Restriction map for
pFN21AB6179
(Circular) (Six-base) MAPSORT of: pFN21AB6179.seq Check: 4385 from: 1 to: 6526
pFN21AB6179 6526 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4336 278
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2406 3869 1937
Size: 469 1463 4594
Fragments arranged by size:
4594 1463 469
Acc65I G'GTAC_C
Cuts at: 3847 3847
Size: 6526
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3077 3150 3635 3713 4589 5973 6241 1632
Size: 1445 73 485 78 876 1384 268 1917
Fragments arranged by size:
1917 1445 1384 876 485 268 78 73
AflII C'TTAA_G
Cuts at: 820 1017 2774 820
Size: 197 1757 4572
Fragments arranged by size:
4572 1757 197
AflIII A'CryG_T
Cuts at: 1203 3288 3744 5425 6402 1203
Size: 2085 456 1681 977 1327
Fragments arranged by size:
2085 1681 1327 977 456
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 5255 5255
Size: 6526
AleI CACnn'nnGTG
Cuts at: 1166 2414 1166
Size: 1248 5278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6526
AlwNI CAG_nnn'CTG
Cuts at: 2768 3664 3790 4269 5841 2768
Size: 896 126 479 1572 3453
Fragments arranged by size:
3453 1572 896 479 126
ApaI G_GGCC'C
Cuts at: 2059 2059
Size: 6526
ApaLI G'TGCA_C
Cuts at: 4583 5739 4583
Size: 1156 5370
ApoI r'AATT_y
Cuts at: 1493 3835 4061 4213 6322 1493
Size: 2342 226 152 2109 1697
Fragments arranged by size:
2342 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2234 2697 3522 3851 1870
Size: 73 253 38 463 825 329 4545
Fragments arranged by size:
4545 825 463 329 253 73 38
BamHI G'GATC_C
Cuts at: 3400 3856 4223 3400
Size: 456 367 5703
Fragments arranged by size:
5703 456 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2062 2086 3847 5302
Size: 325 217 627 224 51 24 1761 1455
Cuts at: 5302 618
Size: 1842
Fragments arranged by size:
1842 1761 1455 627 325 224 217 51
24
BanII G_rGCy'C
Cuts at: 1391 2059 2235 3321 3629 3845 1391
Size: 668 176 1086 308 216 4072
Fragments arranged by size:
4072 1086 668 308 216 176
BbeI G_GCGC'C
Cuts at: 2015 2066 2015
Size: 51 6475
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 2990 3623 928
Size: 971 617 474 633 3831
Fragments arranged by size:
3831 971 633 617 474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4717 4751 1903
Size: 34 2780 34 3678
Fragments arranged by size:
3678 2780 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2439 2632 4418 5628 2439
Size: 193 1786 1210 3337
Fragments arranged by size:
3337 1786 1210 193
BclI T'GATC_A
Cuts at: 1548 2097 1548
Size: 549 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2246 5137
Size: 107 122 71 631 605 337 237 2891
Cuts at: 5137 136
Size: 1525
Fragments arranged by size:
2891 1525 631 605 337 237 122 107
71
BglII A'GATC_T
Cuts at: 6521 6521
Size: 6526
BlpI GC'TnA_GC
Cuts at: 3711 3943 3711
Size: 232 6294
Bme1580I G_kGCm'C
Cuts at: 1790 2059 3181 4587 5743 1790
Size: 269 1122 1406 1156 2573
Fragments arranged by size:
2573 1406 1156 1122 269
BmgBI CAC'GTC
Cuts at: 1318 2110 2193 1318
Size: 792 83 5651
Fragments arranged by size:
5651 792 83
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2351 2440 2728
Size: 416 53 83 399 24 934 89 288
Cuts at: 2728 4257 5215 442
Size: 1529 958 1753
Fragments arranged by size:
1753 1529 958 934 416 399 288 89
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3581 5186 1239
Size: 2342 1605 2579
Fragments arranged by size:
2579 2342 1605
Bpu10I CC'TnA_GC
Cuts at: 2129 2145 2229 2129
Size: 16 84 6426
Fragments arranged by size:
6426 84 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3719 4651 5516 5814 3719
Size: 932 865 298 4431
Fragments arranged by size:
4431 932 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3491 5189 882
Size: 617 1992 1698 2219
Fragments arranged by size:
2219 1992 1698 617
BsaAI yAC'GTr
Cuts at: 493 1204 2306 3747 4317 493
Size: 711 1102 1441 570 2702
Fragments arranged by size:
2702 1441 1102 711 570
BsaBI GATnn'nnATC
Cuts at: 2948 6520 2948
Size: 3572 2954
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2063 4333 4715
Size: 53 83 186 936 479 51 2270 382
Cuts at: 4715 5350 275
Size: 635 1451
Fragments arranged by size:
2270 1451 936 635 479 382 186 83
53 51
BsaWI w'CCGG_w
Cuts at: 4959 5357 5631 5778 6189 4959
Size: 398 274 147 411 5296
Fragments arranged by size:
5296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6301
Fragments arranged by size:
6301 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 1186
Size: 882 5644
BseYI C'CCAG_C
Cuts at: 2566 5729 6225 2566
Size: 3163 496 2867
Fragments arranged by size:
3163 2867 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 3176 1163
Size: 573 546 894 4513
Fragments arranged by size:
4513 894 573 546
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2157 3976 4737 5765 1540
Size: 275 186 156 1819 761 1028 2301
Fragments arranged by size:
2301 1819 1028 761 275 186 156
BsiHKAI G_wGCw'C
Cuts at: 2235 3306 4587 4672 5743 2235
Size: 1071 1281 85 1071 3018
Fragments arranged by size:
3018 1281 1071 1071 85
BsmI GAATG_Cn'
Cuts at: 4037 4130 4037
Size: 93 6433
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2235 3181 3306 3321 3629 3845
Size: 399 269 176 946 125 15 308 216
Cuts at: 3845 4587 4672 5743 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 946 742 399 308 269 216
176 125 85 15
BspEI T'CCGG_A
Cuts at: 6189 6189
Size: 6526
BspHI T'CATG_A
Cuts at: 4415 6145 4415
Size: 1730 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2254 3436 3881 844
Size: 1410 1182 445 3489
Fragments arranged by size:
3489 1410 1182 445
BsrBI CCG'CTC
Cuts at: 1394 1823 4413 1394
Size: 429 2590 3507
Fragments arranged by size:
3507 2590 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2905 5021 5195 65
Size: 1183 1657 2116 174 1396
Fragments arranged by size:
2116 1657 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 5170 5357 1700
Size: 110 211 3149 187 2869
Fragments arranged by size:
3149 2869 211 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6526
BssSI C'ACGA_G
Cuts at: 4586 5598 4586
Size: 1012 5514
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6526
BstBI TT'CG_AA
Cuts at: 5330 5330
Size: 6526
BstEII G'GTnAC_C
Cuts at: 1184 3041 3793 1184
Size: 1857 752 3917
Fragments arranged by size:
3917 1857 752
BstYI r'GATC_y
Cuts at: 1972 3400 3856 4223 4608 4625 6077 6163 6175
Size: 1428 456 367 385 17 1452 86 12
Cuts at: 6175 6521 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1428 456 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5035
Fragments arranged by size:
5035 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5787
Fragments arranged by size:
5787 683 56
BtsI GCAGTG_nn'
Cuts at: 3596 4042 4836 4856 3596
Size: 446 794 20 5266
Fragments arranged by size:
5266 794 446 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6526
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6491
DraI TTT'AAA
Cuts at: 2647 3830 2647
Size: 1183 5343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3278 3500 5533 809
Size: 507 1962 222 2033 1802
Fragments arranged by size:
2033 1962 1802 507 222
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 3217 3973
Size: 54 1425 50 275 18 324 1063 756
Cuts at: 3973 4862 6491 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1063 889 756 324 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2154 3973 1537
Size: 275 342 1819 4090
Fragments arranged by size:
4090 1819 342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 3548 4456 1353
Size: 927 1268 908 3423
Fragments arranged by size:
3423 1268 927 908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5113 5487 5633 387
Size: 1064 138 3524 374 146 1280
Fragments arranged by size:
3524 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2233 2233
Size: 6526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3077 3150 3581 3635 3713 4589 5186
Size: 393 1445 73 431 54 78 876 597
Cuts at: 5186 5973 6241 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1445 876 787 597 431 393 268
78 73 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2144 2144
Size: 6526
EcoO109I rG'GnC_Cy
Cuts at: 2056 2590 2780 2056
Size: 534 190 5802
Fragments arranged by size:
5802 534 190
EcoRI G'AATT_C
Cuts at: 3835 4213 3835
Size: 378 6148
FspI TGC'GCA
Cuts at: 5032 5032
Size: 6526
HaeII r_GCGC'y
Cuts at: 1678 2015 2066 2076 3718 3784 5673 1678
Size: 337 51 10 1642 66 1889 2531
Fragments arranged by size:
2531 1889 1642 337 66 51 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3088 3120 5167 5199 5241
Size: 32 135 32 1516 32 2047 32 42
Cuts at: 5241 5273 1373
Size: 32 2626
Fragments arranged by size:
2626 2047 1516 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3463 3870 4116 669
Size: 988 281 1525 407 246 3079
Fragments arranged by size:
3079 1525 988 407 281 246
HindIII A'AGCT_T
Cuts at: 748 2531 748
Size: 1783 4743
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KasI G'GCGC_C
Cuts at: 2011 2062 2011
Size: 51 6475
KpnI G_GTAC'C
Cuts at: 3851 3851
Size: 6526
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 6526
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2456 2528 5640 5824 1305
Size: 1151 72 3112 184 2007
Fragments arranged by size:
3112 2007 1151 184 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4704
Fragments arranged by size:
4704 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 2525 2690 4484 4843 5002 518
Size: 648 1248 111 165 1794 359 159 2042
Fragments arranged by size:
2042 1794 1248 648 359 165 159 111
MspA1I CmG'CkG
Cuts at: 1606 2095 3942 4619 5767 6012 1606
Size: 489 1847 677 1148 245 2120
Fragments arranged by size:
2120 1847 1148 677 489 245
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 6526
NarI GG'CG_CC
Cuts at: 2012 2063 2012
Size: 51 6475
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5035
Fragments arranged by size:
5035 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 6526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 3884 5429 1132
Size: 97 2655 1545 2229
Fragments arranged by size:
2655 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6526
PciI A'CATG_T
Cuts at: 5425 5425
Size: 6526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2819 3790 3814 1108
Size: 1711 971 24 3820
Fragments arranged by size:
3820 1711 971 24
PfoI T'CCnGG_A
Cuts at: 3813 3813
Size: 6526
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2590 2590
Size: 6526
PshAI GACnn'nnGTC
Cuts at: 1613 2699 3458 1613
Size: 1086 759 4681
Fragments arranged by size:
4681 1086 759
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PspOMI G'GGCC_C
Cuts at: 2055 2055
Size: 6526
PspXI vC'TCGA_Gb
Cuts at: 2234 2234
Size: 6526
PstI C_TGCA'G
Cuts at: 830 3315 3606 3878 830
Size: 2485 291 272 3478
Fragments arranged by size:
3478 2485 291 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
SacI G_AGCT'C
Cuts at: 2235 2235
Size: 6526
SalI G'TCGA_C
Cuts at: 1936 3868 1936
Size: 1932 4594
SapI GCTCTTCn'nnn_
Cuts at: 3548 3548
Size: 6526
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2629 2937 3311 3602 3874 5009 5690
Size: 219 1584 308 374 291 272 1135 681
Cuts at: 5690 5881 826
Size: 191 1471
Fragments arranged by size:
1584 1471 1135 681 374 308 291 272
219 191
SfoI GGC'GCC
Cuts at: 2013 2064 2013
Size: 51 6475
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 3524 3853 1872
Size: 1652 329 4545
Fragments arranged by size:
4545 1652 329
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 2774 3734 4630 5531 5793
Size: 197 926 291 540 960 896 901 262
Cuts at: 5793 820
Size: 1553
Fragments arranged by size:
1553 960 926 901 896 540 291 262
197
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 3884 1132
Size: 2752 3774
SspI AAT'ATT
Cuts at: 5 52 2728 4450 5
Size: 47 2676 1722 2081
Fragments arranged by size:
2676 2081 1722 47
StuI AGG'CCT
Cuts at: 1517 3477 3578 1517
Size: 1960 101 4465
Fragments arranged by size:
4465 1960 101
StyI C'CwwG_G
Cuts at: 513 1065 2004 3396 3965 513
Size: 552 939 1392 569 3074
Fragments arranged by size:
3074 1392 939 569 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2426 2459 3029 4553 4570 4723 4908
Size: 1255 846 33 570 1524 17 153 185
Cuts at: 4908 5353 325
Size: 445 1498
Fragments arranged by size:
1524 1498 1255 846 570 445 185 153
33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2650 4772 6484
Size: 274 80 33 51 1443 673 2122 1712
Cuts at: 6484 96
Size: 138
Fragments arranged by size:
2122 1712 1443 673 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3385 3417 6486 6518 3385
Size: 32 3069 32 3393
Fragments arranged by size:
3393 3069 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6526
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2996 2996
Size: 6526
XhoI C'TCGA_G
Cuts at: 1943 2234 1943
Size: 291 6235
XmaI C'CCGG_G
Cuts at: 1870 3522 3851 1870
Size: 1652 329 4545
Fragments arranged by size:
4545 1652 329
XmnI GAAnn'nnTTC
Cuts at: 4655 4655
Size: 6526
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4334 276
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI