Restriction map for
pFN21AB6127
(Circular) (Six-base) MAPSORT of: pFN21AB6127.seq Check: 3794 from: 1 to: 6466
pFN21AB6127 6466 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3183 4276 278
Size: 53 83 186 936 1647 1093 2468
Fragments arranged by size:
2468 1647 1093 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3809 1937
Size: 1872 4594
Acc65I G'GTAC_C
Cuts at: 3787 3787
Size: 6466
AclI AA'CG_TT
Cuts at: 1563 4593 4966 1563
Size: 3030 373 3063
Fragments arranged by size:
3063 3030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3599 4529 5913 6181 1632
Size: 1967 930 1384 268 1917
Fragments arranged by size:
1967 1917 1384 930 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6269
AflIII A'CryG_T
Cuts at: 1203 2772 3129 5365 6342 1203
Size: 1569 357 2236 977 1327
Fragments arranged by size:
2236 1569 1327 977 357
AgeI A'CCGG_T
Cuts at: 5297 5297
Size: 6466
AhdI GACnn_n'nnGTC
Cuts at: 2773 5195 2773
Size: 2422 4044
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6466
AlwNI CAG_nnn'CTG
Cuts at: 2629 2926 3238 4209 5781 2629
Size: 297 312 971 1572 3314
Fragments arranged by size:
3314 1572 971 312 297
ApaI G_GGCC'C
Cuts at: 2104 3525 2104
Size: 1421 5045
ApaLI G'TGCA_C
Cuts at: 4523 5679 4523
Size: 1156 5310
ApoI r'AATT_y
Cuts at: 1493 3775 4001 4153 6262 1493
Size: 2282 226 152 2109 1697
Fragments arranged by size:
2282 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2472 3062 3791 1870
Size: 73 154 375 590 729 4545
Fragments arranged by size:
4545 729 590 375 154 73
BamHI G'GATC_C
Cuts at: 3796 4163 3796
Size: 367 6099
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2840 3058 3161 3787
Size: 325 217 627 383 670 218 103 626
Cuts at: 3787 5242 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 670 627 626 383 325 218
217 103
BanII G_rGCy'C
Cuts at: 1391 2104 2262 3372 3465 3525 3785 1391
Size: 713 158 1110 93 60 260 4072
Fragments arranged by size:
4072 1110 713 260 158 93 60
BbeI G_GCGC'C
Cuts at: 2174 3062 3165 2174
Size: 888 103 5475
Fragments arranged by size:
5475 888 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 3090 928
Size: 971 332 859 4304
Fragments arranged by size:
4304 971 859 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4657 4691 1903
Size: 34 2720 34 3678
Fragments arranged by size:
3678 2720 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4358 5568 4358
Size: 1210 5256
BclI T'GATC_A
Cuts at: 1548 2232 1548
Size: 684 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2313 2964 3390
Size: 107 122 71 631 605 641 651 426
Cuts at: 3390 5077 136
Size: 1687 1525
Fragments arranged by size:
1687 1525 651 641 631 605 426 122
107 71
BglII A'GATC_T
Cuts at: 6461 6461
Size: 6466
BlpI GC'TnA_GC
Cuts at: 3026 3883 3026
Size: 857 5609
Bme1580I G_kGCm'C
Cuts at: 1790 2104 3525 4527 5683 1790
Size: 314 1421 1002 1156 2573
Fragments arranged by size:
2573 1421 1156 1002 314
BmgBI CAC'GTC
Cuts at: 1318 3043 1318
Size: 1725 4741
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3616 4197 5155
Size: 416 53 83 399 24 2199 581 958
Cuts at: 5155 442
Size: 1753
Fragments arranged by size:
2199 1753 958 581 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2047 2633 3035 3409 5126 1239
Size: 808 586 402 374 1717 2579
Fragments arranged by size:
2579 1717 808 586 402 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2740 3320 4591 5456 5754 2740
Size: 580 1271 865 298 3452
Fragments arranged by size:
3452 1271 865 580 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5129 882
Size: 617 3630 2219
Fragments arranged by size:
3630 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3550 4257 493
Size: 711 2346 707 2702
Fragments arranged by size:
2702 2346 711 707
BsaBI GATnn'nnATC
Cuts at: 6460 6460
Size: 6466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2171 2847 2967 3059
Size: 53 83 186 936 638 676 120 92
Cuts at: 3059 3162 3180 3264 4273 4655 5290 275
Size: 103 18 84 1009 382 635 1451
Fragments arranged by size:
1451 1009 936 676 638 635 382 186
120 103 92 84 83 53 18
BsaWI w'CCGG_w
Cuts at: 4899 5297 5571 5718 6129 4899
Size: 398 274 147 411 5236
Fragments arranged by size:
5236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2062 2092 2634 2664 2876
Size: 30 165 30 659 30 542 30 212
Cuts at: 2876 2906 1178
Size: 30 4738
Fragments arranged by size:
4738 659 542 212 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2655 2825 1186
Size: 1469 170 4827
Fragments arranged by size:
4827 1469 170
BseYI C'CCAG_C
Cuts at: 2163 3464 3513 5669 6165 2163
Size: 1301 49 2156 496 2464
Fragments arranged by size:
2464 2156 1301 496 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2040 2058 2270 2422 2555 2570 2867
Size: 573 304 18 212 152 133 15 297
Cuts at: 2867 2939 2963 3494 3581 1163
Size: 72 24 531 87 4048
Fragments arranged by size:
4048 573 531 304 297 212 152 133
87 72 24 18 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2991 3453 3916 4677 5705 1540
Size: 275 186 990 462 463 761 1028 2301
Fragments arranged by size:
2301 1028 990 761 463 462 275 186
BsiHKAI G_wGCw'C
Cuts at: 4527 4612 5683 4527
Size: 85 1071 5310
Fragments arranged by size:
5310 1071 85
BsmI GAATG_Cn'
Cuts at: 2668 3977 4070 2668
Size: 1309 93 5064
Fragments arranged by size:
5064 1309 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2262 3372 3465 3525 3785 4527
Size: 399 314 158 1110 93 60 260 742
Cuts at: 4527 4612 5683 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1110 1071 742 399 314 260 158
93 85 60
BspEI T'CCGG_A
Cuts at: 6129 6129
Size: 6466
BspHI T'CATG_A
Cuts at: 4355 6085 4355
Size: 1730 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3420 3821 844
Size: 2576 401 3489
Fragments arranged by size:
3489 2576 401
BsrBI CCG'CTC
Cuts at: 1394 1823 2485 2582 2637 3721 4353 1394
Size: 429 662 97 55 1084 632 3507
Fragments arranged by size:
3507 1084 662 632 429 97 55
BsrDI GCAATG_nn'
Cuts at: 65 1248 2611 4961 5135 65
Size: 1183 1363 2350 174 1396
Fragments arranged by size:
2350 1396 1363 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 2932 3087 3409 5110 5297 1700
Size: 110 282 840 155 322 1701 187 2869
Fragments arranged by size:
2869 1701 840 322 282 187 155 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6466
BssHII G'CGCG_C
Cuts at: 1925 3030 3032 3067 3069 3071 1925
Size: 1105 2 35 2 2 5320
Fragments arranged by size:
5320 1105 35 2 2 2
BssSI C'ACGA_G
Cuts at: 2288 2588 3197 4526 5538 2288
Size: 300 609 1329 1012 3216
Fragments arranged by size:
3216 1329 1012 609 300
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2926 3238 794
Size: 2132 312 4022
Fragments arranged by size:
4022 2132 312
BstBI TT'CG_AA
Cuts at: 3673 5270 3673
Size: 1597 4869
BstEII G'GTnAC_C
Cuts at: 1184 2935 1184
Size: 1751 4715
BstYI r'GATC_y
Cuts at: 1972 2625 3796 4163 4548 4565 6017 6103 6115
Size: 653 1171 367 385 17 1452 86 12
Cuts at: 6115 6461 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1171 653 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2569 3211 3247 3447 513
Size: 552 1504 642 36 200 3532
Fragments arranged by size:
3532 1504 642 552 200 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2223 2872 487
Size: 683 56 997 649 4081
Fragments arranged by size:
4081 997 683 649 56
BtsI GCAGTG_nn'
Cuts at: 2070 2429 3982 4776 4796 2070
Size: 359 1553 794 20 3740
Fragments arranged by size:
3740 1553 794 359 20
ClaI AT'CG_AT
Cuts at: 1554 3531 1554
Size: 1977 4489
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6431
DraI TTT'AAA
Cuts at: 3770 3770
Size: 6466
DraIII CAC_nnn'GTG
Cuts at: 2840 2840
Size: 6466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2615 3611 5473 809
Size: 507 1299 996 1862 1802
Fragments arranged by size:
1862 1802 1299 996 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2305 2691 2988
Size: 54 1425 50 275 18 475 386 297
Cuts at: 2988 3201 3450 3913 4802 6431 8
Size: 213 249 463 889 1629 43
Fragments arranged by size:
1629 1425 889 475 463 386 297 275
249 213 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2988 3450 3913 1537
Size: 275 1176 462 463 4090
Fragments arranged by size:
4090 1176 463 462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2208 4396 1353
Size: 855 2188 3423
Fragments arranged by size:
3423 2188 855
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3197 5053 5427 5573 387
Size: 1064 138 1608 1856 374 146 1280
Fragments arranged by size:
1856 1608 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2633 3035 3409 3599 4529 5126
Size: 393 415 586 402 374 190 930 597
Cuts at: 5126 5913 6181 1239
Size: 787 268 1524
Fragments arranged by size:
1524 930 787 597 586 415 402 393
374 268 190
EcoNI CCTnn'n_nnAGG
Cuts at: 2372 2786 3427 2372
Size: 414 641 5411
Fragments arranged by size:
5411 641 414
EcoO109I rG'GnC_Cy
Cuts at: 2101 3521 2101
Size: 1420 5046
EcoRI G'AATT_C
Cuts at: 3775 4153 3775
Size: 378 6088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 6466
FspI TGC'GCA
Cuts at: 3224 4972 3224
Size: 1748 4718
HaeII r_GCGC'y
Cuts at: 1678 2174 3023 3062 3165 5613 1678
Size: 496 849 39 103 2448 2531
Fragments arranged by size:
2531 2448 849 496 103 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 2759 2791 3476
Size: 32 135 32 1010 32 145 32 685
Cuts at: 3476 3508 5107 5139 5181 5213 1373
Size: 32 1599 32 42 32 2626
Fragments arranged by size:
2626 1599 1010 685 145 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3121 3810 4056 669
Size: 988 281 1183 689 246 3079
Fragments arranged by size:
3079 1183 988 689 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6466
HpaI GTT'AAC
Cuts at: 4056 4056
Size: 6466
KasI G'GCGC_C
Cuts at: 2170 3058 3161 2170
Size: 888 103 5475
Fragments arranged by size:
5475 888 103
KpnI G_GTAC'C
Cuts at: 3791 3791
Size: 6466
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 2772 6342 2772
Size: 3570 2896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 2691 5580 5764 1305
Size: 1182 204 2889 184 2007
Fragments arranged by size:
2889 2007 1182 204 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2307 3203 10
Size: 54 1425 343 475 896 3273
Fragments arranged by size:
3273 1425 896 475 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3305 4424 4783 4942 518
Size: 648 2139 1119 359 159 2042
Fragments arranged by size:
2139 2042 1119 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2284 2562 2571 2881 2977 3055 3235
Size: 561 117 278 9 310 96 78 180
Cuts at: 3235 3449 3513 3882 4559 5707 5952 1606
Size: 214 64 369 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 561 369 310 278 245
214 180 117 96 78 64 9
NaeI GCC'GGC
Cuts at: 2094 3411 2094
Size: 1317 5149
NarI GG'CG_CC
Cuts at: 2171 3059 3162 2171
Size: 888 103 5475
Fragments arranged by size:
5475 888 103
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5914
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6466
NgoMIV G'CCGG_C
Cuts at: 2092 3409 2092
Size: 1317 5149
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6466
NotI GC'GGCC_GC
Cuts at: 3913 3913
Size: 6466
NspI r_CATG'y
Cuts at: 1132 1229 3824 5369 1132
Size: 97 2595 1545 2229
Fragments arranged by size:
2595 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2345 1348
Size: 997 5469
PciI A'CATG_T
Cuts at: 5365 5365
Size: 6466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6466
PfoI T'CCnGG_A
Cuts at: 3540 3540
Size: 6466
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 6048 6080 4535
Size: 32 1481 32 4921
Fragments arranged by size:
4921 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6466
PsiI TTA'TAA
Cuts at: 4036 4036
Size: 6466
PspOMI G'GGCC_C
Cuts at: 2100 3521 2100
Size: 1421 5045
PstI C_TGCA'G
Cuts at: 830 2334 2373 3818 830
Size: 1504 39 1445 3478
Fragments arranged by size:
3478 1504 1445 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
PvuII CAG'CTG
Cuts at: 2167 3235 2167
Size: 1068 5398
SacII CC_GC'GG
Cuts at: 2572 3450 2572
Size: 878 5588
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SbfI CC_TGCA'GG
Cuts at: 2373 3818 2373
Size: 1445 5021
ScaI AGT'ACT
Cuts at: 2030 4714 6426 2030
Size: 2684 1712 2070
Fragments arranged by size:
2684 2070 1712
SfcI C'TryA_G
Cuts at: 826 1045 2330 2369 2908 3814 4949 5630 5821
Size: 219 1285 39 539 906 1135 681 191
Cuts at: 5821 826
Size: 1471
Fragments arranged by size:
1471 1285 1135 906 681 539 219 191
39
SfoI GGC'GCC
Cuts at: 2172 3060 3163 2172
Size: 888 103 5475
Fragments arranged by size:
5475 888 103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6466
SmaI CCC'GGG
Cuts at: 1872 2099 3793 1872
Size: 227 1694 4545
Fragments arranged by size:
4545 1694 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2719 3335 4570 5471 5733 820
Size: 197 926 776 616 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 776 616 262 197
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 3824 1132
Size: 2692 3774
SrfI GCCC'GGGC
Cuts at: 2099 2099
Size: 6466
SspI AAT'ATT
Cuts at: 5 52 4390 5
Size: 47 4338 2081
Fragments arranged by size:
4338 2081 47
StuI AGG'CCT
Cuts at: 1517 2219 2717 2792 1517
Size: 702 498 75 5191
Fragments arranged by size:
5191 702 498 75
StyI C'CwwG_G
Cuts at: 513 1065 2119 2646 3905 513
Size: 552 1054 527 1259 3074
Fragments arranged by size:
3074 1259 1054 552 527
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 4493 4510 4663 4848 5293 325
Size: 1255 2196 717 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2028 3105 4712
Size: 274 80 33 51 1443 51 1077 1607
Cuts at: 4712 6424 96
Size: 1712 138
Fragments arranged by size:
1712 1607 1443 1077 274 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 2634 2666 2876 2908 6426 6458 2062
Size: 32 540 32 210 32 3518 32 2070
Fragments arranged by size:
3518 2070 540 210 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6466
XbaI T'CTAG_A
Cuts at: 3802 3802
Size: 6466
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6466
XmaI C'CCGG_G
Cuts at: 1870 2097 3791 1870
Size: 227 1694 4545
Fragments arranged by size:
4545 1694 227
XmnI GAAnn'nnTTC
Cuts at: 4595 4595
Size: 6466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3181 4274 276
Size: 53 83 186 936 1647 1093 2468
Fragments arranged by size:
2468 1647 1093 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SwaI XcmI