Restriction map for
pFN21AB6154
(Circular) (Six-base) MAPSORT of: pFN21AB6154.seq Check: 4834 from: 1 to: 6631
pFN21AB6154 6631 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3633 3633
Size: 6631
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4441 278
Size: 53 83 186 936 2905 2468
Fragments arranged by size:
2905 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3974 1937
Size: 2037 4594
Acc65I G'GTAC_C
Cuts at: 3952 3952
Size: 6631
AclI AA'CG_TT
Cuts at: 1563 4758 5131 1563
Size: 3195 373 3063
Fragments arranged by size:
3195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2402 2670 2786 2921 3429 3741 3945 4694
Size: 770 268 116 135 508 312 204 749
Cuts at: 4694 6078 6346 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 770 749 508 312 268 268
204 135 116
AflII C'TTAA_G
Cuts at: 820 1017 3386 820
Size: 197 2369 4065
Fragments arranged by size:
4065 2369 197
AflIII A'CryG_T
Cuts at: 1203 3778 5530 6507 1203
Size: 2575 1752 977 1327
Fragments arranged by size:
2575 1752 1327 977
AgeI A'CCGG_T
Cuts at: 5462 5462
Size: 6631
AhdI GACnn_n'nnGTC
Cuts at: 5360 5360
Size: 6631
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6631
AlwNI CAG_nnn'CTG
Cuts at: 4374 5946 4374
Size: 1572 5059
ApaLI G'TGCA_C
Cuts at: 4688 5844 4688
Size: 1156 5475
ApoI r'AATT_y
Cuts at: 1493 3295 3940 4166 4318 6427 1493
Size: 1802 645 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 645 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3144 3420 5185 160
Size: 872 2112 276 1765 1606
Fragments arranged by size:
2112 1765 1606 872 276
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 3332 3956 1870
Size: 73 308 1081 624 4545
Fragments arranged by size:
4545 1081 624 308 73
BamHI G'GATC_C
Cuts at: 2198 3961 4328 2198
Size: 1763 367 4501
Fragments arranged by size:
4501 1763 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2720 3341 3952 5407 618
Size: 325 217 627 933 621 611 1455 1842
Fragments arranged by size:
1842 1455 933 627 621 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2527 3370 3654 3950 1391
Size: 1136 843 284 296 4072
Fragments arranged by size:
4072 1136 843 296 284
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3912 928
Size: 971 2013 3647
Fragments arranged by size:
3647 2013 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2273 2307 3323 3357 4822 4856 1903
Size: 34 336 34 1016 34 1465 34 3678
Fragments arranged by size:
3678 1465 1016 336 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2493 3448 4523 5733 2493
Size: 955 1075 1210 3391
Fragments arranged by size:
3391 1210 1075 955
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6631
BfrBI ATG'CAT
Cuts at: 2665 3846 2665
Size: 1181 5450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5242 136
Size: 107 122 71 631 605 3570 1525
Fragments arranged by size:
3570 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2385 3856 6626 2385
Size: 1471 2770 2390
Fragments arranged by size:
2770 2390 1471
BlpI GC'TnA_GC
Cuts at: 4048 4048
Size: 6631
Bme1580I G_kGCm'C
Cuts at: 1790 2417 4692 5848 1790
Size: 627 2275 1156 2573
Fragments arranged by size:
2573 2275 1156 627
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6631
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4362 5320 442
Size: 416 53 83 399 24 2945 958 1753
Fragments arranged by size:
2945 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5291 1239
Size: 4052 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3354 3620 3824 4756 5621 5919 3354
Size: 266 204 932 865 298 4066
Fragments arranged by size:
4066 932 865 298 266 204
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2248 2999 5294 882
Size: 617 749 751 2295 2219
Fragments arranged by size:
2295 2219 751 749 617
BsaAI yAC'GTr
Cuts at: 493 1204 4422 493
Size: 711 3218 2702
Fragments arranged by size:
3218 2702 711
BsaBI GATnn'nnATC
Cuts at: 6625 6625
Size: 6631
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4438 4820 5455 275
Size: 53 83 186 936 2905 382 635 1451
Fragments arranged by size:
2905 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5064 5462 5736 5883 6294 5064
Size: 398 274 147 411 5401
Fragments arranged by size:
5401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6406
Fragments arranged by size:
6406 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2517 3209 3821 1186
Size: 1331 692 612 3996
Fragments arranged by size:
3996 1331 692 612
BseYI C'CCAG_C
Cuts at: 5834 6330 5834
Size: 496 6135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6058
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4081 4842 5870 1540
Size: 275 186 2080 761 1028 2301
Fragments arranged by size:
2301 2080 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4692 4777 5848 4692
Size: 85 1071 5475
Fragments arranged by size:
5475 1071 85
BsmI GAATG_Cn'
Cuts at: 2273 2749 3478 3848 4142 4235 2273
Size: 476 729 370 294 93 4669
Fragments arranged by size:
4669 729 476 370 294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2417 2527 3370 3654 3950 4692 4777
Size: 399 627 110 843 284 296 742 85
Cuts at: 4777 5848 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 843 742 627 399 296 284
110 85
BspEI T'CCGG_A
Cuts at: 6294 6294
Size: 6631
BspHI T'CATG_A
Cuts at: 3115 4520 6250 3115
Size: 1405 1730 3496
Fragments arranged by size:
3496 1730 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3633 3986 844
Size: 2789 353 3489
Fragments arranged by size:
3489 2789 353
BsrBI CCG'CTC
Cuts at: 1394 1823 4518 1394
Size: 429 2695 3507
Fragments arranged by size:
3507 2695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3483 5126 5300 65
Size: 1183 2235 1643 174 1396
Fragments arranged by size:
2235 1643 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5275 5462 1700
Size: 110 3465 187 2869
Fragments arranged by size:
3465 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2511 96
Size: 2415 4216
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6631
BssSI C'ACGA_G
Cuts at: 4691 5703 4691
Size: 1012 5619
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6631
BstBI TT'CG_AA
Cuts at: 5435 5435
Size: 6631
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6631
BstXI CCAn_nnnn'nTGG
Cuts at: 3163 3163
Size: 6631
BstYI r'GATC_y
Cuts at: 1972 2063 2198 2385 3856 3961 4328 4713 4730
Size: 91 135 187 1471 105 367 385 17
Cuts at: 4730 6182 6268 6280 6626 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1471 1452 385 367 346 187 135
105 91 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6079
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5892
Fragments arranged by size:
5892 683 56
BtsI GCAGTG_nn'
Cuts at: 2291 4147 4941 4961 2291
Size: 1856 794 20 3961
Fragments arranged by size:
3961 1856 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6596
DraI TTT'AAA
Cuts at: 2470 3935 2470
Size: 1465 5166
DraIII CAC_nnn'GTG
Cuts at: 2548 2548
Size: 6631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5638 809
Size: 507 4322 1802
Fragments arranged by size:
4322 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 4078 4967
Size: 54 1425 50 275 18 208 2040 889
Cuts at: 4967 6596 8
Size: 1629 43
Fragments arranged by size:
2040 1629 1425 889 275 208 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4078 1537
Size: 275 2266 4090
Fragments arranged by size:
4090 2266 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2445 3567 4561 1353
Size: 1092 1122 994 3423
Fragments arranged by size:
3423 1122 1092 994
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5218 5592 5738 387
Size: 1064 138 3629 374 146 1280
Fragments arranged by size:
3629 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 2670 2786 2921 3429 3741 3945
Size: 393 770 268 116 135 508 312 204
Cuts at: 3945 4694 5291 6078 6346 1239
Size: 749 597 787 268 1524
Fragments arranged by size:
1524 787 770 749 597 508 393 312
268 268 204 135 116
EcoO109I rG'GnC_Cy
Cuts at: 2248 3160 2248
Size: 912 5719
EcoRI G'AATT_C
Cuts at: 3295 3940 4318 3295
Size: 645 378 5608
Fragments arranged by size:
5608 645 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2628 2926 2628
Size: 298 6333
FspI TGC'GCA
Cuts at: 5137 5137
Size: 6631
HaeII r_GCGC'y
Cuts at: 1678 5778 1678
Size: 4100 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2741 2773 3587
Size: 32 135 32 476 32 661 32 814
Cuts at: 3587 3619 5272 5304 5346 5378 1373
Size: 32 1653 32 42 32 2626
Fragments arranged by size:
2626 1653 814 661 476 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2213 3643 3975 4221 669
Size: 988 281 275 1430 332 246 3079
Fragments arranged by size:
3079 1430 988 332 281 275 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6631
HpaI GTT'AAC
Cuts at: 3643 4221 3643
Size: 578 6053
KpnI G_GTAC'C
Cuts at: 3956 3956
Size: 6631
MfeI C'AATT_G
Cuts at: 3467 4230 3467
Size: 763 5868
MluI A'CGCG_T
Cuts at: 6507 6507
Size: 6631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5745 5929 1305
Size: 4440 184 2007
Fragments arranged by size:
4440 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4809
Fragments arranged by size:
4809 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4589 4948 5107 518
Size: 648 3423 359 159 2042
Fragments arranged by size:
3423 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 3688 4047 4724 5872 6117 1606
Size: 422 1660 359 677 1148 245 2120
Fragments arranged by size:
2120 1660 1148 677 422 359 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6079
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6631
NotI GC'GGCC_GC
Cuts at: 4078 4078
Size: 6631
NsiI A_TGCA'T
Cuts at: 2667 3848 2667
Size: 1181 5450
NspI r_CATG'y
Cuts at: 1132 1229 3782 3850 3989 5534 1132
Size: 97 2553 68 139 1545 2229
Fragments arranged by size:
2553 2229 1545 139 97 68
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6631
PciI A'CATG_T
Cuts at: 3778 5530 3778
Size: 1752 4879
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2123 1108
Size: 1015 5616
PfoI T'CCnGG_A
Cuts at: 2201 2201
Size: 6631
PmeI GTTT'AAAC
Cuts at: 3935 3935
Size: 6631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4700 4732 6213 6245 4700
Size: 32 1481 32 5086
Fragments arranged by size:
5086 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 3160 2248
Size: 912 5719
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6631
PsiI TTA'TAA
Cuts at: 4201 4201
Size: 6631
PstI C_TGCA'G
Cuts at: 830 2757 3983 830
Size: 1927 1226 3478
Fragments arranged by size:
3478 1927 1226
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6631
SalI G'TCGA_C
Cuts at: 1936 3973 1936
Size: 2037 4594
SbfI CC_TGCA'GG
Cuts at: 3983 3983
Size: 6631
ScaI AGT'ACT
Cuts at: 3086 3637 4879 6591 3086
Size: 551 1242 1712 3126
Fragments arranged by size:
3126 1712 1242 551
SfcI C'TryA_G
Cuts at: 826 1045 2753 3059 3789 3979 5114 5795 5986
Size: 219 1708 306 730 190 1135 681 191
Cuts at: 5986 826
Size: 1471
Fragments arranged by size:
1708 1471 1135 730 681 306 219 191
190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6631
SmaI CCC'GGG
Cuts at: 1872 3958 1872
Size: 2086 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3369 3386 3599 3803 4735 5636
Size: 197 926 1426 17 213 204 932 901
Cuts at: 5636 5898 820
Size: 262 1553
Fragments arranged by size:
1553 1426 932 926 901 262 213 204
197 17
SnaBI TAC'GTA
Cuts at: 493 4422 493
Size: 3929 2702
SpeI A'CTAG_T
Cuts at: 152 2186 3700 152
Size: 2034 1514 3083
Fragments arranged by size:
3083 2034 1514
SphI G_CATG'C
Cuts at: 1132 3989 1132
Size: 2857 3774
SspI AAT'ATT
Cuts at: 5 52 3871 4555 5
Size: 47 3819 684 2081
Fragments arranged by size:
3819 2081 684 47
StuI AGG'CCT
Cuts at: 1517 2088 1517
Size: 571 6060
StyI C'CwwG_G
Cuts at: 513 1065 2476 2633 3156 3606 4070 513
Size: 552 1411 157 523 450 464 3074
Fragments arranged by size:
3074 1411 552 523 464 450 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4658 4675 4828 5013 5458 325
Size: 1255 3078 17 153 185 445 1498
Fragments arranged by size:
3078 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 3084 3635
Size: 274 80 33 51 1443 534 573 551
Cuts at: 3635 4877 6589 96
Size: 1242 1712 138
Fragments arranged by size:
1712 1443 1242 573 551 534 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6591 6623 6591
Size: 32 6599
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6631
XbaI T'CTAG_A
Cuts at: 2881 3967 2881
Size: 1086 5545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2515 2987 2515
Size: 472 6159
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6631
XmaI C'CCGG_G
Cuts at: 1870 3956 1870
Size: 2086 4545
XmnI GAAnn'nnTTC
Cuts at: 2387 2454 3522 4760 2387
Size: 67 1068 1238 4258
Fragments arranged by size:
4258 1238 1068 67
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4439 276
Size: 53 83 186 936 2905 2468
Fragments arranged by size:
2905 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI