Restriction map for
pFN21AB6167
(Circular) (Six-base) MAPSORT of: pFN21AB6167.seq Check: 6363 from: 1 to: 6652
pFN21AB6167 6652 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2206 3017 3584 2206
Size: 811 567 5274
Fragments arranged by size:
5274 811 567
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4462 278
Size: 53 83 186 936 2926 2468
Fragments arranged by size:
2926 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3995 1937
Size: 2058 4594
Acc65I G'GTAC_C
Cuts at: 3973 3973
Size: 6652
AclI AA'CG_TT
Cuts at: 1563 4779 5152 1563
Size: 3216 373 3063
Fragments arranged by size:
3216 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2916 3524 3881 4715 6099 6367 1632
Size: 641 643 608 357 834 1384 268 1917
Fragments arranged by size:
1917 1384 834 643 641 608 357 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6455
AflIII A'CryG_T
Cuts at: 1203 5551 6528 1203
Size: 4348 977 1327
Fragments arranged by size:
4348 1327 977
AgeI A'CCGG_T
Cuts at: 5483 5483
Size: 6652
AhdI GACnn_n'nnGTC
Cuts at: 5381 5381
Size: 6652
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6652
AlwNI CAG_nnn'CTG
Cuts at: 3013 4395 5967 3013
Size: 1382 1572 3698
Fragments arranged by size:
3698 1572 1382
ApaLI G'TGCA_C
Cuts at: 4709 5865 4709
Size: 1156 5496
ApoI r'AATT_y
Cuts at: 1493 2070 2076 2469 2805 3018 3096 3859 3961
Size: 577 6 393 336 213 78 763 102
Cuts at: 3961 4187 4339 6448 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 763 577 393 336 226 213
152 102 78 6
AseI AT'TA_AT
Cuts at: 160 1032 2447 2928 5206 160
Size: 872 1415 481 2278 1606
Fragments arranged by size:
2278 1606 1415 872 481
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6652
AvaI C'yCGr_G
Cuts at: 1870 1943 3977 1870
Size: 73 2034 4545
Fragments arranged by size:
4545 2034 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3647 3680 3647
Size: 33 6619
BamHI G'GATC_C
Cuts at: 3982 4349 3982
Size: 367 6285
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3973 5428 618
Size: 325 217 627 2186 1455 1842
Fragments arranged by size:
2186 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3971 1391
Size: 2580 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2445 3681 928
Size: 971 546 1236 3899
Fragments arranged by size:
3899 1236 971 546
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4843 4877 1903
Size: 34 2906 34 3678
Fragments arranged by size:
3678 2906 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4544 5754 4544
Size: 1210 5442
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6652
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5263 136
Size: 107 122 71 631 605 3591 1525
Fragments arranged by size:
3591 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3487 3625 6647 3487
Size: 138 3022 3492
Fragments arranged by size:
3492 3022 138
BlpI GC'TnA_GC
Cuts at: 4069 4069
Size: 6652
Bme1580I G_kGCm'C
Cuts at: 1790 4713 5869 1790
Size: 2923 1156 2573
Fragments arranged by size:
2923 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3215 1318
Size: 1897 4755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4383 5341 442
Size: 416 53 83 399 24 2966 958 1753
Fragments arranged by size:
2966 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6652
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2661 2661
Size: 6652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2559 3650 5312 1239
Size: 1320 1091 1662 2579
Fragments arranged by size:
2579 1662 1320 1091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4777 5642 5940 4777
Size: 865 298 5489
Fragments arranged by size:
5489 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5315 882
Size: 617 3816 2219
Fragments arranged by size:
3816 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4443 493
Size: 711 3239 2702
Fragments arranged by size:
3239 2702 711
BsaBI GATnn'nnATC
Cuts at: 6646 6646
Size: 6652
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4459 4841 5476
Size: 53 83 186 936 476 2450 382 635
Cuts at: 5476 275
Size: 1451
Fragments arranged by size:
2450 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5085 5483 5757 5904 6315 5085
Size: 398 274 147 411 5422
Fragments arranged by size:
5422 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6427
Fragments arranged by size:
6427 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6652
BseYI C'CCAG_C
Cuts at: 5855 6351 5855
Size: 496 6156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3237 3594 1163
Size: 573 1501 357 4221
Fragments arranged by size:
4221 1501 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4102 4863 5891 1540
Size: 275 186 2101 761 1028 2301
Fragments arranged by size:
2301 2101 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4713 4798 5869 4713
Size: 85 1071 5496
Fragments arranged by size:
5496 1071 85
BsmI GAATG_Cn'
Cuts at: 4163 4256 4163
Size: 93 6559
BsmBI CGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 6652
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3971 4713 4798 5869 1391
Size: 399 2181 742 85 1071 2174
Fragments arranged by size:
2181 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6315 6315
Size: 6652
BspHI T'CATG_A
Cuts at: 4541 6271 4541
Size: 1730 4922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 3017 3209 3584 4007 844
Size: 1362 811 192 375 423 3489
Fragments arranged by size:
3489 1362 811 423 375 192
BsrBI CCG'CTC
Cuts at: 1394 1823 4539 1394
Size: 429 2716 3507
Fragments arranged by size:
3507 2716 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2185 5147 5321 65
Size: 1183 937 2962 174 1396
Fragments arranged by size:
2962 1396 1183 937 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5296 5483 1700
Size: 110 3486 187 2869
Fragments arranged by size:
3486 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6652
BssSI C'ACGA_G
Cuts at: 3618 4712 5724 3618
Size: 1094 1012 4546
Fragments arranged by size:
4546 1094 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3119 794
Size: 2325 4327
BstBI TT'CG_AA
Cuts at: 5456 5456
Size: 6652
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6652
BstXI CCAn_nnnn'nTGG
Cuts at: 3209 3209
Size: 6652
BstYI r'GATC_y
Cuts at: 1972 3487 3498 3625 3982 4349 4734 4751 6203
Size: 1515 11 127 357 367 385 17 1452
Cuts at: 6203 6289 6301 6647 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1515 1452 385 367 357 346 127
86 17 12 11
BtgI C'CryG_G
Cuts at: 513 1065 2004 2225 3186 513
Size: 552 939 221 961 3979
Fragments arranged by size:
3979 961 939 552 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5913
Fragments arranged by size:
5913 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 2668 4168 4962 4982 2153
Size: 515 1500 794 20 3823
Fragments arranged by size:
3823 1500 794 515 20
ClaI AT'CG_AT
Cuts at: 1554 3942 1554
Size: 2388 4264
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6617
DraI TTT'AAA
Cuts at: 2266 2457 3956 2266
Size: 191 1499 4962
Fragments arranged by size:
4962 1499 191
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3219 5659 809
Size: 507 1903 2440 1802
Fragments arranged by size:
2440 1903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4099 4988 6617
Size: 54 1425 50 275 18 2269 889 1629
Cuts at: 6617 8
Size: 43
Fragments arranged by size:
2269 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4099 1537
Size: 275 2287 4090
Fragments arranged by size:
4090 2287 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4582 1353
Size: 3229 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5239 5613 5759 387
Size: 1064 138 3650 374 146 1280
Fragments arranged by size:
3650 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2559 2916 3524 3650 3881 4715
Size: 393 641 286 357 608 126 231 834
Cuts at: 4715 5312 6099 6367 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 834 787 641 608 597 393 357
286 268 231 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2349 3506 2349
Size: 1157 5495
EcoO109I rG'GnC_Cy
Cuts at: 3722 3722
Size: 6652
EcoRI G'AATT_C
Cuts at: 3018 3961 4339 3018
Size: 943 378 5331
Fragments arranged by size:
5331 943 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3752 3752
Size: 6652
FspI TGC'GCA
Cuts at: 5158 5158
Size: 6652
HaeII r_GCGC'y
Cuts at: 1678 5799 1678
Size: 4121 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2782 2814 3320 3352 3477
Size: 32 135 32 1210 32 506 32 125
Cuts at: 3477 3509 5293 5325 5367 5399 1373
Size: 32 1784 32 42 32 2626
Fragments arranged by size:
2626 1784 1210 506 135 125 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3996 4242 669
Size: 988 281 2058 246 3079
Fragments arranged by size:
3079 2058 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3320 748
Size: 2572 4080
HpaI GTT'AAC
Cuts at: 4242 4242
Size: 6652
KpnI G_GTAC'C
Cuts at: 3977 3977
Size: 6652
MfeI C'AATT_G
Cuts at: 4251 4251
Size: 6652
MluI A'CGCG_T
Cuts at: 6528 6528
Size: 6652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5766 5950 1305
Size: 4461 184 2007
Fragments arranged by size:
4461 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4830
Fragments arranged by size:
4830 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3027 3390 4610 4969 5128 518
Size: 648 1861 363 1220 359 159 2042
Fragments arranged by size:
2042 1861 1220 648 363 359 159
MspA1I CmG'CkG
Cuts at: 1606 4068 4745 5893 6138 1606
Size: 2462 677 1148 245 2120
Fragments arranged by size:
2462 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2225 3186 513
Size: 552 939 221 961 3979
Fragments arranged by size:
3979 961 939 552 221
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6652
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6652
NotI GC'GGCC_GC
Cuts at: 4099 4099
Size: 6652
NspI r_CATG'y
Cuts at: 1132 1229 2063 2513 3417 3758 3895 4010 5555
Size: 97 834 450 904 341 137 115 1545
Cuts at: 5555 1132
Size: 2229
Fragments arranged by size:
2229 1545 904 834 450 341 137 115
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6652
PciI A'CATG_T
Cuts at: 5551 5551
Size: 6652
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2231 2449 1108
Size: 1123 218 5311
Fragments arranged by size:
5311 1123 218
PmeI GTTT'AAAC
Cuts at: 3956 3956
Size: 6652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4721 4753 6234 6266 4721
Size: 32 1481 32 5107
Fragments arranged by size:
5107 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3722 3722
Size: 6652
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6652
PsiI TTA'TAA
Cuts at: 2709 4222 2709
Size: 1513 5139
PstI C_TGCA'G
Cuts at: 830 3485 4004 830
Size: 2655 519 3478
Fragments arranged by size:
3478 2655 519
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6652
SalI G'TCGA_C
Cuts at: 1936 3994 1936
Size: 2058 4594
SbfI CC_TGCA'GG
Cuts at: 4004 4004
Size: 6652
ScaI AGT'ACT
Cuts at: 3479 4900 6612 3479
Size: 1421 1712 3519
Fragments arranged by size:
3519 1712 1421
SfcI C'TryA_G
Cuts at: 826 1045 2346 2674 3481 3679 4000 5135 5816
Size: 219 1301 328 807 198 321 1135 681
Cuts at: 5816 6007 826
Size: 191 1471
Fragments arranged by size:
1471 1301 1135 807 681 328 321 219
198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6652
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6652
SmaI CCC'GGG
Cuts at: 1872 3979 1872
Size: 2107 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4756 5657 5919 820
Size: 197 926 2813 901 262 1553
Fragments arranged by size:
2813 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4443 493
Size: 3950 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6652
SphI G_CATG'C
Cuts at: 1132 4010 1132
Size: 2878 3774
SspI AAT'ATT
Cuts at: 5 52 4576 5
Size: 47 4524 2081
Fragments arranged by size:
4524 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6652
StyI C'CwwG_G
Cuts at: 513 1065 2004 2225 3186 4091 513
Size: 552 939 221 961 905 3074
Fragments arranged by size:
3074 961 939 905 552 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4679 4696 4849 5034 5479 325
Size: 1255 3099 17 153 185 445 1498
Fragments arranged by size:
3099 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3252 3477 4898
Size: 274 80 33 51 1443 1275 225 1421
Cuts at: 4898 6610 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1421 1275 274 225 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6612 6644 6612
Size: 32 6620
Tth111I GACn'n_nGTC
Cuts at: 1316 3778 1316
Size: 2462 4190
XbaI T'CTAG_A
Cuts at: 3988 3988
Size: 6652
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6652
XmaI C'CCGG_G
Cuts at: 1870 3977 1870
Size: 2107 4545
XmnI GAAnn'nnTTC
Cuts at: 2220 3283 3794 4781 2220
Size: 1063 511 987 4091
Fragments arranged by size:
4091 1063 987 511
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4460 276
Size: 53 83 186 936 2926 2468
Fragments arranged by size:
2926 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI