Restriction map for
pFN21AB6205
(Circular) (Six-base) MAPSORT of: pFN21AB6205.seq Check: 9575 from: 1 to: 6697
pFN21AB6205 6697 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4507 278
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3313 4040 1937
Size: 1376 727 4594
Fragments arranged by size:
4594 1376 727
Acc65I G'GTAC_C
Cuts at: 4018 4018
Size: 6697
AclI AA'CG_TT
Cuts at: 1563 4824 5197 1563
Size: 3261 373 3063
Fragments arranged by size:
3261 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 3339 3555 4760 6144 6412 1632
Size: 893 814 216 1205 1384 268 1917
Fragments arranged by size:
1917 1384 1205 893 814 268 216
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6500
AflIII A'CryG_T
Cuts at: 1203 5596 6573 1203
Size: 4393 977 1327
Fragments arranged by size:
4393 1327 977
AgeI A'CCGG_T
Cuts at: 5528 5528
Size: 6697
AhdI GACnn_n'nnGTC
Cuts at: 5426 5426
Size: 6697
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6697
AlwNI CAG_nnn'CTG
Cuts at: 2801 2977 3253 4440 6012 2801
Size: 176 276 1187 1572 3486
Fragments arranged by size:
3486 1572 1187 276 176
ApaLI G'TGCA_C
Cuts at: 4754 5910 4754
Size: 1156 5541
ApoI r'AATT_y
Cuts at: 1493 3259 3383 4006 4232 4384 6493 1493
Size: 1766 124 623 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 623 226 152 124
AseI AT'TA_AT
Cuts at: 160 1032 3831 3887 5251 160
Size: 872 2799 56 1364 1606
Fragments arranged by size:
2799 1606 1364 872 56
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
AvaI C'yCGr_G
Cuts at: 1870 1943 3573 4022 1870
Size: 73 1630 449 4545
Fragments arranged by size:
4545 1630 449 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3645 3678 3645
Size: 33 6664
BamHI G'GATC_C
Cuts at: 3865 4027 4394 3865
Size: 162 367 6168
Fragments arranged by size:
6168 367 162
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 4018 5473 618
Size: 325 217 627 309 1922 1455 1842
Fragments arranged by size:
1922 1842 1455 627 325 309 217
BanII G_rGCy'C
Cuts at: 1391 2155 2601 4016 1391
Size: 764 446 1415 4072
Fragments arranged by size:
4072 1415 764 446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2489 3018 928
Size: 971 590 529 4607
Fragments arranged by size:
4607 971 590 529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4888 4922 1903
Size: 34 50 34 2867 34 3678
Fragments arranged by size:
3678 2867 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4589 5799 4589
Size: 1210 5487
BclI T'GATC_A
Cuts at: 1548 3391 1548
Size: 1843 4854
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5308 136
Size: 107 122 71 631 605 3636 1525
Fragments arranged by size:
3636 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3132 6692 3132
Size: 3560 3137
BlpI GC'TnA_GC
Cuts at: 4114 4114
Size: 6697
Bme1580I G_kGCm'C
Cuts at: 1790 2557 4758 5914 1790
Size: 767 2201 1156 2573
Fragments arranged by size:
2573 2201 1156 767
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6697
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4428 5386 442
Size: 416 53 83 399 24 3011 958 1753
Fragments arranged by size:
3011 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2415 1056
Size: 1359 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2778 2979 3053 3109 3783 5357 1239
Size: 872 667 201 74 56 674 1574 2579
Fragments arranged by size:
2579 1574 872 674 667 201 74 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3642 4822 5687 5985 3642
Size: 1180 865 298 4354
Fragments arranged by size:
4354 1180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5360 882
Size: 617 3861 2219
Fragments arranged by size:
3861 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4488 493
Size: 711 3284 2702
Fragments arranged by size:
3284 2702 711
BsaBI GATnn'nnATC
Cuts at: 3960 6691 3960
Size: 2731 3966
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4504 4886 5521 275
Size: 53 83 186 936 2971 382 635 1451
Fragments arranged by size:
2971 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5130 5528 5802 5949 6360 5130
Size: 398 274 147 411 5467
Fragments arranged by size:
5467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2549 3107 3137 3397
Size: 30 165 30 1116 30 558 30 260
Cuts at: 3397 3427 1178
Size: 30 4448
Fragments arranged by size:
4448 1116 558 260 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2524 3341 1186
Size: 862 476 817 4542
Fragments arranged by size:
4542 862 817 476
BseYI C'CCAG_C
Cuts at: 2754 3242 5900 6396 2754
Size: 488 2658 496 3055
Fragments arranged by size:
3055 2658 496 488
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6124
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4147 4908 5936 1540
Size: 275 186 2146 761 1028 2301
Fragments arranged by size:
2301 2146 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4758 4843 5914 4758
Size: 85 1071 5541
Fragments arranged by size:
5541 1071 85
BsmI GAATG_Cn'
Cuts at: 2709 4208 4301 2709
Size: 1499 93 5105
Fragments arranged by size:
5105 1499 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2557 2601 4016 4758 4843 5914
Size: 399 365 402 44 1415 742 85 1071
Cuts at: 5914 1391
Size: 2174
Fragments arranged by size:
2174 1415 1071 742 402 399 365 85
44
BspEI T'CCGG_A
Cuts at: 6360 6360
Size: 6697
BspHI T'CATG_A
Cuts at: 4586 6316 4586
Size: 1730 4967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4052 844
Size: 3208 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3185 4584 1394
Size: 429 1362 1399 3507
Fragments arranged by size:
3507 1399 1362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2139 2422 5192 5366 65
Size: 1183 891 283 2770 174 1396
Fragments arranged by size:
2770 1396 1183 891 283 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3015 5341 5528 1700
Size: 110 1205 2326 187 2869
Fragments arranged by size:
2869 2326 1205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6697
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6697
BssSI C'ACGA_G
Cuts at: 4757 5769 4757
Size: 1012 5685
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6697
BstBI TT'CG_AA
Cuts at: 5501 5501
Size: 6697
BstEII G'GTnAC_C
Cuts at: 1184 2849 2881 3619 1184
Size: 1665 32 738 4262
Fragments arranged by size:
4262 1665 738 32
BstXI CCAn_nnnn'nTGG
Cuts at: 2281 2668 2846 2281
Size: 387 178 6132
Fragments arranged by size:
6132 387 178
BstYI r'GATC_y
Cuts at: 1972 2368 2395 3132 3646 3865 4027 4394 4779
Size: 396 27 737 514 219 162 367 385
Cuts at: 4779 4796 6248 6334 6346 6692 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 737 514 396 385 367 346
219 162 86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 3153 3153
Size: 6697
BtgI C'CryG_G
Cuts at: 513 1065 2013 2508 2617 2952 3000 513
Size: 552 948 495 109 335 48 4210
Fragments arranged by size:
4210 948 552 495 335 109 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3566 487
Size: 683 56 2340 3618
Fragments arranged by size:
3618 2340 683 56
BtsI GCAGTG_nn'
Cuts at: 3201 4213 5007 5027 3201
Size: 1012 794 20 4871
Fragments arranged by size:
4871 1012 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6662
DraI TTT'AAA
Cuts at: 2391 4001 2391
Size: 1610 5087
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5704 809
Size: 507 4388 1802
Fragments arranged by size:
4388 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2505 2586
Size: 54 1425 50 275 18 342 333 81
Cuts at: 2586 2808 2997 4144 5033 6662 8
Size: 222 189 1147 889 1629 43
Fragments arranged by size:
1629 1425 1147 889 342 333 275 222
189 81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4144 1537
Size: 275 2332 4090
Fragments arranged by size:
4090 2332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3090 4627 1353
Size: 1737 1537 3423
Fragments arranged by size:
3423 1737 1537
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2533 2750 5284 5658 5804 387
Size: 1064 138 944 217 2534 374 146 1280
Fragments arranged by size:
2534 1280 1064 944 374 217 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2525 2778 2979 3053 3109 3339
Size: 393 479 414 253 201 74 56 230
Cuts at: 3339 3555 3783 4760 5357 6144 6412 1239
Size: 216 228 977 597 787 268 1524
Fragments arranged by size:
1524 977 787 597 479 414 393 268
253 230 228 216 201 74 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2896 2030
Size: 866 5831
EcoO109I rG'GnC_Cy
Cuts at: 2974 2974
Size: 6697
EcoRI G'AATT_C
Cuts at: 3383 4006 4384 3383
Size: 623 378 5696
Fragments arranged by size:
5696 623 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2783 3485 2783
Size: 702 5995
FspI TGC'GCA
Cuts at: 5203 5203
Size: 6697
HaeII r_GCGC'y
Cuts at: 1678 3022 5844 1678
Size: 1344 2822 2531
Fragments arranged by size:
2822 2531 1344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2952 2972 2984 3004 3428
Size: 32 135 32 1380 20 12 20 424
Cuts at: 3428 3460 5338 5370 5412 5444 1373
Size: 32 1878 32 42 32 2626
Fragments arranged by size:
2626 1878 1380 424 135 42 32 32
32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4041 4287 669
Size: 988 281 2103 246 3079
Fragments arranged by size:
3079 2103 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6697
HpaI GTT'AAC
Cuts at: 4287 4287
Size: 6697
KpnI G_GTAC'C
Cuts at: 4022 4022
Size: 6697
MfeI C'AATT_G
Cuts at: 4296 4296
Size: 6697
MluI A'CGCG_T
Cuts at: 6573 6573
Size: 6697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2473 5811 5995 1305
Size: 1168 3338 184 2007
Fragments arranged by size:
3338 2007 1168 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 2588 10
Size: 54 1425 343 342 414 4119
Fragments arranged by size:
4119 1425 414 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4655 5014 5173 518
Size: 648 3489 359 159 2042
Fragments arranged by size:
3489 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2510 4113 4790 5938 6183 1606
Size: 516 388 1603 677 1148 245 2120
Fragments arranged by size:
2120 1603 1148 677 516 388 245
NcoI C'CATG_G
Cuts at: 513 1065 2617 513
Size: 552 1552 4593
Fragments arranged by size:
4593 1552 552
NdeI CA'TA_TG
Cuts at: 387 2206 387
Size: 1819 4878
NheI G'CTAG_C
Cuts at: 1052 2411 1052
Size: 1359 5338
NotI GC'GGCC_GC
Cuts at: 4144 4144
Size: 6697
NruI TCG'CGA
Cuts at: 3164 3164
Size: 6697
NspI r_CATG'y
Cuts at: 1132 1229 4055 5600 1132
Size: 97 2826 1545 2229
Fragments arranged by size:
2826 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2943 1348
Size: 1595 5102
PciI A'CATG_T
Cuts at: 5596 5596
Size: 6697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2892 2977 3616 1108
Size: 1784 85 639 4189
Fragments arranged by size:
4189 1784 639 85
PfoI T'CCnGG_A
Cuts at: 2970 2970
Size: 6697
PmeI GTTT'AAAC
Cuts at: 4001 4001
Size: 6697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4798 6279 6311 4766
Size: 32 1481 32 5152
Fragments arranged by size:
5152 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2974 2974
Size: 6697
PshAI GACnn'nnGTC
Cuts at: 1613 2489 1613
Size: 876 5821
PsiI TTA'TAA
Cuts at: 4267 4267
Size: 6697
PstI C_TGCA'G
Cuts at: 830 2571 4049 830
Size: 1741 1478 3478
Fragments arranged by size:
3478 1741 1478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6697
PvuII CAG'CTG
Cuts at: 2122 2122
Size: 6697
SacII CC_GC'GG
Cuts at: 2511 2511
Size: 6697
SalI G'TCGA_C
Cuts at: 1936 4039 1936
Size: 2103 4594
SbfI CC_TGCA'GG
Cuts at: 4049 4049
Size: 6697
ScaI AGT'ACT
Cuts at: 2546 4945 6657 2546
Size: 2399 1712 2586
Fragments arranged by size:
2586 2399 1712
SfcI C'TryA_G
Cuts at: 826 1045 2567 3378 4045 5180 5861 6052 826
Size: 219 1522 811 667 1135 681 191 1471
Fragments arranged by size:
1522 1471 1135 811 681 667 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6697
SmaI CCC'GGG
Cuts at: 1872 4024 1872
Size: 2152 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3657 4801 5702 5964 820
Size: 197 926 1714 1144 901 262 1553
Fragments arranged by size:
1714 1553 1144 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4488 493
Size: 3995 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6697
SphI G_CATG'C
Cuts at: 1132 4055 1132
Size: 2923 3774
SspI AAT'ATT
Cuts at: 5 52 4621 5
Size: 47 4569 2081
Fragments arranged by size:
4569 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6697
StyI C'CwwG_G
Cuts at: 513 1065 2617 2917 4136 513
Size: 552 1552 300 1219 3074
Fragments arranged by size:
3074 1552 1219 552 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2501 2930 3158 4724 4741 4894 5079
Size: 1255 921 429 228 1566 17 153 185
Cuts at: 5079 5524 325
Size: 445 1498
Fragments arranged by size:
1566 1498 1255 921 445 429 228 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 2571 3779
Size: 274 80 33 51 1443 567 27 1208
Cuts at: 3779 3925 4943 6655 96
Size: 146 1018 1712 138
Fragments arranged by size:
1712 1443 1208 1018 567 274 146 138
80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2255 2287 2487 2519 2551 6657 6689 2255
Size: 32 200 32 32 4106 32 2263
Fragments arranged by size:
4106 2263 200 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6697
XbaI T'CTAG_A
Cuts at: 4033 4033
Size: 6697
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2950 2011
Size: 939 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6697
XmaI C'CCGG_G
Cuts at: 1870 4022 1870
Size: 2152 4545
XmnI GAAnn'nnTTC
Cuts at: 3447 4826 3447
Size: 1379 5318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4505 276
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI