Restriction map for
pFN21AB6192
(Circular) (Six-base) MAPSORT of: pFN21AB6192.seq Check: 4139 from: 1 to: 6823
pFN21AB6192 6823 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2139 4166 1937
Size: 202 2027 4594
Fragments arranged by size:
4594 2027 202
Acc65I G'GTAC_C
Cuts at: 4144 4144
Size: 6823
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2762 2802 2967 3055 3139 3223 3391 3475
Size: 1130 40 165 88 84 84 168 84
Cuts at: 3475 3559 4063 4886 6270 6538 1632
Size: 84 504 823 1384 268 1917
Fragments arranged by size:
1917 1384 1130 823 504 268 168 165
88 84 84 84 84 40
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6626
AflIII A'CryG_T
Cuts at: 1203 2355 2432 5722 6699 1203
Size: 1152 77 3290 977 1327
Fragments arranged by size:
3290 1327 1152 977 77
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 5552 5552
Size: 6823
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6823
AlwNI CAG_nnn'CTG
Cuts at: 2724 4566 6138 2724
Size: 1842 1572 3409
Fragments arranged by size:
3409 1842 1572
ApaI G_GGCC'C
Cuts at: 2060 2269 2060
Size: 209 6614
ApaLI G'TGCA_C
Cuts at: 4880 6036 4880
Size: 1156 5667
ApoI r'AATT_y
Cuts at: 1493 2815 2852 2936 3021 3609 3737 3821 4132
Size: 1322 37 84 85 588 128 84 311
Cuts at: 4132 4358 4510 6619 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 588 311 226 152 128
85 84 84 37
AseI AT'TA_AT
Cuts at: 160 1032 5377 160
Size: 872 4345 1606
Fragments arranged by size:
4345 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 4148 1870
Size: 73 2205 4545
Fragments arranged by size:
4545 2205 73
BamHI G'GATC_C
Cuts at: 2310 4153 4520 2310
Size: 1843 367 4613
Fragments arranged by size:
4613 1843 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 4020 4144 5599 618
Size: 325 217 627 397 1836 124 1455 1842
Fragments arranged by size:
1842 1836 1455 627 397 325 217 124
BanII G_rGCy'C
Cuts at: 1391 2060 2166 2269 2552 2750 4142 1391
Size: 669 106 103 283 198 1392 4072
Fragments arranged by size:
4072 1392 669 283 198 106 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5852
BbvCI CC'TCA_GC
Cuts at: 2921 2921
Size: 6823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5014 5048 1903
Size: 34 3077 34 3678
Fragments arranged by size:
3678 3077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4715 5925 4715
Size: 1210 5613
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6823
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5434 136
Size: 107 122 71 631 605 3762 1525
Fragments arranged by size:
3762 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4004 6818 4004
Size: 2814 4009
BlpI GC'TnA_GC
Cuts at: 2089 2394 3345 4240 2089
Size: 305 951 895 4672
Fragments arranged by size:
4672 951 895 305
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2269 4884 6040 1790
Size: 270 209 2615 1156 2573
Fragments arranged by size:
2615 2573 1156 270 209
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2871 3543 4554
Size: 416 53 83 399 24 1454 672 1011
Cuts at: 4554 5512 442
Size: 958 1753
Fragments arranged by size:
1753 1454 1011 958 672 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2769 2769
Size: 6823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 3135 3289 3303 3471 3571 3625 3639
Size: 1373 523 154 14 168 100 54 14
Cuts at: 3639 3709 3807 3877 3891 5483 1239
Size: 70 98 70 14 1592 2579
Fragments arranged by size:
2579 1592 1373 523 168 154 100 98
70 70 54 14 14 14
Bpu10I CC'TnA_GC
Cuts at: 2921 2921
Size: 6823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 4948 5813 6111 2237
Size: 2711 865 298 2949
Fragments arranged by size:
2949 2711 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5486 882
Size: 617 3987 2219
Fragments arranged by size:
3987 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4614 493
Size: 711 3410 2702
Fragments arranged by size:
3410 2702 711
BsaBI GATnn'nnATC
Cuts at: 6817 6817
Size: 6823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4630 5012 5647 275
Size: 53 83 186 936 3097 382 635 1451
Fragments arranged by size:
3097 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3365 5256 5654 5928 6075 6486 3365
Size: 1891 398 274 147 411 3702
Fragments arranged by size:
3702 1891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3317 3347 1178
Size: 30 165 30 1914 30 4654
Fragments arranged by size:
4654 1914 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2432 2615 2840 1186
Size: 982 264 183 225 5169
Fragments arranged by size:
5169 982 264 225 183
BseYI C'CCAG_C
Cuts at: 2015 6026 6522 2015
Size: 4011 496 2316
Fragments arranged by size:
4011 2316 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2737 3238 3406 3574 1163
Size: 573 1001 501 168 168 4412
Fragments arranged by size:
4412 1001 573 501 168 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4273 5034 6062 1540
Size: 275 186 2272 761 1028 2301
Fragments arranged by size:
2301 2272 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 2496 2552 2750 4884 4969 6040 2166
Size: 330 56 198 2134 85 1071 2949
Fragments arranged by size:
2949 2134 1071 330 198 85 56
BsmI GAATG_Cn'
Cuts at: 2470 3305 3473 3893 4334 4427 2470
Size: 835 168 420 441 93 4866
Fragments arranged by size:
4866 835 441 420 168 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2166 2269 2496 2552 2750 4142
Size: 399 270 106 103 227 56 198 1392
Cuts at: 4142 4884 4969 6040 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1392 1071 742 399 270 227 198
106 103 85 56
BspEI T'CCGG_A
Cuts at: 6486 6486
Size: 6823
BspHI T'CATG_A
Cuts at: 4712 6442 4712
Size: 1730 5093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4178 844
Size: 3334 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4710 1394
Size: 429 2887 3507
Fragments arranged by size:
3507 2887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2967 3730 5318 5492 65
Size: 1183 1719 763 1588 174 1396
Fragments arranged by size:
1719 1588 1396 1183 763 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5467 5654 1700
Size: 110 3657 187 2869
Fragments arranged by size:
3657 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 4883 5895 4883
Size: 1012 5811
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6823
BstBI TT'CG_AA
Cuts at: 5627 5627
Size: 6823
BstEII G'GTnAC_C
Cuts at: 1184 2116 1184
Size: 932 5891
BstYI r'GATC_y
Cuts at: 1972 2310 2323 4004 4153 4520 4905 4922 6374
Size: 338 13 1681 149 367 385 17 1452
Cuts at: 6374 6460 6472 6818 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1681 1452 385 367 346 338 149
86 17 13 12
BstZ17I GTA'TAC
Cuts at: 2140 2140
Size: 6823
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5332
Fragments arranged by size:
5332 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6084
Fragments arranged by size:
6084 683 56
BtsI GCAGTG_nn'
Cuts at: 3310 3394 3814 3898 4339 5133 5153 3310
Size: 84 420 84 441 794 20 4980
Fragments arranged by size:
4980 794 441 420 84 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2138 2173 3661 3696 3712 3747 529
Size: 35 1574 35 1488 35 16 35 3605
Fragments arranged by size:
3605 1574 1488 35 35 35 35 16
DraI TTT'AAA
Cuts at: 4127 4127
Size: 6823
DraIII CAC_nnn'GTG
Cuts at: 2200 2200
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5830 809
Size: 507 4514 1802
Fragments arranged by size:
4514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4270 5159 6788
Size: 54 1425 50 275 18 2440 889 1629
Cuts at: 6788 8
Size: 43
Fragments arranged by size:
2440 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4270 1537
Size: 275 2458 4090
Fragments arranged by size:
4090 2458 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2540 2714 2738 2778 3142 3310 3394
Size: 749 438 174 24 40 364 168 84
Cuts at: 3394 4082 4753 1353
Size: 688 671 3423
Fragments arranged by size:
3423 749 688 671 438 364 174 168
84 40 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5410 5784 5930 387
Size: 1064 138 3821 374 146 1280
Fragments arranged by size:
3821 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2164 2550 2748 2164
Size: 386 198 6239
Fragments arranged by size:
6239 386 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2612 2762 2802 2967 3055 3135 3139
Size: 393 980 150 40 165 88 80 4
Cuts at: 3139 3223 3289 3303 3391 3471 3475 3559 3571
Size: 84 66 14 88 80 4 84 12
Cuts at: 3571 3625 3639 3709 3807 3877 3891 4063 4886
Size: 54 14 70 98 70 14 172 823
Cuts at: 4886 5483 6270 6538 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 980 823 787 597 393 268 172
165 150 98 88 88 84 84 80
80 70 70 66 54 40 14 14
14 12 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2265 3963 2265
Size: 1698 5125
EcoRI G'AATT_C
Cuts at: 3021 3609 4132 4510 3021
Size: 588 523 378 5334
Fragments arranged by size:
5334 588 523 378
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 5970 1678
Size: 4292 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2816 2848 3072 3104 5464
Size: 32 135 32 1244 32 224 32 2360
Cuts at: 5464 5496 5538 5570 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2360 1244 224 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2644 4167 4413 669
Size: 988 281 706 1523 246 3079
Fragments arranged by size:
3079 1523 988 706 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 4413 4413
Size: 6823
KpnI G_GTAC'C
Cuts at: 4148 4148
Size: 6823
MfeI C'AATT_G
Cuts at: 4422 4422
Size: 6823
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3360 5937 6121 1305
Size: 2055 2577 184 2007
Fragments arranged by size:
2577 2055 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5001
Fragments arranged by size:
5001 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2797 4781 5140 5299 518
Size: 648 1631 1984 359 159 2042
Fragments arranged by size:
2042 1984 1631 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2093 2616 4239 4916 6064 6309 1606
Size: 487 523 1623 677 1148 245 2120
Fragments arranged by size:
2120 1623 1148 677 523 487 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5332
Fragments arranged by size:
5332 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6823
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6823
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 2359 2436 2445 2802 2976 4181 5726
Size: 97 1130 77 9 357 174 1205 1545
Cuts at: 5726 1132
Size: 2229
Fragments arranged by size:
2229 1545 1205 1130 357 174 97 77
9
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6823
PciI A'CATG_T
Cuts at: 2355 2432 5722 2355
Size: 77 3290 3456
Fragments arranged by size:
3456 3290 77
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2810 1108
Size: 1702 5121
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4924 6405 6437 4892
Size: 32 1481 32 5278
Fragments arranged by size:
5278 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 3801 4393 3801
Size: 592 6231
PspOMI G'GGCC_C
Cuts at: 2056 2265 2056
Size: 209 6614
PstI C_TGCA'G
Cuts at: 830 4175 830
Size: 3345 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
PvuII CAG'CTG
Cuts at: 2093 2616 2093
Size: 523 6300
SacI G_AGCT'C
Cuts at: 2166 2552 2750 2166
Size: 386 198 6239
Fragments arranged by size:
6239 386 198
SalI G'TCGA_C
Cuts at: 1936 4165 1936
Size: 2229 4594
SapI GCTCTTCn'nnn_
Cuts at: 2102 2540 2738 3142 3310 2102
Size: 438 198 404 168 5615
Fragments arranged by size:
5615 438 404 198 168
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 5071 6783 5071
Size: 1712 5111
SexAI A'CCwGG_T
Cuts at: 3927 3927
Size: 6823
SfcI C'TryA_G
Cuts at: 826 1045 2289 4171 5306 5987 6178 826
Size: 219 1244 1882 1135 681 191 1471
Fragments arranged by size:
1882 1471 1244 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 4150 1872
Size: 2278 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 4927 5828 6090 820
Size: 197 926 273 2711 901 262 1553
Fragments arranged by size:
2711 1553 926 901 273 262 197
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 4181 1132
Size: 3049 3774
SspI AAT'ATT
Cuts at: 5 52 2957 4747 5
Size: 47 2905 1790 2081
Fragments arranged by size:
2905 2081 1790 47
StuI AGG'CCT
Cuts at: 1517 2900 3124 3544 3712 3880 1517
Size: 1383 224 420 168 168 4460
Fragments arranged by size:
4460 1383 420 224 168 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 2839 4262 513
Size: 552 939 835 1423 3074
Fragments arranged by size:
3074 1423 939 835 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4850 4867 5020 5205 5650 325
Size: 1255 3270 17 153 185 445 1498
Fragments arranged by size:
3270 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3526 5069 6781
Size: 274 80 33 51 1443 1549 1543 1712
Cuts at: 6781 96
Size: 138
Fragments arranged by size:
1712 1549 1543 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6783 6815 6783
Size: 32 6791
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6823
XbaI T'CTAG_A
Cuts at: 4159 4159
Size: 6823
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 4148 1870
Size: 2278 4545
XmnI GAAnn'nnTTC
Cuts at: 2576 2708 2984 3068 3572 4076 4952 2576
Size: 132 276 84 504 504 876 4447
Fragments arranged by size:
4447 876 504 504 276 132 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI XcmI