Restriction map for
pFN21AB6187
(Circular) (Six-base) MAPSORT of: pFN21AB6187.seq Check: 2882 from: 1 to: 6685
pFN21AB6187 6685 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2992 2992
Size: 6685
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4495 278
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4028 1937
Size: 2091 4594
Acc65I G'GTAC_C
Cuts at: 3462 4006 3462
Size: 544 6141
AclI AA'CG_TT
Cuts at: 1563 4812 5185 1563
Size: 3249 373 3063
Fragments arranged by size:
3249 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3223 3944 3989 4748 6132 6400 1632
Size: 1591 721 45 759 1384 268 1917
Fragments arranged by size:
1917 1591 1384 759 721 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6488
AflIII A'CryG_T
Cuts at: 1203 2114 5584 6561 1203
Size: 911 3470 977 1327
Fragments arranged by size:
3470 1327 977 911
AgeI A'CCGG_T
Cuts at: 5516 5516
Size: 6685
AhdI GACnn_n'nnGTC
Cuts at: 5414 5414
Size: 6685
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3896 1184
Size: 2712 3973
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 2207
Size: 32 6653
AlwNI CAG_nnn'CTG
Cuts at: 2132 4428 6000 2132
Size: 2296 1572 2817
Fragments arranged by size:
2817 2296 1572
ApaLI G'TGCA_C
Cuts at: 4742 5898 4742
Size: 1156 5529
ApoI r'AATT_y
Cuts at: 1493 2915 3994 4220 4372 6481 1493
Size: 1422 1079 226 152 2109 1697
Fragments arranged by size:
2109 1697 1422 1079 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5239 160
Size: 872 4207 1606
Fragments arranged by size:
4207 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
AvaI C'yCGr_G
Cuts at: 1870 1943 2652 4010 1870
Size: 73 709 1358 4545
Fragments arranged by size:
4545 1358 709 73
BamHI G'GATC_C
Cuts at: 4015 4382 4015
Size: 367 6318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 2637 2711 3462 3579
Size: 325 217 627 471 379 74 751 117
Cuts at: 3579 4006 5461 618
Size: 427 1455 1842
Fragments arranged by size:
1842 1455 751 627 471 427 379 325
217 117 74
BanII G_rGCy'C
Cuts at: 1391 2588 4004 1391
Size: 1197 1416 4072
Fragments arranged by size:
4072 1416 1197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2397 2435 3229 928
Size: 971 498 38 794 4384
Fragments arranged by size:
4384 971 794 498 38
BbvCI CC'TCA_GC
Cuts at: 3625 3825 3625
Size: 200 6485
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4876 4910 1903
Size: 34 50 34 2855 34 3678
Fragments arranged by size:
3678 2855 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3010 4577 5787 3010
Size: 1567 1210 3908
Fragments arranged by size:
3908 1567 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6685
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3068 5296 136
Size: 107 122 71 631 605 1396 2228 1525
Fragments arranged by size:
2228 1525 1396 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6680 6680
Size: 6685
BlpI GC'TnA_GC
Cuts at: 3058 4102 3058
Size: 1044 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2263 4746 5902 1790
Size: 473 2483 1156 2573
Fragments arranged by size:
2573 2483 1156 473
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4416 5374 442
Size: 416 53 83 399 24 2999 958 1753
Fragments arranged by size:
2999 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6685
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2976 3292 2976
Size: 316 6369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2709 3374 3631 3755 5345 1239
Size: 1470 665 257 124 1590 2579
Fragments arranged by size:
2579 1590 1470 665 257 124
Bpu10I CC'TnA_GC
Cuts at: 3625 3825 3625
Size: 200 6485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3233 3926 4810 5675 5973 3233
Size: 693 884 865 298 3945
Fragments arranged by size:
3945 884 865 693 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5348 882
Size: 617 3849 2219
Fragments arranged by size:
3849 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4476 493
Size: 711 3272 2702
Fragments arranged by size:
3272 2702 711
BsaBI GATnn'nnATC
Cuts at: 6679 6679
Size: 6685
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4492 4874 5509 275
Size: 53 83 186 936 2959 382 635 1451
Fragments arranged by size:
2959 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3013 5118 5516 5790 5937 6348 3013
Size: 2105 398 274 147 411 3350
Fragments arranged by size:
3350 2105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2989 3019 1178
Size: 30 165 30 1586 30 4844
Fragments arranged by size:
4844 1586 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3766 1186
Size: 2580 4105
BseYI C'CCAG_C
Cuts at: 2125 2560 2771 3053 3316 3928 5888 6384 2125
Size: 435 211 282 263 612 1960 496 2426
Fragments arranged by size:
2426 1960 612 496 435 282 263 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3921 1163
Size: 573 2185 3927
Fragments arranged by size:
3927 2185 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4135 4896 5924 1540
Size: 275 186 2134 761 1028 2301
Fragments arranged by size:
2301 2134 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4746 4831 5902 4746
Size: 85 1071 5529
Fragments arranged by size:
5529 1071 85
BsmI GAATG_Cn'
Cuts at: 2404 4196 4289 2404
Size: 1792 93 4800
Fragments arranged by size:
4800 1792 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 2588 4004 4746 4831 5902 1391
Size: 399 473 325 1416 742 85 1071 2174
Fragments arranged by size:
2174 1416 1071 742 473 399 325 85
BspEI T'CCGG_A
Cuts at: 6348 6348
Size: 6685
BspHI T'CATG_A
Cuts at: 4574 6304 4574
Size: 1730 4955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2706 2992 4040 844
Size: 1862 286 1048 3489
Fragments arranged by size:
3489 1862 1048 286
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 4572 1394
Size: 429 1684 1065 3507
Fragments arranged by size:
3507 1684 1065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 5180 5354 65
Size: 1183 889 3043 174 1396
Fragments arranged by size:
3043 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3582 3963 5329 5516 1700
Size: 110 1772 381 1366 187 2869
Fragments arranged by size:
2869 1772 1366 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6685
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6685
BssSI C'ACGA_G
Cuts at: 3860 4745 5757 3860
Size: 885 1012 4788
Fragments arranged by size:
4788 1012 885
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3642 794
Size: 2848 3837
BstBI TT'CG_AA
Cuts at: 5489 5489
Size: 6685
BstEII G'GTnAC_C
Cuts at: 1184 2070 1184
Size: 886 5799
BstXI CCAn_nnnn'nTGG
Cuts at: 2342 2342
Size: 6685
BstYI r'GATC_y
Cuts at: 1972 4015 4382 4767 4784 6236 6322 6334 6680
Size: 2043 367 385 17 1452 86 12 346
Cuts at: 6680 1972
Size: 1977
Fragments arranged by size:
2043 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3259 513
Size: 552 2194 3939
Fragments arranged by size:
3939 2194 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2469 3759 487
Size: 683 56 1243 1290 3413
Fragments arranged by size:
3413 1290 1243 683 56
BtsI GCAGTG_nn'
Cuts at: 3429 3618 3901 4201 4995 5015 3429
Size: 189 283 300 794 20 5099
Fragments arranged by size:
5099 794 300 283 189 20
ClaI AT'CG_AT
Cuts at: 1554 3867 1554
Size: 2313 4372
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2215 2227 2250 2262 3541 3576 529
Size: 35 1651 12 23 12 1279 35 3638
Fragments arranged by size:
3638 1651 1279 35 35 23 12 12
DraI TTT'AAA
Cuts at: 3989 3989
Size: 6685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3421 5692 809
Size: 507 2105 2271 1802
Fragments arranged by size:
2271 2105 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3763 4132 5021
Size: 54 1425 50 275 18 1933 369 889
Cuts at: 5021 6650 8
Size: 1629 43
Fragments arranged by size:
1933 1629 1425 889 369 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4132 1537
Size: 275 2320 4090
Fragments arranged by size:
4090 2320 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2921 4615 1353
Size: 1568 1694 3423
Fragments arranged by size:
3423 1694 1568
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5272 5646 5792 387
Size: 1064 138 3683 374 146 1280
Fragments arranged by size:
3683 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2709 3223 3374 3631 3755 3944 3989
Size: 393 1077 514 151 257 124 189 45
Cuts at: 3989 4748 5345 6132 6400 1239
Size: 759 597 787 268 1524
Fragments arranged by size:
1524 1077 787 759 597 514 393 268
257 189 151 124 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3574 3574
Size: 6685
EcoO109I rG'GnC_Cy
Cuts at: 2047 2206 2622 2933 3070 3113 3769 3877 3932
Size: 159 416 311 137 43 656 108 55
Cuts at: 3932 2047
Size: 4800
Fragments arranged by size:
4800 656 416 311 159 137 108 55
43
EcoRI G'AATT_C
Cuts at: 3994 4372 3994
Size: 378 6307
FspI TGC'GCA
Cuts at: 3635 3893 5191 3635
Size: 258 1298 5129
Fragments arranged by size:
5129 1298 258
HaeII r_GCGC'y
Cuts at: 1678 2132 5832 1678
Size: 454 3700 2531
Fragments arranged by size:
3700 2531 454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 3527 3559 3674
Size: 32 135 32 896 32 1027 32 115
Cuts at: 3674 3706 3761 3793 5326 5358 5400 5432 1373
Size: 32 55 32 1533 32 42 32 2626
Fragments arranged by size:
2626 1533 1027 896 135 115 55 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 3325 4029 4275 669
Size: 988 281 382 1005 704 246 3079
Fragments arranged by size:
3079 1005 988 704 382 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6685
HpaI GTT'AAC
Cuts at: 4275 4275
Size: 6685
KpnI G_GTAC'C
Cuts at: 3466 4010 3466
Size: 544 6141
MfeI C'AATT_G
Cuts at: 4284 4284
Size: 6685
MluI A'CGCG_T
Cuts at: 6561 6561
Size: 6685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2227 3702 5799 5983 1305
Size: 922 1475 2097 184 2007
Fragments arranged by size:
2097 2007 1475 922 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4863
Fragments arranged by size:
4863 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3264 3905 4643 5002 5161 518
Size: 648 2098 641 738 359 159 2042
Fragments arranged by size:
2098 2042 738 648 641 359 159
MspA1I CmG'CkG
Cuts at: 1606 2327 2903 3629 4101 4778 5926 6171 1606
Size: 721 576 726 472 677 1148 245 2120
Fragments arranged by size:
2120 1148 726 721 677 576 472 245
NcoI C'CATG_G
Cuts at: 513 1065 3259 513
Size: 552 2194 3939
Fragments arranged by size:
3939 2194 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6685
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6685
NotI GC'GGCC_GC
Cuts at: 4132 4132
Size: 6685
NspI r_CATG'y
Cuts at: 1132 1229 2118 4043 5588 1132
Size: 97 889 1925 1545 2229
Fragments arranged by size:
2229 1925 1545 889 97
PasI CC'CwG_GG
Cuts at: 1348 3716 1348
Size: 2368 4317
PciI A'CATG_T
Cuts at: 2114 5584 2114
Size: 3470 3215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2384 1108
Size: 1276 5409
PfoI T'CCnGG_A
Cuts at: 3351 3810 3351
Size: 459 6226
PmeI GTTT'AAAC
Cuts at: 3989 3989
Size: 6685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 6267 6299 4754
Size: 32 1481 32 5140
Fragments arranged by size:
5140 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2206 2933 3769 2047
Size: 159 727 836 4963
Fragments arranged by size:
4963 836 727 159
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6685
PsiI TTA'TAA
Cuts at: 4255 4255
Size: 6685
PstI C_TGCA'G
Cuts at: 830 2703 4037 830
Size: 1873 1334 3478
Fragments arranged by size:
3478 1873 1334
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6685
PvuII CAG'CTG
Cuts at: 2327 2903 3629 2327
Size: 576 726 5383
Fragments arranged by size:
5383 726 576
RsrII CG'GwC_CG
Cuts at: 3724 3724
Size: 6685
SalI G'TCGA_C
Cuts at: 1936 4027 1936
Size: 2091 4594
SanDI GG'GwC_CC
Cuts at: 3769 3769
Size: 6685
SbfI CC_TGCA'GG
Cuts at: 4037 4037
Size: 6685
ScaI AGT'ACT
Cuts at: 4933 6645 4933
Size: 1712 4973
SexAI A'CCwGG_T
Cuts at: 3469 3469
Size: 6685
SfcI C'TryA_G
Cuts at: 826 1045 2017 2372 2699 4033 5168 5849 6040
Size: 219 972 355 327 1334 1135 681 191
Cuts at: 6040 826
Size: 1471
Fragments arranged by size:
1471 1334 1135 972 681 355 327 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6685
SmaI CCC'GGG
Cuts at: 1872 4012 1872
Size: 2140 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3248 3941 4789 5690 5952 820
Size: 197 926 1305 693 848 901 262 1553
Fragments arranged by size:
1553 1305 926 901 848 693 262 197
SnaBI TAC'GTA
Cuts at: 493 4476 493
Size: 3983 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6685
SphI G_CATG'C
Cuts at: 1132 4043 1132
Size: 2911 3774
SspI AAT'ATT
Cuts at: 5 52 4609 5
Size: 47 4557 2081
Fragments arranged by size:
4557 2081 47
StuI AGG'CCT
Cuts at: 1517 3203 1517
Size: 1686 4999
StyI C'CwwG_G
Cuts at: 513 1065 3259 3510 4124 513
Size: 552 2194 251 614 3074
Fragments arranged by size:
3074 2194 614 552 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2601 2872 3741 4712 4729 4882 5067
Size: 1255 1021 271 869 971 17 153 185
Cuts at: 5067 5512 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1021 971 869 445 271 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3441 3912 4931
Size: 274 80 33 51 1443 1464 471 1019
Cuts at: 4931 6643 96
Size: 1712 138
Fragments arranged by size:
1712 1464 1443 1019 471 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6645 6677 6645
Size: 32 6653
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6685
XbaI T'CTAG_A
Cuts at: 4021 4021
Size: 6685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2797 2797
Size: 6685
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6685
XmaI C'CCGG_G
Cuts at: 1870 4010 1870
Size: 2140 4545
XmnI GAAnn'nnTTC
Cuts at: 4814 4814
Size: 6685
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4493 276
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI SacI SacII SapI SfiI
SfoI SrfI SwaI