Restriction map for
pFN21AB6181
(Circular) (Six-base) MAPSORT of: pFN21AB6181.seq Check: 8138 from: 1 to: 6682
pFN21AB6181 6682 bp
With 182 enzymes: SgfI *
July 29, 2009 14:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 4492 278
Size: 53 83 186 936 1957 999 2468
Fragments arranged by size:
2468 1957 999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3813 4025 1937
Size: 1876 212 4594
Fragments arranged by size:
4594 1876 212
Acc65I G'GTAC_C
Cuts at: 4003 4003
Size: 6682
AclI AA'CG_TT
Cuts at: 1563 4809 5182 1563
Size: 3246 373 3063
Fragments arranged by size:
3246 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1999 3218 3650 4745 6129 6397 1632
Size: 367 1219 432 1095 1384 268 1917
Fragments arranged by size:
1917 1384 1219 1095 432 367 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6485
AflIII A'CryG_T
Cuts at: 1203 5581 6558 1203
Size: 4378 977 1327
Fragments arranged by size:
4378 1327 977
AgeI A'CCGG_T
Cuts at: 5513 5513
Size: 6682
AhdI GACnn_n'nnGTC
Cuts at: 5411 5411
Size: 6682
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6682
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6682
AlwNI CAG_nnn'CTG
Cuts at: 2215 4425 5997 2215
Size: 2210 1572 2900
Fragments arranged by size:
2900 2210 1572
ApaI G_GGCC'C
Cuts at: 2693 2693
Size: 6682
ApaLI G'TGCA_C
Cuts at: 4739 5895 4739
Size: 1156 5526
ApoI r'AATT_y
Cuts at: 1493 2588 3612 3941 3991 4217 4369 6478 1493
Size: 1095 1024 329 50 226 152 2109 1697
Fragments arranged by size:
2109 1697 1095 1024 329 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 3185 5236 160
Size: 872 2153 2051 1606
Fragments arranged by size:
2153 2051 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
AvaI C'yCGr_G
Cuts at: 1870 1943 4007 1870
Size: 73 2064 4545
Fragments arranged by size:
4545 2064 73
BamHI G'GATC_C
Cuts at: 4012 4379 4012
Size: 367 6315
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2091 4003 5458 618
Size: 325 217 627 267 37 1912 1455 1842
Fragments arranged by size:
1912 1842 1455 627 325 267 217 37
BanII G_rGCy'C
Cuts at: 1391 2686 2693 4001 1391
Size: 1295 7 1308 4072
Fragments arranged by size:
4072 1308 1295 7
BbeI G_GCGC'C
Cuts at: 2095 2095
Size: 6682
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2755 2881 928
Size: 971 856 126 4729
Fragments arranged by size:
4729 971 856 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4873 4907 1903
Size: 34 2936 34 3678
Fragments arranged by size:
3678 2936 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4574 5784 4574
Size: 1210 5472
BclI T'GATC_A
Cuts at: 1548 3331 1548
Size: 1783 4899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5293 136
Size: 107 122 71 631 605 337 3284 1525
Fragments arranged by size:
3284 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6677 6677
Size: 6682
BlpI GC'TnA_GC
Cuts at: 4099 4099
Size: 6682
Bme1580I G_kGCm'C
Cuts at: 1790 2693 4743 5899 1790
Size: 903 2050 1156 2573
Fragments arranged by size:
2573 2050 1156 903
BmgBI CAC'GTC
Cuts at: 1318 3817 1318
Size: 2499 4183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3766 4413 5371
Size: 416 53 83 399 24 2349 647 958
Cuts at: 5371 442
Size: 1753
Fragments arranged by size:
2349 1753 958 647 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2492 3994 2492
Size: 1502 5180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3315 5342 1239
Size: 2076 2027 2579
Fragments arranged by size:
2579 2076 2027
Bpu10I CC'TnA_GC
Cuts at: 2685 2685
Size: 6682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2182 3026 3093 4807 5672 5970 2182
Size: 844 67 1714 865 298 2894
Fragments arranged by size:
2894 1714 865 844 298 67
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3241 3369 3529 5345 882
Size: 617 1742 128 160 1816 2219
Fragments arranged by size:
2219 1816 1742 617 160 128
BsaAI yAC'GTr
Cuts at: 493 1204 4473 493
Size: 711 3269 2702
Fragments arranged by size:
3269 2702 711
BsaBI GATnn'nnATC
Cuts at: 3207 6676 3207
Size: 3469 3213
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2092 3490 4489 4871
Size: 53 83 186 936 559 1398 999 382
Cuts at: 4871 5506 275
Size: 635 1451
Fragments arranged by size:
1451 1398 999 936 635 559 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5115 5513 5787 5934 6345 5115
Size: 398 274 147 411 5452
Fragments arranged by size:
5452 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3255 3285 1178
Size: 30 165 30 1852 30 4575
Fragments arranged by size:
4575 1852 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2032 2510 1186
Size: 846 478 5358
Fragments arranged by size:
5358 846 478
BseYI C'CCAG_C
Cuts at: 2692 3134 3684 5885 6381 2692
Size: 442 550 2201 496 2993
Fragments arranged by size:
2993 2201 550 496 442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 3822 1163
Size: 573 357 1729 4023
Fragments arranged by size:
4023 1729 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4132 4893 5921 1540
Size: 275 186 2131 761 1028 2301
Fragments arranged by size:
2301 2131 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2686 4743 4828 5899 2686
Size: 2057 85 1071 3469
Fragments arranged by size:
3469 2057 1071 85
BsmI GAATG_Cn'
Cuts at: 3096 3797 4193 4286 3096
Size: 701 396 93 5492
Fragments arranged by size:
5492 701 396 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2686 2693 4001 4743 4828 5899 1391
Size: 399 896 7 1308 742 85 1071 2174
Fragments arranged by size:
2174 1308 1071 896 742 399 85 7
BspEI T'CCGG_A
Cuts at: 6345 6345
Size: 6682
BspHI T'CATG_A
Cuts at: 3901 4571 6301 3901
Size: 670 1730 4282
Fragments arranged by size:
4282 1730 670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3921 4037 844
Size: 3077 116 3489
Fragments arranged by size:
3489 3077 116
BsrBI CCG'CTC
Cuts at: 1394 1823 4569 1394
Size: 429 2746 3507
Fragments arranged by size:
3507 2746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5177 5351 65
Size: 1183 3929 174 1396
Fragments arranged by size:
3929 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3158 5326 5513 1700
Size: 110 1348 2168 187 2869
Fragments arranged by size:
2869 2168 1348 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6682
BssSI C'ACGA_G
Cuts at: 4742 5754 4742
Size: 1012 5670
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6682
BstBI TT'CG_AA
Cuts at: 2746 5486 2746
Size: 2740 3942
BstEII G'GTnAC_C
Cuts at: 1184 2990 1184
Size: 1806 4876
BstYI r'GATC_y
Cuts at: 1972 4012 4379 4764 4781 6233 6319 6331 6677
Size: 2040 367 385 17 1452 86 12 346
Cuts at: 6677 1972
Size: 1977
Fragments arranged by size:
2040 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5191
Fragments arranged by size:
5191 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2377 3758 487
Size: 683 56 1151 1381 3411
Fragments arranged by size:
3411 1381 1151 683 56
BtsI GCAGTG_nn'
Cuts at: 4198 4992 5012 4198
Size: 794 20 5868
Fragments arranged by size:
5868 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2968 3003 529
Size: 35 2404 35 4208
Fragments arranged by size:
4208 2404 35 35
DraI TTT'AAA
Cuts at: 3207 3986 3207
Size: 779 5903
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5689 809
Size: 507 4373 1802
Fragments arranged by size:
4373 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3156 4129 5018
Size: 54 1425 50 275 18 1326 973 889
Cuts at: 5018 6647 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1326 973 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4129 1537
Size: 275 2317 4090
Fragments arranged by size:
4090 2317 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3869 4612 1353
Size: 2516 743 3423
Fragments arranged by size:
3423 2516 743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5269 5643 5789 387
Size: 1064 138 3680 374 146 1280
Fragments arranged by size:
3680 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2684 2684
Size: 6682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 3218 3315 3650 4745 5342 6129
Size: 393 367 1219 97 335 1095 597 787
Cuts at: 6129 6397 1239
Size: 268 1524
Fragments arranged by size:
1524 1219 1095 787 597 393 367 335
268 97
EcoNI CCTnn'n_nnAGG
Cuts at: 2492 3507 2492
Size: 1015 5667
EcoO109I rG'GnC_Cy
Cuts at: 2689 2689
Size: 6682
EcoRI G'AATT_C
Cuts at: 3941 3991 4369 3941
Size: 50 378 6254
Fragments arranged by size:
6254 378 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 3655 2395
Size: 1260 5422
FspI TGC'GCA
Cuts at: 5188 5188
Size: 6682
HaeII r_GCGC'y
Cuts at: 1678 2095 5829 1678
Size: 417 3734 2531
Fragments arranged by size:
3734 2531 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2367 2399 3255 3287 5323
Size: 32 135 32 795 32 856 32 2036
Cuts at: 5323 5355 5397 5429 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2036 856 795 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2667 3814 4026 4272 669
Size: 988 281 729 1147 212 246 3079
Fragments arranged by size:
3079 1147 988 729 281 246 212
HindIII A'AGCT_T
Cuts at: 748 2508 748
Size: 1760 4922
HpaI GTT'AAC
Cuts at: 2667 4272 2667
Size: 1605 5077
KasI G'GCGC_C
Cuts at: 2091 2091
Size: 6682
KpnI G_GTAC'C
Cuts at: 4007 4007
Size: 6682
MfeI C'AATT_G
Cuts at: 2676 4281 2676
Size: 1605 5077
MluI A'CGCG_T
Cuts at: 6558 6558
Size: 6682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5796 5980 1305
Size: 4491 184 2007
Fragments arranged by size:
4491 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4860
Fragments arranged by size:
4860 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3921 4640 4999 5158 518
Size: 648 2755 719 359 159 2042
Fragments arranged by size:
2755 2042 719 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2566 4098 4775 5923 6168 1606
Size: 960 1532 677 1148 245 2120
Fragments arranged by size:
2120 1532 1148 960 677 245
NarI GG'CG_CC
Cuts at: 2092 2092
Size: 6682
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5191
Fragments arranged by size:
5191 939 552
NdeI CA'TA_TG
Cuts at: 387 3918 387
Size: 3531 3151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6682
NotI GC'GGCC_GC
Cuts at: 4129 4129
Size: 6682
NspI r_CATG'y
Cuts at: 1132 1229 4040 5585 1132
Size: 97 2811 1545 2229
Fragments arranged by size:
2811 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6682
PciI A'CATG_T
Cuts at: 5581 5581
Size: 6682
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6682
PmeI GTTT'AAAC
Cuts at: 3986 3986
Size: 6682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4751 4783 6264 6296 4751
Size: 32 1481 32 5137
Fragments arranged by size:
5137 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6682
PsiI TTA'TAA
Cuts at: 2523 3323 4252 2523
Size: 800 929 4953
Fragments arranged by size:
4953 929 800
PspOMI G'GGCC_C
Cuts at: 2689 2689
Size: 6682
PstI C_TGCA'G
Cuts at: 830 4034 830
Size: 3204 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6682
PvuII CAG'CTG
Cuts at: 2566 2566
Size: 6682
SacI G_AGCT'C
Cuts at: 2686 2686
Size: 6682
SalI G'TCGA_C
Cuts at: 1936 3812 4024 1936
Size: 1876 212 4594
Fragments arranged by size:
4594 1876 212
SbfI CC_TGCA'GG
Cuts at: 4034 4034
Size: 6682
ScaI AGT'ACT
Cuts at: 2024 3195 3627 3908 4930 6642 2024
Size: 1171 432 281 1022 1712 2064
Fragments arranged by size:
2064 1712 1171 1022 432 281
SexAI A'CCwGG_T
Cuts at: 3286 3286
Size: 6682
SfcI C'TryA_G
Cuts at: 826 1045 4030 5165 5846 6037 826
Size: 219 2985 1135 681 191 1471
Fragments arranged by size:
2985 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2093 2093
Size: 6682
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6682
SmaI CCC'GGG
Cuts at: 1872 4009 1872
Size: 2137 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2197 3041 3108 4786 5687 5949
Size: 197 926 254 844 67 1678 901 262
Cuts at: 5949 820
Size: 1553
Fragments arranged by size:
1678 1553 926 901 844 262 254 197
67
SnaBI TAC'GTA
Cuts at: 493 4473 493
Size: 3980 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6682
SphI G_CATG'C
Cuts at: 1132 4040 1132
Size: 2908 3774
SspI AAT'ATT
Cuts at: 5 52 4606 5
Size: 47 4554 2081
Fragments arranged by size:
4554 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6682
StyI C'CwwG_G
Cuts at: 513 1065 2004 3550 4121 513
Size: 552 939 1546 571 3074
Fragments arranged by size:
3074 1546 939 571 552
SwaI ATTT'AAAT
Cuts at: 3207 3207
Size: 6682
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4709 4726 4879 5064 5509 325
Size: 1255 3129 17 153 185 445 1498
Fragments arranged by size:
3129 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 3047 3193
Size: 274 80 33 51 1443 45 1025 146
Cuts at: 3193 3265 3625 3906 4928 6640 96
Size: 72 360 281 1022 1712 138
Fragments arranged by size:
1712 1443 1025 1022 360 281 274 146
138 80 72 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6642 6674 6642
Size: 32 6650
Tth111I GACn'n_nGTC
Cuts at: 1316 3647 1316
Size: 2331 4351
XbaI T'CTAG_A
Cuts at: 3064 4018 3064
Size: 954 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6682
XmaI C'CCGG_G
Cuts at: 1870 4007 1870
Size: 2137 4545
XmnI GAAnn'nnTTC
Cuts at: 2366 3442 4811 2366
Size: 1076 1369 4237
Fragments arranged by size:
4237 1369 1076
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 4490 276
Size: 53 83 186 936 1957 999 2468
Fragments arranged by size:
2468 1957 999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SrfI XcmI