Restriction map for
pFN21AB6169
(Circular) (Six-base) MAPSORT of: pFN21AB6169.seq Check: 324 from: 1 to: 6655
pFN21AB6169 6655 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4465 278
Size: 53 83 186 936 2929 2468
Fragments arranged by size:
2929 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2439 3998 1937
Size: 502 1559 4594
Fragments arranged by size:
4594 1559 502
Acc65I G'GTAC_C
Cuts at: 3057 3976 3057
Size: 919 5736
AclI AA'CG_TT
Cuts at: 1563 4782 5155 1563
Size: 3219 373 3063
Fragments arranged by size:
3219 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3180 3652 4718 6102 6370 1632
Size: 1548 472 1066 1384 268 1917
Fragments arranged by size:
1917 1548 1384 1066 472 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6458
AflIII A'CryG_T
Cuts at: 1203 5554 6531 1203
Size: 4351 977 1327
Fragments arranged by size:
4351 1327 977
AgeI A'CCGG_T
Cuts at: 5486 5486
Size: 6655
AhdI GACnn_n'nnGTC
Cuts at: 3670 3700 5384 3670
Size: 30 1684 4941
Fragments arranged by size:
4941 1684 30
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6655
AlwNI CAG_nnn'CTG
Cuts at: 2261 4398 5970 2261
Size: 2137 1572 2946
Fragments arranged by size:
2946 2137 1572
ApaI G_GGCC'C
Cuts at: 2772 3044 3353 3478 2772
Size: 272 309 125 5949
Fragments arranged by size:
5949 309 272 125
ApaLI G'TGCA_C
Cuts at: 2063 2251 4712 5868 2063
Size: 188 2461 1156 2850
Fragments arranged by size:
2850 2461 1156 188
ApoI r'AATT_y
Cuts at: 1493 3964 4190 4342 6451 1493
Size: 2471 226 152 2109 1697
Fragments arranged by size:
2471 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5209 160
Size: 872 4177 1606
Fragments arranged by size:
4177 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6655
AvaI C'yCGr_G
Cuts at: 1870 1943 2918 3345 3471 3625 3645 3675 3720
Size: 73 975 427 126 154 20 30 45
Cuts at: 3720 3729 3900 3980 1870
Size: 9 171 80 4545
Fragments arranged by size:
4545 975 427 171 154 126 80 73
45 30 20 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3700 3733 3700
Size: 33 6622
BamHI G'GATC_C
Cuts at: 3985 4352 3985
Size: 367 6288
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2847 2886 3057 3976 5431
Size: 325 217 627 1060 39 171 919 1455
Cuts at: 5431 618
Size: 1842
Fragments arranged by size:
1842 1455 1060 919 627 325 217 171
39
BanII G_rGCy'C
Cuts at: 1391 2172 2189 2712 2748 2772 3044 3353 3429
Size: 781 17 523 36 24 272 309 76
Cuts at: 3429 3478 3974 1391
Size: 49 496 4072
Fragments arranged by size:
4072 781 523 496 309 272 76 49
36 24 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2215 2784 928
Size: 971 316 569 4799
Fragments arranged by size:
4799 971 569 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4846 4880 1903
Size: 34 50 34 2825 34 3678
Fragments arranged by size:
3678 2825 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3062 4547 5757 3062
Size: 1485 1210 3960
Fragments arranged by size:
3960 1485 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6655
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2141 2150 2462
Size: 107 122 71 631 605 469 9 312
Cuts at: 2462 5266 136
Size: 2804 1525
Fragments arranged by size:
2804 1525 631 605 469 312 122 107
71 9
BglII A'GATC_T
Cuts at: 6650 6650
Size: 6655
BlpI GC'TnA_GC
Cuts at: 2240 2742 3097 4072 2240
Size: 502 355 975 4823
Fragments arranged by size:
4823 975 502 355
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2255 2772 2891 3044 3353 3478 4716
Size: 277 188 517 119 153 309 125 1238
Cuts at: 4716 5872 1790
Size: 1156 2573
Fragments arranged by size:
2573 1238 1156 517 309 277 188 153
125 119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6655
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4386 5344
Size: 416 53 83 399 24 1850 1119 958
Cuts at: 5344 442
Size: 1753
Fragments arranged by size:
1850 1753 1119 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3555 3904 3555
Size: 349 6306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 2666 3084 3096 3727 3800 5315 1239
Size: 919 508 418 12 631 73 1515 2579
Fragments arranged by size:
2579 1515 919 631 508 418 73 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3899 4780 5645 5943 3899
Size: 881 865 298 4611
Fragments arranged by size:
4611 881 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2671 3543 3678 3814 3875 5318 882
Size: 617 1172 872 135 136 61 1443 2219
Fragments arranged by size:
2219 1443 1172 872 617 136 135 61
BsaAI yAC'GTr
Cuts at: 493 1204 2302 4446 493
Size: 711 1098 2144 2702
Fragments arranged by size:
2702 2144 1098 711
BsaBI GATnn'nnATC
Cuts at: 2591 6649 2591
Size: 4058 2597
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3489 3896 4462 4844
Size: 53 83 186 936 1956 407 566 382
Cuts at: 4844 5479 275
Size: 635 1451
Fragments arranged by size:
1956 1451 936 635 566 407 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2597 5088 5486 5760 5907 6318 2597
Size: 2491 398 274 147 411 2934
Fragments arranged by size:
2934 2491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3725 3755 1178
Size: 30 165 30 2322 30 4078
Fragments arranged by size:
4078 2322 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2071 2819 3392 3574 3797 3824 3829 3832
Size: 885 748 573 182 223 27 5 3
Cuts at: 3832 3848 3851 1186
Size: 16 3 3990
Fragments arranged by size:
3990 885 748 573 223 182 27 16
5 3 3
BseYI C'CCAG_C
Cuts at: 2182 2457 2963 2978 5858 6354 2182
Size: 275 506 15 2880 496 2483
Fragments arranged by size:
2880 2483 506 496 275 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6082
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2145 2199 2220 4105 4866 5894
Size: 275 186 144 54 21 1885 761 1028
Cuts at: 5894 1540
Size: 2301
Fragments arranged by size:
2301 1885 1028 761 275 186 144 54
21
BsiHKAI G_wGCw'C
Cuts at: 2067 2255 2712 2748 3518 4716 4801 5872 2067
Size: 188 457 36 770 1198 85 1071 2850
Fragments arranged by size:
2850 1198 1071 770 457 188 85 36
BsmI GAATG_Cn'
Cuts at: 4166 4259 4166
Size: 93 6562
BsmBI CGTCTCn'nnnn_
Cuts at: 3072 3072
Size: 6655
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2172 2189 2255 2712 2748 2772
Size: 399 277 105 17 66 457 36 24
Cuts at: 2772 2891 3044 3353 3429 3478 3518 3974 4716
Size: 119 153 309 76 49 40 456 742
Cuts at: 4716 4801 5872 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 456 399 309 277
153 119 105 85 76 66 49 40
36 24 17
BspEI T'CCGG_A
Cuts at: 6318 6318
Size: 6655
BspHI T'CATG_A
Cuts at: 4544 6274 4544
Size: 1730 4925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3525 4010 844
Size: 2681 485 3489
Fragments arranged by size:
3489 2681 485
BsrBI CCG'CTC
Cuts at: 1394 1823 3289 3480 4542 1394
Size: 429 1466 191 1062 3507
Fragments arranged by size:
3507 1466 1062 429 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 5150 5324 65
Size: 1183 3902 174 1396
Fragments arranged by size:
3902 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2162 2936 5299 5486 1700
Size: 110 352 774 2363 187 2869
Fragments arranged by size:
2869 2363 774 352 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6655
BssHII G'CGCG_C
Cuts at: 1925 2085 1925
Size: 160 6495
BssSI C'ACGA_G
Cuts at: 3317 3544 4715 5727 3317
Size: 227 1171 1012 4245
Fragments arranged by size:
4245 1171 1012 227
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2250 794
Size: 1456 5199
BstBI TT'CG_AA
Cuts at: 5459 5459
Size: 6655
BstEII G'GTnAC_C
Cuts at: 1184 2278 1184
Size: 1094 5561
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2182
Size: 6655
BstYI r'GATC_y
Cuts at: 1972 3985 4352 4737 4754 6206 6292 6304 6650
Size: 2013 367 385 17 1452 86 12 346
Cuts at: 6650 1972
Size: 1977
Fragments arranged by size:
2013 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2099 2731 2953 2099
Size: 632 222 5801
Fragments arranged by size:
5801 632 222
BtgI C'CryG_G
Cuts at: 513 1065 2661 3036 3483 3584 513
Size: 552 1596 375 447 101 3584
Fragments arranged by size:
3584 1596 552 447 375 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2162 2395 487
Size: 683 56 936 233 4747
Fragments arranged by size:
4747 936 683 233 56
BtsI GCAGTG_nn'
Cuts at: 3154 3175 4171 4965 4985 3154
Size: 21 996 794 20 4824
Fragments arranged by size:
4824 996 794 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6655
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6620
DraI TTT'AAA
Cuts at: 3959 3959
Size: 6655
DraIII CAC_nnn'GTG
Cuts at: 2250 2305 2250
Size: 55 6600
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2219 5662 809
Size: 507 903 3443 1802
Fragments arranged by size:
3443 1802 903 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2160 2196
Size: 54 1425 50 275 18 312 18 36
Cuts at: 2196 2430 2532 3106 3240 3277 3388 4102 4991
Size: 234 102 574 134 37 111 714 889
Cuts at: 4991 6620 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 714 574 312 275 234
134 111 102 54 50 43 37 36
18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2142 2196 4102 1537
Size: 275 330 54 1906 4090
Fragments arranged by size:
4090 1906 330 275 54
EarI CTCTTCn'nnn_
Cuts at: 1353 2318 2792 3837 4585 1353
Size: 965 474 1045 748 3423
Fragments arranged by size:
3423 1045 965 748 474
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5242 5616 5762 387
Size: 1064 138 3653 374 146 1280
Fragments arranged by size:
3653 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2710 2746 2710
Size: 36 6619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2666 3084 3096 3180 3652 3727
Size: 393 526 508 418 12 84 472 75
Cuts at: 3727 3800 4718 5315 6102 6370 1239
Size: 73 918 597 787 268 1524
Fragments arranged by size:
1524 918 787 597 526 508 472 418
393 268 84 75 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3317 3317
Size: 6655
EcoO109I rG'GnC_Cy
Cuts at: 2677 2727 2768 2923 3041 3214 3349 3350 3649
Size: 50 41 155 118 173 135 1 299
Cuts at: 3649 3702 3724 3754 2677
Size: 53 22 30 5578
Fragments arranged by size:
5578 299 173 155 135 118 53 50
41 30 22 1
EcoRI G'AATT_C
Cuts at: 3964 4342 3964
Size: 378 6277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3940 3940
Size: 6655
FspI TGC'GCA
Cuts at: 2516 5161 2516
Size: 2645 4010
FspAI rTGC'GCAy
Cuts at: 2516 2516
Size: 6655
HaeII r_GCGC'y
Cuts at: 1678 2156 3685 5802 1678
Size: 478 1529 2117 2531
Fragments arranged by size:
2531 2117 1529 478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2440 3317 3349 3656
Size: 32 135 32 836 32 877 32 307
Cuts at: 3656 3686 3688 3718 3725 3757 5296 5328 5370
Size: 30 2 30 7 32 1539 32 42
Cuts at: 5370 5402 1373
Size: 32 2626
Fragments arranged by size:
2626 1539 877 836 307 135 42 32
32 32 32 32 32 32 30 30
7 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2440 3085 3999 4245 669
Size: 988 281 502 645 914 246 3079
Fragments arranged by size:
3079 988 914 645 502 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6655
HpaI GTT'AAC
Cuts at: 4245 4245
Size: 6655
KpnI G_GTAC'C
Cuts at: 3061 3980 3061
Size: 919 5736
MfeI C'AATT_G
Cuts at: 4254 4254
Size: 6655
MluI A'CGCG_T
Cuts at: 6531 6531
Size: 6655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 3786 5769 5953 1305
Size: 1008 1473 1983 184 2007
Fragments arranged by size:
2007 1983 1473 1008 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 3108 3242 3390 10
Size: 54 1425 343 702 574 134 148 3275
Fragments arranged by size:
3275 1425 702 574 343 148 134 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 4613 4972 5131 518
Size: 648 1110 2337 359 159 2042
Fragments arranged by size:
2337 2042 1110 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2461 3101 3586 4071 4748 5896 6141 1606
Size: 855 640 485 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 855 677 640 485 485 245
NaeI GCC'GGC
Cuts at: 2164 2938 2164
Size: 774 5881
NcoI C'CATG_G
Cuts at: 513 1065 3036 513
Size: 552 1971 4132
Fragments arranged by size:
4132 1971 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6655
NgoMIV G'CCGG_C
Cuts at: 2162 2936 2162
Size: 774 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6655
NotI GC'GGCC_GC
Cuts at: 4102 4102
Size: 6655
NspI r_CATG'y
Cuts at: 1132 1229 3580 4013 5558 1132
Size: 97 2351 433 1545 2229
Fragments arranged by size:
2351 2229 1545 433 97
PasI CC'CwG_GG
Cuts at: 1348 2560 3408 1348
Size: 1212 848 4595
Fragments arranged by size:
4595 1212 848
PciI A'CATG_T
Cuts at: 5554 5554
Size: 6655
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3414 1108
Size: 2306 4349
PfoI T'CCnGG_A
Cuts at: 3369 3369
Size: 6655
PmeI GTTT'AAAC
Cuts at: 3959 3959
Size: 6655
PmlI CAC'GTG
Cuts at: 2302 2302
Size: 6655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4724 4756 6237 6269 4724
Size: 32 1481 32 5110
Fragments arranged by size:
5110 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2677 2727 3214 3649 3702 3724 3754 2677
Size: 50 487 435 53 22 30 5578
Fragments arranged by size:
5578 487 435 53 50 30 22
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6655
PsiI TTA'TAA
Cuts at: 4225 4225
Size: 6655
PspOMI G'GGCC_C
Cuts at: 2768 3040 3349 3474 2768
Size: 272 309 125 5949
Fragments arranged by size:
5949 309 272 125
PspXI vC'TCGA_Gb
Cuts at: 3625 3625
Size: 6655
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2465 2497 2465
Size: 32 6623
PstI C_TGCA'G
Cuts at: 830 4007 830
Size: 3177 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6655
PvuII CAG'CTG
Cuts at: 2461 3101 2461
Size: 640 6015
SacI G_AGCT'C
Cuts at: 2712 2748 2712
Size: 36 6619
SacII CC_GC'GG
Cuts at: 3587 3587
Size: 6655
SalI G'TCGA_C
Cuts at: 1936 2438 3997 1936
Size: 502 1559 4594
Fragments arranged by size:
4594 1559 502
SanDI GG'GwC_CC
Cuts at: 3649 3724 3649
Size: 75 6580
SapI GCTCTTCn'nnn_
Cuts at: 2792 2792
Size: 6655
SbfI CC_TGCA'GG
Cuts at: 4007 4007
Size: 6655
ScaI AGT'ACT
Cuts at: 4903 6615 4903
Size: 1712 4943
SexAI A'CCwGG_T
Cuts at: 2246 2752 2246
Size: 506 6149
SfcI C'TryA_G
Cuts at: 826 1045 3175 4003 5138 5819 6010 826
Size: 219 2130 828 1135 681 191 1471
Fragments arranged by size:
2130 1471 1135 828 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6655
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6655
SmaI CCC'GGG
Cuts at: 1872 3347 3473 3647 3722 3982 1872
Size: 1475 126 174 75 260 4545
Fragments arranged by size:
4545 1475 260 174 126 75
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 3914 4759 5660 5922 820
Size: 197 926 1682 289 845 901 262 1553
Fragments arranged by size:
1682 1553 926 901 845 289 262 197
SnaBI TAC'GTA
Cuts at: 493 4446 493
Size: 3953 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6655
SphI G_CATG'C
Cuts at: 1132 4013 1132
Size: 2881 3774
SspI AAT'ATT
Cuts at: 5 52 4579 5
Size: 47 4527 2081
Fragments arranged by size:
4527 2081 47
StuI AGG'CCT
Cuts at: 1517 2699 3848 1517
Size: 1182 1149 4324
Fragments arranged by size:
4324 1182 1149
StyI C'CwwG_G
Cuts at: 513 1065 2078 2719 3036 3109 4094 513
Size: 552 1013 641 317 73 985 3074
Fragments arranged by size:
3074 1013 985 641 552 317 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3899 4682 4699 4852 5037 5482 325
Size: 1255 2319 783 17 153 185 445 1498
Fragments arranged by size:
2319 1498 1255 783 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 3219 4901
Size: 274 80 33 51 1443 408 834 1682
Cuts at: 4901 6613 96
Size: 1712 138
Fragments arranged by size:
1712 1682 1443 834 408 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2029 2061 3365 3397 6615 6647 2029
Size: 32 1304 32 3218 32 2037
Fragments arranged by size:
3218 2037 1304 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2216 2284 1316
Size: 900 68 5687
Fragments arranged by size:
5687 900 68
XbaI T'CTAG_A
Cuts at: 3991 3991
Size: 6655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3280 3280
Size: 6655
XhoI C'TCGA_G
Cuts at: 1943 3625 1943
Size: 1682 4973
XmaI C'CCGG_G
Cuts at: 1870 3345 3471 3645 3720 3980 1870
Size: 1475 126 174 75 260 4545
Fragments arranged by size:
4545 1475 260 174 126 75
XmnI GAAnn'nnTTC
Cuts at: 4784 4784
Size: 6655
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4463 276
Size: 53 83 186 936 2929 2468
Fragments arranged by size:
2929 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I EcoRV FseI KasI NarI NruI
NsiI PacI RsrII SfiI SfoI SrfI SwaI