Restriction map for
pFN21AB6142
(Circular) (Six-base) MAPSORT of: pFN21AB6142.seq Check: 3778 from: 1 to: 6619
pFN21AB6142 6619 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3341 3341
Size: 6619
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3962 1937
Size: 2025 4594
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 3918 4746 5119 1563
Size: 2355 828 373 3063
Fragments arranged by size:
3063 2355 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 2472 2554 3052 3776 4682 6066 6334
Size: 582 258 82 498 724 906 1384 268
Cuts at: 6334 1632
Size: 1917
Fragments arranged by size:
1917 1384 906 724 582 498 268 258
82
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 2916 3678 5518 6495 1203
Size: 1713 762 1840 977 1327
Fragments arranged by size:
1840 1713 1327 977 762
AgeI A'CCGG_T
Cuts at: 2086 5450 2086
Size: 3364 3255
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2738 3476 1184
Size: 1554 738 4327
Fragments arranged by size:
4327 1554 738
AlwNI CAG_nnn'CTG
Cuts at: 2269 3238 4362 5934 2269
Size: 969 1124 1572 2954
Fragments arranged by size:
2954 1572 1124 969
ApaI G_GGCC'C
Cuts at: 2816 2847 2816
Size: 31 6588
ApaLI G'TGCA_C
Cuts at: 4676 5832 4676
Size: 1156 5463
ApoI r'AATT_y
Cuts at: 1493 3314 3499 3928 4154 4306 6415 1493
Size: 1821 185 429 226 152 2109 1697
Fragments arranged by size:
2109 1821 1697 429 226 185 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 2847 3092 3167 3696 3944 1870
Size: 73 904 245 75 529 248 4545
Fragments arranged by size:
4545 904 529 248 245 75 73
BamHI G'GATC_C
Cuts at: 3949 4316 3949
Size: 367 6252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3353 3593 3701 3940 5395
Size: 325 217 627 1566 240 108 239 1455
Cuts at: 5395 618
Size: 1842
Fragments arranged by size:
1842 1566 1455 627 325 240 239 217
108
BanII G_rGCy'C
Cuts at: 1391 2816 2847 2856 3938 1391
Size: 1425 31 9 1082 4072
Fragments arranged by size:
4072 1425 1082 31 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2083 2248 3696 928
Size: 971 184 165 1448 3851
Fragments arranged by size:
3851 1448 971 184 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3286 3320 4810 4844 1903
Size: 34 1349 34 1490 34 3678
Fragments arranged by size:
3678 1490 1349 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2462 4511 5721 2462
Size: 2049 1210 3360
Fragments arranged by size:
3360 2049 1210
BclI T'GATC_A
Cuts at: 1548 2400 1548
Size: 852 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2898 3104 3170
Size: 107 122 71 631 605 1226 206 66
Cuts at: 3170 5230 136
Size: 2060 1525
Fragments arranged by size:
2060 1525 1226 631 605 206 122 107
71 66
BglII A'GATC_T
Cuts at: 6614 6614
Size: 6619
BlpI GC'TnA_GC
Cuts at: 2079 3611 4036 2079
Size: 1532 425 4662
Fragments arranged by size:
4662 1532 425
Bme1580I G_kGCm'C
Cuts at: 1790 2816 2847 3596 3796 4680 5836 1790
Size: 1026 31 749 200 884 1156 2573
Fragments arranged by size:
2573 1156 1026 884 749 200 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6619
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2020 2418 4350
Size: 416 53 83 399 24 603 398 1932
Cuts at: 4350 5308 442
Size: 958 1753
Fragments arranged by size:
1932 1753 958 603 416 399 398 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 3061 2779
Size: 282 6337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2959 3218 3536 3734 3881 5279 1239
Size: 1052 668 259 318 198 147 1398 2579
Fragments arranged by size:
2579 1398 1052 668 318 259 198 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4744 5609 5907 4744
Size: 865 298 5456
Fragments arranged by size:
5456 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2271 3555 5282 882
Size: 617 772 1284 1727 2219
Fragments arranged by size:
2219 1727 1284 772 617
BsaAI yAC'GTr
Cuts at: 493 1204 2917 3681 4410 493
Size: 711 1713 764 729 2702
Fragments arranged by size:
2702 1713 764 729 711
BsaBI GATnn'nnATC
Cuts at: 2357 2792 6613 2357
Size: 435 3821 2363
Fragments arranged by size:
3821 2363 435
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3480 3619 3693 4426
Size: 53 83 186 936 1947 139 74 733
Cuts at: 4426 4808 5443 275
Size: 382 635 1451
Fragments arranged by size:
1947 1451 936 733 635 382 186 139
83 74 53
BsaWI w'CCGG_w
Cuts at: 2086 2718 5052 5450 5724 5871 6282 2086
Size: 632 2334 398 274 147 411 2423
Fragments arranged by size:
2423 2334 632 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2522 2552 2673 2703 3405
Size: 30 165 30 1119 30 121 30 702
Cuts at: 3405 3435 1178
Size: 30 4362
Fragments arranged by size:
4362 1119 702 165 121 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2752 3573 3869 1186
Size: 1566 821 296 3936
Fragments arranged by size:
3936 1566 821 296
BseYI C'CCAG_C
Cuts at: 2464 2808 3718 5822 6318 2464
Size: 344 910 2104 496 2765
Fragments arranged by size:
2765 2104 910 496 344
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2661 3115 3443 1163
Size: 573 925 454 328 4339
Fragments arranged by size:
4339 925 573 454 328
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2087 4069 4830 5858 1540
Size: 275 186 39 47 1982 761 1028 2301
Fragments arranged by size:
2301 1982 1028 761 275 186 47 39
BsiHKAI G_wGCw'C
Cuts at: 2856 3249 4680 4765 5836 2856
Size: 393 1431 85 1071 3639
Fragments arranged by size:
3639 1431 1071 393 85
BsmI GAATG_Cn'
Cuts at: 3142 4130 4223 3142
Size: 988 93 5538
Fragments arranged by size:
5538 988 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2816 2847 2856 3249 3596 3796 3938
Size: 399 1026 31 9 393 347 200 142
Cuts at: 3938 4680 4765 5836 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1026 742 399 393 347 200
142 85 31 9
BspEI T'CCGG_A
Cuts at: 2718 6282 2718
Size: 3564 3055
BspHI T'CATG_A
Cuts at: 2739 3666 4508 6238 2739
Size: 927 842 1730 3120
Fragments arranged by size:
3120 1730 927 842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3341 3974 844
Size: 2497 633 3489
Fragments arranged by size:
3489 2497 633
BsrBI CCG'CTC
Cuts at: 1394 1823 4506 1394
Size: 429 2683 3507
Fragments arranged by size:
3507 2683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3304 5114 5288 65
Size: 1183 2056 1810 174 1396
Fragments arranged by size:
2056 1810 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2086 3098 3615 5263 5450 1700
Size: 110 276 1012 517 1648 187 2869
Fragments arranged by size:
2869 1648 1012 517 276 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6619
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 4679 5691 4679
Size: 1012 5607
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6619
BstBI TT'CG_AA
Cuts at: 5423 5423
Size: 6619
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6619
BstYI r'GATC_y
Cuts at: 1972 3949 4316 4701 4718 6170 6256 6268 6614
Size: 1977 367 385 17 1452 86 12 346
Cuts at: 6614 1972
Size: 1977
Fragments arranged by size:
1977 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2626 2868 2893 3357 3882 513
Size: 552 1561 242 25 464 525 3250
Fragments arranged by size:
3250 1561 552 525 464 242 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 2396 4135 4929 4949 2396
Size: 1739 794 20 4066
Fragments arranged by size:
4066 1739 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2659 2694 529
Size: 35 2095 35 4454
Fragments arranged by size:
4454 2095 35 35
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DraIII CAC_nnn'GTG
Cuts at: 3409 3409
Size: 6619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2871 3837
Size: 54 1425 50 275 18 207 834 966
Cuts at: 3837 4066 4955 6584 8
Size: 229 889 1629 43
Fragments arranged by size:
1629 1425 966 889 834 275 229 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2037 4066 1537
Size: 275 225 2029 4090
Fragments arranged by size:
4090 2029 275 225
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 3052 3550 3861 4549 1353
Size: 704 995 498 311 688 3423
Fragments arranged by size:
3423 995 704 688 498 311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2854 2854
Size: 6619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2291 2472 2554 2959 3052 3218
Size: 393 582 77 181 82 405 93 166
Cuts at: 3218 3536 3734 3776 3881 4682 5279 6066 6334
Size: 318 198 42 105 801 597 787 268
Cuts at: 6334 1239
Size: 1524
Fragments arranged by size:
1524 801 787 597 582 405 393 318
268 198 181 166 105 93 82 77
42
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 2228
Size: 6619
EcoO109I rG'GnC_Cy
Cuts at: 2631 2812 2813 2844 3340 3427 2631
Size: 181 1 31 496 87 5823
Fragments arranged by size:
5823 496 181 87 31 1
EcoRI G'AATT_C
Cuts at: 3928 4306 3928
Size: 378 6241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 3664 2203
Size: 1461 5158
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 2805 3738 5766 1678
Size: 1127 933 2028 2531
Fragments arranged by size:
2531 2028 1127 933
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2503 2522 2535 2554 3604
Size: 32 135 32 931 19 13 19 1050
Cuts at: 3604 3620 3636 3652 3812 3844 5260 5292 5334
Size: 16 16 16 160 32 1416 32 42
Cuts at: 5334 5366 1373
Size: 32 2626
Fragments arranged by size:
2626 1416 1050 931 160 135 42 32
32 32 32 32 19 19 16 16
16 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3544 3963 4209 669
Size: 988 281 1606 419 246 3079
Fragments arranged by size:
3079 1606 988 419 281 246
HindIII A'AGCT_T
Cuts at: 748 2180 748
Size: 1432 5187
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 2665 5733 5917 1305
Size: 1260 100 3068 184 2007
Fragments arranged by size:
3068 2007 1260 184 100
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3839 10
Size: 54 1425 343 2007 2790
Fragments arranged by size:
2790 2007 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2720 2915 3575 4577 4936 5095
Size: 648 1197 357 195 660 1002 359 159
Cuts at: 5095 518
Size: 2042
Fragments arranged by size:
2042 1197 1002 660 648 359 357 195
159
MspA1I CmG'CkG
Cuts at: 1606 2269 2785 2808 2895 3161 3176 4035 4712
Size: 663 516 23 87 266 15 859 677
Cuts at: 4712 5860 6105 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 859 677 663 516 266 245
87 23 15
NaeI GCC'GGC
Cuts at: 3617 3617
Size: 6619
NcoI C'CATG_G
Cuts at: 513 1065 2868 3357 513
Size: 552 1803 489 3775
Fragments arranged by size:
3775 1803 552 489
NdeI CA'TA_TG
Cuts at: 387 2775 387
Size: 2388 4231
NgoMIV G'CCGG_C
Cuts at: 3615 3615
Size: 6619
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 2037 4066 2037
Size: 2029 4590
NspI r_CATG'y
Cuts at: 1132 1229 2983 3232 3524 3977 5522 1132
Size: 97 1754 249 292 453 1545 2229
Fragments arranged by size:
2229 1754 1545 453 292 249 97
PasI CC'CwG_GG
Cuts at: 1348 2283 1348
Size: 935 5684
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2982 3409 3449 1108
Size: 1874 427 40 4278
Fragments arranged by size:
4278 1874 427 40
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PmlI CAC'GTG
Cuts at: 3681 3681
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4688 4720 6201 6233 4688
Size: 32 1481 32 5074
Fragments arranged by size:
5074 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2631 3340 3427 2631
Size: 709 87 5823
Fragments arranged by size:
5823 709 87
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PspOMI G'GGCC_C
Cuts at: 2812 2843 2812
Size: 31 6588
PstI C_TGCA'G
Cuts at: 830 2865 3160 3971 830
Size: 2035 295 811 3478
Fragments arranged by size:
3478 2035 811 295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 2269 2785 2808 3161 3176 2269
Size: 516 23 353 15 5712
Fragments arranged by size:
5712 516 353 23 15
SacI G_AGCT'C
Cuts at: 2856 2856
Size: 6619
SacII CC_GC'GG
Cuts at: 2896 2896
Size: 6619
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SanDI GG'GwC_CC
Cuts at: 2631 2631
Size: 6619
SapI GCTCTTCn'nnn_
Cuts at: 3052 3861 3052
Size: 809 5810
SbfI CC_TGCA'GG
Cuts at: 3971 3971
Size: 6619
ScaI AGT'ACT
Cuts at: 4867 6579 4867
Size: 1712 4907
SexAI A'CCwGG_T
Cuts at: 3807 3807
Size: 6619
SfcI C'TryA_G
Cuts at: 826 1045 2797 2861 3156 3967 5102 5783 5974
Size: 219 1752 64 295 811 1135 681 191
Cuts at: 5974 826
Size: 1471
Fragments arranged by size:
1752 1471 1135 811 681 295 219 191
64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 2849 3946 1872
Size: 977 1097 4545
Fragments arranged by size:
4545 1097 977
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4723 5624 5886 820
Size: 197 926 2780 901 262 1553
Fragments arranged by size:
2780 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 4543 5
Size: 47 4491 2081
Fragments arranged by size:
4491 2081 47
StuI AGG'CCT
Cuts at: 1517 2759 1517
Size: 1242 5377
StyI C'CwwG_G
Cuts at: 513 1065 2703 2868 3054 3357 3623 3639 4058
Size: 552 1638 165 186 303 266 16 419
Cuts at: 4058 513
Size: 3074
Fragments arranged by size:
3074 1638 552 419 303 266 186 165
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2334 4646 4663 4816 5001 5446 325
Size: 1255 754 2312 17 153 185 445 1498
Fragments arranged by size:
2312 1498 1255 754 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4865 6577 96
Size: 274 80 33 51 1443 2888 1712 138
Fragments arranged by size:
2888 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2623 2655 3405 3437 6579 6611 2623
Size: 32 750 32 3142 32 2631
Fragments arranged by size:
3142 2631 750 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3278 1316
Size: 1962 4657
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 2497 3364 3454 2290
Size: 207 867 90 5455
Fragments arranged by size:
5455 867 207 90
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 2847 3944 1870
Size: 977 1097 4545
Fragments arranged by size:
4545 1097 977
XmnI GAAnn'nnTTC
Cuts at: 4748 4748
Size: 6619
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PspXI
PsrI RsrII SfiI SfoI SrfI SwaI