Restriction map for
pFN21AB6193
(Circular) (Six-base) MAPSORT of: pFN21AB6193.seq Check: 7820 from: 1 to: 6691
pFN21AB6193 6691 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4501 278
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3090 3597 3615 4034 1937
Size: 1153 507 18 419 4594
Fragments arranged by size:
4594 1153 507 419 18
Acc65I G'GTAC_C
Cuts at: 4012 4012
Size: 6691
AclI AA'CG_TT
Cuts at: 1563 4818 5191 1563
Size: 3255 373 3063
Fragments arranged by size:
3255 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2397 2793 2837 3824 3914 3953 4754
Size: 725 40 396 44 987 90 39 801
Cuts at: 4754 6138 6406 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 987 801 725 396 268 90
44 40 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6494
AflIII A'CryG_T
Cuts at: 1203 5590 6567 1203
Size: 4387 977 1327
Fragments arranged by size:
4387 1327 977
AgeI A'CCGG_T
Cuts at: 5522 5522
Size: 6691
AhdI GACnn_n'nnGTC
Cuts at: 5420 5420
Size: 6691
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6691
AlwNI CAG_nnn'CTG
Cuts at: 2048 2206 3745 3862 4434 6006 2048
Size: 158 1539 117 572 1572 2733
Fragments arranged by size:
2733 1572 1539 572 158 117
ApaLI G'TGCA_C
Cuts at: 4748 5904 4748
Size: 1156 5535
ApoI r'AATT_y
Cuts at: 1493 3285 3308 3475 4000 4226 4378 6487 1493
Size: 1792 23 167 525 226 152 2109 1697
Fragments arranged by size:
2109 1792 1697 525 226 167 152 23
AseI AT'TA_AT
Cuts at: 160 1032 2433 3141 5245 160
Size: 872 1401 708 2104 1606
Fragments arranged by size:
2104 1606 1401 872 708
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 2245 2508 2714 4016 1870
Size: 73 302 263 206 1302 4545
Fragments arranged by size:
4545 1302 302 263 206 73
BamHI G'GATC_C
Cuts at: 4021 4388 4021
Size: 367 6324
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3966 4012 5467 618
Size: 325 217 627 2179 46 1455 1842
Fragments arranged by size:
2179 1842 1455 627 325 217 46
BanII G_rGCy'C
Cuts at: 1391 2188 2246 2258 2997 4010 1391
Size: 797 58 12 739 1013 4072
Fragments arranged by size:
4072 1013 797 739 58 12
BbeI G_GCGC'C
Cuts at: 3970 3970
Size: 6691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2781 3108 3207 3531 928
Size: 971 882 327 99 324 4088
Fragments arranged by size:
4088 971 882 327 324 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2287 2321 4882 4916 1903
Size: 34 350 34 2561 34 3678
Fragments arranged by size:
3678 2561 350 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4583 5793 4583
Size: 1210 5481
BclI T'GATC_A
Cuts at: 1548 3798 1548
Size: 2250 4441
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5302 136
Size: 107 122 71 631 605 3630 1525
Fragments arranged by size:
3630 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2904 3732 6686 2904
Size: 828 2954 2909
Fragments arranged by size:
2954 2909 828
BlpI GC'TnA_GC
Cuts at: 2873 4108 2873
Size: 1235 5456
Bme1580I G_kGCm'C
Cuts at: 1790 4752 5908 1790
Size: 2962 1156 2573
Fragments arranged by size:
2962 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2791 1318
Size: 1473 5218
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2652 3973 4422
Size: 416 53 83 399 24 1235 1321 449
Cuts at: 4422 5380 442
Size: 958 1753
Fragments arranged by size:
1753 1321 1235 958 449 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6691
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 6691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2516 2963 3766 3803 5351 1239
Size: 1277 447 803 37 1548 2579
Fragments arranged by size:
2579 1548 1277 803 447 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4816 5681 5979 4816
Size: 865 298 5528
Fragments arranged by size:
5528 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5354 882
Size: 617 3855 2219
Fragments arranged by size:
3855 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4482 493
Size: 711 3278 2702
Fragments arranged by size:
3278 2702 711
BsaBI GATnn'nnATC
Cuts at: 6685 6685
Size: 6691
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3967 4498 4880 5515
Size: 53 83 186 936 2434 531 382 635
Cuts at: 5515 275
Size: 1451
Fragments arranged by size:
2434 1451 936 635 531 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3097 5124 5522 5796 5943 6354 3097
Size: 2027 398 274 147 411 3434
Fragments arranged by size:
3434 2027 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 1178
Size: 30 165 30 855 30 5581
Fragments arranged by size:
5581 855 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 3083 3743 1186
Size: 970 927 660 4134
Fragments arranged by size:
4134 970 927 660
BseYI C'CCAG_C
Cuts at: 5894 6390 5894
Size: 496 6195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2149 1163
Size: 573 413 5705
Fragments arranged by size:
5705 573 413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4141 4902 5930 1540
Size: 275 186 2140 761 1028 2301
Fragments arranged by size:
2301 2140 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2246 2258 2997 4752 4837 5908 2246
Size: 12 739 1755 85 1071 3029
Fragments arranged by size:
3029 1755 1071 739 85 12
BsiWI C'GTAC_G
Cuts at: 3825 3825
Size: 6691
BsmI GAATG_Cn'
Cuts at: 2452 4202 4295 2452
Size: 1750 93 4848
Fragments arranged by size:
4848 1750 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2246 2258 2997 4010 4752 4837
Size: 399 398 58 12 739 1013 742 85
Cuts at: 4837 5908 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1013 742 739 399 398 85
58 12
BspEI T'CCGG_A
Cuts at: 6354 6354
Size: 6691
BspHI T'CATG_A
Cuts at: 2479 4580 6310 2479
Size: 2101 1730 2860
Fragments arranged by size:
2860 2101 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4046 844
Size: 3202 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3901 3940 4578 1394
Size: 429 2078 39 638 3507
Fragments arranged by size:
3507 2078 638 429 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 2670 3626 5186 5360 65
Size: 1183 1422 956 1560 174 1396
Fragments arranged by size:
1560 1422 1396 1183 956 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3406 3969 5335 5522 1700
Size: 110 1596 563 1366 187 2869
Fragments arranged by size:
2869 1596 1366 563 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6691
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 2804 3230 4751 5763 2804
Size: 426 1521 1012 3732
Fragments arranged by size:
3732 1521 1012 426
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3862 794
Size: 3068 3623
BstBI TT'CG_AA
Cuts at: 5495 5495
Size: 6691
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6691
BstXI CCAn_nnnn'nTGG
Cuts at: 2665 2665
Size: 6691
BstYI r'GATC_y
Cuts at: 1972 2521 2859 2904 2935 3732 4021 4388 4773
Size: 549 338 45 31 797 289 367 385
Cuts at: 4773 4790 6242 6328 6340 6686 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 797 549 385 367 346 338
289 86 45 31 17 12
BstZ17I GTA'TAC
Cuts at: 3616 3616
Size: 6691
BtgI C'CryG_G
Cuts at: 513 1065 2004 2958 3723 513
Size: 552 939 954 765 3481
Fragments arranged by size:
3481 954 939 765 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2011 2206 2437 487
Size: 683 56 785 195 231 4741
Fragments arranged by size:
4741 785 683 231 195 56
BtsI GCAGTG_nn'
Cuts at: 3162 4207 5001 5021 3162
Size: 1045 794 20 4832
Fragments arranged by size:
4832 1045 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6656
DraI TTT'AAA
Cuts at: 3995 3995
Size: 6691
DraIII CAC_nnn'GTG
Cuts at: 3430 3430
Size: 6691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5698 809
Size: 507 4382 1802
Fragments arranged by size:
4382 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3829 4138 5027
Size: 54 1425 50 275 18 1999 309 889
Cuts at: 5027 6656 8
Size: 1629 43
Fragments arranged by size:
1999 1629 1425 889 309 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4138 1537
Size: 275 2326 4090
Fragments arranged by size:
4090 2326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3396 3477 3724 4621 1353
Size: 2043 81 247 897 3423
Fragments arranged by size:
3423 2043 897 247 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3557 5278 5652 5798 387
Size: 1064 138 1968 1721 374 146 1280
Fragments arranged by size:
1968 1721 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2244 2256 2995 2244
Size: 12 739 5940
Fragments arranged by size:
5940 739 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2397 2516 2793 2837 2963 3766
Size: 393 725 40 119 277 44 126 803
Cuts at: 3766 3803 3824 3914 3953 4754 5351 6138 6406
Size: 37 21 90 39 801 597 787 268
Cuts at: 6406 1239
Size: 1524
Fragments arranged by size:
1524 803 801 787 725 597 393 277
268 126 119 90 44 40 39 37
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 2819
Size: 6691
EcoO109I rG'GnC_Cy
Cuts at: 2203 2203
Size: 6691
EcoRI G'AATT_C
Cuts at: 4000 4378 4000
Size: 378 6313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 6691
FspI TGC'GCA
Cuts at: 5197 5197
Size: 6691
HaeII r_GCGC'y
Cuts at: 1678 3970 5838 1678
Size: 2292 1868 2531
Fragments arranged by size:
2531 2292 1868
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2258 2260 2290 2358
Size: 32 135 32 656 30 2 30 68
Cuts at: 2358 2390 2889 2921 3739 3771 3946 3978 5332
Size: 32 499 32 818 32 175 32 1354
Cuts at: 5332 5364 5406 5438 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1354 818 656 499 175 135 68
42 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3091 4035 4281 669
Size: 988 281 1153 944 246 3079
Fragments arranged by size:
3079 1153 988 944 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6691
HpaI GTT'AAC
Cuts at: 4281 4281
Size: 6691
KasI G'GCGC_C
Cuts at: 3966 3966
Size: 6691
KpnI G_GTAC'C
Cuts at: 4016 4016
Size: 6691
MfeI C'AATT_G
Cuts at: 4290 4290
Size: 6691
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 3250 5805 5989 1305
Size: 939 1006 2555 184 2007
Fragments arranged by size:
2555 2007 1006 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4869
Fragments arranged by size:
4869 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4649 5008 5167 518
Size: 648 3483 359 159 2042
Fragments arranged by size:
3483 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2301 2323 2872 3885 4107 4784 5932
Size: 439 256 22 549 1013 222 677 1148
Cuts at: 5932 6177 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1013 677 549 439 256 245
222 22
NaeI GCC'GGC
Cuts at: 3408 3971 3408
Size: 563 6128
NarI GG'CG_CC
Cuts at: 3967 3967
Size: 6691
NcoI C'CATG_G
Cuts at: 513 1065 2004 3723 513
Size: 552 939 1719 3481
Fragments arranged by size:
3481 1719 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6691
NgoMIV G'CCGG_C
Cuts at: 3406 3969 3406
Size: 563 6128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6691
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 4049 5594 1132
Size: 97 2820 1545 2229
Fragments arranged by size:
2820 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6691
PciI A'CATG_T
Cuts at: 5590 5590
Size: 6691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2707 1108
Size: 1599 5092
PfoI T'CCnGG_A
Cuts at: 2517 2862 2517
Size: 345 6346
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4760 4792 6273 6305 4760
Size: 32 1481 32 5146
Fragments arranged by size:
5146 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 2203
Size: 6691
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 4261 4261
Size: 6691
PstI C_TGCA'G
Cuts at: 830 2895 3858 3867 4043 830
Size: 2065 963 9 176 3478
Fragments arranged by size:
3478 2065 963 176 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
PvuII CAG'CTG
Cuts at: 2045 2872 3885 2045
Size: 827 1013 4851
Fragments arranged by size:
4851 1013 827
SacI G_AGCT'C
Cuts at: 2246 2258 2997 2246
Size: 12 739 5940
Fragments arranged by size:
5940 739 12
SalI G'TCGA_C
Cuts at: 1936 3089 4033 1936
Size: 1153 944 4594
Fragments arranged by size:
4594 1153 944
SapI GCTCTTCn'nnn_
Cuts at: 3396 3396
Size: 6691
SbfI CC_TGCA'GG
Cuts at: 3867 4043 3867
Size: 176 6515
ScaI AGT'ACT
Cuts at: 4939 6651 4939
Size: 1712 4979
SexAI A'CCwGG_T
Cuts at: 3609 3609
Size: 6691
SfcI C'TryA_G
Cuts at: 826 1045 2891 3189 3854 3863 3880 4039 5174
Size: 219 1846 298 665 9 17 159 1135
Cuts at: 5174 5855 6046 826
Size: 681 191 1471
Fragments arranged by size:
1846 1471 1135 681 665 298 219 191
159 17 9
SfoI GGC'GCC
Cuts at: 3968 3968
Size: 6691
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 4018 1872
Size: 2146 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4795 5696 5958 820
Size: 197 926 2852 901 262 1553
Fragments arranged by size:
2852 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6691
SphI G_CATG'C
Cuts at: 1132 4049 1132
Size: 2917 3774
SspI AAT'ATT
Cuts at: 5 52 4615 5
Size: 47 4563 2081
Fragments arranged by size:
4563 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6691
StyI C'CwwG_G
Cuts at: 513 1065 2004 3723 4130 513
Size: 552 939 1719 407 3074
Fragments arranged by size:
3074 1719 939 552 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2937 4718 4735 4888 5073 5518 325
Size: 1255 1357 1781 17 153 185 445 1498
Fragments arranged by size:
1781 1498 1357 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 3248 4937
Size: 274 80 33 51 1443 639 632 1689
Cuts at: 4937 6649 96
Size: 1712 138
Fragments arranged by size:
1712 1689 1443 639 632 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6651 6683 6651
Size: 32 6659
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6691
XbaI T'CTAG_A
Cuts at: 4027 4027
Size: 6691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2560 2320
Size: 240 6451
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6691
XmaI C'CCGG_G
Cuts at: 1870 4016 1870
Size: 2146 4545
XmnI GAAnn'nnTTC
Cuts at: 2906 3204 4820 2906
Size: 298 1616 4777
Fragments arranged by size:
4777 1616 298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4499 276
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsmBI Bsu36I EcoRV FseI FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SrfI SwaI