Restriction map for
pFN21AB6217
(Circular) (Six-base) MAPSORT of: pFN21AB6217.seq Check: 3580 from: 1 to: 6889
pFN21AB6217 6889 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3412 3412
Size: 6889
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4699 278
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4232 1937
Size: 2295 4594
Acc65I G'GTAC_C
Cuts at: 2598 2934 4210 2598
Size: 336 1276 5277
Fragments arranged by size:
5277 1276 336
AclI AA'CG_TT
Cuts at: 1563 5016 5389 1563
Size: 3453 373 3063
Fragments arranged by size:
3453 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2494 4952 6336 6604 1632
Size: 862 2458 1384 268 1917
Fragments arranged by size:
2458 1917 1384 862 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6692
AflIII A'CryG_T
Cuts at: 1203 5788 6765 1203
Size: 4585 977 1327
Fragments arranged by size:
4585 1327 977
AgeI A'CCGG_T
Cuts at: 5720 5720
Size: 6889
AhdI GACnn_n'nnGTC
Cuts at: 5618 5618
Size: 6889
AleI CACnn'nnGTG
Cuts at: 1166 2690 1166
Size: 1524 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6889
AlwNI CAG_nnn'CTG
Cuts at: 2272 2752 3221 3537 4073 4632 6204 2272
Size: 480 469 316 536 559 1572 2957
Fragments arranged by size:
2957 1572 559 536 480 469 316
ApaI G_GGCC'C
Cuts at: 2069 2279 2782 3229 4127 4188 2069
Size: 210 503 447 898 61 4770
Fragments arranged by size:
4770 898 503 447 210 61
ApaLI G'TGCA_C
Cuts at: 3239 4946 6102 3239
Size: 1707 1156 4026
Fragments arranged by size:
4026 1707 1156
ApoI r'AATT_y
Cuts at: 1493 4198 4424 4576 6685 1493
Size: 2705 226 152 2109 1697
Fragments arranged by size:
2705 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5443 160
Size: 872 4411 1606
Fragments arranged by size:
4411 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 3228 4214 1870
Size: 73 88 1197 986 4545
Fragments arranged by size:
4545 1197 986 88 73
BamHI G'GATC_C
Cuts at: 4219 4586 4219
Size: 367 6522
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2290 2390 2598 2934 4210
Size: 325 217 627 503 100 208 336 1276
Cuts at: 4210 5665 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1276 627 503 336 325 217
208 100
BanII G_rGCy'C
Cuts at: 1391 2069 2076 2121 2241 2279 2355 2782 2791
Size: 678 7 45 120 38 76 427 9
Cuts at: 2791 3229 3897 4127 4148 4188 4208 1391
Size: 438 668 230 21 40 20 4072
Fragments arranged by size:
4072 678 668 438 427 230 120 76
45 40 38 21 20 9 7
BbeI G_GCGC'C
Cuts at: 2294 2394 2294
Size: 100 6789
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2435 2551 3129 3679 928
Size: 971 536 116 578 550 4138
Fragments arranged by size:
4138 971 578 550 536 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5080 5114 1903
Size: 34 3143 34 3678
Fragments arranged by size:
3678 3143 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4781 5991 4781
Size: 1210 5679
BclI T'GATC_A
Cuts at: 1548 3456 1548
Size: 1908 4981
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2441 3975 5500
Size: 107 122 71 631 605 769 1534 1525
Cuts at: 5500 136
Size: 1525
Fragments arranged by size:
1534 1525 1525 769 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6884 6884
Size: 6889
BlpI GC'TnA_GC
Cuts at: 2075 3891 4306 2075
Size: 1816 415 4658
Fragments arranged by size:
4658 1816 415
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2069 2279 2782 3229 3243 4127 4188
Size: 270 9 210 503 447 14 884 61
Cuts at: 4188 4950 6106 1790
Size: 762 1156 2573
Fragments arranged by size:
2573 1156 884 762 503 447 270 210
61 14 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6889
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 4620 5578
Size: 416 53 83 399 24 1814 1389 958
Cuts at: 5578 442
Size: 1753
Fragments arranged by size:
1814 1753 1389 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6889
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 3817 4015 2227
Size: 1590 198 5101
Fragments arranged by size:
5101 1590 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2217 2219 2642 2788 3011 3545 3948
Size: 920 58 2 423 146 223 534 403
Cuts at: 3948 4068 4122 5549 1239
Size: 120 54 1427 2579
Fragments arranged by size:
2579 1427 920 534 423 403 223 146
120 58 54 2
Bpu10I CC'TnA_GC
Cuts at: 3987 3987
Size: 6889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5014 5879 6177 5014
Size: 865 298 5726
Fragments arranged by size:
5726 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2183 3046 3296 3966 5552 882
Size: 617 684 863 250 670 1586 2219
Fragments arranged by size:
2219 1586 863 684 670 617 250
BsaAI yAC'GTr
Cuts at: 493 1204 4680 493
Size: 711 3476 2702
Fragments arranged by size:
3476 2702 711
BsaBI GATnn'nnATC
Cuts at: 6883 6883
Size: 6889
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2291 2391 4696 5078
Size: 53 83 186 936 758 100 2305 382
Cuts at: 5078 5713 275
Size: 635 1451
Fragments arranged by size:
2305 1451 936 758 635 382 186 100
83 53
BsaWI w'CCGG_w
Cuts at: 5322 5720 5994 6141 6552 5322
Size: 398 274 147 411 5659
Fragments arranged by size:
5659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3026 3056 4084 4114 1178
Size: 30 165 30 1623 30 1028 30 3953
Fragments arranged by size:
3953 1623 1028 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2216 2276 2327 2330 2630 2639 3008
Size: 970 60 60 51 3 300 9 369
Cuts at: 3008 3565 3853 1186
Size: 557 288 4222
Fragments arranged by size:
4222 970 557 369 300 288 60 60
51 9 3
BseYI C'CCAG_C
Cuts at: 2272 2295 2412 3004 4062 6092 6588 2272
Size: 23 117 592 1058 2030 496 2573
Fragments arranged by size:
2573 2030 1058 592 496 117 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2443 2563 3346 1163
Size: 573 707 120 783 4706
Fragments arranged by size:
4706 783 707 573 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4339 5100 6128 1540
Size: 275 186 2338 761 1028 2301
Fragments arranged by size:
2338 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2355 2411 3243 3897 3988 4950 5035 6106
Size: 279 56 832 654 91 962 85 1071
Cuts at: 6106 2076
Size: 2859
Fragments arranged by size:
2859 1071 962 832 654 279 91 85
56
BsmI GAATG_Cn'
Cuts at: 4400 4493 4400
Size: 93 6796
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2069 2076 2121 2241 2279 2355
Size: 399 270 9 7 45 120 38 76
Cuts at: 2355 2411 2782 2791 3229 3243 3897 3988 4127
Size: 56 371 9 438 14 654 91 139
Cuts at: 4127 4148 4188 4208 4950 5035 6106 1391
Size: 21 40 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 654 438 399 371 270
139 120 91 85 76 56 45 40
38 21 20 14 9 9 7
BspEI T'CCGG_A
Cuts at: 6552 6552
Size: 6889
BspHI T'CATG_A
Cuts at: 2121 2241 4778 6508 2121
Size: 120 2537 1730 2502
Fragments arranged by size:
2537 2502 1730 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2293 2727 3265 3412 3915 4244 844
Size: 1449 434 538 147 503 329 3489
Fragments arranged by size:
3489 1449 538 503 434 329 147
BsrBI CCG'CTC
Cuts at: 1394 1823 2340 4776 1394
Size: 429 517 2436 3507
Fragments arranged by size:
3507 2436 517 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5384 5558 65
Size: 1183 4136 174 1396
Fragments arranged by size:
4136 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2696 5533 5720 1700
Size: 110 886 2837 187 2869
Fragments arranged by size:
2869 2837 886 187 110
BsrGI T'GTAC_A
Cuts at: 96 2664 96
Size: 2568 4321
BssHII G'CGCG_C
Cuts at: 1925 3178 1925
Size: 1253 5636
BssSI C'ACGA_G
Cuts at: 2690 4949 5961 2690
Size: 2259 1012 3618
Fragments arranged by size:
3618 2259 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2743 794
Size: 1949 4940
BstBI TT'CG_AA
Cuts at: 5693 5693
Size: 6889
BstEII G'GTnAC_C
Cuts at: 1184 3814 1184
Size: 2630 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2401 2692 2011
Size: 390 291 6208
Fragments arranged by size:
6208 390 291
BstYI r'GATC_y
Cuts at: 1972 4219 4586 4971 4988 6440 6526 6538 6884
Size: 2247 367 385 17 1452 86 12 346
Cuts at: 6884 1972
Size: 1977
Fragments arranged by size:
2247 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2753 2753
Size: 6889
BtgI C'CryG_G
Cuts at: 513 1065 2004 2304 2386 3491 513
Size: 552 939 300 82 1105 3911
Fragments arranged by size:
3911 1105 939 552 300 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6150
Fragments arranged by size:
6150 683 56
BtsI GCAGTG_nn'
Cuts at: 2451 2574 3382 4405 5199 5219 2451
Size: 123 808 1023 794 20 4121
Fragments arranged by size:
4121 1023 808 794 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3448 3483 529
Size: 35 2884 35 3935
Fragments arranged by size:
3935 2884 35 35
DraI TTT'AAA
Cuts at: 4193 4193
Size: 6889
DraIII CAC_nnn'GTG
Cuts at: 3603 3603
Size: 6889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2555 3911 5896 809
Size: 507 1239 1356 1985 1802
Fragments arranged by size:
1985 1802 1356 1239 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2694 3744
Size: 54 1425 50 275 18 183 681 1050
Cuts at: 3744 4336 5225 6854 8
Size: 592 889 1629 43
Fragments arranged by size:
1629 1425 1050 889 681 592 275 183
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4336 1537
Size: 275 2524 4090
Fragments arranged by size:
4090 2524 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 4819 1353
Size: 1007 2459 3423
Fragments arranged by size:
3423 2459 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2324 2387 4156 5476 5850 5996
Size: 1064 138 735 63 1769 1320 374 146
Cuts at: 5996 387
Size: 1280
Fragments arranged by size:
1769 1320 1280 1064 735 374 146 138
63
EcoICRI GAG'CTC
Cuts at: 2074 2353 3895 2074
Size: 279 1542 5068
Fragments arranged by size:
5068 1542 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2217 2219 2494 2642 2788 3011
Size: 393 527 58 2 275 148 146 223
Cuts at: 3011 3545 3948 4068 4122 4952 5549 6336 6604
Size: 534 403 120 54 830 597 787 268
Cuts at: 6604 1239
Size: 1524
Fragments arranged by size:
1524 830 787 597 534 527 403 393
275 268 223 148 146 120 58 54
2
EcoNI CCTnn'n_nnAGG
Cuts at: 2993 2993
Size: 6889
EcoO109I rG'GnC_Cy
Cuts at: 2065 2149 2276 2320 2758 2778 3225 4070 4124
Size: 84 127 44 438 20 447 845 54
Cuts at: 4124 4184 2065
Size: 60 4770
Fragments arranged by size:
4770 845 447 438 127 84 60 54
44 20
EcoRI G'AATT_C
Cuts at: 4198 4576 4198
Size: 378 6511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2575 2872 2452
Size: 123 297 6469
Fragments arranged by size:
6469 297 123
FspI TGC'GCA
Cuts at: 5395 5395
Size: 6889
HaeII r_GCGC'y
Cuts at: 1678 2294 2394 2752 6036 1678
Size: 616 100 358 3284 2531
Fragments arranged by size:
3284 2531 616 358 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 3560 3592 4084
Size: 32 135 32 509 32 1447 32 492
Cuts at: 4084 4116 5530 5562 5604 5636 1373
Size: 32 1414 32 42 32 2626
Fragments arranged by size:
2626 1447 1414 509 492 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3568 3724 3754 4233 4479 669
Size: 988 281 1630 156 30 479 246 3079
Fragments arranged by size:
3079 1630 988 479 281 246 156 30
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6889
HpaI GTT'AAC
Cuts at: 4479 4479
Size: 6889
KasI G'GCGC_C
Cuts at: 2290 2390 2290
Size: 100 6789
KpnI G_GTAC'C
Cuts at: 2602 2938 4214 2602
Size: 336 1276 5277
Fragments arranged by size:
5277 1276 336
MfeI C'AATT_G
Cuts at: 4488 4488
Size: 6889
MluI A'CGCG_T
Cuts at: 6765 6765
Size: 6889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3147 3852 6003 6187 1305
Size: 1842 705 2151 184 2007
Fragments arranged by size:
2151 2007 1842 705 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2015 3746 10
Size: 54 1425 343 183 1731 3153
Fragments arranged by size:
3153 1731 1425 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2690 3314 4847 5206 5365 518
Size: 648 843 681 624 1533 359 159 2042
Fragments arranged by size:
2042 1533 843 681 648 624 359 159
MspA1I CmG'CkG
Cuts at: 1606 2863 3493 4305 4982 6130 6375 1606
Size: 1257 630 812 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 812 677 630 245
NaeI GCC'GGC
Cuts at: 2698 2698
Size: 6889
NarI GG'CG_CC
Cuts at: 2291 2391 2291
Size: 100 6789
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5398
Fragments arranged by size:
5398 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6889
NgoMIV G'CCGG_C
Cuts at: 2696 2696
Size: 6889
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6889
NotI GC'GGCC_GC
Cuts at: 4336 4336
Size: 6889
NruI TCG'CGA
Cuts at: 2715 3703 2715
Size: 988 5901
NspI r_CATG'y
Cuts at: 1132 1229 4247 5792 1132
Size: 97 3018 1545 2229
Fragments arranged by size:
3018 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4120 1348
Size: 2772 4117
PciI A'CATG_T
Cuts at: 5788 5788
Size: 6889
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3129 3505 1108
Size: 2021 376 4492
Fragments arranged by size:
4492 2021 376
PfoI T'CCnGG_A
Cuts at: 3444 3444
Size: 6889
PmeI GTTT'AAAC
Cuts at: 4193 4193
Size: 6889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4958 4990 6471 6503 4958
Size: 32 1481 32 5344
Fragments arranged by size:
5344 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 2758 2149
Size: 609 6280
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6889
PsiI TTA'TAA
Cuts at: 4459 4459
Size: 6889
PspOMI G'GGCC_C
Cuts at: 2065 2275 2778 3225 4123 4184 2065
Size: 210 503 447 898 61 4770
Fragments arranged by size:
4770 898 503 447 210 61
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 6857
PstI C_TGCA'G
Cuts at: 830 2166 4153 4241 830
Size: 1336 1987 88 3478
Fragments arranged by size:
3478 1987 1336 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6889
PvuII CAG'CTG
Cuts at: 2863 2863
Size: 6889
SacI G_AGCT'C
Cuts at: 2076 2355 3897 2076
Size: 279 1542 5068
Fragments arranged by size:
5068 1542 279
SacII CC_GC'GG
Cuts at: 3494 3494
Size: 6889
SalI G'TCGA_C
Cuts at: 1936 4231 1936
Size: 2295 4594
SanDI GG'GwC_CC
Cuts at: 2758 2758
Size: 6889
SapI GCTCTTCn'nnn_
Cuts at: 2360 2360
Size: 6889
SbfI CC_TGCA'GG
Cuts at: 4241 4241
Size: 6889
ScaI AGT'ACT
Cuts at: 2507 3512 5137 6849 2507
Size: 1005 1625 1712 2547
Fragments arranged by size:
2547 1712 1625 1005
SexAI A'CCwGG_T
Cuts at: 2173 3036 2173
Size: 863 6026
SfcI C'TryA_G
Cuts at: 826 1045 2162 2593 3520 4149 4237 5372 6053
Size: 219 1117 431 927 629 88 1135 681
Cuts at: 6053 6244 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1117 927 681 629 431 219
191 88
SfoI GGC'GCC
Cuts at: 2292 2392 2292
Size: 100 6789
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6889
SmaI CCC'GGG
Cuts at: 1872 3230 4216 1872
Size: 1358 986 4545
Fragments arranged by size:
4545 1358 986
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4993 5894 6156 820
Size: 197 926 3050 901 262 1553
Fragments arranged by size:
3050 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4680 493
Size: 4187 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6889
SphI G_CATG'C
Cuts at: 1132 4247 1132
Size: 3115 3774
SspI AAT'ATT
Cuts at: 5 52 4813 5
Size: 47 4761 2081
Fragments arranged by size:
4761 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6889
StyI C'CwwG_G
Cuts at: 513 1065 2004 4328 513
Size: 552 939 2324 3074
Fragments arranged by size:
3074 2324 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4916 4933 5086 5271 5716 325
Size: 1255 3336 17 153 185 445 1498
Fragments arranged by size:
3336 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2475 2505 2664
Size: 274 80 33 51 1443 498 30 159
Cuts at: 2664 3411 3510 5135 6847 96
Size: 747 99 1625 1712 138
Fragments arranged by size:
1712 1625 1443 747 498 274 159 138
99 80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2020 2052 2242 2274 2572 2604 6849 6881 2020
Size: 32 190 32 298 32 4245 32 2028
Fragments arranged by size:
4245 2028 298 190 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6889
XbaI T'CTAG_A
Cuts at: 4225 4225
Size: 6889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3083 3641 3083
Size: 558 6331
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6889
XmaI C'CCGG_G
Cuts at: 1870 3228 4214 1870
Size: 1358 986 4545
Fragments arranged by size:
4545 1358 986
XmnI GAAnn'nnTTC
Cuts at: 3194 5018 3194
Size: 1824 5065
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4697 276
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI NsiI PacI PmlI
PspXI RsrII SfiI SrfI SwaI