Restriction map for
pFN21AB6171
(Circular) (Six-base) MAPSORT of: pFN21AB6171.seq Check: 6037 from: 1 to: 6652
pFN21AB6171 6652 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3843 3843
Size: 6652
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4462 278
Size: 53 83 186 936 2926 2468
Fragments arranged by size:
2926 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3995 1937
Size: 2058 4594
Acc65I G'GTAC_C
Cuts at: 3973 3973
Size: 6652
AclI AA'CG_TT
Cuts at: 1563 4779 5152 1563
Size: 3216 373 3063
Fragments arranged by size:
3216 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2644 4715 6099 6367 1632
Size: 767 245 2071 1384 268 1917
Fragments arranged by size:
2071 1917 1384 767 268 245
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6455
AflIII A'CryG_T
Cuts at: 1203 5551 6528 1203
Size: 4348 977 1327
Fragments arranged by size:
4348 1327 977
AgeI A'CCGG_T
Cuts at: 5483 5483
Size: 6652
AhdI GACnn_n'nnGTC
Cuts at: 5381 5381
Size: 6652
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6652
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 6620
AlwNI CAG_nnn'CTG
Cuts at: 3940 4395 5967 3940
Size: 455 1572 4625
Fragments arranged by size:
4625 1572 455
ApaI G_GGCC'C
Cuts at: 2063 2063
Size: 6652
ApaLI G'TGCA_C
Cuts at: 3118 4709 5865 3118
Size: 1591 1156 3905
Fragments arranged by size:
3905 1591 1156
ApoI r'AATT_y
Cuts at: 1493 2815 3961 4187 4339 6448 1493
Size: 1322 1146 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 1146 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5206 160
Size: 872 4174 1606
Fragments arranged by size:
4174 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6652
AvaI C'yCGr_G
Cuts at: 1870 1943 2665 3977 1870
Size: 73 722 1312 4545
Fragments arranged by size:
4545 1312 722 73
AvrII C'CTAG_G
Cuts at: 3847 3847
Size: 6652
BamHI G'GATC_C
Cuts at: 3982 4349 3982
Size: 367 6285
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2068 2725 3973 5428
Size: 325 217 627 221 60 657 1248 1455
Cuts at: 5428 618
Size: 1842
Fragments arranged by size:
1842 1455 1248 657 627 325 221 217
60
BanII G_rGCy'C
Cuts at: 1391 2063 3108 3563 3951 3971 1391
Size: 672 1045 455 388 20 4072
Fragments arranged by size:
4072 1045 672 455 388 20
BbeI G_GCGC'C
Cuts at: 2072 2072
Size: 6652
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2221 3652 928
Size: 971 322 1431 3928
Fragments arranged by size:
3928 1431 971 322
BbvCI CC'TCA_GC
Cuts at: 2763 2763
Size: 6652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4843 4877 1903
Size: 34 2906 34 3678
Fragments arranged by size:
3678 2906 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3024 4544 5754 3024
Size: 1520 1210 3922
Fragments arranged by size:
3922 1520 1210
BclI T'GATC_A
Cuts at: 1548 2494 2991 1548
Size: 946 497 5209
Fragments arranged by size:
5209 946 497
BfrBI ATG'CAT
Cuts at: 2842 2842
Size: 6652
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3609 3771 5263
Size: 107 122 71 631 605 1937 162 1492
Cuts at: 5263 136
Size: 1525
Fragments arranged by size:
1937 1525 1492 631 605 162 122 107
71
BglII A'GATC_T
Cuts at: 3180 6647 3180
Size: 3467 3185
BlpI GC'TnA_GC
Cuts at: 4069 4069
Size: 6652
Bme1580I G_kGCm'C
Cuts at: 1790 2063 3122 3235 3864 4713 5869 1790
Size: 273 1059 113 629 849 1156 2573
Fragments arranged by size:
2573 1156 1059 849 629 273 113
BmgBI CAC'GTC
Cuts at: 1318 3139 1318
Size: 1821 4831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3229 4383 5341
Size: 416 53 83 399 24 1812 1154 958
Cuts at: 5341 442
Size: 1753
Fragments arranged by size:
1812 1753 1154 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2753 3000 3161 3825 5312 1239
Size: 1514 247 161 664 1487 2579
Fragments arranged by size:
2579 1514 1487 664 247 161
Bpu10I CC'TnA_GC
Cuts at: 2763 2985 2763
Size: 222 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4777 5642 5940 4777
Size: 865 298 5489
Fragments arranged by size:
5489 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 2873 5315 882
Size: 617 858 516 2442 2219
Fragments arranged by size:
2442 2219 858 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 4443 493
Size: 711 3239 2702
Fragments arranged by size:
3239 2702 711
BsaBI GATnn'nnATC
Cuts at: 3012 6646 3012
Size: 3634 3018
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 4459 4841 5476
Size: 53 83 186 936 536 2390 382 635
Cuts at: 5476 275
Size: 1451
Fragments arranged by size:
2390 1451 936 635 536 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2271 2472 5085 5483 5757 5904 6315 2271
Size: 201 2613 398 274 147 411 2608
Fragments arranged by size:
2613 2608 411 398 274 201 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2973 3003 3425 3455 1178
Size: 30 165 30 1570 30 422 30 4375
Fragments arranged by size:
4375 1570 422 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2213 2780 1186
Size: 970 57 567 5058
Fragments arranged by size:
5058 970 567 57
BseYI C'CCAG_C
Cuts at: 3343 3562 3607 5855 6351 3343
Size: 219 45 2248 496 3644
Fragments arranged by size:
3644 2248 496 219 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6079
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2152 4102 4863 5891 1540
Size: 275 186 151 1950 761 1028 2301
Fragments arranged by size:
2301 1950 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2627 3122 3951 4713 4798 5869 2627
Size: 495 829 762 85 1071 3410
Fragments arranged by size:
3410 1071 829 762 495 85
BsmI GAATG_Cn'
Cuts at: 3078 4163 4256 3078
Size: 1085 93 5474
Fragments arranged by size:
5474 1085 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2446 2446
Size: 6652
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2627 3108 3122 3235 3563 3864
Size: 399 273 564 481 14 113 328 301
Cuts at: 3864 3951 3971 4713 4798 5869 1391
Size: 87 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 564 481 399 328 301
273 113 87 85 20 14
BspEI T'CCGG_A
Cuts at: 2271 6315 2271
Size: 4044 2608
BspHI T'CATG_A
Cuts at: 4541 6271 4541
Size: 1730 4922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 3843 4007 844
Size: 1945 1054 164 3489
Fragments arranged by size:
3489 1945 1054 164
BsrBI CCG'CTC
Cuts at: 1394 1823 4539 1394
Size: 429 2716 3507
Fragments arranged by size:
3507 2716 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3060 3684 5147 5321 65
Size: 1183 1812 624 1463 174 1396
Fragments arranged by size:
1812 1463 1396 1183 624 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3471 5296 5483 1700
Size: 110 1661 1825 187 2869
Fragments arranged by size:
2869 1825 1661 187 110
BsrGI T'GTAC_A
Cuts at: 96 2934 96
Size: 2838 3814
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6652
BssSI C'ACGA_G
Cuts at: 2966 4712 5724 2966
Size: 1746 1012 3894
Fragments arranged by size:
3894 1746 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6652
BstBI TT'CG_AA
Cuts at: 5456 5456
Size: 6652
BstEII G'GTnAC_C
Cuts at: 1184 2776 3058 1184
Size: 1592 282 4778
Fragments arranged by size:
4778 1592 282
BstXI CCAn_nnnn'nTGG
Cuts at: 2605 3406 2605
Size: 801 5851
BstYI r'GATC_y
Cuts at: 1972 3180 3545 3982 4349 4734 4751 6203 6289
Size: 1208 365 437 367 385 17 1452 86
Cuts at: 6289 6301 6647 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1208 437 385 367 365 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2064 2064
Size: 6652
BtgI C'CryG_G
Cuts at: 513 1065 2004 2119 2146 2193 2898 513
Size: 552 939 115 27 47 705 4267
Fragments arranged by size:
4267 939 705 552 115 47 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5913
Fragments arranged by size:
5913 683 56
BtsI GCAGTG_nn'
Cuts at: 2219 4168 4962 4982 2219
Size: 1949 794 20 3889
Fragments arranged by size:
3889 1949 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6652
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3195 3230 3580 3615 529
Size: 35 2631 35 350 35 3566
Fragments arranged by size:
3566 2631 350 35 35 35
DraI TTT'AAA
Cuts at: 3382 3956 3382
Size: 574 6078
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5659 809
Size: 507 4343 1802
Fragments arranged by size:
4343 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2149 3416 3507
Size: 54 1425 50 275 18 319 1267 91
Cuts at: 3507 3601 4099 4988 6617 8
Size: 94 498 889 1629 43
Fragments arranged by size:
1629 1425 1267 889 498 319 275 94
91 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2149 4099 1537
Size: 275 337 1950 4090
Fragments arranged by size:
4090 1950 337 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2940 3333 4582 1353
Size: 1587 393 1249 3423
Fragments arranged by size:
3423 1587 1249 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2438 5239 5613 5759 387
Size: 1064 138 849 2801 374 146 1280
Fragments arranged by size:
2801 1280 1064 849 374 146 138
EcoICRI GAG'CTC
Cuts at: 3949 3949
Size: 6652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2644 2753 3000 3161 3825 4715
Size: 393 767 245 109 247 161 664 890
Cuts at: 4715 5312 6099 6367 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 890 787 767 664 597 393 268
247 245 161 109
EcoO109I rG'GnC_Cy
Cuts at: 2060 2374 3906 2060
Size: 314 1532 4806
Fragments arranged by size:
4806 1532 314
EcoRI G'AATT_C
Cuts at: 3961 4339 3961
Size: 378 6274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3520 3520
Size: 6652
FspI TGC'GCA
Cuts at: 5158 5158
Size: 6652
HaeII r_GCGC'y
Cuts at: 1678 2072 2416 5799 1678
Size: 394 344 3383 2531
Fragments arranged by size:
3383 2531 394 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2007 2039 2047 2079 2411
Size: 32 135 32 435 32 8 32 332
Cuts at: 2411 2443 2479 2511 2783 2815 2983 3015 3315
Size: 32 36 32 272 32 168 32 300
Cuts at: 3315 3347 3662 3694 5293 5325 5367 5399 1373
Size: 32 315 32 1599 32 42 32 2626
Fragments arranged by size:
2626 1599 435 332 315 300 272 168
135 42 36 32 32 32 32 32
32 32 32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3996 4242 669
Size: 988 281 2058 246 3079
Fragments arranged by size:
3079 2058 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6652
HpaI GTT'AAC
Cuts at: 4242 4242
Size: 6652
KasI G'GCGC_C
Cuts at: 2068 2068
Size: 6652
KpnI G_GTAC'C
Cuts at: 3977 3977
Size: 6652
MfeI C'AATT_G
Cuts at: 4251 4251
Size: 6652
MluI A'CGCG_T
Cuts at: 6528 6528
Size: 6652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2641 2664 3615 3939 5766 5950 1305
Size: 1336 23 951 324 1827 184 2007
Fragments arranged by size:
2007 1827 1336 951 324 184 23
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3418 3509 3603 10
Size: 54 1425 343 1586 91 94 3059
Fragments arranged by size:
3059 1586 1425 343 94 91 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2897 4610 4969 5128 518
Size: 648 972 759 1713 359 159 2042
Fragments arranged by size:
2042 1713 972 759 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2148 2482 3607 4068 4745 5893 6138 1606
Size: 542 334 1125 461 677 1148 245 2120
Fragments arranged by size:
2120 1148 1125 677 542 461 334 245
NaeI GCC'GGC
Cuts at: 3473 3473
Size: 6652
NarI GG'CG_CC
Cuts at: 2069 2069
Size: 6652
NcoI C'CATG_G
Cuts at: 513 1065 2004 2119 2898 513
Size: 552 939 115 779 4267
Fragments arranged by size:
4267 939 779 552 115
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6652
NgoMIV G'CCGG_C
Cuts at: 3471 3471
Size: 6652
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6652
NotI GC'GGCC_GC
Cuts at: 4099 4099
Size: 6652
NsiI A_TGCA'T
Cuts at: 2844 2844
Size: 6652
NspI r_CATG'y
Cuts at: 1132 1229 4010 5555 1132
Size: 97 2781 1545 2229
Fragments arranged by size:
2781 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6652
PciI A'CATG_T
Cuts at: 5551 5551
Size: 6652
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3717 3940 1108
Size: 2609 223 3820
Fragments arranged by size:
3820 2609 223
PfoI T'CCnGG_A
Cuts at: 2405 2429 3333 2405
Size: 24 904 5724
Fragments arranged by size:
5724 904 24
PmeI GTTT'AAAC
Cuts at: 3956 3956
Size: 6652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2426 2458 4721 4753 6234 6266 2426
Size: 32 2263 32 1481 32 2812
Fragments arranged by size:
2812 2263 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6652
PsiI TTA'TAA
Cuts at: 4222 4222
Size: 6652
PspOMI G'GGCC_C
Cuts at: 2059 2059
Size: 6652
PstI C_TGCA'G
Cuts at: 830 2293 3262 3377 4004 830
Size: 1463 969 115 627 3478
Fragments arranged by size:
3478 1463 969 627 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6652
PvuII CAG'CTG
Cuts at: 3607 3607
Size: 6652
SacI G_AGCT'C
Cuts at: 3951 3951
Size: 6652
SacII CC_GC'GG
Cuts at: 2149 2149
Size: 6652
SalI G'TCGA_C
Cuts at: 1936 3994 1936
Size: 2058 4594
SapI GCTCTTCn'nnn_
Cuts at: 3333 3333
Size: 6652
SbfI CC_TGCA'GG
Cuts at: 4004 4004
Size: 6652
ScaI AGT'ACT
Cuts at: 4900 6612 4900
Size: 1712 4940
SfcI C'TryA_G
Cuts at: 826 1045 2289 3066 3109 3258 3373 4000 5135
Size: 219 1244 777 43 149 115 627 1135
Cuts at: 5135 5816 6007 826
Size: 681 191 1471
Fragments arranged by size:
1471 1244 1135 777 681 627 219 191
149 115 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 3609 3609
Size: 6652
SfoI GGC'GCC
Cuts at: 2070 2070
Size: 6652
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6652
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6652
SmaI CCC'GGG
Cuts at: 1872 3979 1872
Size: 2107 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4756 5657 5919 820
Size: 197 926 2813 901 262 1553
Fragments arranged by size:
2813 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4443 493
Size: 3950 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6652
SphI G_CATG'C
Cuts at: 1132 4010 1132
Size: 2878 3774
SspI AAT'ATT
Cuts at: 5 52 4576 5
Size: 47 4524 2081
Fragments arranged by size:
4524 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6652
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 2898 3466 3711 3847 4091
Size: 552 939 115 779 568 245 136 244
Cuts at: 4091 513
Size: 3074
Fragments arranged by size:
3074 939 779 568 552 245 244 136
115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2132 3216 4679 4696 4849 5034 5479
Size: 1255 552 1084 1463 17 153 185 445
Cuts at: 5479 325
Size: 1498
Fragments arranged by size:
1498 1463 1255 1084 552 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2934 3237
Size: 274 80 33 51 1443 345 612 303
Cuts at: 3237 4898 6610 96
Size: 1661 1712 138
Fragments arranged by size:
1712 1661 1443 612 345 303 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 2973 3005 3843 3875 6612 6644 2579
Size: 32 362 32 838 32 2737 32 2587
Fragments arranged by size:
2737 2587 838 362 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3085 1316
Size: 1769 4883
XbaI T'CTAG_A
Cuts at: 3988 3988
Size: 6652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 2840 2605
Size: 235 6417
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6652
XmaI C'CCGG_G
Cuts at: 1870 3977 1870
Size: 2107 4545
XmnI GAAnn'nnTTC
Cuts at: 4781 4781
Size: 6652
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4460 276
Size: 53 83 186 936 2926 2468
Fragments arranged by size:
2926 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BplI BsiWI BstZ17I DraIII EcoNI
EcoRV FseI FspAI NruI PacI PmlI PpuMI PspXI
PsrI RsrII SanDI SexAI SrfI SwaI