Restriction map for
pFN21AB6214
(Circular) (Six-base) MAPSORT of: pFN21AB6214.seq Check: 2409 from: 1 to: 6880
pFN21AB6214 6880 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4690 278
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4223 1937
Size: 2286 4594
Acc65I G'GTAC_C
Cuts at: 3373 4201 3373
Size: 828 6052
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2264 2460 2576 3762 4943 6327 6595 1632
Size: 632 196 116 1186 1181 1384 268 1917
Fragments arranged by size:
1917 1384 1186 1181 632 268 196 116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6683
AflIII A'CryG_T
Cuts at: 1203 5779 6756 1203
Size: 4576 977 1327
Fragments arranged by size:
4576 1327 977
AgeI A'CCGG_T
Cuts at: 5711 5711
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 5609 5609
Size: 6880
AleI CACnn'nnGTG
Cuts at: 1166 3593 1166
Size: 2427 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6880
AlwNI CAG_nnn'CTG
Cuts at: 4623 6195 4623
Size: 1572 5308
ApaI G_GGCC'C
Cuts at: 2202 2202
Size: 6880
ApaLI G'TGCA_C
Cuts at: 4937 6093 4937
Size: 1156 5724
ApoI r'AATT_y
Cuts at: 1493 3338 3565 4189 4415 4567 6676 1493
Size: 1845 227 624 226 152 2109 1697
Fragments arranged by size:
2109 1845 1697 624 227 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 2427 4205 1870
Size: 73 484 1778 4545
Fragments arranged by size:
4545 1778 484 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2966 2999 2966
Size: 33 6847
BamHI G'GATC_C
Cuts at: 4210 4577 4210
Size: 367 6513
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 4201 5656 618
Size: 325 217 627 1586 828 1455 1842
Fragments arranged by size:
1842 1586 1455 828 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 4199 1391
Size: 811 1997 4072
Fragments arranged by size:
4072 1997 811
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3186 928
Size: 971 1287 4622
Fragments arranged by size:
4622 1287 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5071 5105 1903
Size: 34 3134 34 3678
Fragments arranged by size:
3678 3134 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2476 3784 3964 4772 5982 2476
Size: 1308 180 808 1210 3374
Fragments arranged by size:
3374 1308 1210 808 180
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5491 136
Size: 107 122 71 631 605 3819 1525
Fragments arranged by size:
3819 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6875 6875
Size: 6880
BlpI GC'TnA_GC
Cuts at: 4297 4297
Size: 6880
Bme1580I G_kGCm'C
Cuts at: 1790 2202 4941 6097 1790
Size: 412 2739 1156 2573
Fragments arranged by size:
2739 2573 1156 412
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6880
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2445 2791 4611
Size: 416 53 83 399 24 1028 346 1820
Cuts at: 4611 5569 442
Size: 958 1753
Fragments arranged by size:
1820 1753 1028 958 416 399 346 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2086 5540 1239
Size: 847 3454 2579
Fragments arranged by size:
3454 2579 847
Bpu10I CC'TnA_GC
Cuts at: 3630 3630
Size: 6880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2055 3246 3472 5005 5870 6168 2055
Size: 1191 226 1533 865 298 2767
Fragments arranged by size:
2767 1533 1191 865 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3530 5543 882
Size: 617 2031 2013 2219
Fragments arranged by size:
2219 2031 2013 617
BsaAI yAC'GTr
Cuts at: 493 1204 4671 493
Size: 711 3467 2702
Fragments arranged by size:
3467 2702 711
BsaBI GATnn'nnATC
Cuts at: 3924 6874 3924
Size: 2950 3930
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4687 5069 5704 275
Size: 53 83 186 936 3154 382 635 1451
Fragments arranged by size:
3154 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2558 5313 5711 5985 6132 6543 2558
Size: 2755 398 274 147 411 2895
Fragments arranged by size:
2895 2755 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2849 2879 3453 3483 3632
Size: 30 165 30 1446 30 574 30 149
Cuts at: 3632 3662 1178
Size: 30 4396
Fragments arranged by size:
4396 1446 574 165 149 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3640 3649 1186
Size: 2454 9 4417
Fragments arranged by size:
4417 2454 9
BseYI C'CCAG_C
Cuts at: 2761 6083 6579 2761
Size: 3322 496 3062
Fragments arranged by size:
3322 3062 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4040 1163
Size: 573 2304 4003
Fragments arranged by size:
4003 2304 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4330 5091 6119 1540
Size: 275 186 2329 761 1028 2301
Fragments arranged by size:
2329 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2071 4941 5026 6097 2071
Size: 2870 85 1071 2854
Fragments arranged by size:
2870 2854 1071 85
BsmI GAATG_Cn'
Cuts at: 2568 4391 4484 2568
Size: 1823 93 4964
Fragments arranged by size:
4964 1823 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2202 4199 4941 5026 6097 1391
Size: 399 281 131 1997 742 85 1071 2174
Fragments arranged by size:
2174 1997 1071 742 399 281 131 85
BspEI T'CCGG_A
Cuts at: 6543 6543
Size: 6880
BspHI T'CATG_A
Cuts at: 3142 4769 6499 3142
Size: 1627 1730 3523
Fragments arranged by size:
3523 1730 1627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2910 4235 844
Size: 2066 1325 3489
Fragments arranged by size:
3489 2066 1325
BsrBI CCG'CTC
Cuts at: 1394 1823 4767 1394
Size: 429 2944 3507
Fragments arranged by size:
3507 2944 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2537 3942 5375 5549 65
Size: 1183 1289 1405 1433 174 1396
Fragments arranged by size:
1433 1405 1396 1289 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5524 5711 1700
Size: 110 3714 187 2869
Fragments arranged by size:
3714 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3109 96
Size: 3013 3867
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 2157 4940 5952 2157
Size: 2783 1012 3085
Fragments arranged by size:
3085 2783 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6880
BstBI TT'CG_AA
Cuts at: 5684 5684
Size: 6880
BstEII G'GTnAC_C
Cuts at: 1184 3902 1184
Size: 2718 4162
BstXI CCAn_nnnn'nTGG
Cuts at: 3595 3595
Size: 6880
BstYI r'GATC_y
Cuts at: 1972 4210 4577 4962 4979 6431 6517 6529 6875
Size: 2238 367 385 17 1452 86 12 346
Cuts at: 6875 1972
Size: 1977
Fragments arranged by size:
2238 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2397 2397
Size: 6880
BtgI C'CryG_G
Cuts at: 513 1065 2004 2665 3897 513
Size: 552 939 661 1232 3496
Fragments arranged by size:
3496 1232 939 661 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6141
Fragments arranged by size:
6141 683 56
BtsI GCAGTG_nn'
Cuts at: 4396 5190 5210 4396
Size: 794 20 6066
Fragments arranged by size:
6066 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6845
DraI TTT'AAA
Cuts at: 4184 4184
Size: 6880
DraIII CAC_nnn'GTG
Cuts at: 2732 2732
Size: 6880
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2749 5887 809
Size: 507 1433 3138 1802
Fragments arranged by size:
3138 1802 1433 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4327 5216 6845
Size: 54 1425 50 275 18 2497 889 1629
Cuts at: 6845 8
Size: 43
Fragments arranged by size:
2497 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4327 1537
Size: 275 2515 4090
Fragments arranged by size:
4090 2515 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 2436 2595 3201 4810 1353
Size: 693 390 159 606 1609 3423
Fragments arranged by size:
3423 1609 693 606 390 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5467 5841 5987 387
Size: 1064 138 3878 374 146 1280
Fragments arranged by size:
3878 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2264 2460 2576 3762 4943 5540
Size: 393 454 178 196 116 1186 1181 597
Cuts at: 5540 6327 6595 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1186 1181 787 597 454 393 268
196 178 116
EcoO109I rG'GnC_Cy
Cuts at: 2198 2199 2585 2742 2198
Size: 1 386 157 6336
Fragments arranged by size:
6336 386 157 1
EcoRI G'AATT_C
Cuts at: 4189 4567 4189
Size: 378 6502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2594 2940 3091 2594
Size: 346 151 6383
Fragments arranged by size:
6383 346 151
FspI TGC'GCA
Cuts at: 5386 5386
Size: 6880
HaeII r_GCGC'y
Cuts at: 1678 6027 1678
Size: 4349 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2203 2235 2380 2412 4037
Size: 32 135 32 631 32 145 32 1625
Cuts at: 4037 4069 5521 5553 5595 5627 1373
Size: 32 1452 32 42 32 2626
Fragments arranged by size:
2626 1625 1452 631 145 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2035 4224 4470 669
Size: 988 281 97 2189 246 3079
Fragments arranged by size:
3079 2189 988 281 246 97
HindIII A'AGCT_T
Cuts at: 748 3051 748
Size: 2303 4577
HpaI GTT'AAC
Cuts at: 4470 4470
Size: 6880
KpnI G_GTAC'C
Cuts at: 3377 4205 3377
Size: 828 6052
MfeI C'AATT_G
Cuts at: 4479 4479
Size: 6880
MluI A'CGCG_T
Cuts at: 6756 6756
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3428 3937 5994 6178 1305
Size: 2123 509 2057 184 2007
Fragments arranged by size:
2123 2057 2007 509 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5058
Fragments arranged by size:
5058 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2617 3432 3593 4091 4838 5197 5356
Size: 648 1451 815 161 498 747 359 159
Cuts at: 5356 518
Size: 2042
Fragments arranged by size:
2042 1451 815 747 648 498 359 161
159
MspA1I CmG'CkG
Cuts at: 1606 3740 4296 4973 6121 6366 1606
Size: 2134 556 677 1148 245 2120
Fragments arranged by size:
2134 2120 1148 677 556 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3897 513
Size: 552 939 1893 3496
Fragments arranged by size:
3496 1893 939 552
NdeI CA'TA_TG
Cuts at: 387 2809 387
Size: 2422 4458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6880
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NspI r_CATG'y
Cuts at: 1132 1229 3064 3867 4238 5783 1132
Size: 97 1835 803 371 1545 2229
Fragments arranged by size:
2229 1835 1545 803 371 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6880
PciI A'CATG_T
Cuts at: 5779 5779
Size: 6880
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 2813 1108
Size: 1137 568 5175
Fragments arranged by size:
5175 1137 568
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4949 4981 6462 6494 4949
Size: 32 1481 32 5335
Fragments arranged by size:
5335 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2742 2742
Size: 6880
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6880
PsiI TTA'TAA
Cuts at: 4450 4450
Size: 6880
PspOMI G'GGCC_C
Cuts at: 2198 2198
Size: 6880
PstI C_TGCA'G
Cuts at: 830 2230 3220 4232 830
Size: 1400 990 1012 3478
Fragments arranged by size:
3478 1400 1012 990
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
PvuII CAG'CTG
Cuts at: 3740 3740
Size: 6880
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SanDI GG'GwC_CC
Cuts at: 2742 2742
Size: 6880
SbfI CC_TGCA'GG
Cuts at: 4232 4232
Size: 6880
ScaI AGT'ACT
Cuts at: 3886 5128 6840 3886
Size: 1242 1712 3926
Fragments arranged by size:
3926 1712 1242
SfcI C'TryA_G
Cuts at: 826 1045 2136 2226 3003 3216 3510 3689 4176
Size: 219 1091 90 777 213 294 179 487
Cuts at: 4176 4228 5363 6044 6235 826
Size: 52 1135 681 191 1471
Fragments arranged by size:
1471 1135 1091 777 681 487 294 219
213 191 179 90 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 4207 1872
Size: 2335 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2070 3261 3451 4984 5885 6147
Size: 197 926 127 1191 190 1533 901 262
Cuts at: 6147 820
Size: 1553
Fragments arranged by size:
1553 1533 1191 926 901 262 197 190
127
SnaBI TAC'GTA
Cuts at: 493 4671 493
Size: 4178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 4238 1132
Size: 3106 3774
SspI AAT'ATT
Cuts at: 5 52 3526 3678 4804 5
Size: 47 3474 152 1126 2081
Fragments arranged by size:
3474 2081 1126 152 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6880
StyI C'CwwG_G
Cuts at: 513 1065 2004 3710 3897 4319 513
Size: 552 939 1706 187 422 3074
Fragments arranged by size:
3074 1706 939 552 422 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4907 4924 5077 5262 5707 325
Size: 1255 3327 17 153 185 445 1498
Fragments arranged by size:
3327 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3109 3884 5126
Size: 274 80 33 51 1443 1132 775 1242
Cuts at: 5126 6838 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1242 1132 775 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 6840 6872 3632
Size: 32 3176 32 3640
Fragments arranged by size:
3640 3176 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6880
XbaI T'CTAG_A
Cuts at: 4216 4216
Size: 6880
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6880
XmaI C'CCGG_G
Cuts at: 1870 4205 1870
Size: 2335 4545
XmnI GAAnn'nnTTC
Cuts at: 2577 5009 2577
Size: 2432 4448
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4688 276
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI