Restriction map for
pFN21AB6156
(Circular) (Six-base) MAPSORT of: pFN21AB6156.seq Check: 2341 from: 1 to: 6616
pFN21AB6156 6616 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4426 278
Size: 53 83 186 936 2890 2468
Fragments arranged by size:
2890 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2011 3959 1937
Size: 74 1948 4594
Fragments arranged by size:
4594 1948 74
Acc65I G'GTAC_C
Cuts at: 2034 3937 2034
Size: 1903 4713
AclI AA'CG_TT
Cuts at: 1563 4743 5116 1563
Size: 3180 373 3063
Fragments arranged by size:
3180 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2474 2759 2792 2891 4679 6063 6331
Size: 406 436 285 33 99 1788 1384 268
Cuts at: 6331 1632
Size: 1917
Fragments arranged by size:
1917 1788 1384 436 406 285 268 99
33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6419
AflIII A'CryG_T
Cuts at: 1203 5515 6492 1203
Size: 4312 977 1327
Fragments arranged by size:
4312 1327 977
AgeI A'CCGG_T
Cuts at: 5447 5447
Size: 6616
AhdI GACnn_n'nnGTC
Cuts at: 5345 5345
Size: 6616
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3168 1184
Size: 1984 4632
AlwNI CAG_nnn'CTG
Cuts at: 2626 3406 4359 5931 2626
Size: 780 953 1572 3311
Fragments arranged by size:
3311 1572 953 780
ApaLI G'TGCA_C
Cuts at: 4673 5829 4673
Size: 1156 5460
ApoI r'AATT_y
Cuts at: 1493 2852 3181 3195 3925 4151 4303 6412 1493
Size: 1359 329 14 730 226 152 2109 1697
Fragments arranged by size:
2109 1697 1359 730 329 226 152 14
AseI AT'TA_AT
Cuts at: 160 1032 5170 160
Size: 872 4138 1606
Fragments arranged by size:
4138 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6616
AvaI C'yCGr_G
Cuts at: 1870 1943 2538 3941 1870
Size: 73 595 1403 4545
Fragments arranged by size:
4545 1403 595 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2026 2059 2026
Size: 33 6583
BamHI G'GATC_C
Cuts at: 3833 3946 4313 3833
Size: 113 367 6136
Fragments arranged by size:
6136 367 113
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2034 3937 5392 618
Size: 325 217 627 247 1903 1455 1842
Fragments arranged by size:
1903 1842 1455 627 325 247 217
BanII G_rGCy'C
Cuts at: 1391 2691 3794 3809 3935 1391
Size: 1300 1103 15 126 4072
Fragments arranged by size:
4072 1300 1103 126 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3378 928
Size: 971 1479 4166
Fragments arranged by size:
4166 1479 971
BbvCI CC'TCA_GC
Cuts at: 3060 3105 3060
Size: 45 6571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4807 4841 1903
Size: 34 2870 34 3678
Fragments arranged by size:
3678 2870 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4508 5718 4508
Size: 1210 5406
BclI T'GATC_A
Cuts at: 1548 2746 1548
Size: 1198 5418
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5227 136
Size: 107 122 71 631 605 3555 1525
Fragments arranged by size:
3555 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3230 3258 6611 3230
Size: 28 3353 3235
Fragments arranged by size:
3353 3235 28
BlpI GC'TnA_GC
Cuts at: 4033 4033
Size: 6616
Bme1580I G_kGCm'C
Cuts at: 1790 3499 4677 5833 1790
Size: 1709 1178 1156 2573
Fragments arranged by size:
2573 1709 1178 1156
BmgBI CAC'GTC
Cuts at: 1318 2261 1318
Size: 943 5673
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3067 4347 5305
Size: 416 53 83 399 24 1650 1280 958
Cuts at: 5305 442
Size: 1753
Fragments arranged by size:
1753 1650 1280 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 3365 3455 3482 3507 3606 3648 3708
Size: 1388 738 90 27 25 99 42 60
Cuts at: 3708 3777 3831 5276 1239
Size: 69 54 1445 2579
Fragments arranged by size:
2579 1445 1388 738 99 90 69 60
54 42 27 25
Bpu10I CC'TnA_GC
Cuts at: 3060 3105 3060
Size: 45 6571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3299 4741 5606 5904 3299
Size: 1442 865 298 4011
Fragments arranged by size:
4011 1442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5279 882
Size: 617 3780 2219
Fragments arranged by size:
3780 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4407 493
Size: 711 3203 2702
Fragments arranged by size:
3203 2702 711
BsaBI GATnn'nnATC
Cuts at: 6610 6610
Size: 6616
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4423 4805 5440 275
Size: 53 83 186 936 2890 382 635 1451
Fragments arranged by size:
2890 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2802 3605 3665 3722 3902 5049 5447 5721 5868
Size: 803 60 57 180 1147 398 274 147
Cuts at: 5868 6279 2802
Size: 411 3139
Fragments arranged by size:
3139 1147 803 411 398 274 180 147
60 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6391
Fragments arranged by size:
6391 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2139 2265 3573 1186
Size: 953 126 1308 4229
Fragments arranged by size:
4229 1308 953 126
BseYI C'CCAG_C
Cuts at: 2069 5819 6315 2069
Size: 3750 496 2370
Fragments arranged by size:
3750 2370 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6043
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3649 3787 4066 4827 5855 1540
Size: 275 186 1648 138 279 761 1028 2301
Fragments arranged by size:
2301 1648 1028 761 279 275 186 138
BsiHKAI G_wGCw'C
Cuts at: 3524 3809 4677 4762 5833 3524
Size: 285 868 85 1071 4307
Fragments arranged by size:
4307 1071 868 285 85
BsmI GAATG_Cn'
Cuts at: 3313 4127 4220 3313
Size: 814 93 5709
Fragments arranged by size:
5709 814 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2691 3499 3524 3794 3809 3935 4677
Size: 399 901 808 25 270 15 126 742
Cuts at: 4677 4762 5833 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 901 808 742 399 270 126
85 25 15
BspEI T'CCGG_A
Cuts at: 3605 3665 3722 3902 6279 3605
Size: 60 57 180 2377 3942
Fragments arranged by size:
3942 2377 180 60 57
BspHI T'CATG_A
Cuts at: 4505 6235 4505
Size: 1730 4886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 3971 844
Size: 1800 1327 3489
Fragments arranged by size:
3489 1800 1327
BsrBI CCG'CTC
Cuts at: 1394 1823 4503 1394
Size: 429 2680 3507
Fragments arranged by size:
3507 2680 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3054 5111 5285 65
Size: 1183 1806 2057 174 1396
Fragments arranged by size:
2057 1806 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5260 5447 1700
Size: 110 3450 187 2869
Fragments arranged by size:
3450 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6616
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6616
BssSI C'ACGA_G
Cuts at: 4676 5688 4676
Size: 1012 5604
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6616
BstBI TT'CG_AA
Cuts at: 5420 5420
Size: 6616
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6616
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2242
Size: 6616
BstYI r'GATC_y
Cuts at: 1972 2520 3230 3258 3430 3833 3946 4313 4698
Size: 548 710 28 172 403 113 367 385
Cuts at: 4698 4715 6167 6253 6265 6611 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 710 548 403 385 367 346
172 113 86 28 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3649 3706 513
Size: 552 2584 57 3423
Fragments arranged by size:
3423 2584 552 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5877
Fragments arranged by size:
5877 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 2380 3150 3739 4132 4926 4946 2125
Size: 255 770 589 393 794 20 3795
Fragments arranged by size:
3795 794 770 589 393 255 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6616
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6581
DraI TTT'AAA
Cuts at: 3920 3920
Size: 6616
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5623 809
Size: 507 4307 1802
Fragments arranged by size:
4307 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3646 3703 3784
Size: 54 1425 50 275 18 1816 57 81
Cuts at: 3784 4063 4952 6581 8
Size: 279 889 1629 43
Fragments arranged by size:
1816 1629 1425 889 279 275 81 57
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 3784 4063 1537
Size: 275 1834 138 279 4090
Fragments arranged by size:
4090 1834 279 275 138
EarI CTCTTCn'nnn_
Cuts at: 1353 2735 3089 3132 4546 1353
Size: 1382 354 43 1414 3423
Fragments arranged by size:
3423 1414 1382 354 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5203 5577 5723 387
Size: 1064 138 3614 374 146 1280
Fragments arranged by size:
3614 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3807 3807
Size: 6616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2474 2627 2759 2792 2891 3365
Size: 393 406 436 153 132 33 99 474
Cuts at: 3365 3455 3482 3507 3606 3648 3708 3777 3831
Size: 90 27 25 99 42 60 69 54
Cuts at: 3831 4679 5276 6063 6331 1239
Size: 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 597 474 436 406 393
268 153 132 99 99 90 69 60
54 42 33 27 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3359 3461 3359
Size: 102 6514
EcoO109I rG'GnC_Cy
Cuts at: 3453 3453
Size: 6616
EcoRI G'AATT_C
Cuts at: 3925 4303 3925
Size: 378 6238
FspI TGC'GCA
Cuts at: 5122 5122
Size: 6616
HaeII r_GCGC'y
Cuts at: 1678 5763 1678
Size: 4085 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2971 3003 3098 3130 3222
Size: 32 135 32 1399 32 95 32 92
Cuts at: 3222 3254 5257 5289 5331 5363 1373
Size: 32 2003 32 42 32 2626
Fragments arranged by size:
2626 2003 1399 135 95 92 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2012 2209 3960 4206 669
Size: 988 281 74 197 1751 246 3079
Fragments arranged by size:
3079 1751 988 281 246 197 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6616
HpaI GTT'AAC
Cuts at: 2209 4206 2209
Size: 1997 4619
KpnI G_GTAC'C
Cuts at: 2038 3941 2038
Size: 1903 4713
MfeI C'AATT_G
Cuts at: 4215 4215
Size: 6616
MluI A'CGCG_T
Cuts at: 6492 6492
Size: 6616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2177 2739 3288 5730 5914 1305
Size: 872 562 549 2442 184 2007
Fragments arranged by size:
2442 2007 872 562 549 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4794
Fragments arranged by size:
4794 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 4574 4933 5092 518
Size: 648 1074 2334 359 159 2042
Fragments arranged by size:
2334 2042 1074 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4032 4709 5857 6102 1606
Size: 2426 677 1148 245 2120
Fragments arranged by size:
2426 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3706 513
Size: 552 2641 3423
Fragments arranged by size:
3423 2641 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6616
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6616
NotI GC'GGCC_GC
Cuts at: 4063 4063
Size: 6616
NspI r_CATG'y
Cuts at: 1132 1229 2782 3974 5519 1132
Size: 97 1553 1192 1545 2229
Fragments arranged by size:
2229 1553 1545 1192 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6616
PciI A'CATG_T
Cuts at: 5515 5515
Size: 6616
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6616
PfoI T'CCnGG_A
Cuts at: 2604 3459 2604
Size: 855 5761
PmeI GTTT'AAAC
Cuts at: 3920 3920
Size: 6616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4685 4717 6198 6230 4685
Size: 32 1481 32 5071
Fragments arranged by size:
5071 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3453 3453
Size: 6616
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6616
PsiI TTA'TAA
Cuts at: 4186 4186
Size: 6616
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3607 3639 3607
Size: 32 6584
PstI C_TGCA'G
Cuts at: 830 2652 3147 3360 3968 830
Size: 1822 495 213 608 3478
Fragments arranged by size:
3478 1822 608 495 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6616
SacI G_AGCT'C
Cuts at: 3809 3809
Size: 6616
SalI G'TCGA_C
Cuts at: 1936 2010 3958 1936
Size: 74 1948 4594
Fragments arranged by size:
4594 1948 74
SapI GCTCTTCn'nnn_
Cuts at: 3089 3132 3089
Size: 43 6573
SbfI CC_TGCA'GG
Cuts at: 3968 3968
Size: 6616
ScaI AGT'ACT
Cuts at: 4864 6576 4864
Size: 1712 4904
SexAI A'CCwGG_T
Cuts at: 2619 2619
Size: 6616
SfcI C'TryA_G
Cuts at: 826 1045 2648 3143 3356 3448 3742 3964 5099
Size: 219 1603 495 213 92 294 222 1135
Cuts at: 5099 5780 5971 826
Size: 681 191 1471
Fragments arranged by size:
1603 1471 1135 681 495 294 222 219
213 191 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6616
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6616
SmaI CCC'GGG
Cuts at: 1872 3943 1872
Size: 2071 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2538 3314 4720 5621 5883 820
Size: 197 926 595 776 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 776 595 262 197
SnaBI TAC'GTA
Cuts at: 493 4407 493
Size: 3914 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6616
SphI G_CATG'C
Cuts at: 1132 3974 1132
Size: 2842 3774
SspI AAT'ATT
Cuts at: 5 52 4540 5
Size: 47 4488 2081
Fragments arranged by size:
4488 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6616
StyI C'CwwG_G
Cuts at: 513 1065 2212 2895 3366 3706 4055 513
Size: 552 1147 683 471 340 349 3074
Fragments arranged by size:
3074 1147 683 552 471 349 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4643 4660 4813 4998 5443 325
Size: 1255 3063 17 153 185 445 1498
Fragments arranged by size:
3063 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4862 6574 96
Size: 274 80 33 51 1443 2885 1712 138
Fragments arranged by size:
2885 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6576 6608 6576
Size: 32 6584
Tth111I GACn'n_nGTC
Cuts at: 1316 3245 1316
Size: 1929 4687
XbaI T'CTAG_A
Cuts at: 3952 3952
Size: 6616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3373 3424 3373
Size: 51 6565
XhoI C'TCGA_G
Cuts at: 1943 2538 1943
Size: 595 6021
XmaI C'CCGG_G
Cuts at: 1870 3941 1870
Size: 2071 4545
XmnI GAAnn'nnTTC
Cuts at: 4745 4745
Size: 6616
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4424 276
Size: 53 83 186 936 2890 2468
Fragments arranged by size:
2890 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PvuII RsrII SacII SanDI
SfiI SfoI SrfI SwaI