Restriction map for
pFN21AB6210
(Circular) (Six-base) MAPSORT of: pFN21AB6210.seq Check: 6121 from: 1 to: 6871
pFN21AB6210 6871 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2238 2322 2556 2238
Size: 84 234 6553
Fragments arranged by size:
6553 234 84
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4681 278
Size: 53 83 186 936 3145 2468
Fragments arranged by size:
3145 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4214 1937
Size: 2277 4594
Acc65I G'GTAC_C
Cuts at: 4192 4192
Size: 6871
AclI AA'CG_TT
Cuts at: 1563 4998 5371 1563
Size: 3435 373 3063
Fragments arranged by size:
3435 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3492 4934 6318 6586 1632
Size: 1860 1442 1384 268 1917
Fragments arranged by size:
1917 1860 1442 1384 268
AfeI AGC'GCT
Cuts at: 2198 3084 2198
Size: 886 5985
AflII C'TTAA_G
Cuts at: 820 1017 2954 820
Size: 197 1937 4737
Fragments arranged by size:
4737 1937 197
AflIII A'CryG_T
Cuts at: 1203 4050 5770 6747 1203
Size: 2847 1720 977 1327
Fragments arranged by size:
2847 1720 1327 977
AgeI A'CCGG_T
Cuts at: 5702 5702
Size: 6871
AhdI GACnn_n'nnGTC
Cuts at: 5600 5600
Size: 6871
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6871
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2157 2189 2933 2965 2157
Size: 32 744 32 6063
Fragments arranged by size:
6063 744 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2789 4614 6186 2789
Size: 1825 1572 3474
Fragments arranged by size:
3474 1825 1572
ApaI G_GGCC'C
Cuts at: 2993 3080 3328 3412 2993
Size: 87 248 84 6452
Fragments arranged by size:
6452 248 87 84
ApaLI G'TGCA_C
Cuts at: 2972 3560 4004 4928 6084 2972
Size: 588 444 924 1156 3759
Fragments arranged by size:
3759 1156 924 588 444
ApoI r'AATT_y
Cuts at: 1493 4180 4406 4558 6667 1493
Size: 2687 226 152 2109 1697
Fragments arranged by size:
2687 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5425 160
Size: 872 4393 1606
Fragments arranged by size:
4393 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6871
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2123 2330 2648 2798 3004 3130
Size: 73 86 94 207 318 150 206 126
Cuts at: 3130 3252 3327 3370 3677 4068 4196 1870
Size: 122 75 43 307 391 128 4545
Fragments arranged by size:
4545 391 318 307 207 206 150 128
126 122 94 86 75 73 43
BamHI G'GATC_C
Cuts at: 4201 4568 4201
Size: 367 6504
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3264 3485 3608 3773 3932
Size: 325 217 627 1477 221 123 165 159
Cuts at: 3932 4192 5647 618
Size: 260 1455 1842
Fragments arranged by size:
1842 1477 1455 627 325 260 221 217
165 159 123
BanII G_rGCy'C
Cuts at: 1391 2015 2251 2299 2416 2647 2760 2808 2946
Size: 624 236 48 117 231 113 48 138
Cuts at: 2946 2993 3080 3129 3198 3259 3328 3347 3412
Size: 47 87 49 69 61 69 19 65
Cuts at: 3412 3976 3984 4124 4190 1391
Size: 564 8 140 66 4072
Fragments arranged by size:
4072 624 564 236 231 140 138 117
113 87 69 69 66 65 61 49
48 48 47 19 8
BbeI G_GCGC'C
Cuts at: 3489 3777 3489
Size: 288 6583
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5900
BbvCI CC'TCA_GC
Cuts at: 2688 2694 2700 2688
Size: 6 6 6859
Fragments arranged by size:
6859 6 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5062 5096 1903
Size: 34 3125 34 3678
Fragments arranged by size:
3678 3125 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4763 5973 4763
Size: 1210 5661
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6871
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2642 3141 5482
Size: 107 122 71 631 605 970 499 2341
Cuts at: 5482 136
Size: 1525
Fragments arranged by size:
2341 1525 970 631 605 499 122 107
71
BglII A'GATC_T
Cuts at: 6866 6866
Size: 6871
BlpI GC'TnA_GC
Cuts at: 3192 3529 4288 3192
Size: 337 759 5775
Fragments arranged by size:
5775 759 337
Bme1580I G_kGCm'C
Cuts at: 1790 2267 2976 2993 3004 3080 3328 3390 3412
Size: 477 709 17 11 76 248 62 22
Cuts at: 3412 3564 3611 3937 4008 4932 6088 1790
Size: 152 47 326 71 924 1156 2573
Fragments arranged by size:
2573 1156 924 709 477 326 248 152
76 71 62 47 22 17 11
BmgBI CAC'GTC
Cuts at: 1318 2018 1318
Size: 700 6171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2414 3607 4089
Size: 416 53 83 399 24 997 1193 482
Cuts at: 4089 4602 5560 442
Size: 513 958 1753
Fragments arranged by size:
1753 1193 997 958 513 482 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6871
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3793 4138 3793
Size: 345 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2581 3753 3990 5531 1239
Size: 1148 194 1172 237 1541 2579
Fragments arranged by size:
2579 1541 1172 1148 237 194
Bpu10I CC'TnA_GC
Cuts at: 2422 2688 2694 2700 3149 2422
Size: 266 6 6 449 6144
Fragments arranged by size:
6144 449 266 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3611 3763 4996 5861 6159 2855
Size: 756 152 1233 865 298 3567
Fragments arranged by size:
3567 1233 865 756 298 152
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 5534 882
Size: 617 2580 1455 2219
Fragments arranged by size:
2580 2219 1455 617
BsaAI yAC'GTr
Cuts at: 493 1204 2342 4662 493
Size: 711 1138 2320 2702
Fragments arranged by size:
2702 2320 1138 711
BsaBI GATnn'nnATC
Cuts at: 6865 6865
Size: 6871
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3486 3774 4678 5060
Size: 53 83 186 936 1953 288 904 382
Cuts at: 5060 5695 275
Size: 635 1451
Fragments arranged by size:
1953 1451 936 904 635 382 288 186
83 53
BsaWI w'CCGG_w
Cuts at: 2818 2917 5304 5702 5976 6123 6534 2818
Size: 99 2387 398 274 147 411 3155
Fragments arranged by size:
3155 2387 411 398 274 147 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2770 2800 2882 2912 2933
Size: 30 165 30 1367 30 82 30 21
Cuts at: 2933 2963 1178
Size: 30 5086
Fragments arranged by size:
5086 1367 165 82 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 2624 2816 3077 3290 3308 3326 3676
Size: 1435 3 192 261 213 18 18 350
Cuts at: 3676 4130 1186
Size: 454 3927
Fragments arranged by size:
3927 1435 454 350 261 213 192 18
18 3
BseYI C'CCAG_C
Cuts at: 2712 2736 2809 2986 3036 3079 3340 3404 3708
Size: 24 73 177 50 43 261 64 304
Cuts at: 3708 3806 4153 6074 6570 2712
Size: 98 347 1921 496 3013
Fragments arranged by size:
3013 1921 496 347 304 261 177 98
73 64 50 43 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2178 2337 2534 2603 2605 3344 1163
Size: 573 442 159 197 69 2 739 4690
Fragments arranged by size:
4690 739 573 442 197 159 69 2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3114 3208 3426 4321 5082
Size: 275 186 928 185 94 218 895 761
Cuts at: 5082 6110 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 928 895 761 275 218 186
185 94
BsiHKAI G_wGCw'C
Cuts at: 2976 3564 3984 4008 4932 5017 6088 2976
Size: 588 420 24 924 85 1071 3759
Fragments arranged by size:
3759 1071 924 588 420 85 24
BsmI GAATG_Cn'
Cuts at: 4382 4475 4382
Size: 93 6778
BsmBI CGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 6871
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2251 2267 2299 2416 2647 2760
Size: 399 225 236 16 32 117 231 113
Cuts at: 2760 2808 2946 2976 2993 3004 3080 3129 3198
Size: 48 138 30 17 11 76 49 69
Cuts at: 3198 3259 3328 3347 3390 3412 3564 3611 3937
Size: 61 69 19 43 22 152 47 326
Cuts at: 3937 3976 3984 4008 4124 4190 4932 5017 6088
Size: 39 8 24 116 66 742 85 1071
Cuts at: 6088 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 326 236 231 225
152 138 117 116 113 85 76 69
69 66 61 49 48 47 43 39
32 30 24 22 19 17 16 11
8
BspEI T'CCGG_A
Cuts at: 2818 6534 2818
Size: 3716 3155
BspHI T'CATG_A
Cuts at: 4760 6490 4760
Size: 1730 5141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2322 2556 3324 4226 844
Size: 1394 84 234 768 902 3489
Fragments arranged by size:
3489 1394 902 768 234 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2034 2135 2795 3528 3574 3666 4758
Size: 429 211 101 660 733 46 92 1092
Cuts at: 4758 1394
Size: 3507
Fragments arranged by size:
3507 1092 733 660 429 211 101 92
46
BsrDI GCAATG_nn'
Cuts at: 65 1248 5366 5540 65
Size: 1183 4118 174 1396
Fragments arranged by size:
4118 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 3171 3261 3491 3671 4032 5515
Size: 110 246 1115 90 230 180 361 1483
Cuts at: 5515 5702 1700
Size: 187 2869
Fragments arranged by size:
2869 1483 1115 361 246 230 187 180
110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6871
BssHII G'CGCG_C
Cuts at: 1925 2380 2382 1925
Size: 455 2 6414
Fragments arranged by size:
6414 455 2
BssSI C'ACGA_G
Cuts at: 4931 5943 4931
Size: 1012 5859
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2557 794
Size: 1763 5108
BstBI TT'CG_AA
Cuts at: 5675 5675
Size: 6871
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6871
BstXI CCAn_nnnn'nTGG
Cuts at: 2986 2986
Size: 6871
BstYI r'GATC_y
Cuts at: 1972 2077 4201 4568 4953 4970 6422 6508 6520
Size: 105 2124 367 385 17 1452 86 12
Cuts at: 6520 6866 1972
Size: 346 1977
Fragments arranged by size:
2124 1977 1452 385 367 346 105 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3282 3282
Size: 6871
BtgI C'CryG_G
Cuts at: 513 1065 2004 2638 513
Size: 552 939 634 4746
Fragments arranged by size:
4746 939 634 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6132
Fragments arranged by size:
6132 683 56
BtsI GCAGTG_nn'
Cuts at: 2269 2993 3592 4387 5181 5201 2269
Size: 724 599 795 794 20 3939
Fragments arranged by size:
3939 795 794 724 599 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6871
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6836
DraI TTT'AAA
Cuts at: 4175 4175
Size: 6871
DraIII CAC_nnn'GTG
Cuts at: 2999 2999
Size: 6871
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5878 809
Size: 507 4562 1802
Fragments arranged by size:
4562 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2275 2664
Size: 54 1425 50 275 18 205 240 389
Cuts at: 2664 2842 2926 3111 3205 4034 4318 5207 6836
Size: 178 84 185 94 829 284 889 1629
Cuts at: 6836 8
Size: 43
Fragments arranged by size:
1629 1425 889 829 389 284 275 240
205 185 178 94 84 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 3111 3205 4318 1537
Size: 275 1114 185 94 1113 4090
Fragments arranged by size:
4090 1114 1113 275 185 94
EarI CTCTTCn'nnn_
Cuts at: 1353 2370 3285 3432 3956 4801 1353
Size: 1017 915 147 524 845 3423
Fragments arranged by size:
3423 1017 915 845 524 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2476 2546 2673 3215 5458 5832
Size: 1064 138 887 70 127 542 2243 374
Cuts at: 5832 5978 387
Size: 146 1280
Fragments arranged by size:
2243 1280 1064 887 542 374 146 138
127 70
EcoICRI GAG'CTC
Cuts at: 3982 3982
Size: 6871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2581 3492 3753 3990 4934 5531
Size: 393 755 194 911 261 237 944 597
Cuts at: 5531 6318 6586 1239
Size: 787 268 1524
Fragments arranged by size:
1524 944 911 787 755 597 393 268
261 237 194
EcoO109I rG'GnC_Cy
Cuts at: 2565 3008 3408 3462 3816 3846 2565
Size: 443 400 54 354 30 5590
Fragments arranged by size:
5590 443 400 354 54 30
EcoRI G'AATT_C
Cuts at: 4180 4558 4180
Size: 378 6493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 6871
FseI GG_CCGG'CC
Cuts at: 3175 3675 3175
Size: 500 6371
FspI TGC'GCA
Cuts at: 2318 3854 5377 2318
Size: 1536 1523 3812
Fragments arranged by size:
3812 1536 1523
HaeII r_GCGC'y
Cuts at: 1678 2048 2173 2200 2338 2816 3086 3121 3489
Size: 370 125 27 138 478 270 35 368
Cuts at: 3489 3646 3777 6018 1678
Size: 157 131 2241 2531
Fragments arranged by size:
2531 2241 478 370 368 270 157 138
131 125 35 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2770 2802 5512 5544 5586
Size: 32 135 32 1198 32 2710 32 42
Cuts at: 5586 5618 1373
Size: 32 2626
Fragments arranged by size:
2710 2626 1198 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4215 4461 669
Size: 988 281 2277 246 3079
Fragments arranged by size:
3079 2277 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6871
HpaI GTT'AAC
Cuts at: 4461 4461
Size: 6871
KasI G'GCGC_C
Cuts at: 3485 3773 3485
Size: 288 6583
KpnI G_GTAC'C
Cuts at: 4196 4196
Size: 6871
MfeI C'AATT_G
Cuts at: 4470 4470
Size: 6871
MluI A'CGCG_T
Cuts at: 4050 6747 4050
Size: 2697 4174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2352 3436 3803 5985 6169 1305
Size: 813 234 1084 367 2182 184 2007
Fragments arranged by size:
2182 2007 1084 813 367 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5049
Fragments arranged by size:
5049 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 4829 5188 5347 518
Size: 648 1803 1860 359 159 2042
Fragments arranged by size:
2042 1860 1803 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2293 2675 2829 2931 3232 3712 3945 4287
Size: 687 382 154 102 301 480 233 342
Cuts at: 4287 4964 6112 6357 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 480 382 342 301
245 233 154 102
NaeI GCC'GGC
Cuts at: 2058 3173 3263 3673 4034 2058
Size: 1115 90 410 361 4895
Fragments arranged by size:
4895 1115 410 361 90
NarI GG'CG_CC
Cuts at: 3486 3774 3486
Size: 288 6583
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5380
Fragments arranged by size:
5380 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6871
NgoMIV G'CCGG_C
Cuts at: 2056 3171 3261 3671 4032 2056
Size: 1115 90 410 361 4895
Fragments arranged by size:
4895 1115 410 361 90
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6871
NotI GC'GGCC_GC
Cuts at: 2926 4318 2926
Size: 1392 5479
NspI r_CATG'y
Cuts at: 1132 1229 2434 2884 4229 5774 1132
Size: 97 1205 450 1345 1545 2229
Fragments arranged by size:
2229 1545 1345 1205 450 97
PasI CC'CwG_GG
Cuts at: 1348 3839 1348
Size: 2491 4380
PciI A'CATG_T
Cuts at: 5770 5770
Size: 6871
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6871
PmeI GTTT'AAAC
Cuts at: 4175 4175
Size: 6871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2933 2965 4940 4972 6453 6485 2933
Size: 32 1975 32 1481 32 3319
Fragments arranged by size:
3319 1975 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3462 3816 3846 3462
Size: 354 30 6487
Fragments arranged by size:
6487 354 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6871
PsiI TTA'TAA
Cuts at: 4441 4441
Size: 6871
PspOMI G'GGCC_C
Cuts at: 2989 3076 3324 3408 2989
Size: 87 248 84 6452
Fragments arranged by size:
6452 248 87 84
PstI C_TGCA'G
Cuts at: 830 2085 4223 830
Size: 1255 2138 3478
Fragments arranged by size:
3478 2138 1255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6871
SacI G_AGCT'C
Cuts at: 3984 3984
Size: 6871
SalI G'TCGA_C
Cuts at: 1936 4213 1936
Size: 2277 4594
SanDI GG'GwC_CC
Cuts at: 3462 3816 3462
Size: 354 6517
SapI GCTCTTCn'nnn_
Cuts at: 2370 2370
Size: 6871
SbfI CC_TGCA'GG
Cuts at: 4223 4223
Size: 6871
ScaI AGT'ACT
Cuts at: 5119 6831 5119
Size: 1712 5159
SfcI C'TryA_G
Cuts at: 826 1045 2081 3638 4219 5354 6035 6226 826
Size: 219 1036 1557 581 1135 681 191 1471
Fragments arranged by size:
1557 1471 1135 1036 681 581 219 191
SfoI GGC'GCC
Cuts at: 3487 3775 3487
Size: 288 6583
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6871
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6871
SmaI CCC'GGG
Cuts at: 1872 3006 3254 3329 3372 3679 4198 1872
Size: 1134 248 75 43 307 519 4545
Fragments arranged by size:
4545 1134 519 307 248 75 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 2954 3626 3778 4975 5876
Size: 197 926 927 84 672 152 1197 901
Cuts at: 5876 6138 820
Size: 262 1553
Fragments arranged by size:
1553 1197 927 926 901 672 262 197
152 84
SnaBI TAC'GTA
Cuts at: 493 4662 493
Size: 4169 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6871
SphI G_CATG'C
Cuts at: 1132 2434 4229 1132
Size: 1302 1795 3774
Fragments arranged by size:
3774 1795 1302
SrfI GCCC'GGGC
Cuts at: 3254 3254
Size: 6871
SspI AAT'ATT
Cuts at: 5 52 4795 5
Size: 47 4743 2081
Fragments arranged by size:
4743 2081 47
StuI AGG'CCT
Cuts at: 1517 2255 2897 1517
Size: 738 642 5491
Fragments arranged by size:
5491 738 642
StyI C'CwwG_G
Cuts at: 513 1065 2004 4310 513
Size: 552 939 2306 3074
Fragments arranged by size:
3074 2306 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2256 3626 3754 4898 4915 5068 5253
Size: 1255 676 1370 128 1144 17 153 185
Cuts at: 5253 5698 325
Size: 445 1498
Fragments arranged by size:
1498 1370 1255 1144 676 445 185 153
128 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5117 6829 96
Size: 274 80 33 51 1443 3140 1712 138
Fragments arranged by size:
3140 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2882 2914 6831 6863 2882
Size: 32 3917 32 2890
Fragments arranged by size:
3917 2890 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6871
XbaI T'CTAG_A
Cuts at: 4207 4207
Size: 6871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 2455
Size: 6871
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6871
XmaI C'CCGG_G
Cuts at: 1870 3004 3252 3327 3370 3677 4196 1870
Size: 1134 248 75 43 307 519 4545
Fragments arranged by size:
4545 1134 519 307 248 75 43
XmnI GAAnn'nnTTC
Cuts at: 2198 5000 2198
Size: 2802 4069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4679 276
Size: 53 83 186 936 3145 2468
Fragments arranged by size:
3145 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI EcoRV
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacII SexAI SfiI SwaI