Restriction map for
pFN21AB6133
(Circular) (Six-base) MAPSORT of: pFN21AB6133.seq Check: 1013 from: 1 to: 6475
pFN21AB6133 6475 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3818 1937
Size: 1881 4594
Acc65I G'GTAC_C
Cuts at: 3796 3796
Size: 6475
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2236 2849 3646 4538 5922 6190 1632
Size: 406 198 613 797 892 1384 268 1917
Fragments arranged by size:
1917 1384 892 797 613 406 268 198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 5374 6351 1203
Size: 4171 977 1327
Fragments arranged by size:
4171 1327 977
AgeI A'CCGG_T
Cuts at: 2259 5306 2259
Size: 3047 3428
AhdI GACnn_n'nnGTC
Cuts at: 5204 5204
Size: 6475
AleI CACnn'nnGTG
Cuts at: 1166 2105 2969 1166
Size: 939 864 4672
Fragments arranged by size:
4672 939 864
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 6443
AlwNI CAG_nnn'CTG
Cuts at: 2647 2752 3190 4218 5790 2647
Size: 105 438 1028 1572 3332
Fragments arranged by size:
3332 1572 1028 438 105
ApaLI G'TGCA_C
Cuts at: 4532 5688 4532
Size: 1156 5319
ApoI r'AATT_y
Cuts at: 1493 3731 3784 4010 4162 6271 1493
Size: 2238 53 226 152 2109 1697
Fragments arranged by size:
2238 2109 1697 226 152 53
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 3800 1870
Size: 73 1857 4545
Fragments arranged by size:
4545 1857 73
AvrII C'CTAG_G
Cuts at: 3628 3628
Size: 6475
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2782 2815 2782
Size: 33 6442
BamHI G'GATC_C
Cuts at: 3805 4172 3805
Size: 367 6108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2624 3796 5251 618
Size: 325 217 627 516 321 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 516 325 321 217
BanII G_rGCy'C
Cuts at: 1391 2216 2355 3372 3642 3656 3794 1391
Size: 825 139 1017 270 14 138 4072
Fragments arranged by size:
4072 1017 825 270 139 138 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2766 928
Size: 971 867 4637
Fragments arranged by size:
4637 971 867
BbvCI CC'TCA_GC
Cuts at: 3024 3024
Size: 6475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4666 4700 1903
Size: 34 2729 34 3678
Fragments arranged by size:
3678 2729 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2432 4367 5577 2432
Size: 1935 1210 3330
Fragments arranged by size:
3330 1935 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3129 5086 136
Size: 107 122 71 631 605 1457 1957 1525
Fragments arranged by size:
1957 1525 1457 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3159 6470 3159
Size: 3311 3164
BlpI GC'TnA_GC
Cuts at: 3892 3892
Size: 6475
Bme1580I G_kGCm'C
Cuts at: 1790 2627 4536 5692 1790
Size: 837 1909 1156 2573
Fragments arranged by size:
2573 1909 1156 837
BmgBI CAC'GTC
Cuts at: 1318 3397 1318
Size: 2079 4396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3031 4206 5164
Size: 416 53 83 399 24 1614 1175 958
Cuts at: 5164 442
Size: 1753
Fragments arranged by size:
1753 1614 1175 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 2436 2591 2669 2891 3329 3446 3589
Size: 1059 138 155 78 222 438 117 143
Cuts at: 3589 3706 5135 1239
Size: 117 1429 2579
Fragments arranged by size:
2579 1429 1059 438 222 155 143 138
117 117 78
Bpu10I CC'TnA_GC
Cuts at: 3024 3024
Size: 6475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3263 4600 5465 5763 3263
Size: 1337 865 298 3975
Fragments arranged by size:
3975 1337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5138 882
Size: 617 3639 2219
Fragments arranged by size:
3639 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4266 493
Size: 711 3062 2702
Fragments arranged by size:
3062 2702 711
BsaBI GATnn'nnATC
Cuts at: 2228 6469 2228
Size: 4241 2234
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4282 4664 5299 275
Size: 53 83 186 936 2749 382 635 1451
Fragments arranged by size:
2749 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2031 2259 3174 4908 5306 5580 5727 6138 2031
Size: 228 915 1734 398 274 147 411 2368
Fragments arranged by size:
2368 1734 915 411 398 274 228 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2642 2672 3444 3474 1178
Size: 30 165 30 1239 30 772 30 4179
Fragments arranged by size:
4179 1239 772 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 3059 3116 3326 3739 3742 1186
Size: 1333 540 57 210 413 3 3919
Fragments arranged by size:
3919 1333 540 413 210 57 3
BseYI C'CCAG_C
Cuts at: 2480 3685 5678 6174 2480
Size: 1205 1993 496 2781
Fragments arranged by size:
2781 1993 1205 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5902
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3925 4686 5714 1540
Size: 275 186 1924 761 1028 2301
Fragments arranged by size:
2301 1924 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3372 4536 4621 5692 3372
Size: 1164 85 1071 4155
Fragments arranged by size:
4155 1164 1071 85
BsmI GAATG_Cn'
Cuts at: 3443 3986 4079 3443
Size: 543 93 5839
Fragments arranged by size:
5839 543 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2355 2627 3372 3642 3656 3794
Size: 399 426 139 272 745 270 14 138
Cuts at: 3794 4536 4621 5692 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 745 742 426 399 272 270
139 138 85 14
BspEI T'CCGG_A
Cuts at: 2031 6138 2031
Size: 4107 2368
BspHI T'CATG_A
Cuts at: 2910 3372 4364 6094 2910
Size: 462 992 1730 3291
Fragments arranged by size:
3291 1730 992 462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 3830 844
Size: 1631 1355 3489
Fragments arranged by size:
3489 1631 1355
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 4362 1394
Size: 429 214 2325 3507
Fragments arranged by size:
3507 2325 429 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 3243 4970 5144 65
Size: 1183 1995 1727 174 1396
Fragments arranged by size:
1995 1727 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2259 2306 3123 5119 5306 1700
Size: 110 449 47 817 1996 187 2869
Fragments arranged by size:
2869 1996 817 449 187 110 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6475
BssSI C'ACGA_G
Cuts at: 4535 5547 4535
Size: 1012 5463
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6475
BstBI TT'CG_AA
Cuts at: 5279 5279
Size: 6475
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6475
BstYI r'GATC_y
Cuts at: 1972 3159 3805 4172 4557 4574 6026 6112 6124
Size: 1187 646 367 385 17 1452 86 12
Cuts at: 6124 6470 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1187 646 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2681 2681
Size: 6475
BtgI C'CryG_G
Cuts at: 513 1065 2982 513
Size: 552 1917 4006
Fragments arranged by size:
4006 1917 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3004 3147 487
Size: 683 56 1778 143 3815
Fragments arranged by size:
3815 1778 683 143 56
BtsI GCAGTG_nn'
Cuts at: 2802 3499 3520 3526 3595 3619 3679 3709 3991
Size: 697 21 6 69 24 60 30 282
Cuts at: 3991 4785 4805 2802
Size: 794 20 4472
Fragments arranged by size:
4472 794 697 282 69 60 30 24
21 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DraIII CAC_nnn'GTG
Cuts at: 2601 2601
Size: 6475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5482 809
Size: 507 4166 1802
Fragments arranged by size:
4166 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 3300 3676
Size: 54 1425 50 275 18 1291 179 376
Cuts at: 3676 3922 4811 6440 8
Size: 246 889 1629 43
Fragments arranged by size:
1629 1425 1291 889 376 275 246 179
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3922 1537
Size: 275 2110 4090
Fragments arranged by size:
4090 2110 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3755 4405 1353
Size: 2402 650 3423
Fragments arranged by size:
3423 2402 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2376 5062 5436 5582 387
Size: 1064 138 787 2686 374 146 1280
Fragments arranged by size:
2686 1280 1064 787 374 146 138
EcoICRI GAG'CTC
Cuts at: 3370 3370
Size: 6475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2236 2298 2436 2591 2669 2849
Size: 393 406 198 62 138 155 78 180
Cuts at: 2849 2891 3329 3446 3589 3646 3706 4538 5135
Size: 42 438 117 143 57 60 832 597
Cuts at: 5135 5922 6190 1239
Size: 787 268 1524
Fragments arranged by size:
1524 832 787 597 438 406 393 268
198 180 155 143 138 117 78 62
60 57 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2415 2612 3323 2415
Size: 197 711 5567
Fragments arranged by size:
5567 711 197
EcoO109I rG'GnC_Cy
Cuts at: 2749 3316 2749
Size: 567 5908
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
EcoRV GAT'ATC
Cuts at: 2226 2226
Size: 6475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 6475
FspI TGC'GCA
Cuts at: 4981 4981
Size: 6475
HaeII r_GCGC'y
Cuts at: 1678 5622 1678
Size: 3944 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5116 5148 5190 5222 1373
Size: 32 135 32 3544 32 42 32 2626
Fragments arranged by size:
3544 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2440 3472 3819 4065 669
Size: 988 281 502 1032 347 246 3079
Fragments arranged by size:
3079 1032 988 502 347 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6475
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KpnI G_GTAC'C
Cuts at: 3800 3800
Size: 6475
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3442 5589 5773 1305
Size: 2137 2147 184 2007
Fragments arranged by size:
2147 2137 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4653
Fragments arranged by size:
4653 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2105 2915 2969 3132 4433 4792 4951
Size: 648 939 810 54 163 1301 359 159
Cuts at: 4951 518
Size: 2042
Fragments arranged by size:
2042 1301 939 810 648 359 163 159
54
MspA1I CmG'CkG
Cuts at: 1606 2692 3119 3891 4568 5716 5961 1606
Size: 1086 427 772 677 1148 245 2120
Fragments arranged by size:
2120 1148 1086 772 677 427 245
NaeI GCC'GGC
Cuts at: 3125 3125
Size: 6475
NcoI C'CATG_G
Cuts at: 513 1065 2982 513
Size: 552 1917 4006
Fragments arranged by size:
4006 1917 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NgoMIV G'CCGG_C
Cuts at: 3123 3123
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 2746 2957 3833 5378 1132
Size: 97 1517 211 876 1545 2229
Fragments arranged by size:
2229 1545 1517 876 211 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6475
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6475
PfoI T'CCnGG_A
Cuts at: 2413 2568 3755 2413
Size: 155 1187 5133
Fragments arranged by size:
5133 1187 155
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 4544 4576 6057 6089 2642
Size: 32 1870 32 1481 32 3028
Fragments arranged by size:
3028 1870 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2749 2749
Size: 6475
PshAI GACnn'nnGTC
Cuts at: 1613 2954 1613
Size: 1341 5134
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PstI C_TGCA'G
Cuts at: 830 2616 3111 3237 3324 3827 830
Size: 1786 495 126 87 503 3478
Fragments arranged by size:
3478 1786 503 495 126 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 2692 3119 2692
Size: 427 6048
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 6475
SacI G_AGCT'C
Cuts at: 3372 3372
Size: 6475
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SapI GCTCTTCn'nnn_
Cuts at: 3755 3755
Size: 6475
SbfI CC_TGCA'GG
Cuts at: 3827 3827
Size: 6475
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SexAI A'CCwGG_T
Cuts at: 2751 2751
Size: 6475
SfcI C'TryA_G
Cuts at: 826 1045 2612 3107 3233 3320 3823 4958 5639
Size: 219 1567 495 126 87 503 1135 681
Cuts at: 5639 5830 826
Size: 191 1471
Fragments arranged by size:
1567 1471 1135 681 503 495 219 191
126 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6475
SmaI CCC'GGG
Cuts at: 1872 3802 1872
Size: 1930 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3278 4579 5480 5742 820
Size: 197 926 1335 1301 901 262 1553
Fragments arranged by size:
1553 1335 1301 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4266 493
Size: 3773 2702
SpeI A'CTAG_T
Cuts at: 152 2237 152
Size: 2085 4390
SphI G_CATG'C
Cuts at: 1132 3833 1132
Size: 2701 3774
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6475
StyI C'CwwG_G
Cuts at: 513 1065 2877 2982 3628 3914 513
Size: 552 1812 105 646 286 3074
Fragments arranged by size:
3074 1812 646 552 286 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 2516 4502 4519 4672 4857 5302
Size: 1255 585 351 1986 17 153 185 445
Cuts at: 5302 325
Size: 1498
Fragments arranged by size:
1986 1498 1255 585 445 351 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 4721 6433
Size: 274 80 33 51 1443 690 2054 1712
Cuts at: 6433 96
Size: 138
Fragments arranged by size:
2054 1712 1443 690 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2585 2617 6435 6467 2585
Size: 32 3818 32 2593
Fragments arranged by size:
3818 2593 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3209 1316
Size: 1893 4582
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3295 3337 3388 3295
Size: 42 51 6382
Fragments arranged by size:
6382 51 42
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6475
XmaI C'CCGG_G
Cuts at: 1870 3800 1870
Size: 1930 4545
XmnI GAAnn'nnTTC
Cuts at: 2918 3764 4604 2918
Size: 846 840 4789
Fragments arranged by size:
4789 846 840
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI SacII SanDI
SfiI SfoI SrfI SwaI