Restriction map for
pFN21AB6164
(Circular) (Six-base) MAPSORT of: pFN21AB6164.seq Check: 6319 from: 1 to: 6511
pFN21AB6164 6511 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3174 4321 278
Size: 53 83 186 936 1638 1147 2468
Fragments arranged by size:
2468 1638 1147 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3018 3854 1937
Size: 1081 836 4594
Fragments arranged by size:
4594 1081 836
Acc65I G'GTAC_C
Cuts at: 3832 3832
Size: 6511
AclI AA'CG_TT
Cuts at: 1563 4638 5011 1563
Size: 3075 373 3063
Fragments arranged by size:
3075 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3434 3665 4574 5958 6226 1632
Size: 1802 231 909 1384 268 1917
Fragments arranged by size:
1917 1802 1384 909 268 231
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6314
AflIII A'CryG_T
Cuts at: 1203 3738 5410 6387 1203
Size: 2535 1672 977 1327
Fragments arranged by size:
2535 1672 1327 977
AgeI A'CCGG_T
Cuts at: 5342 5342
Size: 6511
AhdI GACnn_n'nnGTC
Cuts at: 2398 5240 2398
Size: 2842 3669
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6511
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 3198 3230 3125
Size: 32 41 32 6406
Fragments arranged by size:
6406 41 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2579 3442 3643 4254 5826 2579
Size: 863 201 611 1572 3264
Fragments arranged by size:
3264 1572 863 611 201
ApaI G_GGCC'C
Cuts at: 2238 2238
Size: 6511
ApaLI G'TGCA_C
Cuts at: 4568 5724 4568
Size: 1156 5355
ApoI r'AATT_y
Cuts at: 1493 2423 3820 4046 4198 6307 1493
Size: 930 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 930 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5065 160
Size: 872 4033 1606
Fragments arranged by size:
4033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2760 3324 3836 1870
Size: 73 131 686 564 512 4545
Fragments arranged by size:
4545 686 564 512 131 73
BamHI G'GATC_C
Cuts at: 2755 3841 4208 2755
Size: 1086 367 5058
Fragments arranged by size:
5058 1086 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3832 5287 618
Size: 325 217 627 2045 1455 1842
Fragments arranged by size:
2045 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2238 2607 2820 2904 3577 3830 1391
Size: 646 201 369 213 84 673 253 4072
Fragments arranged by size:
4072 673 646 369 253 213 201 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5540
BbvCI CC'TCA_GC
Cuts at: 2285 3027 3627 2285
Size: 742 600 5169
Fragments arranged by size:
5169 742 600
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4702 4736 1903
Size: 34 50 34 2681 34 3678
Fragments arranged by size:
3678 2681 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3570 4403 5613 3570
Size: 833 1210 4468
Fragments arranged by size:
4468 1210 833
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6511
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5122 136
Size: 107 122 71 631 605 3450 1525
Fragments arranged by size:
3450 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6506 6506
Size: 6511
BlpI GC'TnA_GC
Cuts at: 2210 2898 3928 2210
Size: 688 1030 4793
Fragments arranged by size:
4793 1030 688
Bme1580I G_kGCm'C
Cuts at: 1790 2238 4572 5728 1790
Size: 448 2334 1156 2573
Fragments arranged by size:
2573 2334 1156 448
BmgBI CAC'GTC
Cuts at: 1318 3739 1318
Size: 2421 4090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2143 3550 4242
Size: 416 53 83 399 24 726 1407 692
Cuts at: 4242 5200 442
Size: 958 1753
Fragments arranged by size:
1753 1407 958 726 692 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 2927 3077 3176 3779 5171 1239
Size: 1379 309 150 99 603 1392 2579
Fragments arranged by size:
2579 1392 1379 603 309 150 99
Bpu10I CC'TnA_GC
Cuts at: 2285 3027 3627 2285
Size: 742 600 5169
Fragments arranged by size:
5169 742 600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2687 2762 3086 4636 5501 5799 2687
Size: 75 324 1550 865 298 3399
Fragments arranged by size:
3399 1550 865 324 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3608 5174 882
Size: 617 2109 1566 2219
Fragments arranged by size:
2219 2109 1566 617
BsaAI yAC'GTr
Cuts at: 493 1204 4302 493
Size: 711 3098 2702
Fragments arranged by size:
3098 2702 711
BsaBI GATnn'nnATC
Cuts at: 6505 6505
Size: 6511
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3171 4318 4700 5335
Size: 53 83 186 936 1638 1147 382 635
Cuts at: 5335 275
Size: 1451
Fragments arranged by size:
1638 1451 1147 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2544 4944 5342 5616 5763 6174 2544
Size: 2400 398 274 147 411 2881
Fragments arranged by size:
2881 2400 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 2715 2745 3125
Size: 30 165 30 936 30 346 30 380
Cuts at: 3125 3155 3347 3377 1178
Size: 30 192 30 4312
Fragments arranged by size:
4312 936 380 346 192 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2260 2363 3173 3548 1186
Size: 934 140 103 810 375 4149
Fragments arranged by size:
4149 934 810 375 140 103
BseYI C'CCAG_C
Cuts at: 2112 2124 2130 2136 2180 5714 6210 2112
Size: 12 6 6 44 3534 496 2413
Fragments arranged by size:
3534 2413 496 44 12 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3333 3439 1163
Size: 573 1597 106 4235
Fragments arranged by size:
4235 1597 573 106
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3961 4722 5750 1540
Size: 275 186 1960 761 1028 2301
Fragments arranged by size:
2301 1960 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2904 2961 3271 4572 4657 5728 2904
Size: 57 310 1301 85 1071 3687
Fragments arranged by size:
3687 1301 1071 310 85 57
BsmI GAATG_Cn'
Cuts at: 4022 4115 4022
Size: 93 6418
BsmBI CGTCTCn'nnnn_
Cuts at: 2867 2867
Size: 6511
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2238 2607 2820 2904 2961 3271
Size: 399 247 201 369 213 84 57 310
Cuts at: 3271 3577 3830 4572 4657 5728 1391
Size: 306 253 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 310 306 253
247 213 201 85 84 57
BspEI T'CCGG_A
Cuts at: 2544 6174 2544
Size: 3630 2881
BspHI T'CATG_A
Cuts at: 3297 4400 6130 3297
Size: 1103 1730 3678
Fragments arranged by size:
3678 1730 1103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3866 844
Size: 3022 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2344 2767 4398 1394
Size: 429 521 423 1631 3507
Fragments arranged by size:
3507 1631 521 429 423
BsrDI GCAATG_nn'
Cuts at: 65 1248 5006 5180 65
Size: 1183 3758 174 1396
Fragments arranged by size:
3758 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3082 3489 5155 5342 1700
Size: 110 1272 407 1666 187 2869
Fragments arranged by size:
2869 1666 1272 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 3741 96
Size: 3645 2866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6511
BssSI C'ACGA_G
Cuts at: 2639 4571 5583 2639
Size: 1932 1012 3567
Fragments arranged by size:
3567 1932 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3266 794
Size: 2472 4039
BstBI TT'CG_AA
Cuts at: 5315 5315
Size: 6511
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6511
BstYI r'GATC_y
Cuts at: 1972 2755 2910 3841 4208 4593 4610 6062 6148
Size: 783 155 931 367 385 17 1452 86
Cuts at: 6148 6160 6506 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 931 783 385 367 346 155
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2511 2511
Size: 6511
BtgI C'CryG_G
Cuts at: 513 1065 3391 513
Size: 552 2326 3633
Fragments arranged by size:
3633 2326 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2777 487
Size: 683 56 1551 4221
Fragments arranged by size:
4221 1551 683 56
BtsI GCAGTG_nn'
Cuts at: 3584 4027 4821 4841 3584
Size: 443 794 20 5254
Fragments arranged by size:
5254 794 443 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2068 2103 529
Size: 35 1504 35 4937
Fragments arranged by size:
4937 1504 35 35
DraI TTT'AAA
Cuts at: 3815 3815
Size: 6511
DraIII CAC_nnn'GTG
Cuts at: 2414 2414
Size: 6511
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5518 809
Size: 507 4202 1802
Fragments arranged by size:
4202 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2624 3225 3958
Size: 54 1425 50 275 18 794 601 733
Cuts at: 3958 4847 6476 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 794 733 601 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3958 1537
Size: 275 2146 4090
Fragments arranged by size:
4090 2146 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2546 2949 3218 3507 3641 3774 4441 1353
Size: 1193 403 269 289 134 133 667 3423
Fragments arranged by size:
3423 1193 667 403 289 269 134 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3206 3791 5098 5472 5618 387
Size: 1064 138 1617 585 1307 374 146 1280
Fragments arranged by size:
1617 1307 1280 1064 585 374 146 138
EcoICRI GAG'CTC
Cuts at: 2902 2902
Size: 6511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 2927 3077 3176 3434 3665 3779
Size: 393 986 309 150 99 258 231 114
Cuts at: 3779 4574 5171 5958 6226 1239
Size: 795 597 787 268 1524
Fragments arranged by size:
1524 986 795 787 597 393 309 268
258 231 150 114 99
EcoNI CCTnn'n_nnAGG
Cuts at: 2996 3059 2996
Size: 63 6448
EcoO109I rG'GnC_Cy
Cuts at: 2234 2235 2234
Size: 1 6510
EcoRI G'AATT_C
Cuts at: 3820 4198 3820
Size: 378 6133
FspI TGC'GCA
Cuts at: 5017 5017
Size: 6511
HaeII r_GCGC'y
Cuts at: 1678 5658 1678
Size: 3980 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 2866 2898 5152
Size: 32 135 32 812 32 450 32 2254
Cuts at: 5152 5184 5226 5258 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2254 812 450 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3855 4101 669
Size: 988 281 1917 246 3079
Fragments arranged by size:
3079 1917 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2309 748
Size: 1561 4950
HpaI GTT'AAC
Cuts at: 4101 4101
Size: 6511
KpnI G_GTAC'C
Cuts at: 3836 3836
Size: 6511
MfeI C'AATT_G
Cuts at: 4110 4110
Size: 6511
MluI A'CGCG_T
Cuts at: 6387 6387
Size: 6511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 3702 5625 5809 1305
Size: 888 1509 1923 184 2007
Fragments arranged by size:
2007 1923 1509 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2626 3227 10
Size: 54 1425 343 794 601 3294
Fragments arranged by size:
3294 1425 794 601 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 4469 4828 4987 518
Size: 648 1315 1988 359 159 2042
Fragments arranged by size:
2042 1988 1315 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3031 3223 3626 3927 4604 5752 5997 1606
Size: 1425 192 403 301 677 1148 245 2120
Fragments arranged by size:
2120 1425 1148 677 403 301 245 192
NcoI C'CATG_G
Cuts at: 513 1065 3391 513
Size: 552 2326 3633
Fragments arranged by size:
3633 2326 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6511
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6511
NotI GC'GGCC_GC
Cuts at: 3958 3958
Size: 6511
NspI r_CATG'y
Cuts at: 1132 1229 3869 5414 1132
Size: 97 2640 1545 2229
Fragments arranged by size:
2640 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6511
PciI A'CATG_T
Cuts at: 5410 5410
Size: 6511
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6511
PfoI T'CCnGG_A
Cuts at: 2904 3054 2904
Size: 150 6361
PmeI GTTT'AAAC
Cuts at: 3815 3815
Size: 6511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 4580 4612 6093 6125 3125
Size: 32 1423 32 1481 32 3511
Fragments arranged by size:
3511 1481 1423 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6511
PsiI TTA'TAA
Cuts at: 4081 4081
Size: 6511
PspOMI G'GGCC_C
Cuts at: 2234 2234
Size: 6511
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3275 3307 3551 3583 3275
Size: 32 244 32 6203
Fragments arranged by size:
6203 244 32 32
PstI C_TGCA'G
Cuts at: 830 2307 3863 830
Size: 1477 1556 3478
Fragments arranged by size:
3478 1556 1477
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6511
PvuII CAG'CTG
Cuts at: 3031 3223 3626 3031
Size: 192 403 5916
Fragments arranged by size:
5916 403 192
SacI G_AGCT'C
Cuts at: 2904 2904
Size: 6511
SalI G'TCGA_C
Cuts at: 1936 3853 1936
Size: 1917 4594
SapI GCTCTTCn'nnn_
Cuts at: 2949 2949
Size: 6511
SbfI CC_TGCA'GG
Cuts at: 3863 3863
Size: 6511
ScaI AGT'ACT
Cuts at: 2225 4759 6471 2225
Size: 2534 1712 2265
Fragments arranged by size:
2534 2265 1712
SfcI C'TryA_G
Cuts at: 826 1045 2303 2857 3355 3859 4994 5675 5866
Size: 219 1258 554 498 504 1135 681 191
Cuts at: 5866 826
Size: 1471
Fragments arranged by size:
1471 1258 1135 681 554 504 498 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6511
SmaI CCC'GGG
Cuts at: 1872 3838 1872
Size: 1966 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 2760 2777 3101 4615 5516
Size: 197 926 723 94 17 324 1514 901
Cuts at: 5516 5778 820
Size: 262 1553
Fragments arranged by size:
1553 1514 926 901 723 324 262 197
94 17
SnaBI TAC'GTA
Cuts at: 493 4302 493
Size: 3809 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6511
SphI G_CATG'C
Cuts at: 1132 3869 1132
Size: 2737 3774
SspI AAT'ATT
Cuts at: 5 52 4435 5
Size: 47 4383 2081
Fragments arranged by size:
4383 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6511
StyI C'CwwG_G
Cuts at: 513 1065 2040 3129 3391 3950 513
Size: 552 975 1089 262 559 3074
Fragments arranged by size:
3074 1089 975 559 552 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 4538 4555 4708 4893 5338 325
Size: 1255 909 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 909 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2223 2466 2703
Size: 274 80 33 51 1443 246 243 237
Cuts at: 2703 2722 3291 3597 3741 4757 6469 96
Size: 19 569 306 144 1016 1712 138
Fragments arranged by size:
1712 1443 1016 569 306 274 246 243
237 144 138 80 51 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3503 3535 6471 6503 3503
Size: 32 2936 32 3511
Fragments arranged by size:
3511 2936 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6511
XbaI T'CTAG_A
Cuts at: 3847 3847
Size: 6511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3754 3754
Size: 6511
XhoI C'TCGA_G
Cuts at: 1943 2760 1943
Size: 817 5694
XmaI C'CCGG_G
Cuts at: 1870 3836 1870
Size: 1966 4545
XmnI GAAnn'nnTTC
Cuts at: 2540 4640 2540
Size: 2100 4411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3172 4319 276
Size: 53 83 186 936 1638 1147 2468
Fragments arranged by size:
2468 1638 1147 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI