Restriction map for
pFN21AB6153
(Circular) (Six-base) MAPSORT of: pFN21AB6153.seq Check: 123 from: 1 to: 6496
pFN21AB6153 6496 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3226 3226
Size: 6496
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4306 278
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3688 3839 1937
Size: 1751 151 4594
Fragments arranged by size:
4594 1751 151
Acc65I G'GTAC_C
Cuts at: 3817 3817
Size: 6496
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2460 2664 2777 3116 3248 3452 4559 5943
Size: 828 204 113 339 132 204 1107 1384
Cuts at: 5943 6211 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1107 828 339 268 204 204
132 113
AflII C'TTAA_G
Cuts at: 820 1017 2654 820
Size: 197 1637 4662
Fragments arranged by size:
4662 1637 197
AflIII A'CryG_T
Cuts at: 1203 2721 5395 6372 1203
Size: 1518 2674 977 1327
Fragments arranged by size:
2674 1518 1327 977
AgeI A'CCGG_T
Cuts at: 5327 5327
Size: 6496
AhdI GACnn_n'nnGTC
Cuts at: 5225 5225
Size: 6496
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6496
AlwNI CAG_nnn'CTG
Cuts at: 3185 3241 3638 4239 5811 3185
Size: 56 397 601 1572 3870
Fragments arranged by size:
3870 1572 601 397 56
ApaLI G'TGCA_C
Cuts at: 3293 4553 5709 3293
Size: 1260 1156 4080
Fragments arranged by size:
4080 1260 1156
ApoI r'AATT_y
Cuts at: 1493 2260 2600 2686 2714 3805 4031 4183 6292
Size: 767 340 86 28 1091 226 152 2109
Cuts at: 6292 1493
Size: 1697
Fragments arranged by size:
2109 1697 1091 767 340 226 152 86
28
AseI AT'TA_AT
Cuts at: 160 1032 2190 5050 160
Size: 872 1158 2860 1606
Fragments arranged by size:
2860 1606 1158 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 3821 1870
Size: 73 1878 4545
Fragments arranged by size:
4545 1878 73
BamHI G'GATC_C
Cuts at: 3626 3826 4193 3626
Size: 200 367 5929
Fragments arranged by size:
5929 367 200
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3353 3413 3817 5272 618
Size: 325 217 627 1566 60 404 1455 1842
Fragments arranged by size:
1842 1566 1455 627 404 325 217 60
BanII G_rGCy'C
Cuts at: 1391 3260 3815 1391
Size: 1869 555 4072
Fragments arranged by size:
4072 1869 555
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2166 2466 2963 3375 928
Size: 971 267 300 497 412 4049
Fragments arranged by size:
4049 971 497 412 300 267
BbvCI CC'TCA_GC
Cuts at: 2302 2302
Size: 6496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4687 4721 1903
Size: 34 2750 34 3678
Fragments arranged by size:
3678 2750 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4388 5598 4388
Size: 1210 5286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BfrBI ATG'CAT
Cuts at: 2569 3778 2569
Size: 1209 5287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2405 5107 136
Size: 107 122 71 631 605 733 2702 1525
Fragments arranged by size:
2702 1525 733 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2764 3708 6491 2764
Size: 944 2783 2769
Fragments arranged by size:
2783 2769 944
BlpI GC'TnA_GC
Cuts at: 3913 3913
Size: 6496
Bme1580I G_kGCm'C
Cuts at: 1790 2531 3297 4557 5713 1790
Size: 741 766 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 766 741
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4227 5185 442
Size: 416 53 83 399 24 2810 958 1753
Fragments arranged by size:
2810 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3183 3183
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 5156 1239
Size: 842 3075 2579
Fragments arranged by size:
3075 2579 842
Bpu10I CC'TnA_GC
Cuts at: 2302 2302
Size: 6496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4621 5486 5784 4621
Size: 865 298 5333
Fragments arranged by size:
5333 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5159 882
Size: 617 3660 2219
Fragments arranged by size:
3660 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2722 4287 493
Size: 711 1518 1565 2702
Fragments arranged by size:
2702 1565 1518 711
BsaBI GATnn'nnATC
Cuts at: 3639 6490 3639
Size: 2851 3645
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4303 4685 5320 275
Size: 53 83 186 936 2770 382 635 1451
Fragments arranged by size:
2770 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4929 5327 5601 5748 6159 4929
Size: 398 274 147 411 5266
Fragments arranged by size:
5266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2912 2942 3337 3367 1178
Size: 30 165 30 1509 30 395 30 4307
Fragments arranged by size:
4307 1509 395 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2936 1186
Size: 1750 4746
BseYI C'CCAG_C
Cuts at: 5699 6195 5699
Size: 496 6000
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2730 1163
Size: 573 994 4929
Fragments arranged by size:
4929 994 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3946 4707 5735 1540
Size: 275 186 913 1032 761 1028 2301
Fragments arranged by size:
2301 1032 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3297 4557 4642 5713 3297
Size: 1260 85 1071 4080
Fragments arranged by size:
4080 1260 1071 85
BsmI GAATG_Cn'
Cuts at: 3116 4007 4100 3116
Size: 891 93 5512
Fragments arranged by size:
5512 891 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2531 3260 3297 3815 4557 4642 5713
Size: 399 741 729 37 518 742 85 1071
Cuts at: 5713 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 741 729 518 399 85
37
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 4385 6115 4385
Size: 1730 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3226 3851 844
Size: 2382 625 3489
Fragments arranged by size:
3489 2382 625
BsrBI CCG'CTC
Cuts at: 1394 1823 4383 1394
Size: 429 2560 3507
Fragments arranged by size:
3507 2560 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4991 5165 65
Size: 1183 3743 174 1396
Fragments arranged by size:
3743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5140 5327 1700
Size: 110 3330 187 2869
Fragments arranged by size:
3330 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2269 96
Size: 2173 4323
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 4556 5568 4556
Size: 1012 5484
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6496
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstXI CCAn_nnnn'nTGG
Cuts at: 2433 2902 3683 2433
Size: 469 781 5246
Fragments arranged by size:
5246 781 469
BstYI r'GATC_y
Cuts at: 1972 2764 3626 3708 3826 4193 4578 4595 6047
Size: 792 862 82 118 367 385 17 1452
Cuts at: 6047 6133 6145 6491 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 862 792 385 367 346 118
86 82 17 12
Bsu36I CC'TnA_GG
Cuts at: 3158 3158
Size: 6496
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5005
Fragments arranged by size:
5005 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3737 487
Size: 683 56 2511 3246
Fragments arranged by size:
3246 2511 683 56
BtsI GCAGTG_nn'
Cuts at: 2507 2726 2907 2958 4012 4806 4826 2507
Size: 219 181 51 1054 794 20 4177
Fragments arranged by size:
4177 1054 794 219 181 51 20
ClaI AT'CG_AT
Cuts at: 1554 2467 1554
Size: 913 5583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6461
DraI TTT'AAA
Cuts at: 3496 3800 3496
Size: 304 6192
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2079 5503 809
Size: 507 763 3424 1802
Fragments arranged by size:
3424 1802 763 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2911 3787 3943
Size: 54 1425 50 275 18 1081 876 156
Cuts at: 3943 4832 6461 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1081 889 876 275 156 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 3943 1537
Size: 275 1099 1032 4090
Fragments arranged by size:
4090 1099 1032 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2865 2940 3044 4426 1353
Size: 1512 75 104 1382 3423
Fragments arranged by size:
3423 1512 1382 104 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 5457 5603 387
Size: 1064 138 3494 374 146 1280
Fragments arranged by size:
3494 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2460 2664 2777 3116 3248 3452
Size: 393 449 379 204 113 339 132 204
Cuts at: 3452 4559 5156 5943 6211 1239
Size: 1107 597 787 268 1524
Fragments arranged by size:
1524 1107 787 597 449 393 379 339
268 204 204 132 113
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 6496
EcoRI G'AATT_C
Cuts at: 2260 3805 4183 2260
Size: 1545 378 4573
Fragments arranged by size:
4573 1545 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2669 2179
Size: 490 6006
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 5643 1678
Size: 3965 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 2843 2875 3554
Size: 32 135 32 884 32 355 32 679
Cuts at: 3554 3586 3611 3643 5137 5169 5211 5243 1373
Size: 32 25 32 1494 32 42 32 2626
Fragments arranged by size:
2626 1494 884 679 355 135 42 32
32 32 32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3529 3840 4086 669
Size: 988 281 1591 311 246 3079
Fragments arranged by size:
3079 1591 988 311 281 246
HindIII A'AGCT_T
Cuts at: 748 3098 748
Size: 2350 4146
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KpnI G_GTAC'C
Cuts at: 3821 3821
Size: 6496
MfeI C'AATT_G
Cuts at: 4095 4095
Size: 6496
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2442 3036 5610 5794 1305
Size: 1137 594 2574 184 2007
Fragments arranged by size:
2574 2007 1137 594 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3789 10
Size: 54 1425 343 1957 2717
Fragments arranged by size:
2717 1957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2808 3269 4454 4813 4972 518
Size: 648 1642 461 1185 359 159 2042
Fragments arranged by size:
2042 1642 1185 648 461 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 3226 3912 4589 5737 5982 1606
Size: 453 1167 686 677 1148 245 2120
Fragments arranged by size:
2120 1167 1148 686 677 453 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5005
Fragments arranged by size:
5005 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6496
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NsiI A_TGCA'T
Cuts at: 2571 3780 2571
Size: 1209 5287
NspI r_CATG'y
Cuts at: 1132 1229 2573 3773 3854 5399 1132
Size: 97 1344 1200 81 1545 2229
Fragments arranged by size:
2229 1545 1344 1200 97 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6496
PciI A'CATG_T
Cuts at: 5395 5395
Size: 6496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6496
PfoI T'CCnGG_A
Cuts at: 2043 2043
Size: 6496
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PmlI CAC'GTG
Cuts at: 2722 2722
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4565 4597 6078 6110 4565
Size: 32 1481 32 4951
Fragments arranged by size:
4951 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6496
PsiI TTA'TAA
Cuts at: 2541 3150 4066 2541
Size: 609 916 4971
Fragments arranged by size:
4971 916 609
PstI C_TGCA'G
Cuts at: 830 3237 3848 830
Size: 2407 611 3478
Fragments arranged by size:
3478 2407 611
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 2059 3226 2059
Size: 1167 5329
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SapI GCTCTTCn'nnn_
Cuts at: 2865 2865
Size: 6496
SbfI CC_TGCA'GG
Cuts at: 3848 3848
Size: 6496
ScaI AGT'ACT
Cuts at: 4744 6456 4744
Size: 1712 4784
SfcI C'TryA_G
Cuts at: 826 1045 3233 3844 4979 5660 5851 826
Size: 219 2188 611 1135 681 191 1471
Fragments arranged by size:
2188 1471 1135 681 611 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6496
SmaI CCC'GGG
Cuts at: 1872 3823 1872
Size: 1951 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 4600 5501 5763 820
Size: 197 926 711 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 711 262 197
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6496
SphI G_CATG'C
Cuts at: 1132 2573 3854 1132
Size: 1441 1281 3774
Fragments arranged by size:
3774 1441 1281
SspI AAT'ATT
Cuts at: 5 52 3599 4420 5
Size: 47 3547 821 2081
Fragments arranged by size:
3547 2081 821 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6496
StyI C'CwwG_G
Cuts at: 513 1065 2004 3682 3935 513
Size: 552 939 1678 253 3074
Fragments arranged by size:
3074 1678 939 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4523 4540 4693 4878 5323 325
Size: 1255 2943 17 153 185 445 1498
Fragments arranged by size:
2943 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 2269 4742
Size: 274 80 33 51 1443 150 142 2473
Cuts at: 4742 6454 96
Size: 1712 138
Fragments arranged by size:
2473 1712 1443 274 150 142 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2797 2829 2912 2944 3337 3369 6456 6488 2797
Size: 32 83 32 393 32 3087 32 2805
Fragments arranged by size:
3087 2805 393 83 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 2122 3832 2122
Size: 1710 4786
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 2620 2455
Size: 165 6331
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 3821 1870
Size: 1951 4545
XmnI GAAnn'nnTTC
Cuts at: 4625 4625
Size: 6496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4304 276
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI