Restriction map for
pFN21AB6124
(Circular) (Six-base) MAPSORT of: pFN21AB6124.seq Check: 7606 from: 1 to: 6583
pFN21AB6124 6583 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4393 278
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3063 3926 1937
Size: 1126 863 4594
Fragments arranged by size:
4594 1126 863
Acc65I G'GTAC_C
Cuts at: 2090 2299 3904 2090
Size: 209 1605 4769
Fragments arranged by size:
4769 1605 209
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2757 4646 6030 6298 1632
Size: 1125 1889 1384 268 1917
Fragments arranged by size:
1917 1889 1384 1125 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 5482 6459 1203
Size: 4279 977 1327
Fragments arranged by size:
4279 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6583
AlwNI CAG_nnn'CTG
Cuts at: 2528 3836 4326 5898 2528
Size: 1308 490 1572 3213
Fragments arranged by size:
3213 1572 1308 490
ApaI G_GGCC'C
Cuts at: 2063 2063
Size: 6583
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 3218 3892 4118 4270 6379 1493
Size: 1725 674 226 152 2109 1697
Fragments arranged by size:
2109 1725 1697 674 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2649 3468 3908 1870
Size: 73 113 593 819 440 4545
Fragments arranged by size:
4545 819 593 440 113 73
BamHI G'GATC_C
Cuts at: 3913 4280 3913
Size: 367 6216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2299 2384 3904 5359
Size: 325 217 627 303 209 85 1520 1455
Cuts at: 5359 618
Size: 1842
Fragments arranged by size:
1842 1520 1455 627 325 303 217 209
85
BanII G_rGCy'C
Cuts at: 1391 2063 3902 1391
Size: 672 1839 4072
Fragments arranged by size:
4072 1839 672
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2524 2793 2910 928
Size: 971 625 269 117 4601
Fragments arranged by size:
4601 971 625 269 117
BbvCI CC'TCA_GC
Cuts at: 2936 3504 2936
Size: 568 6015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4774 4808 1903
Size: 34 2837 34 3678
Fragments arranged by size:
3678 2837 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4475 5685 4475
Size: 1210 5373
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5194 136
Size: 107 122 71 631 605 3522 1525
Fragments arranged by size:
3522 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 4000 4000
Size: 6583
Bme1580I G_kGCm'C
Cuts at: 1790 2063 4644 5800 1790
Size: 273 2581 1156 2573
Fragments arranged by size:
2581 2573 1156 273
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6583
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3777 3851 4314
Size: 416 53 83 399 24 2360 74 463
Cuts at: 4314 5272 442
Size: 958 1753
Fragments arranged by size:
2360 1753 958 463 416 399 83 74
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2644 3603 2644
Size: 959 5624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2748 3432 3544 3591 3624 5243 1239
Size: 1509 684 112 47 33 1619 2579
Fragments arranged by size:
2579 1619 1509 684 112 47 33
Bpu10I CC'TnA_GC
Cuts at: 2348 2936 3504 3678 2348
Size: 588 568 174 5253
Fragments arranged by size:
5253 588 568 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4708 5573 5871 4708
Size: 865 298 5420
Fragments arranged by size:
5420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5246 882
Size: 617 3747 2219
Fragments arranged by size:
3747 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4374 493
Size: 711 3170 2702
Fragments arranged by size:
3170 2702 711
BsaBI GATnn'nnATC
Cuts at: 6577 6577
Size: 6583
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2254 4390 4772 5407
Size: 53 83 186 936 721 2136 382 635
Cuts at: 5407 275
Size: 1451
Fragments arranged by size:
2136 1451 936 721 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5016 5414 5688 5835 6246 5016
Size: 398 274 147 411 5353
Fragments arranged by size:
5353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6358
Fragments arranged by size:
6358 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2620 2623 1186
Size: 1434 3 5146
Fragments arranged by size:
5146 1434 3
BseYI C'CCAG_C
Cuts at: 3473 5786 6282 3473
Size: 2313 496 3774
Fragments arranged by size:
3774 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2827 1163
Size: 573 1091 4919
Fragments arranged by size:
4919 1091 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4033 4794 5822 1540
Size: 275 186 2032 761 1028 2301
Fragments arranged by size:
2301 2032 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2734 4644 4729 5800 2734
Size: 1910 85 1071 3517
Fragments arranged by size:
3517 1910 1071 85
BsmI GAATG_Cn'
Cuts at: 2965 4094 4187 2965
Size: 1129 93 5361
Fragments arranged by size:
5361 1129 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2734 3902 4644 4729 5800 1391
Size: 399 273 671 1168 742 85 1071 2174
Fragments arranged by size:
2174 1168 1071 742 671 399 273 85
BspEI T'CCGG_A
Cuts at: 6246 6246
Size: 6583
BspHI T'CATG_A
Cuts at: 3273 3813 4472 6202 3273
Size: 540 659 1730 3654
Fragments arranged by size:
3654 1730 659 540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2598 2816 3938 844
Size: 1235 519 218 1122 3489
Fragments arranged by size:
3489 1235 1122 519 218
BsrBI CCG'CTC
Cuts at: 1394 1823 2656 4470 1394
Size: 429 833 1814 3507
Fragments arranged by size:
3507 1814 833 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2322 3807 5078 5252 65
Size: 1183 1074 1485 1271 174 1396
Fragments arranged by size:
1485 1396 1271 1183 1074 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5227 5414 1700
Size: 110 3417 187 2869
Fragments arranged by size:
3417 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6583
BssSI C'ACGA_G
Cuts at: 3577 4643 5655 3577
Size: 1066 1012 4505
Fragments arranged by size:
4505 1066 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6583
BstBI TT'CG_AA
Cuts at: 5387 5387
Size: 6583
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6583
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 2890 3340 2101
Size: 789 450 5344
Fragments arranged by size:
5344 789 450
BstYI r'GATC_y
Cuts at: 1972 2538 3913 4280 4665 4682 6134 6220 6232
Size: 566 1375 367 385 17 1452 86 12
Cuts at: 6232 6578 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1375 566 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3872 513
Size: 552 939 1868 3224
Fragments arranged by size:
3224 1868 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3525 487
Size: 683 56 2299 3545
Fragments arranged by size:
3545 2299 683 56
BtsI GCAGTG_nn'
Cuts at: 4099 4893 4913 4099
Size: 794 20 5769
Fragments arranged by size:
5769 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3223 3627 3887 3223
Size: 404 260 5919
Fragments arranged by size:
5919 404 260
DraIII CAC_nnn'GTG
Cuts at: 3120 3120
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2876 3875 4030
Size: 54 1425 50 275 18 1046 999 155
Cuts at: 4030 4919 6548 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1046 999 889 275 155 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4030 1537
Size: 275 2218 4090
Fragments arranged by size:
4090 2218 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3607 4513 1353
Size: 2254 906 3423
Fragments arranged by size:
3423 2254 906
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5170 5544 5690 387
Size: 1064 138 3581 374 146 1280
Fragments arranged by size:
3581 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2748 2757 3432 3544 3591 3624 4646
Size: 393 1116 9 675 112 47 33 1022
Cuts at: 4646 5243 6030 6298 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1116 1022 787 675 597 393 268
112 47 33 9
EcoO109I rG'GnC_Cy
Cuts at: 2060 3352 3432 2060
Size: 1292 80 5211
Fragments arranged by size:
5211 1292 80
EcoRI G'AATT_C
Cuts at: 3892 4270 3892
Size: 378 6205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 6583
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 5730 1678
Size: 4052 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2786 2818 3755
Size: 32 135 32 464 32 718 32 937
Cuts at: 3755 3787 5224 5256 5298 5330 1373
Size: 32 1437 32 42 32 2626
Fragments arranged by size:
2626 1437 937 718 464 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3856 3927 4173 669
