Restriction map for
pFN21AB6165
(Circular) (Six-base) MAPSORT of: pFN21AB6165.seq Check: 5472 from: 1 to: 6649
pFN21AB6165 6649 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3776 3776
Size: 6649
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2037 2406 4459 278
Size: 53 83 186 936 501 369 2053 2468
Fragments arranged by size:
2468 2053 936 501 369 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3992 1937
Size: 2055 4594
Acc65I G'GTAC_C
Cuts at: 3970 3970
Size: 6649
AclI AA'CG_TT
Cuts at: 1563 4776 5149 1563
Size: 3213 373 3063
Fragments arranged by size:
3213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2125 2260 4712 6096 6364 1632
Size: 493 135 2452 1384 268 1917
Fragments arranged by size:
2452 1917 1384 493 268 135
AflII C'TTAA_G
Cuts at: 820 1017 2855 820
Size: 197 1838 4614
Fragments arranged by size:
4614 1838 197
AflIII A'CryG_T
Cuts at: 1203 5548 6525 1203
Size: 4345 977 1327
Fragments arranged by size:
4345 1327 977
AgeI A'CCGG_T
Cuts at: 5480 5480
Size: 6649
AhdI GACnn_n'nnGTC
Cuts at: 5378 5378
Size: 6649
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6649
AlwNI CAG_nnn'CTG
Cuts at: 4392 5964 4392
Size: 1572 5077
ApaI G_GGCC'C
Cuts at: 2051 2229 2274 3046 3454 3759 2051
Size: 178 45 772 408 305 4941
Fragments arranged by size:
4941 772 408 305 178 45
ApaLI G'TGCA_C
Cuts at: 4706 5862 4706
Size: 1156 5493
ApoI r'AATT_y
Cuts at: 1493 2953 3958 4184 4336 6445 1493
Size: 1460 1005 226 152 2109 1697
Fragments arranged by size:
2109 1697 1460 1005 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5203 160
Size: 872 4171 1606
Fragments arranged by size:
4171 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
AvaI C'yCGr_G
Cuts at: 1870 1943 2228 3802 3974 1870
Size: 73 285 1574 172 4545
Fragments arranged by size:
4545 1574 285 172 73
BamHI G'GATC_C
Cuts at: 3979 4346 3979
Size: 367 6282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2773 2796 3244 3435
Size: 325 217 627 305 681 23 448 191
Cuts at: 3435 3461 3970 5425 618
Size: 26 509 1455 1842
Fragments arranged by size:
1842 1455 681 627 509 448 325 305
217 191 26 23
BanII G_rGCy'C
Cuts at: 1391 2051 2229 2274 2556 2747 3046 3185 3454
Size: 660 178 45 282 191 299 139 269
Cuts at: 3454 3759 3879 3968 1391
Size: 305 120 89 4072
Fragments arranged by size:
4072 660 305 299 282 269 191 178
139 120 89 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2398 928
Size: 971 499 5179
Fragments arranged by size:
5179 971 499
BbvCI CC'TCA_GC
Cuts at: 3917 3917
Size: 6649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4840 4874 1903
Size: 34 2903 34 3678
Fragments arranged by size:
3678 2903 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2310 4541 5751 2310
Size: 2231 1210 3208
Fragments arranged by size:
3208 2231 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2106 2256 2278
Size: 107 122 71 631 605 434 150 22
Cuts at: 2278 3211 3433 5260 136
Size: 933 222 1827 1525
Fragments arranged by size:
1827 1525 933 631 605 434 222 150
122 107 71 22
BglII A'GATC_T
Cuts at: 2892 6644 2892
Size: 3752 2897
BlpI GC'TnA_GC
Cuts at: 4066 4066
Size: 6649
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2229 2274 2799 3046 3342 3438 3454
Size: 261 178 45 525 247 296 96 16
Cuts at: 3454 3759 3826 4710 5866 1790
Size: 305 67 884 1156 2573
Fragments arranged by size:
2573 1156 884 525 305 296 261 247
178 96 67 45 16
BmgBI CAC'GTC
Cuts at: 1318 2497 1318
Size: 1179 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2483 2612 2880
Size: 416 53 83 399 24 1066 129 268
Cuts at: 2880 3836 4380 5338 442
Size: 956 544 958 1753
Fragments arranged by size:
1753 1066 958 956 544 416 399 268
129 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2157 2489 2645 3276 3893 5309 1239
Size: 842 76 332 156 631 617 1416 2579
Fragments arranged by size:
2579 1416 842 631 617 332 156 76
Bpu10I CC'TnA_GC
Cuts at: 3223 3917 3223
Size: 694 5955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 3461 4774 5639 5937 2336
Size: 1125 1313 865 298 3048
Fragments arranged by size:
3048 1313 1125 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2414 3567 5312 882
Size: 617 915 1153 1745 2219
Fragments arranged by size:
2219 1745 1153 915 617
BsaAI yAC'GTr
Cuts at: 493 1204 4440 493
Size: 711 3236 2702
Fragments arranged by size:
3236 2702 711
BsaBI GATnn'nnATC
Cuts at: 6643 6643
Size: 6649
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2403 4456 4838
Size: 53 83 186 936 501 369 2053 382
Cuts at: 4838 5473 275
Size: 635 1451
Fragments arranged by size:
2053 1451 936 635 501 382 369 186
83 53
BsaWI w'CCGG_w
Cuts at: 5082 5480 5754 5901 6312 5082
Size: 398 274 147 411 5419
Fragments arranged by size:
5419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2085 2115 2666 2696 2729
Size: 30 165 30 682 30 551 30 33
Cuts at: 2729 2759 3332 3362 1178
Size: 30 573 30 4465
Fragments arranged by size:
4465 682 573 551 165 33 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2495 2671 2737 3092 1186
Size: 1147 162 176 66 355 4743
Fragments arranged by size:
4743 1147 355 176 162 66
BseYI C'CCAG_C
Cuts at: 2251 2564 2636 2685 3178 3250 3286 3453 3500
Size: 313 72 49 493 72 36 167 47
Cuts at: 3500 3535 3726 3923 5852 6348 2251
Size: 35 191 197 1929 496 2552
Fragments arranged by size:
2552 1929 496 493 313 197 191 167
72 72 49 47 36 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3257 3821 1163
Size: 573 1521 564 3991
Fragments arranged by size:
3991 1521 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4099 4860 5888 1540
Size: 275 186 2098 761 1028 2301
Fragments arranged by size:
2301 2098 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2916 4710 4795 5866 2916
Size: 1794 85 1071 3699
Fragments arranged by size:
3699 1794 1071 85
BsmI GAATG_Cn'
Cuts at: 4160 4253 4160
Size: 93 6556
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2229 2274 2556 2747 2799 2916
Size: 399 261 178 45 282 191 52 117
Cuts at: 2916 3046 3185 3342 3438 3454 3759 3826 3879
Size: 130 139 157 96 16 305 67 53
Cuts at: 3879 3968 4710 4795 5866 1391
Size: 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 305 282 261 191
178 157 139 130 117 96 89 85
67 53 52 45 16
BspEI T'CCGG_A
Cuts at: 6312 6312
Size: 6649
BspHI T'CATG_A
Cuts at: 4538 6268 4538
Size: 1730 4919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3776 3843 4004 844
Size: 2932 67 161 3489
Fragments arranged by size:
3489 2932 161 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4536 1394
Size: 429 2713 3507
Fragments arranged by size:
3507 2713 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2211 3108 5144 5318 65
Size: 1183 963 897 2036 174 1396
Fragments arranged by size:
2036 1396 1183 963 897 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2135 2255 5293 5480 1700
Size: 110 325 120 3038 187 2869
Fragments arranged by size:
3038 2869 325 187 120 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6649
BssSI C'ACGA_G
Cuts at: 4709 5721 4709
Size: 1012 5637
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3033 794
Size: 2239 4410
BstBI TT'CG_AA
Cuts at: 5453 5453
Size: 6649
BstEII G'GTnAC_C
Cuts at: 1184 3688 3830 1184
Size: 2504 142 4003
Fragments arranged by size:
4003 2504 142
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 2576 3520 3937 2059
Size: 517 944 417 4771
Fragments arranged by size:
4771 944 517 417
BstYI r'GATC_y
Cuts at: 1972 2484 2892 3103 3979 4346 4731 4748 6200
Size: 512 408 211 876 367 385 17 1452
Cuts at: 6200 6286 6298 6644 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 876 512 408 385 367 346
211 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3888 3936 513
Size: 552 939 1884 48 3226
Fragments arranged by size:
3226 1884 939 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5910
Fragments arranged by size:
5910 683 56
BtsI GCAGTG_nn'
Cuts at: 2189 2245 3269 3739 3784 3814 4165 4959 4979
Size: 56 1024 470 45 30 351 794 20
Cuts at: 4979 2189
Size: 3859
Fragments arranged by size:
3859 1024 794 470 351 56 45 30
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6614
DraI TTT'AAA
Cuts at: 3953 3953
Size: 6649
DraIII CAC_nnn'GTG
Cuts at: 3830 3830
Size: 6649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5656 809
Size: 507 4340 1802
Fragments arranged by size:
4340 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3054 3129 3522
Size: 54 1425 50 275 18 1224 75 393
Cuts at: 3522 4096 4985 6614 8
Size: 574 889 1629 43
Fragments arranged by size:
1629 1425 1224 889 574 393 275 75
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4096 1537
Size: 275 2284 4090
Fragments arranged by size:
4090 2284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2813 3089 3135 4579 1353
Size: 1460 276 46 1444 3423
Fragments arranged by size:
3423 1460 1444 276 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2516 3192 5236 5610 5756 387
Size: 1064 138 927 676 2044 374 146 1280
Fragments arranged by size:
2044 1280 1064 927 676 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2125 2157 2260 2489 2645 3276
Size: 393 449 44 32 103 229 156 631
Cuts at: 3276 3893 4712 5309 6096 6364 1239
Size: 617 819 597 787 268 1524
Fragments arranged by size:
1524 819 787 631 617 597 449 393
268 229 156 103 44 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 6649
EcoO109I rG'GnC_Cy
Cuts at: 2048 2080 2225 2271 3043 3227 3296 3325 3756
Size: 32 145 46 772 184 69 29 431
Cuts at: 3756 3902 2048
Size: 146 4795
Fragments arranged by size:
4795 772 431 184 146 145 69 46
32 29
EcoRI G'AATT_C
Cuts at: 2953 3958 4336 2953
Size: 1005 378 5266
Fragments arranged by size:
5266 1005 378
FspI TGC'GCA
Cuts at: 3119 5155 3119
Size: 2036 4613
HaeII r_GCGC'y
Cuts at: 1678 2074 5796 1678
Size: 396 3722 2531
Fragments arranged by size:
3722 2531 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2993 3025 3088 3120 5290
Size: 32 135 32 1421 32 63 32 2170
Cuts at: 5290 5322 5364 5396 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2170 1421 135 63 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3993 4239 669
Size: 988 281 2055 246 3079
Fragments arranged by size:
3079 2055 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6649
HpaI GTT'AAC
Cuts at: 4239 4239
Size: 6649
KpnI G_GTAC'C
Cuts at: 3974 3974
Size: 6649
MfeI C'AATT_G
Cuts at: 4248 4248
Size: 6649
MluI A'CGCG_T
Cuts at: 6525 6525
Size: 6649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 5763 5947 1305
Size: 882 3576 184 2007
Fragments arranged by size:
3576 2007 882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3131 10
Size: 54 1425 343 1299 3528
Fragments arranged by size:
3528 1425 1299 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2846 3113 4607 4966 5125 518
Size: 648 1680 267 1494 359 159 2042
Fragments arranged by size:
2042 1680 1494 648 359 267 159
MspA1I CmG'CkG
Cuts at: 1606 2145 2467 3254 4065 4742 5890 6135 1606
Size: 539 322 787 811 677 1148 245 2120
Fragments arranged by size:
2120 1148 811 787 677 539 322 245
NaeI GCC'GGC
Cuts at: 2137 2257 2137
Size: 120 6529
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5158
Fragments arranged by size:
5158 939 552
NdeI CA'TA_TG
Cuts at: 387 2670 387
Size: 2283 4366
NgoMIV G'CCGG_C
Cuts at: 2135 2255 2135
Size: 120 6529
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6649
NotI GC'GGCC_GC
Cuts at: 4096 4096
Size: 6649
NspI r_CATG'y
Cuts at: 1132 1229 2135 4007 5552 1132
Size: 97 906 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 906 97
PasI CC'CwG_GG
Cuts at: 1348 2267 2339 2459 3209 3752 1348
Size: 919 72 120 750 543 4245
Fragments arranged by size:
4245 919 750 543 120 72
PciI A'CATG_T
Cuts at: 5548 5548
Size: 6649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2590 2717 2770 1108
Size: 1482 127 53 4987
Fragments arranged by size:
4987 1482 127 53
PfoI T'CCnGG_A
Cuts at: 3397 3397
Size: 6649
PmeI GTTT'AAAC
Cuts at: 3953 3953
Size: 6649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 6231 6263 4718
Size: 32 1481 32 5104
Fragments arranged by size:
5104 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 3325 2080
Size: 1245 5404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6649
PsiI TTA'TAA
Cuts at: 4219 4219
Size: 6649
PspOMI G'GGCC_C
Cuts at: 2047 2225 2270 3042 3450 3755 2047
Size: 178 45 772 408 305 4941
Fragments arranged by size:
4941 772 408 305 178 45
PstI C_TGCA'G
Cuts at: 830 3324 3901 4001 830
Size: 2494 577 100 3478
Fragments arranged by size:
3478 2494 577 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6649
PvuII CAG'CTG
Cuts at: 2145 3254 2145
Size: 1109 5540
SalI G'TCGA_C
Cuts at: 1936 3991 1936
Size: 2055 4594
SapI GCTCTTCn'nnn_
Cuts at: 3135 3135
Size: 6649
SbfI CC_TGCA'GG
Cuts at: 3324 3901 4001 3324
Size: 577 100 5972
Fragments arranged by size:
5972 577 100
ScaI AGT'ACT
Cuts at: 2705 4897 6609 2705
Size: 2192 1712 2745
Fragments arranged by size:
2745 2192 1712
SfcI C'TryA_G
Cuts at: 826 1045 2830 3320 3897 3997 5132 5813 6004
Size: 219 1785 490 577 100 1135 681 191
Cuts at: 6004 826
Size: 1471
Fragments arranged by size:
1785 1471 1135 681 577 490 219 191
100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6649
SmaI CCC'GGG
Cuts at: 1872 3804 3976 1872
Size: 1932 172 4545
Fragments arranged by size:
4545 1932 172
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 2855 3440 4753 5654 5916
Size: 197 926 408 504 585 1313 901 262
Cuts at: 5916 820
Size: 1553
Fragments arranged by size:
1553 1313 926 901 585 504 408 262
197
SnaBI TAC'GTA
Cuts at: 493 4440 493
Size: 3947 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6649
SphI G_CATG'C
Cuts at: 1132 2135 4007 1132
Size: 1003 1872 3774
Fragments arranged by size:
3774 1872 1003
SspI AAT'ATT
Cuts at: 5 52 4573 5
Size: 47 4521 2081
Fragments arranged by size:
4521 2081 47
StuI AGG'CCT
Cuts at: 1517 2474 2513 2540 3387 1517
Size: 957 39 27 847 4779
Fragments arranged by size:
4779 957 847 39 27
StyI C'CwwG_G
Cuts at: 513 1065 2004 2074 3577 4088 513
Size: 552 939 70 1503 511 3074
Fragments arranged by size:
3074 1503 939 552 511 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3300 3514 3818 4676 4693 4846 5031
Size: 1255 1720 214 304 858 17 153 185
Cuts at: 5031 5476 325
Size: 445 1498
Fragments arranged by size:
1720 1498 1255 858 445 304 214 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 2703 4895
Size: 274 80 33 51 1443 696 30 2192
Cuts at: 4895 6607 96
Size: 1712 138
Fragments arranged by size:
2192 1712 1443 696 274 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2957 2989 3332 3364 6609 6641 2085
Size: 32 840 32 343 32 3245 32 2093
Fragments arranged by size:
3245 2093 840 343 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6649
XbaI T'CTAG_A
Cuts at: 3985 3985
Size: 6649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 3238 2737
Size: 501 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6649
XmaI C'CCGG_G
Cuts at: 1870 3802 3974 1870
Size: 1932 172 4545
Fragments arranged by size:
4545 1932 172
XmnI GAAnn'nnTTC
Cuts at: 4778 4778
Size: 6649
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2035 2404 4457 276
Size: 53 83 186 936 501 369 2053 2468
Fragments arranged by size:
2468 2053 936 501 369 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI