Restriction map for
pFN21AB6161
(Circular) (Six-base) MAPSORT of: pFN21AB6161.seq Check: 5038 from: 1 to: 6502
pFN21AB6161 6502 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2068 2523 3845 1937
Size: 131 455 1322 4594
Fragments arranged by size:
4594 1322 455 131
Acc65I G'GTAC_C
Cuts at: 3823 3823
Size: 6502
AclI AA'CG_TT
Cuts at: 1563 3520 4629 5002 1563
Size: 1957 1109 373 3063
Fragments arranged by size:
3063 1957 1109 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4565 5949 6217 1632
Size: 2933 1384 268 1917
Fragments arranged by size:
2933 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 5401 6378 1203
Size: 4198 977 1327
Fragments arranged by size:
4198 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2694 1184
Size: 1510 4992
AlwNI CAG_nnn'CTG
Cuts at: 3119 4245 5817 3119
Size: 1126 1572 3804
Fragments arranged by size:
3804 1572 1126
ApaLI G'TGCA_C
Cuts at: 4559 5715 4559
Size: 1156 5346
ApoI r'AATT_y
Cuts at: 1493 2367 3080 3674 3811 4037 4189 6298 1493
Size: 874 713 594 137 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 713 594 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 3507 3827 1870
Size: 73 1564 320 4545
Fragments arranged by size:
4545 1564 320 73
BamHI G'GATC_C
Cuts at: 3832 4199 3832
Size: 367 6135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 3823 5278 618
Size: 325 217 627 420 1616 1455 1842
Fragments arranged by size:
1842 1616 1455 627 420 325 217
BanII G_rGCy'C
Cuts at: 1391 3821 1391
Size: 2430 4072
BbeI G_GCGC'C
Cuts at: 2211 2211
Size: 6502
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3193 928
Size: 971 1294 4237
Fragments arranged by size:
4237 1294 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2058 2092 4693 4727 1903
Size: 34 121 34 2601 34 3678
Fragments arranged by size:
3678 2601 121 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4394 5604 4394
Size: 1210 5292
BclI T'GATC_A
Cuts at: 1548 3370 1548
Size: 1822 4680
BfrBI ATG'CAT
Cuts at: 2118 2984 2118
Size: 866 5636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2205 5113 136
Size: 107 122 71 631 605 533 2908 1525
Fragments arranged by size:
2908 1525 631 605 533 122 107 71
BglII A'GATC_T
Cuts at: 2650 6497 2650
Size: 3847 2655
BlpI GC'TnA_GC
Cuts at: 3919 3919
Size: 6502
Bme1580I G_kGCm'C
Cuts at: 1790 4563 5719 1790
Size: 2773 1156 2573
Fragments arranged by size:
2773 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4233 5191 442
Size: 416 53 83 399 24 2816 958 1753
Fragments arranged by size:
2816 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3092 1056
Size: 2036 4466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3123 5162 1239
Size: 1884 2039 2579
Fragments arranged by size:
2579 2039 1884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2989 4627 5492 5790 2989
Size: 1638 865 298 3701
Fragments arranged by size:
3701 1638 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5165 882
Size: 617 3666 2219
Fragments arranged by size:
3666 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4293 493
Size: 711 3089 2702
Fragments arranged by size:
3089 2702 711
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 4309 4691 5326
Size: 53 83 186 936 675 2101 382 635
Cuts at: 5326 275
Size: 1451
Fragments arranged by size:
2101 1451 936 675 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4935 5333 5607 5754 6165 4935
Size: 398 274 147 411 5272
Fragments arranged by size:
5272 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2834 2864 3198 3228 1178
Size: 30 165 30 1431 30 334 30 4452
Fragments arranged by size:
4452 1431 334 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2676 3222 1186
Size: 1490 546 4466
Fragments arranged by size:
4466 1490 546
BseYI C'CCAG_C
Cuts at: 3136 5705 6201 3136
Size: 2569 496 3437
Fragments arranged by size:
3437 2569 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3401 1163
Size: 573 1665 4264
Fragments arranged by size:
4264 1665 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3952 4713 5741 1540
Size: 275 186 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4563 4648 5719 4563
Size: 85 1071 5346
Fragments arranged by size:
5346 1071 85
BsmI GAATG_Cn'
Cuts at: 2081 3180 4013 4106 2081
Size: 1099 833 93 4477
Fragments arranged by size:
4477 1099 833 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3821 4563 4648 5719 1391
Size: 399 2031 742 85 1071 2174
Fragments arranged by size:
2174 2031 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6165 6165
Size: 6502
BspHI T'CATG_A
Cuts at: 4391 6121 4391
Size: 1730 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3448 3857 844
Size: 2604 409 3489
Fragments arranged by size:
3489 2604 409
BsrBI CCG'CTC
Cuts at: 1394 1823 4389 1394
Size: 429 2566 3507
Fragments arranged by size:
3507 2566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4997 5171 65
Size: 1183 3749 174 1396
Fragments arranged by size:
3749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5146 5333 1700
Size: 110 3336 187 2869
Fragments arranged by size:
3336 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6502
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 4562 5574 4562
Size: 1012 5490
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6502
BstBI TT'CG_AA
Cuts at: 2101 5306 2101
Size: 3205 3297
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstYI r'GATC_y
Cuts at: 1972 2450 2558 2650 3709 3832 4199 4584 4601
Size: 478 108 92 1059 123 367 385 17
Cuts at: 4601 6053 6139 6151 6497 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1059 478 385 367 346 123
108 92 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2535 2535
Size: 6502
BtgI C'CryG_G
Cuts at: 513 1065 2004 2446 513
Size: 552 939 442 4569
Fragments arranged by size:
4569 939 552 442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5763
Fragments arranged by size:
5763 683 56
BtsI GCAGTG_nn'
Cuts at: 4018 4812 4832 4018
Size: 794 20 5688
Fragments arranged by size:
5688 794 20
ClaI AT'CG_AT
Cuts at: 1554 2260 1554
Size: 706 5796
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 2364 2763 2998 3806 2364
Size: 399 235 808 5060
Fragments arranged by size:
5060 808 399 235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3576 3949 4838
Size: 54 1425 50 275 18 1746 373 889
Cuts at: 4838 6467 8
Size: 1629 43
Fragments arranged by size:
1746 1629 1425 889 373 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3700 3767 4432 1353
Size: 2347 67 665 3423
Fragments arranged by size:
3423 2347 665 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3123 4565 5162 5949 6217 1239
Size: 393 1491 1442 597 787 268 1524
Fragments arranged by size:
1524 1491 1442 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2307 3201 2307
Size: 894 5608
EcoRI G'AATT_C
Cuts at: 3811 4189 3811
Size: 378 6124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3324 3565 3324
Size: 241 6261
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 2211 2889 5649 1678
Size: 533 678 2760 2531
Fragments arranged by size:
2760 2531 678 533
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2834 2866 3452 3484 3490
Size: 32 135 32 1262 32 586 32 6
Cuts at: 3490 3522 5143 5175 5217 5249 1373
Size: 32 1621 32 42 32 2626
Fragments arranged by size:
2626 1621 1262 586 135 42 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2019 2038 2069 2355 3846 4092
Size: 988 281 81 19 31 286 1491 246
Cuts at: 4092 669
Size: 3079
Fragments arranged by size:
3079 1491 988 286 281 246 81 31
19
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6502
HpaI GTT'AAC
Cuts at: 2019 4092 2019
Size: 2073 4429
KasI G'GCGC_C
Cuts at: 2207 2207
Size: 6502
KpnI G_GTAC'C
Cuts at: 3827 3827
Size: 6502
MfeI C'AATT_G
Cuts at: 2217 2598 3093 4101 2217
Size: 381 495 1008 4618
Fragments arranged by size:
4618 1008 495 381
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2311 2877 2981 5616 5800 1305
Size: 1006 566 104 2635 184 2007
Fragments arranged by size:
2635 2007 1006 566 184 104
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3578 10
Size: 54 1425 343 1746 2934
Fragments arranged by size:
2934 1746 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2816 4460 4819 4978 518
Size: 648 1650 1644 359 159 2042
Fragments arranged by size:
2042 1650 1644 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3918 4595 5743 5988 1606
Size: 2312 677 1148 245 2120
Fragments arranged by size:
2312 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2208 2208
Size: 6502
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5011
Fragments arranged by size:
5011 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NheI G'CTAG_C
Cuts at: 1052 3088 1052
Size: 2036 4466
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NsiI A_TGCA'T
Cuts at: 2120 2986 2120
Size: 866 5636
NspI r_CATG'y
Cuts at: 1132 1229 3860 5405 1132
Size: 97 2631 1545 2229
Fragments arranged by size:
2631 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6502
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2396 1108
Size: 1288 5214
PfoI T'CCnGG_A
Cuts at: 3055 3055
Size: 6502
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 6084 6116 4571
Size: 32 1481 32 4957
Fragments arranged by size:
4957 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 3237 4072 3237
Size: 835 5667
PspXI vC'TCGA_Gb
Cuts at: 3507 3507
Size: 6502
PstI C_TGCA'G
Cuts at: 830 3854 830
Size: 3024 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
SalI G'TCGA_C
Cuts at: 1936 2067 3844 1936
Size: 131 1777 4594
Fragments arranged by size:
4594 1777 131
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SfcI C'TryA_G
Cuts at: 826 1045 2094 3404 3721 3850 4985 5666 5857
Size: 219 1049 1310 317 129 1135 681 191
Cuts at: 5857 826
Size: 1471
Fragments arranged by size:
1471 1310 1135 1049 681 317 219 191
129
SfoI GGC'GCC
Cuts at: 2209 2209
Size: 6502
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 3829 1872
Size: 1957 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2968 3507 4606 5507 5769 820
Size: 197 926 1025 539 1099 901 262 1553
Fragments arranged by size:
1553 1099 1025 926 901 539 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6502
StyI C'CwwG_G
Cuts at: 513 1065 2004 2395 3941 513
Size: 552 939 391 1546 3074
Fragments arranged by size:
3074 1546 939 552 391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2586 3333 3347 4529 4546 4699 4884
Size: 1255 1006 747 14 1182 17 153 185
Cuts at: 4884 5329 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1182 1006 747 445 185 153
17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4748 6460 96
Size: 274 80 33 51 1443 2771 1712 138
Fragments arranged by size:
2771 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 2482 3838 2482
Size: 1356 5146
XhoI C'TCGA_G
Cuts at: 1943 3507 1943
Size: 1564 4938
XmaI C'CCGG_G
Cuts at: 1870 3827 1870
Size: 1957 4545
XmnI GAAnn'nnTTC
Cuts at: 4631 4631
Size: 6502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI
PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI