Restriction map for
pFN21AB6148
(Circular) (Six-base) MAPSORT of: pFN21AB6148.seq Check: 4998 from: 1 to: 6625
pFN21AB6148 6625 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4435 278
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3968 1937
Size: 2031 4594
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 3549 4688 6072 6340 1632
Size: 1040 877 1139 1384 268 1917
Fragments arranged by size:
1917 1384 1139 1040 877 268
AfeI AGC'GCT
Cuts at: 2045 2045
Size: 6625
AflII C'TTAA_G
Cuts at: 820 1017 3473 820
Size: 197 2456 3972
Fragments arranged by size:
3972 2456 197
AflIII A'CryG_T
Cuts at: 1203 5524 6501 1203
Size: 4321 977 1327
Fragments arranged by size:
4321 1327 977
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 2845 5354 2845
Size: 2509 4116
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6625
AlwNI CAG_nnn'CTG
Cuts at: 2537 4368 5940 2537
Size: 1831 1572 3222
Fragments arranged by size:
3222 1831 1572
ApaLI G'TGCA_C
Cuts at: 4682 5838 4682
Size: 1156 5469
ApoI r'AATT_y
Cuts at: 1493 2152 2208 2499 2758 3181 3517 3893 3934
Size: 659 56 291 259 423 336 376 41
Cuts at: 3934 4160 4312 6421 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 659 423 376 336 291 259
226 152 56 41
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 3950 1870
Size: 73 186 1821 4545
Fragments arranged by size:
4545 1821 186 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3247 3280 3247
Size: 33 6592
BamHI G'GATC_C
Cuts at: 3955 4322 3955
Size: 367 6258
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2900 3946 5401 618
Size: 325 217 627 1113 1046 1455 1842
Fragments arranged by size:
1842 1455 1113 1046 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3944 1391
Size: 2553 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5654
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4816 4850 1903
Size: 34 2879 34 3678
Fragments arranged by size:
3678 2879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2819 4517 5727 2819
Size: 1698 1210 3717
Fragments arranged by size:
3717 1698 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6625
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2654 5236
Size: 107 122 71 631 605 337 645 2582
Cuts at: 5236 136
Size: 1525
Fragments arranged by size:
2582 1525 645 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3469 6620 3469
Size: 3151 3474
BlpI GC'TnA_GC
Cuts at: 4042 4042
Size: 6625
Bme1580I G_kGCm'C
Cuts at: 1790 4686 5842 1790
Size: 2896 1156 2573
Fragments arranged by size:
2896 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6625
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4356 5314 442
Size: 416 53 83 399 24 2939 958 1753
Fragments arranged by size:
2939 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2302 2346 2672 3192 3493 3729 5285 1239
Size: 1063 44 326 520 301 236 1556 2579
Fragments arranged by size:
2579 1556 1063 520 326 301 236 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2499 3210 4750 5615 5913 2499
Size: 711 1540 865 298 3211
Fragments arranged by size:
3211 1540 865 711 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5288 882
Size: 617 3789 2219
Fragments arranged by size:
3789 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4416 493
Size: 711 3212 2702
Fragments arranged by size:
3212 2702 711
BsaBI GATnn'nnATC
Cuts at: 6619 6619
Size: 6625
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4432 4814 5449 275
Size: 53 83 186 936 2899 382 635 1451
Fragments arranged by size:
2899 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2668 5058 5456 5730 5877 6288 2668
Size: 2390 398 274 147 411 3005
Fragments arranged by size:
3005 2390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2705 2735 3179 3209 1178
Size: 30 165 30 1302 30 444 30 4594
Fragments arranged by size:
4594 1302 444 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2444 2943 3159 3543 1186
Size: 865 393 499 216 384 4268
Fragments arranged by size:
4268 865 499 393 384 216
BseYI C'CCAG_C
Cuts at: 2068 2334 5828 6324 2068
Size: 266 3494 496 2369
Fragments arranged by size:
3494 2369 496 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6052
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 4686 4771 5842 2865
Size: 1821 85 1071 3648
Fragments arranged by size:
3648 1821 1071 85
BsmI GAATG_Cn'
Cuts at: 2896 4136 4229 2896
Size: 1240 93 5292
Fragments arranged by size:
5292 1240 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2105 2889 2931 3616 2105
Size: 784 42 685 5114
Fragments arranged by size:
5114 784 685 42
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2865 3944 4686 4771 5842 1391
Size: 399 1075 1079 742 85 1071 2174
Fragments arranged by size:
2174 1079 1075 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 3772 4514 6244 3772
Size: 742 1730 4153
Fragments arranged by size:
4153 1730 742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3980 844
Size: 3136 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4512 1394
Size: 429 2689 3507
Fragments arranged by size:
3507 2689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5120 5294 65
Size: 1183 3872 174 1396
Fragments arranged by size:
3872 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5269 5456 1700
Size: 110 3459 187 2869
Fragments arranged by size:
3459 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 2406 4685 5697 2406
Size: 2279 1012 3334
Fragments arranged by size:
3334 2279 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6625
BstBI TT'CG_AA
Cuts at: 2581 5429 2581
Size: 2848 3777
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstXI CCAn_nnnn'nTGG
Cuts at: 2696 2696
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 3469 3955 4322 4707 4724 6176 6262 6274
Size: 1497 486 367 385 17 1452 86 12
Cuts at: 6274 6620 1972
Size: 346 1977
Fragments arranged by size:
1977 1497 1452 486 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2283 2283
Size: 6625
BtgI C'CryG_G
Cuts at: 513 1065 2004 2194 3555 513
Size: 552 939 190 1361 3583
Fragments arranged by size:
3583 1361 939 552 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 3546 4141 4935 4955 3546
Size: 595 794 20 5216
Fragments arranged by size:
5216 794 595 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2452 2487 529
Size: 35 1888 35 4667
Fragments arranged by size:
4667 1888 35 35
DraI TTT'AAA
Cuts at: 2800 3929 2800
Size: 1129 5496
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5632 809
Size: 507 4316 1802
Fragments arranged by size:
4316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4072 4961 6590
Size: 54 1425 50 275 18 2242 889 1629
Cuts at: 6590 8
Size: 43
Fragments arranged by size:
2242 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3477 4555 1353
Size: 2124 1078 3423
Fragments arranged by size:
3423 2124 1078
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5212 5586 5732 387
Size: 1064 138 3623 374 146 1280
Fragments arranged by size:
3623 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2302 2346 2672 3192 3493 3549 3729
Size: 393 670 44 326 520 301 56 180
Cuts at: 3729 4688 5285 6072 6340 1239
Size: 959 597 787 268 1524
Fragments arranged by size:
1524 959 787 670 597 520 393 326
301 268 180 56 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 2033
Size: 6625
EcoO109I rG'GnC_Cy
Cuts at: 2026 2767 2026
Size: 741 5884
EcoRI G'AATT_C
Cuts at: 2758 3934 4312 2758
Size: 1176 378 5071
Fragments arranged by size:
5071 1176 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 6625
FspI TGC'GCA
Cuts at: 5131 5131
Size: 6625
HaeII r_GCGC'y
Cuts at: 1678 2047 2129 3739 5772 1678
Size: 369 82 1610 2033 2531
Fragments arranged by size:
2531 2033 1610 369 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2160 2192 2420 2452 2705
Size: 32 135 32 588 32 228 32 253
Cuts at: 2705 2737 2831 2863 2930 2962 3905 3937 5266
Size: 32 94 32 67 32 943 32 1329
Cuts at: 5266 5298 5340 5372 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 943 588 253 228 135 94
67 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2553 3241 3969 4215 669
Size: 988 281 615 688 728 246 3079
Fragments arranged by size:
3079 988 728 688 615 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6625
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 2341 3570 4224 2341
Size: 1229 654 4742
Fragments arranged by size:
4742 1229 654
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3216 5739 5923 1305
Size: 1911 2523 184 2007
Fragments arranged by size:
2523 2007 1911 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4803
Fragments arranged by size:
4803 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4583 4942 5101 518
Size: 648 3417 359 159 2042
Fragments arranged by size:
3417 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3097 3136 4041 4718 5866 6111 1606
Size: 1491 39 905 677 1148 245 2120
Fragments arranged by size:
2120 1491 1148 905 677 245 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 3555 513
Size: 552 939 1551 3583
Fragments arranged by size:
3583 1551 939 552
NdeI CA'TA_TG
Cuts at: 387 2479 387
Size: 2092 4533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6625
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2099 3683 3983 5528 1132
Size: 97 870 1584 300 1545 2229
Fragments arranged by size:
2229 1584 1545 870 300 97
PasI CC'CwG_GG
Cuts at: 1348 2725 1348
Size: 1377 5248
PciI A'CATG_T
Cuts at: 5524 5524
Size: 6625
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6625
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2767 2767
Size: 6625
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 3636 4195 3636
Size: 559 6066
PstI C_TGCA'G
Cuts at: 830 2899 3088 3977 830
Size: 2069 189 889 3478
Fragments arranged by size:
3478 2069 889 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 4873 6585 4873
Size: 1712 4913
SfcI C'TryA_G
Cuts at: 826 1045 2895 3084 3696 3973 5108 5789 5980
Size: 219 1850 189 612 277 1135 681 191
Cuts at: 5980 826
Size: 1471
Fragments arranged by size:
1850 1471 1135 681 612 277 219 191
189
SfiI GGCCn_nnn'nGGCC
Cuts at: 2654 2654
Size: 6625
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 3952 1872
Size: 2080 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2129 2514 3225 3473 4729 5630
Size: 197 926 186 385 711 248 1256 901
Cuts at: 5630 5892 820
Size: 262 1553
Fragments arranged by size:
1553 1256 926 901 711 385 262 248
197 186
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3683 3983 1132
Size: 2551 300 3774
Fragments arranged by size:
3774 2551 300
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 2657 3090 1517
Size: 1140 433 5052
Fragments arranged by size:
5052 1140 433
StyI C'CwwG_G
Cuts at: 513 1065 2004 2185 3555 4064 513
Size: 552 939 181 1370 509 3074
Fragments arranged by size:
3074 1370 939 552 509 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4652 4669 4822 5007 5452 325
Size: 1255 3072 17 153 185 445 1498
Fragments arranged by size:
3072 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3666 4871 6583
Size: 274 80 33 51 1443 1689 1205 1712
Cuts at: 6583 96
Size: 138
Fragments arranged by size:
1712 1689 1443 1205 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3371 3403 6585 6617 3371
Size: 32 3182 32 3379
Fragments arranged by size:
3379 3182 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6625
XbaI T'CTAG_A
Cuts at: 2867 3961 2867
Size: 1094 5531
XhoI C'TCGA_G
Cuts at: 1943 2129 1943
Size: 186 6439
XmaI C'CCGG_G
Cuts at: 1870 3950 1870
Size: 2080 4545
XmnI GAAnn'nnTTC
Cuts at: 3852 4754 3852
Size: 902 5723
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4433 276
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI