Restriction map for
pFN21AB6123
(Circular) (Six-base) MAPSORT of: pFN21AB6123.seq Check: 1879 from: 1 to: 6463
pFN21AB6123 6463 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 2791 3806 1937
Size: 169 685 1015 4594
Fragments arranged by size:
4594 1015 685 169
Acc65I G'GTAC_C
Cuts at: 3784 3784
Size: 6463
AclI AA'CG_TT
Cuts at: 1563 4590 4963 1563
Size: 3027 373 3063
Fragments arranged by size:
3063 3027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2156 2379 2502 2670 4526 5910 6178
Size: 455 69 223 123 168 1856 1384 268
Cuts at: 6178 1632
Size: 1917
Fragments arranged by size:
1917 1856 1384 455 268 223 168 123
69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6266
AflIII A'CryG_T
Cuts at: 1203 5362 6339 1203
Size: 4159 977 1327
Fragments arranged by size:
4159 1327 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 5192 5192
Size: 6463
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3102 3106 1184
Size: 1918 4 4541
Fragments arranged by size:
4541 1918 4
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2108 2140 2108
Size: 32 6431
AlwNI CAG_nnn'CTG
Cuts at: 4206 5778 4206
Size: 1572 4891
ApaLI G'TGCA_C
Cuts at: 2021 2536 4520 5676 2021
Size: 515 1984 1156 2808
Fragments arranged by size:
2808 1984 1156 515
ApoI r'AATT_y
Cuts at: 1493 2385 2701 2728 2885 3772 3998 4150 6259
Size: 892 316 27 157 887 226 152 2109
Cuts at: 6259 1493
Size: 1697
Fragments arranged by size:
2109 1697 892 887 316 226 157 152
27
AseI AT'TA_AT
Cuts at: 160 1032 5017 160
Size: 872 3985 1606
Fragments arranged by size:
3985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 3788 1870
Size: 73 1845 4545
Fragments arranged by size:
4545 1845 73
BamHI G'GATC_C
Cuts at: 3793 4160 3793
Size: 367 6096
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 3784 5239 618
Size: 325 217 627 297 1700 1455 1842
Fragments arranged by size:
1842 1700 1455 627 325 297 217
BanII G_rGCy'C
Cuts at: 1391 2043 2316 3782 1391
Size: 652 273 1466 4072
Fragments arranged by size:
4072 1466 652 273
BbeI G_GCGC'C
Cuts at: 2088 2088
Size: 6463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3491 928
Size: 971 1592 3900
Fragments arranged by size:
3900 1592 971
BbvCI CC'TCA_GC
Cuts at: 3426 3426
Size: 6463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2716 2750 4654 4688 1903
Size: 34 779 34 1904 34 3678
Fragments arranged by size:
3678 1904 779 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4355 5565 3374
Size: 981 1210 4272
Fragments arranged by size:
4272 1210 981
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6463
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2993 3009 5074
Size: 107 122 71 631 605 1321 16 2065
Cuts at: 5074 136
Size: 1525
Fragments arranged by size:
2065 1525 1321 631 605 122 107 71
16
BglII A'GATC_T
Cuts at: 2815 3462 6458 2815
Size: 647 2996 2820
Fragments arranged by size:
2996 2820 647
BlpI GC'TnA_GC
Cuts at: 3880 3880
Size: 6463
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2540 3680 4524 5680 1790
Size: 235 515 1140 844 1156 2573
Fragments arranged by size:
2573 1156 1140 844 515 235
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4194 5152 442
Size: 416 53 83 399 24 2777 958 1753
Fragments arranged by size:
2777 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2385 5123 1239
Size: 1146 2738 2579
Fragments arranged by size:
2738 2579 1146
Bpu10I CC'TnA_GC
Cuts at: 3426 3426
Size: 6463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 3582 4588 5453 5751 2201
Size: 1381 1006 865 298 2913
Fragments arranged by size:
2913 1381 1006 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3689 5126 882
Size: 617 2190 1437 2219
Fragments arranged by size:
2219 2190 1437 617
BsaAI yAC'GTr
Cuts at: 493 1204 4254 493
Size: 711 3050 2702
Fragments arranged by size:
3050 2702 711
BsaBI GATnn'nnATC
Cuts at: 2196 2970 6457 2196
Size: 774 3487 2202
Fragments arranged by size:
3487 2202 774
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 4270 4652 5287
Size: 53 83 186 936 552 2185 382 635
Cuts at: 5287 275
Size: 1451
Fragments arranged by size:
2185 1451 936 635 552 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4896 5294 5568 5715 6126 4896
Size: 398 274 147 411 5233
Fragments arranged by size:
5233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2358 2388 1178
Size: 30 165 30 955 30 5253
Fragments arranged by size:
5253 955 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 2535 1186
Size: 939 410 5114
Fragments arranged by size:
5114 939 410
BseYI C'CCAG_C
Cuts at: 5666 6162 5666
Size: 496 5967
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5890
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3913 4674 5702 1540
Size: 275 186 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2540 3758 4524 4609 5680 2025
Size: 515 1218 766 85 1071 2808
Fragments arranged by size:
2808 1218 1071 766 515 85
BsmI GAATG_Cn'
Cuts at: 2076 2728 3355 3974 4067 2076
Size: 652 627 619 93 4472
Fragments arranged by size:
4472 652 627 619 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2325 2325
Size: 6463
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2043 2316 2540 3680 3758 3782
Size: 399 235 18 273 224 1140 78 24
Cuts at: 3782 4524 4609 5680 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1140 1071 742 399 273 235 224
85 78 24 18
BspEI T'CCGG_A
Cuts at: 6126 6126
Size: 6463
BspHI T'CATG_A
Cuts at: 3033 4352 6082 3033
Size: 1319 1730 3414
Fragments arranged by size:
3414 1730 1319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3818 844
Size: 2974 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4350 1394
Size: 429 2527 3507
Fragments arranged by size:
3507 2527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4958 5132 65
Size: 1183 3710 174 1396
Fragments arranged by size:
3710 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 5107 5294 1700
Size: 110 319 2978 187 2869
Fragments arranged by size:
2978 2869 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6463
BssSI C'ACGA_G
Cuts at: 3102 4523 5535 3102
Size: 1421 1012 4030
Fragments arranged by size:
4030 1421 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6463
BstBI TT'CG_AA
Cuts at: 5267 5267
Size: 6463
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstYI r'GATC_y
Cuts at: 1972 2185 2815 3271 3462 3793 4160 4545 4562
Size: 213 630 456 191 331 367 385 17
Cuts at: 4562 6014 6100 6112 6458 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 630 456 385 367 346 331
213 191 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2976 2976
Size: 6463
BtgI C'CryG_G
Cuts at: 513 1065 2004 3017 3672 513
Size: 552 939 1013 655 3304
Fragments arranged by size:
3304 1013 939 655 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5724
Fragments arranged by size:
5724 683 56
BtsI GCAGTG_nn'
Cuts at: 2634 3235 3589 3979 4773 4793 2634
Size: 601 354 390 794 20 4304
Fragments arranged by size:
4304 794 601 390 354 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6428
DraI TTT'AAA
Cuts at: 3767 3767
Size: 6463
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5470 809
Size: 507 4154 1802
Fragments arranged by size:
4154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2633 3910 4799
Size: 54 1425 50 275 18 803 1277 889
Cuts at: 4799 6428 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1277 889 803 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3910 1537
Size: 275 2098 4090
Fragments arranged by size:
4090 2098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 3708 4393 1353
Size: 1233 1122 685 3423
Fragments arranged by size:
3423 1233 1122 685
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2655 3011 5050 5424 5570 387
Size: 1064 138 1066 356 2039 374 146 1280
Fragments arranged by size:
2039 1280 1066 1064 374 356 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2156 2379 2385 2502 2670 4526
Size: 393 455 69 223 6 117 168 1856
Cuts at: 4526 5123 5910 6178 1239
Size: 597 787 268 1524
Fragments arranged by size:
1856 1524 787 597 455 393 268 223
168 117 69 6
EcoO109I rG'GnC_Cy
Cuts at: 3109 3109
Size: 6463
EcoRI G'AATT_C
Cuts at: 2728 3772 4150 2728
Size: 1044 378 5041
Fragments arranged by size:
5041 1044 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2706 2706
Size: 6463
FspI TGC'GCA
Cuts at: 4969 4969
Size: 6463
HaeII r_GCGC'y
Cuts at: 1678 2088 5610 1678
Size: 410 3522 2531
Fragments arranged by size:
3522 2531 410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2358 2390 2603 2635 3052
Size: 32 135 32 786 32 213 32 417
Cuts at: 3052 3084 3256 3273 3288 3305 3718 3750 5104
Size: 32 172 17 15 17 413 32 1354
Cuts at: 5104 5136 5178 5210 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1354 786 417 413 213 172 135
42 32 32 32 32 32 32 32
32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2792 3807 4053 669
Size: 988 281 854 1015 246 3079
Fragments arranged by size:
3079 1015 988 854 281 246
HindIII A'AGCT_T
Cuts at: 748 2688 748
Size: 1940 4523
HpaI GTT'AAC
Cuts at: 4053 4053
Size: 6463
KasI G'GCGC_C
Cuts at: 2084 2084
Size: 6463
KpnI G_GTAC'C
Cuts at: 3788 3788
Size: 6463
MfeI C'AATT_G
Cuts at: 2295 4062 2295
Size: 1767 4696
MluI A'CGCG_T
Cuts at: 6339 6339
Size: 6463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3038 3173 5577 5761 1305
Size: 1733 135 2404 184 2007
Fragments arranged by size:
2404 2007 1733 184 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2635 10
Size: 54 1425 343 803 3838
Fragments arranged by size:
3838 1425 803 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4421 4780 4939 518
Size: 648 843 2412 359 159 2042
Fragments arranged by size:
2412 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 3879 4556 5704 5949 1606
Size: 494 1779 677 1148 245 2120
Fragments arranged by size:
2120 1779 1148 677 494 245
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 6463
NarI GG'CG_CC
Cuts at: 2085 2085
Size: 6463
NcoI C'CATG_G
Cuts at: 513 1065 2004 3017 3672 513
Size: 552 939 1013 655 3304
Fragments arranged by size:
3304 1013 939 655 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6463
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 6463
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6463
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 3083 3821 5366 1132
Size: 97 1854 738 1545 2229
Fragments arranged by size:
2229 1854 1545 738 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6463
PciI A'CATG_T
Cuts at: 5362 5362
Size: 6463
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6463
PfoI T'CCnGG_A
Cuts at: 2112 2112
Size: 6463
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4564 6045 6077 4532
Size: 32 1481 32 4918
Fragments arranged by size:
4918 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3109 3109
Size: 6463
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 2454 4033 2454
Size: 1579 4884
PstI C_TGCA'G
Cuts at: 830 2629 3559 3815 830
Size: 1799 930 256 3478
Fragments arranged by size:
3478 1799 930 256
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
SalI G'TCGA_C
Cuts at: 1936 2790 3805 1936
Size: 854 1015 4594
Fragments arranged by size:
4594 1015 854
SapI GCTCTTCn'nnn_
Cuts at: 2586 2586
Size: 6463
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 4711 6423 4711
Size: 1712 4751
SfcI C'TryA_G
Cuts at: 826 1045 2625 3555 3811 4946 5627 5818 826
Size: 219 1580 930 256 1135 681 191 1471
Fragments arranged by size:
1580 1471 1135 930 681 256 219 191
SfoI GGC'GCC
Cuts at: 2086 2086
Size: 6463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 3790 1872
Size: 1918 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2180 3597 4567 5468 5730 820
Size: 197 926 237 1417 970 901 262 1553
Fragments arranged by size:
1553 1417 970 926 901 262 237 197
SnaBI TAC'GTA
Cuts at: 493 4254 493
Size: 3761 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6463
SphI G_CATG'C
Cuts at: 1132 3083 3821 1132
Size: 1951 738 3774
Fragments arranged by size:
3774 1951 738
SspI AAT'ATT
Cuts at: 5 52 4387 5
Size: 47 4335 2081
Fragments arranged by size:
4335 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6463
StyI C'CwwG_G
Cuts at: 513 1065 2004 2960 3017 3672 3902 513
Size: 552 939 956 57 655 230 3074
Fragments arranged by size:
3074 956 939 655 552 230 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2683 4490 4507 4660 4845 5290 325
Size: 1255 1103 1807 17 153 185 445 1498
Fragments arranged by size:
1807 1498 1255 1103 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4709 6421 96
Size: 274 80 33 51 1443 2732 1712 138
Fragments arranged by size:
2732 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6423 6455 6423
Size: 32 6431
Tth111I GACn'n_nGTC
Cuts at: 1316 2757 3495 1316
Size: 1441 738 4284
Fragments arranged by size:
4284 1441 738
XbaI T'CTAG_A
Cuts at: 3799 3799
Size: 6463
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6463
XmaI C'CCGG_G
Cuts at: 1870 3788 1870
Size: 1918 4545
XmnI GAAnn'nnTTC
Cuts at: 4592 4592
Size: 6463
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI XcmI