Size: 988 281 1918 71 246 3079
Fragments arranged by size:
3079 1918 988 281 246 71
HindIII A'AGCT_T
Cuts at: 748 2836 748
Size: 2088 4495
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KpnI G_GTAC'C
Cuts at: 2094 2303 3908 2094
Size: 209 1605 4769
Fragments arranged by size:
4769 1605 209
MfeI C'AATT_G
Cuts at: 2952 4182 2952
Size: 1230 5353
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5697 5881 1305
Size: 4392 184 2007
Fragments arranged by size:
4392 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2878 10
Size: 54 1425 343 1046 3715
Fragments arranged by size:
3715 1425 1046 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2888 2970 3141 4541 4900 5059 518
Size: 648 1722 82 171 1400 359 159 2042
Fragments arranged by size:
2042 1722 1400 648 359 171 159 82
MspA1I CmG'CkG
Cuts at: 1606 2940 3880 3999 4676 5824 6069 1606
Size: 1334 940 119 677 1148 245 2120
Fragments arranged by size:
2120 1334 1148 940 677 245 119
NcoI C'CATG_G
Cuts at: 513 1065 2004 3872 513
Size: 552 939 1868 3224
Fragments arranged by size:
3224 1868 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6583
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 2310 3403 3941 5486 1132
Size: 97 1081 1093 538 1545 2229
Fragments arranged by size:
2229 1545 1093 1081 538 97
PasI CC'CwG_GG
Cuts at: 1348 2705 1348
Size: 1357 5226
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2020 3391 1108
Size: 912 1371 4300
Fragments arranged by size:
4300 1371 912
PfoI T'CCnGG_A
Cuts at: 2321 3339 3607 2321
Size: 1018 268 5297
Fragments arranged by size:
5297 1018 268
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 6165 6197 4652
Size: 32 1481 32 5038
Fragments arranged by size:
5038 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3352 3432 3352
Size: 80 6503
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PspOMI G'GGCC_C
Cuts at: 2059 2059
Size: 6583
PstI C_TGCA'G
Cuts at: 830 3935 830
Size: 3105 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 2940 2940
Size: 6583
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SanDI GG'GwC_CC
Cuts at: 3352 3352
Size: 6583
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 4831 6543 4831
Size: 1712 4871
SfcI C'TryA_G
Cuts at: 826 1045 2548 2749 3931 5066 5747 5938 826
Size: 219 1503 201 1182 1135 681 191 1471
Fragments arranged by size:
1503 1471 1182 1135 681 219 201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 2058 2651 3470 3910 1872
Size: 186 593 819 440 4545
Fragments arranged by size:
4545 819 593 440 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4687 5588 5850 820
Size: 197 926 2744 901 262 1553
Fragments arranged by size:
2744 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 3403 3941 1132
Size: 2271 538 3774
Fragments arranged by size:
3774 2271 538
SspI AAT'ATT
Cuts at: 5 52 3131 4507 5
Size: 47 3079 1376 2081
Fragments arranged by size:
3079 2081 1376 47
StuI AGG'CCT
Cuts at: 1517 2770 1517
Size: 1253 5330
StyI C'CwwG_G
Cuts at: 513 1065 2004 2203 3281 3288 3766 3872 4022
Size: 552 939 199 1078 7 478 106 150
Cuts at: 4022 513
Size: 3074
Fragments arranged by size:
3074 1078 939 552 478 199 150 106
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2514 2896 3833 4610 4627 4780 4965
Size: 1255 934 382 937 777 17 153 185
Cuts at: 4965 5410 325
Size: 445 1498
Fragments arranged by size:
1498 1255 937 934 777 445 382 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4829 6541 96
Size: 274 80 33 51 1443 2852 1712 138
Fragments arranged by size:
2852 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6543 6575 6543
Size: 32 6551
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6583
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 2056 2649 3468 3908 1870
Size: 186 593 819 440 4545
Fragments arranged by size:
4545 819 593 440 186
XmnI GAAnn'nnTTC
Cuts at: 3132 4712 3132
Size: 1580 5003
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4391 276
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI