Restriction map for
pFN21AB6172
(Circular) (Six-base) MAPSORT of: pFN21AB6172.seq Check: 8103 from: 1 to: 6517
pFN21AB6172 6517 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3470 4327 278
Size: 53 83 186 936 1934 857 2468
Fragments arranged by size:
2468 1934 936 857 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2105 3406 3860 1937
Size: 168 1301 454 4594
Fragments arranged by size:
4594 1301 454 168
Acc65I G'GTAC_C
Cuts at: 3838 3838
Size: 6517
AclI AA'CG_TT
Cuts at: 1563 4644 5017 1563
Size: 3081 373 3063
Fragments arranged by size:
3081 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 3576 4580 5964 6232 1632
Size: 702 1242 1004 1384 268 1917
Fragments arranged by size:
1917 1384 1242 1004 702 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6320
AflIII A'CryG_T
Cuts at: 1203 2058 3671 5416 6393 1203
Size: 855 1613 1745 977 1327
Fragments arranged by size:
1745 1613 1327 977 855
AgeI A'CCGG_T
Cuts at: 5348 5348
Size: 6517
AhdI GACnn_n'nnGTC
Cuts at: 5246 5246
Size: 6517
AleI CACnn'nnGTG
Cuts at: 1166 2180 1166
Size: 1014 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2784 1184
Size: 1600 4917
AlwNI CAG_nnn'CTG
Cuts at: 3527 4260 5832 3527
Size: 733 1572 4212
Fragments arranged by size:
4212 1572 733
ApaLI G'TGCA_C
Cuts at: 4574 5730 4574
Size: 1156 5361
ApoI r'AATT_y
Cuts at: 1493 2038 2302 2359 3826 4052 4204 6313 1493
Size: 545 264 57 1467 226 152 2109 1697
Fragments arranged by size:
2109 1697 1467 545 264 226 152 57
AseI AT'TA_AT
Cuts at: 160 1032 3150 3341 3426 5071 160
Size: 872 2118 191 85 1645 1606
Fragments arranged by size:
2118 1645 1606 872 191 85
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
AvaI C'yCGr_G
Cuts at: 1870 1943 3842 1870
Size: 73 1899 4545
Fragments arranged by size:
4545 1899 73
BamHI G'GATC_C
Cuts at: 3847 4214 3847
Size: 367 6150
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3838 5293 618
Size: 325 217 627 2051 1455 1842
Fragments arranged by size:
2051 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3836 1391
Size: 2445 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3114 3464 928
Size: 971 1215 350 3981
Fragments arranged by size:
3981 1215 971 350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4708 4742 1903
Size: 34 2771 34 3678
Fragments arranged by size:
3678 2771 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2283 4409 5619 2283
Size: 2126 1210 3181
Fragments arranged by size:
3181 2126 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6517
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5128 136
Size: 107 122 71 631 605 3456 1525
Fragments arranged by size:
3456 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2566 3625 6512 2566
Size: 1059 2887 2571
Fragments arranged by size:
2887 2571 1059
BlpI GC'TnA_GC
Cuts at: 3934 3934
Size: 6517
Bme1580I G_kGCm'C
Cuts at: 1790 4578 5734 1790
Size: 2788 1156 2573
Fragments arranged by size:
2788 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4248 5206 442
Size: 416 53 83 399 24 2831 958 1753
Fragments arranged by size:
2831 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6517
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2172 2172
Size: 6517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2431 2622 2937 3123 3243 5177 1239
Size: 1192 191 315 186 120 1934 2579
Fragments arranged by size:
2579 1934 1192 315 191 186 120
Bpu10I CC'TnA_GC
Cuts at: 2823 2823
Size: 6517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4642 5507 5805 4642
Size: 865 298 5354
Fragments arranged by size:
5354 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5180 882
Size: 617 3681 2219
Fragments arranged by size:
3681 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2109 4308 493
Size: 711 905 2199 2702
Fragments arranged by size:
2702 2199 905 711
BsaBI GATnn'nnATC
Cuts at: 6511 6511
Size: 6517
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3467 4324 4706 5341
Size: 53 83 186 936 1934 857 382 635
Cuts at: 5341 275
Size: 1451
Fragments arranged by size:
1934 1451 936 857 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3345 4950 5348 5622 5769 6180 3345
Size: 1605 398 274 147 411 3682
Fragments arranged by size:
3682 1605 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2595 2625 1178
Size: 30 165 30 1192 30 5070
Fragments arranged by size:
5070 1192 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2253 3308 1186
Size: 1067 1055 4395
Fragments arranged by size:
4395 1067 1055
BseYI C'CCAG_C
Cuts at: 2126 5720 6216 2126
Size: 3594 496 2427
Fragments arranged by size:
3594 2427 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5944
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3967 4728 5756 1540
Size: 275 186 1966 761 1028 2301
Fragments arranged by size:
2301 1966 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3751 4578 4663 5734 3751
Size: 827 85 1071 4534
Fragments arranged by size:
4534 1071 827 85
BsmI GAATG_Cn'
Cuts at: 4028 4121 4028
Size: 93 6424
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3751 3836 4578 4663 5734 1391
Size: 399 1961 85 742 85 1071 2174
Fragments arranged by size:
2174 1961 1071 742 399 85 85
BspEI T'CCGG_A
Cuts at: 3345 6180 3345
Size: 2835 3682
BspHI T'CATG_A
Cuts at: 3129 4406 6136 3129
Size: 1277 1730 3510
Fragments arranged by size:
3510 1730 1277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3872 844
Size: 3028 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4404 1394
Size: 429 2581 3507
Fragments arranged by size:
3507 2581 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2505 5012 5186 65
Size: 1183 1257 2507 174 1396
Fragments arranged by size:
2507 1396 1257 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5161 5348 1700
Size: 110 3351 187 2869
Fragments arranged by size:
3351 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6517
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6517
BssSI C'ACGA_G
Cuts at: 2157 4577 5589 2157
Size: 2420 1012 3085
Fragments arranged by size:
3085 2420 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6517
BstBI TT'CG_AA
Cuts at: 5321 5321
Size: 6517
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6517
BstYI r'GATC_y
Cuts at: 1972 2566 3625 3847 4214 4599 4616 6068 6154
Size: 594 1059 222 367 385 17 1452 86
Cuts at: 6154 6166 6512 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1059 594 385 367 346 222
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2407 2927 3159 513
Size: 552 1342 520 232 3871
Fragments arranged by size:
3871 1342 552 520 232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5778
Fragments arranged by size:
5778 683 56
BtsI GCAGTG_nn'
Cuts at: 4033 4827 4847 4033
Size: 794 20 5703
Fragments arranged by size:
5703 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6482
DraI TTT'AAA
Cuts at: 2782 3821 2782
Size: 1039 5478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2554 3466 5524 809
Size: 507 1238 912 2058 1802
Fragments arranged by size:
2058 1802 1238 912 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2540 3964 4853
Size: 54 1425 50 275 18 710 1424 889
Cuts at: 4853 6482 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1424 889 710 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3964 1537
Size: 275 2152 4090
Fragments arranged by size:
4090 2152 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3285 4447 1353
Size: 1932 1162 3423
Fragments arranged by size:
3423 1932 1162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5104 5478 5624 387
Size: 1064 138 3515 374 146 1280
Fragments arranged by size:
3515 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2431 2622 2937 3123 3243 3576
Size: 393 702 97 191 315 186 120 333
Cuts at: 3576 4580 5177 5964 6232 1239
Size: 1004 597 787 268 1524
Fragments arranged by size:
1524 1004 787 702 597 393 333 315
268 191 186 120 97
EcoO109I rG'GnC_Cy
Cuts at: 2597 2597
Size: 6517
EcoRI G'AATT_C
Cuts at: 3826 4204 3826
Size: 378 6139
FspI TGC'GCA
Cuts at: 5023 5023
Size: 6517
HaeII r_GCGC'y
Cuts at: 1678 2055 5664 1678
Size: 377 3609 2531
Fragments arranged by size:
3609 2531 377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3124 3156 5158 5190 5232
Size: 32 135 32 1552 32 2002 32 42
Cuts at: 5232 5264 1373
Size: 32 2626
Fragments arranged by size:
2626 2002 1552 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2311 3861 4107 669
Size: 988 281 373 1550 246 3079
Fragments arranged by size:
3079 1550 988 373 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6517
HpaI GTT'AAC
Cuts at: 2311 4107 2311
Size: 1796 4721
KpnI G_GTAC'C
Cuts at: 3842 3842
Size: 6517
MfeI C'AATT_G
Cuts at: 2644 3644 4116 2644
Size: 1000 472 5045
Fragments arranged by size:
5045 1000 472
MluI A'CGCG_T
Cuts at: 6393 6393
Size: 6517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5631 5815 1305
Size: 4326 184 2007
Fragments arranged by size:
4326 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2542 10
Size: 54 1425 343 710 3985
Fragments arranged by size:
3985 1425 710 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 4475 4834 4993 518
Size: 648 1014 2295 359 159 2042
Fragments arranged by size:
2295 2042 1014 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3037 3933 4610 5758 6003 1606
Size: 1431 896 677 1148 245 2120
Fragments arranged by size:
2120 1431 1148 896 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2927 513
Size: 552 1862 4103
Fragments arranged by size:
4103 1862 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6517
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6517
NotI GC'GGCC_GC
Cuts at: 3964 3964
Size: 6517
NspI r_CATG'y
Cuts at: 1132 1229 2761 2771 2942 3675 3875 5420 1132
Size: 97 1532 10 171 733 200 1545 2229
Fragments arranged by size:
2229 1545 1532 733 200 171 97 10
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6517
PciI A'CATG_T
Cuts at: 3671 5416 3671
Size: 1745 4772
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6517
PfoI T'CCnGG_A
Cuts at: 3396 3396
Size: 6517
PmeI GTTT'AAAC
Cuts at: 3821 3821
Size: 6517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4586 4618 6099 6131 4586
Size: 32 1481 32 4972
Fragments arranged by size:
4972 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2597 2597
Size: 6517
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6517
PsiI TTA'TAA
Cuts at: 4087 4087
Size: 6517
PstI C_TGCA'G
Cuts at: 830 2285 3869 830
Size: 1455 1584 3478
Fragments arranged by size:
3478 1584 1455
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6517
PvuII CAG'CTG
Cuts at: 3037 3037
Size: 6517
SalI G'TCGA_C
Cuts at: 1936 3859 1936
Size: 1923 4594
SbfI CC_TGCA'GG
Cuts at: 3869 3869
Size: 6517
ScaI AGT'ACT
Cuts at: 2966 4765 6477 2966
Size: 1799 1712 3006
Fragments arranged by size:
3006 1799 1712
SfcI C'TryA_G
Cuts at: 826 1045 2281 3865 5000 5681 5872 826
Size: 219 1236 1584 1135 681 191 1471
Fragments arranged by size:
1584 1471 1236 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6517
SmaI CCC'GGG
Cuts at: 1872 3844 1872
Size: 1972 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4621 5522 5784 820
Size: 197 926 2678 901 262 1553
Fragments arranged by size:
2678 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2109 4308 493
Size: 1616 2199 2702
Fragments arranged by size:
2702 2199 1616
SpeI A'CTAG_T
Cuts at: 152 3238 152
Size: 3086 3431
SphI G_CATG'C
Cuts at: 1132 2761 3875 1132
Size: 1629 1114 3774
Fragments arranged by size:
3774 1629 1114
SspI AAT'ATT
Cuts at: 5 52 3517 4441 5
Size: 47 3465 924 2081
Fragments arranged by size:
3465 2081 924 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6517
StyI C'CwwG_G
Cuts at: 513 1065 2927 2954 3956 513
Size: 552 1862 27 1002 3074
Fragments arranged by size:
3074 1862 1002 552 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4544 4561 4714 4899 5344 325
Size: 1255 2964 17 153 185 445 1498
Fragments arranged by size:
2964 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2964 3782 4763
Size: 274 80 33 51 1443 987 818 981
Cuts at: 4763 6475 96
Size: 1712 138
Fragments arranged by size:
1712 1443 987 981 818 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2595 2627 6477 6509 2595
Size: 32 3850 32 2603
Fragments arranged by size:
3850 2603 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2192 1316
Size: 876 5641
XbaI T'CTAG_A
Cuts at: 3853 3853
Size: 6517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3098 3098
Size: 6517
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6517
XmaI C'CCGG_G
Cuts at: 1870 3842 1870
Size: 1972 4545
XmnI GAAnn'nnTTC
Cuts at: 4646 4646
Size: 6517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3468 4325 276
Size: 53 83 186 936 1934 857 2468
Fragments arranged by size:
2468 1934 936 857 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI