Restriction map for
pFN21AB6128
(Circular) (Six-base) MAPSORT of: pFN21AB6128.seq Check: 1039 from: 1 to: 6586
pFN21AB6128 6586 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2354 2354
Size: 6586
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4396 278
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3929 1937
Size: 1992 4594
Acc65I G'GTAC_C
Cuts at: 3907 3907
Size: 6586
AclI AA'CG_TT
Cuts at: 1563 4713 5086 1563
Size: 3150 373 3063
Fragments arranged by size:
3150 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3179 3417 3551 4649 6033 6301 1632
Size: 1547 238 134 1098 1384 268 1917
Fragments arranged by size:
1917 1547 1384 1098 268 238 134
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6389
AflIII A'CryG_T
Cuts at: 1203 5485 6462 1203
Size: 4282 977 1327
Fragments arranged by size:
4282 1327 977
AgeI A'CCGG_T
Cuts at: 5417 5417
Size: 6586
AhdI GACnn_n'nnGTC
Cuts at: 5315 5315
Size: 6586
AleI CACnn'nnGTG
Cuts at: 1166 2936 3378 1166
Size: 1770 442 4374
Fragments arranged by size:
4374 1770 442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3761 1184
Size: 2577 4009
AlwNI CAG_nnn'CTG
Cuts at: 2746 3104 3151 4329 5901 2746
Size: 358 47 1178 1572 3431
Fragments arranged by size:
3431 1572 1178 358 47
ApaI G_GGCC'C
Cuts at: 2800 2800
Size: 6586
ApaLI G'TGCA_C
Cuts at: 4643 5799 4643
Size: 1156 5430
ApoI r'AATT_y
Cuts at: 1493 2403 3895 4121 4273 6382 1493
Size: 910 1492 226 152 2109 1697
Fragments arranged by size:
2109 1697 1492 910 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5140 160
Size: 872 4108 1606
Fragments arranged by size:
4108 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
AvaI C'yCGr_G
Cuts at: 1870 1943 2811 3005 3764 3911 1870
Size: 73 868 194 759 147 4545
Fragments arranged by size:
4545 868 759 194 147 73
AvrII C'CTAG_G
Cuts at: 3865 3865
Size: 6586
BamHI G'GATC_C
Cuts at: 3916 4283 3916
Size: 367 6219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2590 3737 3907 5362 618
Size: 325 217 627 803 1147 170 1455 1842
Fragments arranged by size:
1842 1455 1147 803 627 325 217 170
BanII G_rGCy'C
Cuts at: 1391 2156 2477 2734 2800 2876 2919 3006 3841
Size: 765 321 257 66 76 43 87 835
Cuts at: 3841 3905 1391
Size: 64 4072
Fragments arranged by size:
4072 835 765 321 257 87 76 66
64 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3077 3803 928
Size: 971 1178 726 3711
Fragments arranged by size:
3711 1178 971 726
BbvCI CC'TCA_GC
Cuts at: 2162 2595 3803 2162
Size: 433 1208 4945
Fragments arranged by size:
4945 1208 433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2494 2528 2548 2582 2615 2649 3176
Size: 34 557 34 20 34 33 34 527
Cuts at: 3176 3210 4777 4811 1903
Size: 34 1567 34 3678
Fragments arranged by size:
3678 1567 557 527 34 34 34 34
34 34 33 20
BciVI GTATCCnnnnn_n'
Cuts at: 4478 5688 4478
Size: 1210 5376
BclI T'GATC_A
Cuts at: 1548 2136 1548
Size: 588 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2054 3867
Size: 107 122 71 631 605 377 5 1813
Cuts at: 3867 5197 136
Size: 1330 1525
Fragments arranged by size:
1813 1525 1330 631 605 377 122 107
71 5
BglII A'GATC_T
Cuts at: 3543 6581 3543
Size: 3038 3548
BlpI GC'TnA_GC
Cuts at: 2319 2733 4003 2319
Size: 414 1270 4902
Fragments arranged by size:
4902 1270 414
Bme1580I G_kGCm'C
Cuts at: 1790 2800 3238 4647 5803 1790
Size: 1010 438 1409 1156 2573
Fragments arranged by size:
2573 1409 1156 1010 438
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6586
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2274 2695 4317
Size: 416 53 83 399 24 857 421 1622
Cuts at: 4317 5275 442
Size: 958 1753
Fragments arranged by size:
1753 1622 958 857 421 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2235 2235
Size: 6586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2049 2453 2468 2933 3467 3518 3833 5246
Size: 810 404 15 465 534 51 315 1413
Cuts at: 5246 1239
Size: 2579
Fragments arranged by size:
2579 1413 810 534 465 404 315 51
15
Bpu10I CC'TnA_GC
Cuts at: 2162 2220 2595 2991 3803 2162
Size: 58 375 396 812 4945
Fragments arranged by size:
4945 812 396 375 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4711 5576 5874 4711
Size: 865 298 5423
Fragments arranged by size:
5423 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2292 3753 5249 882
Size: 617 793 1461 1496 2219
Fragments arranged by size:
2219 1496 1461 793 617
BsaAI yAC'GTr
Cuts at: 493 1204 2982 4377 493
Size: 711 1778 1395 2702
Fragments arranged by size:
2702 1778 1395 711
BsaBI GATnn'nnATC
Cuts at: 6580 6580
Size: 6586
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3081 4393 4775 5410
Size: 53 83 186 936 1548 1312 382 635
Cuts at: 5410 275
Size: 1451
Fragments arranged by size:
1548 1451 1312 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2694 3516 3603 5019 5417 5691 5838 6249 2694
Size: 822 87 1416 398 274 147 411 3031
Fragments arranged by size:
3031 1416 822 411 398 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2848 2878 2993 3023 1178
Size: 30 165 30 1445 30 115 30 4741
Fragments arranged by size:
4741 1445 165 115 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2372 2612 3065 3278 3431 3782 1186
Size: 838 348 240 453 213 153 351 3990
Fragments arranged by size:
3990 838 453 351 348 240 213 153
BseYI C'CCAG_C
Cuts at: 2781 2846 3237 3486 3787 5789 6285 2781
Size: 65 391 249 301 2002 496 3082
Fragments arranged by size:
3082 2002 496 391 301 249 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 3113 3356 3677 1163
Size: 573 357 1020 243 321 4072
Fragments arranged by size:
4072 1020 573 357 321 243
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4036 4797 5825 1540
Size: 275 186 2035 761 1028 2301
Fragments arranged by size:
2301 2035 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2866 3006 4647 4732 5803 2866
Size: 140 1641 85 1071 3649
Fragments arranged by size:
3649 1641 1071 140 85
BsmI GAATG_Cn'
Cuts at: 4097 4190 4097
Size: 93 6493
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2477 2734 2800 2866 2876 2919
Size: 399 366 321 257 66 66 10 43
Cuts at: 2919 3006 3238 3841 3905 4647 4732 5803 1391
Size: 87 232 603 64 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 366 321 257
232 87 85 66 66 64 43 10
BspEI T'CCGG_A
Cuts at: 3516 6249 3516
Size: 2733 3853
BspHI T'CATG_A
Cuts at: 4475 6205 4475
Size: 1730 4856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 2354 3941 844
Size: 1466 44 1587 3489
Fragments arranged by size:
3489 1587 1466 44
BsrBI CCG'CTC
Cuts at: 1394 1823 4473 1394
Size: 429 2650 3507
Fragments arranged by size:
3507 2650 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5081 5255 65
Size: 1183 3833 174 1396
Fragments arranged by size:
3833 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2667 3111 5230 5417 1700
Size: 110 238 619 444 2119 187 2869
Fragments arranged by size:
2869 2119 619 444 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6586
BssHII G'CGCG_C
Cuts at: 1925 2083 1925
Size: 158 6428
BssSI C'ACGA_G
Cuts at: 3695 4646 5658 3695
Size: 951 1012 4623
Fragments arranged by size:
4623 1012 951
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 2746 3151 794
Size: 1286 666 405 4229
Fragments arranged by size:
4229 1286 666 405
BstBI TT'CG_AA
Cuts at: 5390 5390
Size: 6586
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6586
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 2215 2509 2101
Size: 114 294 6178
Fragments arranged by size:
6178 294 114
BstYI r'GATC_y
Cuts at: 1972 3360 3543 3819 3916 4283 4668 4685 6137
Size: 1388 183 276 97 367 385 17 1452
Cuts at: 6137 6223 6235 6581 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1388 385 367 346 276 183
97 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2868 2868
Size: 6586
BtgI C'CryG_G
Cuts at: 513 1065 2004 2543 513
Size: 552 939 539 4556
Fragments arranged by size:
4556 939 552 539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5847
Fragments arranged by size:
5847 683 56
BtsI GCAGTG_nn'
Cuts at: 2420 4102 4896 4916 2420
Size: 1682 794 20 4090
Fragments arranged by size:
4090 1682 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6586
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6551
DraI TTT'AAA
Cuts at: 3890 3890
Size: 6586
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5593 809
Size: 507 4277 1802
Fragments arranged by size:
4277 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2669 4033
Size: 54 1425 50 275 18 375 464 1364
Cuts at: 4033 4922 6551 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1364 889 464 375 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4033 1537
Size: 275 2221 4090
Fragments arranged by size:
4090 2221 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2315 3155 3393 3525 4516 1353
Size: 962 840 238 132 991 3423
Fragments arranged by size:
3423 991 962 840 238 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 2189 3833 5173 5547 5693
Size: 1064 138 441 159 1644 1340 374 146
Cuts at: 5693 387
Size: 1280
Fragments arranged by size:
1644 1340 1280 1064 441 374 159 146
138
EcoICRI GAG'CTC
Cuts at: 3004 3004
Size: 6586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2453 2468 2933 3179 3417 3467
Size: 393 417 404 15 465 246 238 50
Cuts at: 3467 3518 3551 3833 4649 5246 6033 6301 1239
Size: 51 33 282 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 597 465 417 404 393
282 268 246 238 51 50 33 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2219 3845 2219
Size: 1626 4960
EcoO109I rG'GnC_Cy
Cuts at: 2431 2785 2796 2797 2815 3148 3280 2431
Size: 354 11 1 18 333 132 5737
Fragments arranged by size:
5737 354 333 132 18 11 1
EcoRI G'AATT_C
Cuts at: 3895 4273 3895
Size: 378 6208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 6586
FspI TGC'GCA
Cuts at: 5092 5092
Size: 6586
HaeII r_GCGC'y
Cuts at: 1678 5733 1678
Size: 4055 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2752 2784 2848 2880 2993
Size: 32 135 32 1180 32 64 32 113
Cuts at: 2993 3025 3323 3355 5227 5259 5301 5333 1373
Size: 32 298 32 1872 32 42 32 2626
Fragments arranged by size:
2626 1872 1180 298 135 113 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3930 4176 669
Size: 988 281 1992 246 3079
Fragments arranged by size:
3079 1992 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6586
HpaI GTT'AAC
Cuts at: 4176 4176
Size: 6586
KpnI G_GTAC'C
Cuts at: 3911 3911
Size: 6586
MfeI C'AATT_G
Cuts at: 4185 4185
Size: 6586
MluI A'CGCG_T
Cuts at: 6462 6462
Size: 6586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2584 5700 5884 1305
Size: 1279 3116 184 2007
Fragments arranged by size:
3116 2007 1279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2207 10
Size: 54 1425 343 375 4389
Fragments arranged by size:
4389 1425 375 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2936 3378 3743 4544 4903 5062 518
Size: 648 1770 442 365 801 359 159 2042
Fragments arranged by size:
2042 1770 801 648 442 365 359 159
MspA1I CmG'CkG
Cuts at: 1606 2124 2416 2737 2743 3025 3241 3433 4002
Size: 518 292 321 6 282 216 192 569
Cuts at: 4002 4679 5827 6072 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 569 518 321 292 282
245 216 192 6
NaeI GCC'GGC
Cuts at: 2050 3113 2050
Size: 1063 5523
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5095
Fragments arranged by size:
5095 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6586
NgoMIV G'CCGG_C
Cuts at: 2048 3111 2048
Size: 1063 5523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6586
NotI GC'GGCC_GC
Cuts at: 4033 4033
Size: 6586
NspI r_CATG'y
Cuts at: 1132 1229 3944 5489 1132
Size: 97 2715 1545 2229
Fragments arranged by size:
2715 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3076 1348
Size: 1728 4858
PciI A'CATG_T
Cuts at: 5485 5485
Size: 6586
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6586
PfoI T'CCnGG_A
Cuts at: 3495 3822 3876 3495
Size: 327 54 6205
Fragments arranged by size:
6205 327 54
PmeI GTTT'AAAC
Cuts at: 3890 3890
Size: 6586
PmlI CAC'GTG
Cuts at: 2982 2982
Size: 6586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2988 3020 3617 3649 4655 4687 6168 6200 2988
Size: 32 597 32 1006 32 1481 32 3374
Fragments arranged by size:
3374 1481 1006 597 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2815 3280 2431
Size: 384 465 5737
Fragments arranged by size:
5737 465 384
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6586
PsiI TTA'TAA
Cuts at: 4156 4156
Size: 6586
PspOMI G'GGCC_C
Cuts at: 2796 2796
Size: 6586
PspXI vC'TCGA_Gb
Cuts at: 3005 3764 3005
Size: 759 5827
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2258 2290 2258
Size: 32 6554
PstI C_TGCA'G
Cuts at: 830 2365 2463 2643 2751 2835 3216 3321 3707
Size: 1535 98 180 108 84 381 105 386
Cuts at: 3707 3938 830
Size: 231 3478
Fragments arranged by size:
3478 1535 386 381 231 180 108 105
98 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6586
PvuII CAG'CTG
Cuts at: 2416 3025 3433 2416
Size: 609 408 5569
Fragments arranged by size:
5569 609 408
RsrII CG'GwC_CG
Cuts at: 2017 2017
Size: 6586
SacI G_AGCT'C
Cuts at: 3006 3006
Size: 6586
SalI G'TCGA_C
Cuts at: 1936 3928 1936
Size: 1992 4594
SanDI GG'GwC_CC
Cuts at: 2431 3280 2431
Size: 849 5737
SapI GCTCTTCn'nnn_
Cuts at: 3155 3155
Size: 6586
SbfI CC_TGCA'GG
Cuts at: 2365 3938 2365
Size: 1573 5013
ScaI AGT'ACT
Cuts at: 4834 6546 4834
Size: 1712 4874
SfcI C'TryA_G
Cuts at: 826 1045 2361 2459 2639 2747 2831 3212 3317
Size: 219 1316 98 180 108 84 381 105
Cuts at: 3317 3703 3934 5069 5750 5941 826
Size: 386 231 1135 681 191 1471
Fragments arranged by size:
1471 1316 1135 681 386 381 231 219
191 180 108 105 98 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 3867 3867
Size: 6586
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6586
SmaI CCC'GGG
Cuts at: 1872 2813 3913 1872
Size: 941 1100 4545
Fragments arranged by size:
4545 1100 941
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3005 3764 4690 5591 5853 820
Size: 197 926 1062 759 926 901 262 1553
Fragments arranged by size:
1553 1062 926 926 901 759 262 197
SnaBI TAC'GTA
Cuts at: 493 4377 493
Size: 3884 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6586
SphI G_CATG'C
Cuts at: 1132 3944 1132
Size: 2812 3774
SspI AAT'ATT
Cuts at: 5 52 4510 5
Size: 47 4458 2081
Fragments arranged by size:
4458 2081 47
StuI AGG'CCT
Cuts at: 1517 2225 2837 3671 1517
Size: 708 612 834 4432
Fragments arranged by size:
4432 834 708 612
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2426 3865 4025 513
Size: 552 939 243 179 1439 160 3074
Fragments arranged by size:
3074 1439 939 552 243 179 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2034 3500 4613 4630 4783 4968 5413
Size: 1255 454 1466 1113 17 153 185 445
Cuts at: 5413 325
Size: 1498
Fragments arranged by size:
1498 1466 1255 1113 454 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3128 4832 6544
Size: 274 80 33 51 1443 1151 1704 1712
Cuts at: 6544 96
Size: 138
Fragments arranged by size:
1712 1704 1443 1151 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2044 2076 2535 2567 2684 2716 6546 6578 2044
Size: 32 459 32 117 32 3830 32 2052
Fragments arranged by size:
3830 2052 459 117 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2687 1316
Size: 1371 5215
XbaI T'CTAG_A
Cuts at: 3922 3922
Size: 6586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3854 3854
Size: 6586
XhoI C'TCGA_G
Cuts at: 1943 3005 3764 1943
Size: 1062 759 4765
Fragments arranged by size:
4765 1062 759
XmaI C'CCGG_G
Cuts at: 1870 2811 3911 1870
Size: 941 1100 4545
Fragments arranged by size:
4545 1100 941
XmnI GAAnn'nnTTC
Cuts at: 2492 4715 2492
Size: 2223 4363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4394 276
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI
NsiI PacI SacII SexAI SfoI SrfI SwaI