Restriction map for
pFN21AB6129
(Circular) (Six-base) MAPSORT of: pFN21AB6129.seq Check: 2425 from: 1 to: 6466
pFN21AB6129 6466 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3168 3168
Size: 6466
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4276 278
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2310 3809 1937
Size: 373 1499 4594
Fragments arranged by size:
4594 1499 373
Acc65I G'GTAC_C
Cuts at: 3787 3787
Size: 6466
AclI AA'CG_TT
Cuts at: 1563 4593 4966 1563
Size: 3030 373 3063
Fragments arranged by size:
3063 3030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 3440 4529 5913 6181 1632
Size: 597 1211 1089 1384 268 1917
Fragments arranged by size:
1917 1384 1211 1089 597 268
AflII C'TTAA_G
Cuts at: 820 1017 3596 820
Size: 197 2579 3690
Fragments arranged by size:
3690 2579 197
AflIII A'CryG_T
Cuts at: 1203 2784 3721 5365 6342 1203
Size: 1581 937 1644 977 1327
Fragments arranged by size:
1644 1581 1327 977 937
AgeI A'CCGG_T
Cuts at: 5297 5297
Size: 6466
AhdI GACnn_n'nnGTC
Cuts at: 5195 5195
Size: 6466
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6466
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 6434
AlwNI CAG_nnn'CTG
Cuts at: 2884 4209 5781 2884
Size: 1325 1572 3569
Fragments arranged by size:
3569 1572 1325
ApaLI G'TGCA_C
Cuts at: 4523 5679 4523
Size: 1156 5310
ApoI r'AATT_y
Cuts at: 1493 2541 3775 4001 4153 6262 1493
Size: 1048 1234 226 152 2109 1697
Fragments arranged by size:
2109 1697 1234 1048 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 2315 2520 2814 3791 1870
Size: 73 372 205 294 977 4545
Fragments arranged by size:
4545 977 372 294 205 73
BamHI G'GATC_C
Cuts at: 3069 3796 4163 3069
Size: 727 367 5372
Fragments arranged by size:
5372 727 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3787 5242 618
Size: 325 217 627 2000 1455 1842
Fragments arranged by size:
2000 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2158 3549 3648 3661 3785 1391
Size: 767 1391 99 13 124 4072
Fragments arranged by size:
4072 1391 767 124 99 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2235 2262 2507 3404 928
Size: 971 336 27 245 897 3990
Fragments arranged by size:
3990 971 897 336 245 27
BbvCI CC'TCA_GC
Cuts at: 2084 2221 3275 3694 2084
Size: 137 1054 419 4856
Fragments arranged by size:
4856 1054 419 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2690 2724 3272 3306 4657
Size: 34 50 34 669 34 548 34 1351
Cuts at: 4657 4691 1903
Size: 34 3678
Fragments arranged by size:
3678 1351 669 548 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4358 5568 4358
Size: 1210 5256
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6466
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5077 136
Size: 107 122 71 631 605 3405 1525
Fragments arranged by size:
3405 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6461 6461
Size: 6466
BlpI GC'TnA_GC
Cuts at: 2457 3883 2457
Size: 1426 5040
Bme1580I G_kGCm'C
Cuts at: 1790 2101 4527 5683 1790
Size: 311 2426 1156 2573
Fragments arranged by size:
2573 2426 1156 311
BmgBI CAC'GTC
Cuts at: 1318 2748 3325 1318
Size: 1430 577 4459
Fragments arranged by size:
4459 1430 577
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2124 3520 4197
Size: 416 53 83 399 24 707 1396 677
Cuts at: 4197 5155 442
Size: 958 1753
Fragments arranged by size:
1753 1396 958 707 677 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 3031 5126 1239
Size: 919 873 2095 2579
Fragments arranged by size:
2579 2095 919 873
Bpu10I CC'TnA_GC
Cuts at: 2084 2221 3275 3647 3694 2084
Size: 137 1054 372 47 4856
Fragments arranged by size:
4856 1054 372 137 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4591 5456 5754 4591
Size: 865 298 5303
Fragments arranged by size:
5303 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2046 2312 2696 3049 5129 882
Size: 617 547 266 384 353 2080 2219
Fragments arranged by size:
2219 2080 617 547 384 353 266
BsaAI yAC'GTr
Cuts at: 493 1204 4257 493
Size: 711 3053 2702
Fragments arranged by size:
3053 2702 711
BsaBI GATnn'nnATC
Cuts at: 6460 6460
Size: 6466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4273 4655 5290 275
Size: 53 83 186 936 2740 382 635 1451
Fragments arranged by size:
2740 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4899 5297 5571 5718 6129 4899
Size: 398 274 147 411 5236
Fragments arranged by size:
5236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 2930 2960 1178
Size: 30 165 30 822 30 675 30 4684
Fragments arranged by size:
4684 822 675 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2217 2588 2842 1186
Size: 1031 371 254 4810
Fragments arranged by size:
4810 1031 371 254
BseYI C'CCAG_C
Cuts at: 3660 5669 6165 3660
Size: 2009 496 3961
Fragments arranged by size:
3961 2009 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2113 2976 1163
Size: 573 318 59 863 4653
Fragments arranged by size:
4653 863 573 318 59
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3916 4677 5705 1540
Size: 275 186 1915 761 1028 2301
Fragments arranged by size:
2301 1915 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2158 4527 4612 5683 2158
Size: 2369 85 1071 2941
Fragments arranged by size:
2941 2369 1071 85
BsmI GAATG_Cn'
Cuts at: 3977 4070 3977
Size: 93 6373
BsmBI CGTCTCn'nnnn_
Cuts at: 3302 3302
Size: 6466
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2158 3549 3648 3661 3785 4527
Size: 399 311 57 1391 99 13 124 742
Cuts at: 4527 4612 5683 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1391 1071 742 399 311 124 99
85 57 13
BspEI T'CCGG_A
Cuts at: 6129 6129
Size: 6466
BspHI T'CATG_A
Cuts at: 2274 3127 4355 6085 2274
Size: 853 1228 1730 2655
Fragments arranged by size:
2655 1730 1228 853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 3168 3821 844
Size: 1553 771 653 3489
Fragments arranged by size:
3489 1553 771 653
BsrBI CCG'CTC
Cuts at: 1394 1823 2517 4353 1394
Size: 429 694 1836 3507
Fragments arranged by size:
3507 1836 694 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3288 4961 5135 65
Size: 1183 2040 1673 174 1396
Fragments arranged by size:
2040 1673 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5110 5297 1700
Size: 110 3300 187 2869
Fragments arranged by size:
3300 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6466
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6466
BssSI C'ACGA_G
Cuts at: 4526 5538 4526
Size: 1012 5454
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6466
BstBI TT'CG_AA
Cuts at: 5270 5270
Size: 6466
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6466
BstYI r'GATC_y
Cuts at: 1972 3055 3069 3153 3796 4163 4548 4565 6017
Size: 1083 14 84 643 367 385 17 1452
Cuts at: 6017 6103 6115 6461 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1083 643 385 367 346 86
84 17 14 12
Bsu36I CC'TnA_GG
Cuts at: 2229 2229
Size: 6466
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5727
Fragments arranged by size:
5727 683 56
BtsI GCAGTG_nn'
Cuts at: 2964 3982 4776 4796 2964
Size: 1018 794 20 4634
Fragments arranged by size:
4634 1018 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6431
DraI TTT'AAA
Cuts at: 2211 3770 2211
Size: 1559 4907
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5473 809
Size: 507 4157 1802
Fragments arranged by size:
4157 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3913 4802 6431
Size: 54 1425 50 275 18 2083 889 1629
Cuts at: 6431 8
Size: 43
Fragments arranged by size:
2083 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3913 1537
Size: 275 2101 4090
Fragments arranged by size:
4090 2101 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2372 3432 3757 4396 1353
Size: 1019 1060 325 639 3423
Fragments arranged by size:
3423 1060 1019 639 325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5053 5427 5573 387
Size: 1064 138 3464 374 146 1280
Fragments arranged by size:
3464 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2156 2156
Size: 6466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2229 3031 3440 4529 5126 5913
Size: 393 526 71 802 409 1089 597 787
Cuts at: 5913 6181 1239
Size: 268 1524
Fragments arranged by size:
1524 1089 802 787 597 526 409 393
268 71
EcoNI CCTnn'n_nnAGG
Cuts at: 2408 2408
Size: 6466
EcoO109I rG'GnC_Cy
Cuts at: 2632 2632
Size: 6466
EcoRI G'AATT_C
Cuts at: 2541 3775 4153 2541
Size: 1234 378 4854
Fragments arranged by size:
4854 1234 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2915 3032 3240 3622 2915
Size: 117 208 382 5759
Fragments arranged by size:
5759 382 208 117
FspI TGC'GCA
Cuts at: 4972 4972
Size: 6466
HaeII r_GCGC'y
Cuts at: 1678 2739 5613 1678
Size: 1061 2874 2531
Fragments arranged by size:
2874 2531 1061
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2225 2257 3029
Size: 32 135 32 584 32 37 32 772
Cuts at: 3029 3061 3126 3158 3711 3743 5107 5139 5181
Size: 32 65 32 553 32 1364 32 42
Cuts at: 5181 5213 1373
Size: 32 2626
Fragments arranged by size:
2626 1364 772 584 553 135 65 42
37 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3810 4056 669
Size: 988 281 1872 246 3079
Fragments arranged by size:
3079 1872 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2867 3222 748
Size: 2119 355 3992
Fragments arranged by size:
3992 2119 355
HpaI GTT'AAC
Cuts at: 4056 4056
Size: 6466
KpnI G_GTAC'C
Cuts at: 3791 3791
Size: 6466
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 6342 6342
Size: 6466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3171 5580 5764 1305
Size: 1866 2409 184 2007
Fragments arranged by size:
2409 2007 1866 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4644
Fragments arranged by size:
4644 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4424 4783 4942 518
Size: 648 3258 359 159 2042
Fragments arranged by size:
3258 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3121 3882 4559 5707 5952 1606
Size: 1515 761 677 1148 245 2120
Fragments arranged by size:
2120 1515 1148 761 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5914
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6466
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6466
NotI GC'GGCC_GC
Cuts at: 3913 3913
Size: 6466
NspI r_CATG'y
Cuts at: 1132 1229 2439 2788 3725 3824 5369 1132
Size: 97 1210 349 937 99 1545 2229
Fragments arranged by size:
2229 1545 1210 937 349 99 97
PasI CC'CwG_GG
Cuts at: 1348 3641 1348
Size: 2293 4173
PciI A'CATG_T
Cuts at: 2784 3721 5365 2784
Size: 937 1644 3885
Fragments arranged by size:
3885 1644 937
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6466
PfoI T'CCnGG_A
Cuts at: 3345 3432 3345
Size: 87 6379
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 4535 4567 6048 6080 2930
Size: 32 1573 32 1481 32 3316
Fragments arranged by size:
3316 1573 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 6466
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6466
PsiI TTA'TAA
Cuts at: 4036 4036
Size: 6466
PstI C_TGCA'G
Cuts at: 830 3120 3734 3818 830
Size: 2290 614 84 3478
Fragments arranged by size:
3478 2290 614 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
PvuII CAG'CTG
Cuts at: 3121 3121
Size: 6466
SacI G_AGCT'C
Cuts at: 2158 2158
Size: 6466
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SbfI CC_TGCA'GG
Cuts at: 3818 3818
Size: 6466
ScaI AGT'ACT
Cuts at: 4714 6426 4714
Size: 1712 4754
SfcI C'TryA_G
Cuts at: 826 1045 3116 3382 3730 3814 4949 5630 5821
Size: 219 2071 266 348 84 1135 681 191
Cuts at: 5821 826
Size: 1471
Fragments arranged by size:
2071 1471 1135 681 348 266 219 191
84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6466
SmaI CCC'GGG
Cuts at: 1872 2522 2816 3793 1872
Size: 650 294 977 4545
Fragments arranged by size:
4545 977 650 294
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 3596 4570 5471 5733 820
Size: 197 926 372 1281 974 901 262 1553
Fragments arranged by size:
1553 1281 974 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 3824 1132
Size: 2692 3774
SspI AAT'ATT
Cuts at: 5 52 4390 5
Size: 47 4338 2081
Fragments arranged by size:
4338 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6466
StyI C'CwwG_G
Cuts at: 513 1065 3093 3905 513
Size: 552 2028 812 3074
Fragments arranged by size:
3074 2028 812 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2206 4493 4510 4663 4848 5293 325
Size: 1255 626 2287 17 153 185 445 1498
Fragments arranged by size:
2287 1498 1255 626 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3357 4712 6424
Size: 274 80 33 51 1443 1380 1355 1712
Cuts at: 6424 96
Size: 138
Fragments arranged by size:
1712 1443 1380 1355 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6426 6458 6426
Size: 32 6434
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6466
XbaI T'CTAG_A
Cuts at: 2851 3802 2851
Size: 951 5515
XhoI C'TCGA_G
Cuts at: 1943 2315 1943
Size: 372 6094
XmaI C'CCGG_G
Cuts at: 1870 2520 2814 3791 1870
Size: 650 294 977 4545
Fragments arranged by size:
4545 977 650 294
XmnI GAAnn'nnTTC
Cuts at: 2165 3258 4595 2165
Size: 1093 1337 4036
Fragments arranged by size:
4036 1337 1093
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4274 276
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI