Restriction map for
pFN21AB6188
(Circular) (Six-base) MAPSORT of: pFN21AB6188.seq Check: 7417 from: 1 to: 6814
pFN21AB6188 6814 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3816 3816
Size: 6814
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4624 278
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4157 1937
Size: 2220 4594
Acc65I G'GTAC_C
Cuts at: 4135 4135
Size: 6814
AclI AA'CG_TT
Cuts at: 1563 2454 4941 5314 1563
Size: 891 2487 373 3063
Fragments arranged by size:
3063 2487 891 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 2604 2901 2982 3002 3249 4877 6261
Size: 611 361 297 81 20 247 1628 1384
Cuts at: 6261 6529 1632
Size: 268 1917
Fragments arranged by size:
1917 1628 1384 611 361 297 268 247
81 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6617
AflIII A'CryG_T
Cuts at: 1203 2045 5713 6690 1203
Size: 842 3668 977 1327
Fragments arranged by size:
3668 1327 977 842
AgeI A'CCGG_T
Cuts at: 5645 5645
Size: 6814
AhdI GACnn_n'nnGTC
Cuts at: 5543 5543
Size: 6814
AleI CACnn'nnGTG
Cuts at: 1166 3962 1166
Size: 2796 4018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2729 1184
Size: 1545 5269
AlwNI CAG_nnn'CTG
Cuts at: 3061 4058 4557 6129 3061
Size: 997 499 1572 3746
Fragments arranged by size:
3746 1572 997 499
ApaI G_GGCC'C
Cuts at: 2684 3386 3607 3910 2684
Size: 702 221 303 5588
Fragments arranged by size:
5588 702 303 221
ApaLI G'TGCA_C
Cuts at: 4871 6027 4871
Size: 1156 5658
ApoI r'AATT_y
Cuts at: 1493 4123 4349 4501 6610 1493
Size: 2630 226 152 2109 1697
Fragments arranged by size:
2630 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5368 160
Size: 872 4336 1606
Fragments arranged by size:
4336 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
AvaI C'yCGr_G
Cuts at: 1870 1943 3910 4139 1870
Size: 73 1967 229 4545
Fragments arranged by size:
4545 1967 229 73
BamHI G'GATC_C
Cuts at: 4144 4511 4144
Size: 367 6447
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2392 3452 4135 5590 618
Size: 325 217 627 605 1060 683 1455 1842
Fragments arranged by size:
1842 1455 1060 683 627 605 325 217
BanII G_rGCy'C
Cuts at: 1391 2226 2684 3168 3386 3396 3607 3910 4133
Size: 835 458 484 218 10 211 303 223
Cuts at: 4133 1391
Size: 4072
Fragments arranged by size:
4072 835 484 458 303 223 218 211
10
BbeI G_GCGC'C
Cuts at: 3456 3456
Size: 6814
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2384 2606 3202 3777 928
Size: 971 485 222 596 575 3965
Fragments arranged by size:
3965 971 596 575 485 222
BbvCI CC'TCA_GC
Cuts at: 2303 2303
Size: 6814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3698 3732 5005 5039 1903
Size: 34 1761 34 1273 34 3678
Fragments arranged by size:
3678 1761 1273 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4706 5916 4706
Size: 1210 5604
BclI T'GATC_A
Cuts at: 1548 3000 1548
Size: 1452 5362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2884 3149 4070
Size: 107 122 71 631 605 1212 265 921
Cuts at: 4070 4079 5425 136
Size: 9 1346 1525
Fragments arranged by size:
1525 1346 1212 921 631 605 265 122
107 71 9
BglII A'GATC_T
Cuts at: 2254 6809 2254
Size: 4555 2259
BlpI GC'TnA_GC
Cuts at: 2024 2069 4231 2024
Size: 45 2162 4607
Fragments arranged by size:
4607 2162 45
Bme1580I G_kGCm'C
Cuts at: 1790 2684 3386 3607 3910 3993 4875 6031 1790
Size: 894 702 221 303 83 882 1156 2573
Fragments arranged by size:
2573 1156 894 882 702 303 221 83
BmgBI CAC'GTC
Cuts at: 1318 2197 1318
Size: 879 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2092 2828 2837
Size: 416 53 83 399 24 675 736 9
Cuts at: 2837 3414 4545 5503 442
Size: 577 1131 958 1753
Fragments arranged by size:
1753 1131 958 736 675 577 416 399
83 53 24 9
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2545 3083 3158 3350 3630 5474 1239
Size: 1306 538 75 192 280 1844 2579
Fragments arranged by size:
2579 1844 1306 538 280 192 75
Bpu10I CC'TnA_GC
Cuts at: 2303 2303
Size: 6814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3152 4939 5804 6102 3152
Size: 1787 865 298 3864
Fragments arranged by size:
3864 1787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3468 3881 5477 882
Size: 617 1969 413 1596 2219
Fragments arranged by size:
2219 1969 1596 617 413
BsaAI yAC'GTr
Cuts at: 493 1204 4605 493
Size: 711 3401 2702
Fragments arranged by size:
3401 2702 711
BsaBI GATnn'nnATC
Cuts at: 3935 3971 6808 3935
Size: 36 2837 3941
Fragments arranged by size:
3941 2837 36
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3453 4621 5003 5638
Size: 53 83 186 936 1920 1168 382 635
Cuts at: 5638 275
Size: 1451
Fragments arranged by size:
1920 1451 1168 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5247 5645 5919 6066 6477 5247
Size: 398 274 147 411 5584
Fragments arranged by size:
5584 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 2391 2421 2571
Size: 30 165 30 768 30 190 30 150
Cuts at: 2571 2601 3824 3854 4067 4097 1178
Size: 30 1223 30 213 30 3895
Fragments arranged by size:
3895 1223 768 213 190 165 150 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2287 2548 2597 2689 3270 3487 3938
Size: 961 140 261 49 92 581 217 451
Cuts at: 3938 1186
Size: 4062
Fragments arranged by size:
4062 961 581 451 261 217 140 92
49
BseYI C'CCAG_C
Cuts at: 2644 2792 3322 3396 3401 3558 3817 6017 6513
Size: 148 530 74 5 157 259 2200 496
Cuts at: 6513 2644
Size: 2945
Fragments arranged by size:
2945 2200 530 496 259 157 148 74
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6241
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4264 5025 6053 1540
Size: 275 186 2263 761 1028 2301
Fragments arranged by size:
2301 2263 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 3168 4875 4960 6031 2370
Size: 798 1707 85 1071 3153
Fragments arranged by size:
3153 1707 1071 798 85
BsmI GAATG_Cn'
Cuts at: 3121 3743 4325 4418 3121
Size: 622 582 93 5517
Fragments arranged by size:
5517 622 582 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2327 2327
Size: 6814
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2370 2684 3168 3386 3396 3607
Size: 399 436 144 314 484 218 10 211
Cuts at: 3607 3910 3993 4133 4875 4960 6031 1391
Size: 303 83 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 436 399 314 303
218 211 144 140 85 83 10
BspEI T'CCGG_A
Cuts at: 6477 6477
Size: 6814
BspHI T'CATG_A
Cuts at: 4703 6433 4703
Size: 1730 5084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 3816 4169 844
Size: 1229 1743 353 3489
Fragments arranged by size:
3489 1743 1229 353
BsrBI CCG'CTC
Cuts at: 1394 1823 2933 4701 1394
Size: 429 1110 1768 3507
Fragments arranged by size:
3507 1768 1110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3222 4036 5309 5483 65
Size: 1183 1974 814 1273 174 1396
Fragments arranged by size:
1974 1396 1273 1183 814 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2887 3507 5458 5645 1700
Size: 110 1077 620 1951 187 2869
Fragments arranged by size:
2869 1951 1077 620 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6814
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6814
BssSI C'ACGA_G
Cuts at: 4874 5886 4874
Size: 1012 5802
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6814
BstBI TT'CG_AA
Cuts at: 5618 5618
Size: 6814
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6814
BstXI CCAn_nnnn'nTGG
Cuts at: 3674 3674
Size: 6814
BstYI r'GATC_y
Cuts at: 1972 2254 3591 4144 4511 4896 4913 6365 6451
Size: 282 1337 553 367 385 17 1452 86
Cuts at: 6451 6463 6809 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1337 553 385 367 346 282
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2701 3387 3533 2701
Size: 686 146 5982
Fragments arranged by size:
5982 686 146
BtgI C'CryG_G
Cuts at: 513 1065 2004 2228 2903 3629 3873 3901 513
Size: 552 939 224 675 726 244 28 3426
Fragments arranged by size:
3426 939 726 675 552 244 224 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2301 487
Size: 683 56 1075 5000
Fragments arranged by size:
5000 1075 683 56
BtsI GCAGTG_nn'
Cuts at: 2732 4330 5124 5144 2732
Size: 1598 794 20 4402
Fragments arranged by size:
4402 1598 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6779
DraI TTT'AAA
Cuts at: 4118 4118
Size: 6814
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5821 809
Size: 507 4505 1802
Fragments arranged by size:
4505 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 4261 5150
Size: 54 1425 50 275 18 297 2134 889
Cuts at: 5150 6779 8
Size: 1629 43
Fragments arranged by size:
2134 1629 1425 889 297 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4261 1537
Size: 275 2449 4090
Fragments arranged by size:
4090 2449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2153 2296 2912 3224 3494 4744 1353
Size: 800 143 616 312 270 1250 3423
Fragments arranged by size:
3423 1250 800 616 312 270 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3095 5401 5775 5921 387
Size: 1064 138 435 1071 2306 374 146 1280
Fragments arranged by size:
2306 1280 1071 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3166 3166
Size: 6814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2545 2604 2901 2982 3002 3083
Size: 393 611 302 59 297 81 20 81
Cuts at: 3083 3158 3249 3350 3630 4877 5474 6261 6529
Size: 75 91 101 280 1247 597 787 268
Cuts at: 6529 1239
Size: 1524
Fragments arranged by size:
1524 1247 787 611 597 393 302 297
280 268 101 91 81 81 75 59
20
EcoNI CCTnn'n_nnAGG
Cuts at: 2855 2855
Size: 6814
EcoO109I rG'GnC_Cy
Cuts at: 2587 3142 3372 3382 3383 3603 3906 3907 2587
Size: 555 230 10 1 220 303 1 5494
Fragments arranged by size:
5494 555 303 230 220 10 1 1
EcoRI G'AATT_C
Cuts at: 4123 4501 4123
Size: 378 6436
FspI TGC'GCA
Cuts at: 5320 5320
Size: 6814
HaeII r_GCGC'y
Cuts at: 1678 3456 3571 5961 1678
Size: 1778 115 2390 2531
Fragments arranged by size:
2531 2390 1778 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2411 2443 2462
Size: 32 135 32 599 32 208 32 19
Cuts at: 2462 2494 2571 2597 2603 2629 2684 2716 3878
Size: 32 77 26 6 26 55 32 1162
Cuts at: 3878 3910 5455 5487 5529 5561 1373
Size: 32 1545 32 42 32 2626
Fragments arranged by size:
2626 1545 1162 599 208 135 77 55
42 32 32 32 32 32 32 32
32 32 26 26 19 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 4158 4404 669
Size: 988 281 589 1631 246 3079
Fragments arranged by size:
3079 1631 988 589 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6814
HpaI GTT'AAC
Cuts at: 4404 4404
Size: 6814
KasI G'GCGC_C
Cuts at: 3452 3452
Size: 6814
KpnI G_GTAC'C
Cuts at: 4139 4139
Size: 6814
MfeI C'AATT_G
Cuts at: 3756 4413 3756
Size: 657 6157
MluI A'CGCG_T
Cuts at: 2045 6690 2045
Size: 4645 2169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3691 5928 6112 1305
Size: 2386 2237 184 2007
Fragments arranged by size:
2386 2237 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4992
Fragments arranged by size:
4992 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3962 4772 5131 5290 518
Size: 648 2796 810 359 159 2042
Fragments arranged by size:
2796 2042 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3706 4230 4907 6055 6300 1606
Size: 2100 524 677 1148 245 2120
Fragments arranged by size:
2120 2100 1148 677 524 245
NaeI GCC'GGC
Cuts at: 2889 3509 2889
Size: 620 6194
NarI GG'CG_CC
Cuts at: 3453 3453
Size: 6814
NcoI C'CATG_G
Cuts at: 513 1065 2004 2228 3873 513
Size: 552 939 224 1645 3454
Fragments arranged by size:
3454 1645 939 552 224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6814
NgoMIV G'CCGG_C
Cuts at: 2887 3507 2887
Size: 620 6194
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6814
NotI GC'GGCC_GC
Cuts at: 4261 4261
Size: 6814
NspI r_CATG'y
Cuts at: 1132 1229 4172 5717 1132
Size: 97 2943 1545 2229
Fragments arranged by size:
2943 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3379 3446 1348
Size: 2031 67 4716
Fragments arranged by size:
4716 2031 67
PciI A'CATG_T
Cuts at: 5713 5713
Size: 6814
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6814
PfoI T'CCnGG_A
Cuts at: 2589 3029 2589
Size: 440 6374
PmeI GTTT'AAAC
Cuts at: 4118 4118
Size: 6814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4883 4915 6396 6428 4883
Size: 32 1481 32 5269
Fragments arranged by size:
5269 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 3372 2587
Size: 785 6029
PshAI GACnn'nnGTC
Cuts at: 1613 2085 3619 1613
Size: 472 1534 4808
Fragments arranged by size:
4808 1534 472
PsiI TTA'TAA
Cuts at: 4384 4384
Size: 6814
PspOMI G'GGCC_C
Cuts at: 2680 3382 3603 3906 2680
Size: 702 221 303 5588
Fragments arranged by size:
5588 702 303 221
PstI C_TGCA'G
Cuts at: 830 2094 2739 2814 3057 4063 4166 830
Size: 1264 645 75 243 1006 103 3478
Fragments arranged by size:
3478 1264 1006 645 243 103 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6814
PvuII CAG'CTG
Cuts at: 3706 3706
Size: 6814
SacI G_AGCT'C
Cuts at: 3168 3168
Size: 6814
SalI G'TCGA_C
Cuts at: 1936 4156 1936
Size: 2220 4594
SapI GCTCTTCn'nnn_
Cuts at: 2153 2296 2912 3494 2153
Size: 143 616 582 5473
Fragments arranged by size:
5473 616 582 143
SbfI CC_TGCA'GG
Cuts at: 4166 4166
Size: 6814
ScaI AGT'ACT
Cuts at: 3084 5062 6774 3084
Size: 1978 1712 3124
Fragments arranged by size:
3124 1978 1712
SfcI C'TryA_G
Cuts at: 826 1045 2090 2735 2748 2810 3053 4059 4162
Size: 219 1045 645 13 62 243 1006 103
Cuts at: 4162 5297 5978 6169 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 1006 681 645 243 219
191 103 62 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 4079 4079
Size: 6814
SfoI GGC'GCC
Cuts at: 3454 3454
Size: 6814
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6814
SmaI CCC'GGG
Cuts at: 1872 3912 4141 1872
Size: 2040 229 4545
Fragments arranged by size:
4545 2040 229
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3167 4918 5819 6081 820
Size: 197 926 1224 1751 901 262 1553
Fragments arranged by size:
1751 1553 1224 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4605 493
Size: 4112 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6814
SphI G_CATG'C
Cuts at: 1132 4172 1132
Size: 3040 3774
SspI AAT'ATT
Cuts at: 5 52 4738 5
Size: 47 4686 2081
Fragments arranged by size:
4686 2081 47
StuI AGG'CCT
Cuts at: 1517 2537 4082 1517
Size: 1020 1545 4249
Fragments arranged by size:
4249 1545 1020
StyI C'CwwG_G
Cuts at: 513 1065 2004 2228 2388 3456 3873 4253 513
Size: 552 939 224 160 1068 417 380 3074
Fragments arranged by size:
3074 1068 939 552 417 380 224 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3738 3777 4841 4858 5011 5196 5641
Size: 1255 2158 39 1064 17 153 185 445
Cuts at: 5641 325
Size: 1498
Fragments arranged by size:
2158 1498 1255 1064 445 185 153 39
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3082 5060 6772
Size: 274 80 33 51 1443 1105 1978 1712
Cuts at: 6772 96
Size: 138
Fragments arranged by size:
1978 1712 1443 1105 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2391 2423 4067 4099 6774 6806 2391
Size: 32 1644 32 2675 32 2399
Fragments arranged by size:
2675 2399 1644 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2383 2605 3892 1316
Size: 1067 222 1287 4238
Fragments arranged by size:
4238 1287 1067 222
XbaI T'CTAG_A
Cuts at: 4150 4150
Size: 6814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3856 3856
Size: 6814
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6814
XmaI C'CCGG_G
Cuts at: 1870 3910 4139 1870
Size: 2040 229 4545
Fragments arranged by size:
4545 2040 229
XmnI GAAnn'nnTTC
Cuts at: 4943 4943
Size: 6814
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4622 276
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SrfI SwaI