219
219 191 44 38 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 2414 2414
Size: 6724
SfoI GGC'GCC
Cuts at: 3883 3883
Size: 6724
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6724
SmaI CCC'GGG
Cuts at: 1872 3839 4051 1872
Size: 1967 212 4545
Fragments arranged by size:
4545 1967 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2462 3686 3758 4828 5729 5991
Size: 197 926 519 1224 72 1070 901 262
Cuts at: 5991 820
Size: 1553
Fragments arranged by size:
1553 1224 1070 926 901 519 262 197
72
SnaBI TAC'GTA
Cuts at: 493 4515 493
Size: 4022 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6724
SphI G_CATG'C
Cuts at: 1132 3049 4082 1132
Size: 1917 1033 3774
Fragments arranged by size:
3774 1917 1033
SspI AAT'ATT
Cuts at: 5 52 4648 5
Size: 47 4596 2081
Fragments arranged by size:
4596 2081 47
StuI AGG'CCT
Cuts at: 1517 2384 1517
Size: 867 5857
StyI C'CwwG_G
Cuts at: 513 1065 2004 2286 2412 2541 2601 3099 4163
Size: 552 939 282 126 129 60 498 1064
Cuts at: 4163 513
Size: 3074
Fragments arranged by size:
3074 1064 939 552 498 282 129 126
60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2985 4751 4768 4921 5106 5551 325
Size: 1255 1405 1766 17 153 185 445 1498
Fragments arranged by size:
1766 1498 1405 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3915 4970 6682
Size: 274 80 33 51 1443 1938 1055 1712
Cuts at: 6682 96
Size: 138
Fragments arranged by size:
1938 1712 1443 1055 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2339 2371 3818 3850 6684 6716 2339
Size: 32 1447 32 2834 32 2347
Fragments arranged by size:
2834 2347 1447 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6724
XbaI T'CTAG_A
Cuts at: 4060 4060
Size: 6724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2608 2771 2951 2608
Size: 163 180 6381
Fragments arranged by size:
6381 180 163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6724
XmaI C'CCGG_G
Cuts at: 1870 3837 4049 1870
Size: 1967 212 4545
Fragments arranged by size:
4545 1967 212
XmnI GAAnn'nnTTC
Cuts at: 2471 3323 3497 4853 2471
Size: 852 174 1356 4342
Fragments arranged by size:
4342 1356 852 174
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4532 276
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BplI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
PacI PspXI PsrI RsrII SacII SapI SexAI SrfI
SwaI