Restriction map for
pFN21AB6150
(Circular) (Six-base) MAPSORT of: pFN21AB6150.seq Check: 55 from: 1 to: 6625
pFN21AB6150 6625 bp
With 182 enzymes: SgfI *
March 28, 2012 12:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3813 4435 278
Size: 53 83 186 936 2277 622 2468
Fragments arranged by size:
2468 2277 936 622 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3968 1937
Size: 2031 4594
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2526 2664 2880 3702 4688 6072 6340 1632
Size: 894 138 216 822 986 1384 268 1917
Fragments arranged by size:
1917 1384 986 894 822 268 216 138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 2203 5524 6501 1203
Size: 1000 3321 977 1327
Fragments arranged by size:
3321 1327 1000 977
AgeI A'CCGG_T
Cuts at: 2120 5456 2120
Size: 3336 3289
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6625
AlwNI CAG_nnn'CTG
Cuts at: 4368 5940 4368
Size: 1572 5053
ApaI G_GGCC'C
Cuts at: 3672 3672
Size: 6625
ApaLI G'TGCA_C
Cuts at: 3300 4682 5838 3300
Size: 1382 1156 4087
Fragments arranged by size:
4087 1382 1156
ApoI r'AATT_y
Cuts at: 1493 2315 3419 3527 3632 3934 4160 4312 6421
Size: 822 1104 108 105 302 226 152 2109
Cuts at: 6421 1493
Size: 1697
Fragments arranged by size:
2109 1697 1104 822 302 226 152 108
105
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 3950 1870
Size: 73 85 1922 4545
Fragments arranged by size:
4545 1922 85 73
AvrII C'CTAG_G
Cuts at: 3616 3616
Size: 6625
BamHI G'GATC_C
Cuts at: 3307 3955 4322 3307
Size: 648 367 5610
Fragments arranged by size:
5610 648 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3946 5401 618
Size: 325 217 627 2159 1455 1842
Fragments arranged by size:
2159 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 3030 3672 3727 3944 1391
Size: 709 930 642 55 217 4072
Fragments arranged by size:
4072 930 709 642 217 55
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3618 928
Size: 971 1719 3935
Fragments arranged by size:
3935 1719 971
BbvCI CC'TCA_GC
Cuts at: 2040 2040
Size: 6625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4816 4850 1903
Size: 34 2879 34 3678
Fragments arranged by size:
3678 2879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2146 4517 5727 2146
Size: 2371 1210 3044
Fragments arranged by size:
3044 2371 1210
BclI T'GATC_A
Cuts at: 1548 2611 3591 1548
Size: 1063 980 4582
Fragments arranged by size:
4582 1063 980
BfrBI ATG'CAT
Cuts at: 2891 3094 2891
Size: 203 6422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5236 136
Size: 107 122 71 631 605 3564 1525
Fragments arranged by size:
3564 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6620 6620
Size: 6625
BlpI GC'TnA_GC
Cuts at: 3024 3726 4042 3024
Size: 702 316 5607
Fragments arranged by size:
5607 702 316
Bme1580I G_kGCm'C
Cuts at: 1790 3304 3672 4686 5842 1790
Size: 1514 368 1014 1156 2573
Fragments arranged by size:
2573 1514 1156 1014 368
BmgBI CAC'GTC
Cuts at: 1318 2963 1318
Size: 1645 4980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2863 2908 4356
Size: 416 53 83 399 24 1446 45 1448
Cuts at: 4356 5314 442
Size: 958 1753
Fragments arranged by size:
1753 1448 1446 958 416 399 83 53
45 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6625
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3044
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2823 3557 5285 1239
Size: 1584 734 1728 2579
Fragments arranged by size:
2579 1728 1584 734
Bpu10I CC'TnA_GC
Cuts at: 2040 2493 2040
Size: 453 6172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2087 3732 4750 5615 5913 2087
Size: 1645 1018 865 298 2799
Fragments arranged by size:
2799 1645 1018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5288 882
Size: 617 3789 2219
Fragments arranged by size:
3789 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3881 4416 493
Size: 711 2677 535 2702
Fragments arranged by size:
2702 2677 711 535
BsaBI GATnn'nnATC
Cuts at: 2610 3596 6619 2610
Size: 986 3023 2616
Fragments arranged by size:
3023 2616 986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3810 4432 4814 5449
Size: 53 83 186 936 2277 622 382 635
Cuts at: 5449 275
Size: 1451
Fragments arranged by size:
2277 1451 936 635 622 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2120 5058 5456 5730 5877 6288 2120
Size: 2938 398 274 147 411 2457
Fragments arranged by size:
2938 2457 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6400
Fragments arranged by size:
6400 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 1186
Size: 904 5721
BseYI C'CCAG_C
Cuts at: 2429 3188 5828 6324 2429
Size: 759 2640 496 2730
Fragments arranged by size:
2730 2640 759 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3262 1163
Size: 573 1526 4526
Fragments arranged by size:
4526 1526 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2118 3030 3304 3727 4686 4771 5842 2100
Size: 18 912 274 423 959 85 1071 2883
Fragments arranged by size:
2883 1071 959 912 423 274 85 18
BsmI GAATG_Cn'
Cuts at: 2350 2893 4136 4229 2350
Size: 543 1243 93 4746
Fragments arranged by size:
4746 1243 543 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3488 3488
Size: 6625
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2118 3030 3304 3672 3727 3944
Size: 399 310 18 912 274 368 55 217
Cuts at: 3944 4686 4771 5842 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 912 742 399 368 310 274
217 85 55 18
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 2292 4514 6244 2292
Size: 2222 1730 2673
Fragments arranged by size:
2673 2222 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2926 3980 844
Size: 2082 1054 3489
Fragments arranged by size:
3489 2082 1054
BsrBI CCG'CTC
Cuts at: 1394 1823 4512 1394
Size: 429 2689 3507
Fragments arranged by size:
3507 2689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2554 3063 5120 5294 65
Size: 1183 1306 509 2057 174 1396
Fragments arranged by size:
2057 1396 1306 1183 509 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 3549 5269 5456 1700
Size: 110 310 1429 1720 187 2869
Fragments arranged by size:
2869 1720 1429 310 187 110
BsrGI T'GTAC_A
Cuts at: 96 2522 96
Size: 2426 4199
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 2111 4685 5697 2111
Size: 2574 1012 3039
Fragments arranged by size:
3039 2574 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2091 2228 3185 794
Size: 1297 137 957 4234
Fragments arranged by size:
4234 1297 957 137
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstXI CCAn_nnnn'nTGG
Cuts at: 2594 2594
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 3307 3955 4322 4707 4724 6176 6262 6274
Size: 1335 648 367 385 17 1452 86 12
Cuts at: 6274 6620 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1335 648 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3086 3086
Size: 6625
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 3718 4141 4935 4955 3718
Size: 423 794 20 5388
Fragments arranged by size:
5388 794 423 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 2782 3929 2782
Size: 1147 5478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5632 809
Size: 507 4316 1802
Fragments arranged by size:
4316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 4072 4961
Size: 54 1425 50 275 18 219 2023 889
Cuts at: 4961 6590 8
Size: 1629 43
Fragments arranged by size:
2023 1629 1425 889 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3110 3480 3816 3891 4555 1353
Size: 1757 370 336 75 664 3423
Fragments arranged by size:
3423 1757 664 370 336 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2839 5212 5586 5732 387
Size: 1064 138 1250 2373 374 146 1280
Fragments arranged by size:
2373 1280 1250 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2098 3028 3725 2098
Size: 930 697 4998
Fragments arranged by size:
4998 930 697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2526 2664 2823 2880 3557 3702 4688
Size: 393 894 138 159 57 677 145 986
Cuts at: 4688 5285 6072 6340 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 986 894 787 677 597 393 268
159 145 138 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3620 3620
Size: 6625
EcoO109I rG'GnC_Cy
Cuts at: 3668 3668
Size: 6625
EcoRI G'AATT_C
Cuts at: 3934 4312 3934
Size: 378 6247
EcoRV GAT'ATC
Cuts at: 3130 3130
Size: 6625
FspI TGC'GCA
Cuts at: 5131 5131
Size: 6625
HaeII r_GCGC'y
Cuts at: 1678 5772 1678
Size: 4094 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3035 3067 3114 3119 3146
Size: 32 135 32 1463 32 47 5 27
Cuts at: 3146 3151 5266 5298 5340 5372 1373
Size: 5 2115 32 42 32 2626
Fragments arranged by size:
2626 2115 1463 135 47 42 32 32
32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3969 4215 669
Size: 988 281 867 1164 246 3079
Fragments arranged by size:
3079 1164 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 2774 3284 748
Size: 2026 510 4089
Fragments arranged by size:
4089 2026 510
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 3322 4224 3322
Size: 902 5723
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2188 2347 2829 5739 5923 1305
Size: 883 159 482 2910 184 2007
Fragments arranged by size:
2910 2007 883 482 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4803
Fragments arranged by size:
4803 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4583 4942 5101 518
Size: 648 3417 359 159 2042
Fragments arranged by size:
3417 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2399 2429 4041 4718 5866 6111 1606
Size: 793 30 1612 677 1148 245 2120
Fragments arranged by size:
2120 1612 1148 793 677 245 30
NaeI GCC'GGC
Cuts at: 3551 3551
Size: 6625
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
NdeI CA'TA_TG
Cuts at: 387 3032 387
Size: 2645 3980
NgoMIV G'CCGG_C
Cuts at: 3549 3549
Size: 6625
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6625
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NsiI A_TGCA'T
Cuts at: 2893 3096 2893
Size: 203 6422
NspI r_CATG'y
Cuts at: 1132 1229 2207 3983 5528 1132
Size: 97 978 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 978 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 2203 5524 2203
Size: 3321 3304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6625
PfoI T'CCnGG_A
Cuts at: 3573 3573
Size: 6625
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PspOMI G'GGCC_C
Cuts at: 3668 3668
Size: 6625
PstI C_TGCA'G
Cuts at: 830 2250 3977 830
Size: 1420 1727 3478
Fragments arranged by size:
3478 1727 1420
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 2399 2429 2399
Size: 30 6595
SacI G_AGCT'C
Cuts at: 2100 3030 3727 2100
Size: 930 697 4998
Fragments arranged by size:
4998 930 697
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SapI GCTCTTCn'nnn_
Cuts at: 3891 3891
Size: 6625
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 2576 4873 6585 2576
Size: 2297 1712 2616
Fragments arranged by size:
2616 2297 1712
SfcI C'TryA_G
Cuts at: 826 1045 2246 3973 5108 5789 5980 826
Size: 219 1201 1727 1135 681 191 1471
Fragments arranged by size:
1727 1471 1201 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 2030 3952 1872
Size: 158 1922 4545
Fragments arranged by size:
4545 1922 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2102 3747 4729 5630 5892 820
Size: 197 926 159 1645 982 901 262 1553
Fragments arranged by size:
1645 1553 982 926 901 262 197 159
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3983 1132
Size: 2851 3774
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 2922 1517
Size: 1405 5220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2232 3616 4064 513
Size: 552 939 228 1384 448 3074
Fragments arranged by size:
3074 1384 939 552 448 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2486 4652 4669 4822 5007 5452 325
Size: 1255 906 2166 17 153 185 445 1498
Fragments arranged by size:
2166 1498 1255 906 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2242 2522 2574
Size: 274 80 33 51 1443 265 280 52
Cuts at: 2574 2863 4871 6583 96
Size: 289 2008 1712 138
Fragments arranged by size:
2008 1712 1443 289 280 274 265 138
80 52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3426 3458 6585 6617 3426
Size: 32 3127 32 3434
Fragments arranged by size:
3434 3127 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6625
XbaI T'CTAG_A
Cuts at: 2941 3961 2941
Size: 1020 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3452 3452
Size: 6625
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 2028 3950 1870
Size: 158 1922 4545
Fragments arranged by size:
4545 1922 158
XmnI GAAnn'nnTTC
Cuts at: 4754 4754
Size: 6625
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3811 4433 276
Size: 53 83 186 936 2277 622 2468
Fragments arranged by size:
2468 2277 936 622 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BsiWI BstZ17I
DraIII FalI FseI FspAI KasI NarI NruI PacI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI