Restriction map for
pFN21AB6122
(Circular) (Six-base) MAPSORT of: pFN21AB6122.seq Check: 9285 from: 1 to: 6457
pFN21AB6122 6457 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 278
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3800 1937
Size: 1863 4594
Acc65I G'GTAC_C
Cuts at: 3778 3778
Size: 6457
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2654 2689 2732 2856 2873 3033 4520 5904
Size: 1022 35 43 124 17 160 1487 1384
Cuts at: 5904 6172 1632
Size: 268 1917
Fragments arranged by size:
1917 1487 1384 1022 268 160 124 43
35 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6260
AflIII A'CryG_T
Cuts at: 1203 5356 6333 1203
Size: 4153 977 1327
Fragments arranged by size:
4153 1327 977
AgeI A'CCGG_T
Cuts at: 5288 5288
Size: 6457
AhdI GACnn_n'nnGTC
Cuts at: 5186 5186
Size: 6457
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6457
AlwNI CAG_nnn'CTG
Cuts at: 2461 4200 5772 2461
Size: 1739 1572 3146
Fragments arranged by size:
3146 1739 1572
ApaLI G'TGCA_C
Cuts at: 4514 5670 4514
Size: 1156 5301
ApoI r'AATT_y
Cuts at: 1493 2109 3339 3766 3992 4144 6253 1493
Size: 616 1230 427 226 152 2109 1697
Fragments arranged by size:
2109 1697 1230 616 427 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 2932 3782 1870
Size: 73 989 850 4545
Fragments arranged by size:
4545 989 850 73
BamHI G'GATC_C
Cuts at: 3787 4154 3787
Size: 367 6090
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3778 5233 618
Size: 325 217 627 1991 1455 1842
Fragments arranged by size:
1991 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2189 2213 2503 3776 1391
Size: 798 24 290 1273 4072
Fragments arranged by size:
4072 1273 798 290 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3726 928
Size: 971 1827 3659
Fragments arranged by size:
3659 1827 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3248 3282 4648 4682 1903
Size: 34 1311 34 1366 34 3678
Fragments arranged by size:
3678 1366 1311 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4349 5559 3665
Size: 684 1210 4563
Fragments arranged by size:
4563 1210 684
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2690 3182 3480
Size: 107 122 71 631 605 1018 492 298
Cuts at: 3480 3573 5068 136
Size: 93 1495 1525
Fragments arranged by size:
1525 1495 1018 631 605 492 298 122
107 93 71
BglII A'GATC_T
Cuts at: 6452 6452
Size: 6457
BlpI GC'TnA_GC
Cuts at: 3874 3874
Size: 6457
Bme1580I G_kGCm'C
Cuts at: 1790 4518 5674 1790
Size: 2728 1156 2573
Fragments arranged by size:
2728 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4188 5146 442
Size: 416 53 83 399 24 2771 958 1753
Fragments arranged by size:
2771 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2831 3149 3350 3541 3673 5117 1239
Size: 1592 318 201 191 132 1444 2579
Fragments arranged by size:
2579 1592 1444 318 201 191 132
Bpu10I CC'TnA_GC
Cuts at: 2149 2149
Size: 6457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2008 4582 5447 5745 2008
Size: 2574 865 298 2720
Fragments arranged by size:
2720 2574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5120 882
Size: 617 3621 2219
Fragments arranged by size:
3621 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4248 493
Size: 711 3044 2702
Fragments arranged by size:
3044 2702 711
BsaBI GATnn'nnATC
Cuts at: 6451 6451
Size: 6457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4264 4646 5281 275
Size: 53 83 186 936 2731 382 635 1451
Fragments arranged by size:
2731 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3380 4890 5288 5562 5709 6120 3380
Size: 1510 398 274 147 411 3717
Fragments arranged by size:
3717 1510 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2079 2109 2176
Size: 30 165 30 624 30 22 30 67
Cuts at: 2176 2206 3692 3722 1178
Size: 30 1486 30 3913
Fragments arranged by size:
3913 1486 624 165 67 30 30 30
30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2070 2073 2076 2079 2082 2085 2097 2100
Size: 884 3 3 3 3 3 12 3
Cuts at: 2100 2103 2106 2403 2427 2882 3700 1186
Size: 3 3 297 24 455 818 3943
Fragments arranged by size:
3943 884 818 455 297 24 12 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 5660 6156 5660
Size: 496 5961
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3230 1163
Size: 573 1494 4390
Fragments arranged by size:
4390 1494 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3907 4668 5696 1540
Size: 275 186 1906 761 1028 2301
Fragments arranged by size:
2301 1906 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2189 4518 4603 5674 2189
Size: 2329 85 1071 2972
Fragments arranged by size:
2972 2329 1071 85
BsmI GAATG_Cn'
Cuts at: 2929 3968 4061 2929
Size: 1039 93 5325
Fragments arranged by size:
5325 1039 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3103 3103
Size: 6457
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2213 2503 3776 4518 4603 5674
Size: 399 399 24 290 1273 742 85 1071
Cuts at: 5674 1391
Size: 2174
Fragments arranged by size:
2174 1273 1071 742 399 399 290 85
24
BspEI T'CCGG_A
Cuts at: 3380 6120 3380
Size: 2740 3717
BspHI T'CATG_A
Cuts at: 4346 6076 4346
Size: 1730 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3812 844
Size: 2968 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3242 4344 1394
Size: 429 1419 1102 3507
Fragments arranged by size:
3507 1419 1102 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4952 5126 65
Size: 1183 3704 174 1396
Fragments arranged by size:
3704 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5101 5288 1700
Size: 110 3291 187 2869
Fragments arranged by size:
3291 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3118 96
Size: 3022 3435
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 2583 2864 4517 5529 2583
Size: 281 1653 1012 3511
Fragments arranged by size:
3511 1653 1012 281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6457
BstBI TT'CG_AA
Cuts at: 2887 5261 2887
Size: 2374 4083
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6457
BstYI r'GATC_y
Cuts at: 1972 3321 3787 4154 4539 4556 6008 6094 6106
Size: 1349 466 367 385 17 1452 86 12
Cuts at: 6106 6452 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1349 466 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2179 3475 3568 3723 513
Size: 552 1114 1296 93 155 3247
Fragments arranged by size:
3247 1296 1114 552 155 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5718
Fragments arranged by size:
5718 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 3973 4767 4787 2056
Size: 1917 794 20 3726
Fragments arranged by size:
3726 1917 794 20
ClaI AT'CG_AT
Cuts at: 1554 2613 1554
Size: 1059 5398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6422
DraI TTT'AAA
Cuts at: 3761 3761
Size: 6457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5464 809
Size: 507 4148 1802
Fragments arranged by size:
4148 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3472 3565 3904
Size: 54 1425 50 275 18 1642 93 339
Cuts at: 3904 4793 6422 8
Size: 889 1629 43
Fragments arranged by size:
1642 1629 1425 889 339 275 93 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3904 1537
Size: 275 2092 4090
Fragments arranged by size:
4090 2092 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2044 2832 3718 4387 1353
Size: 691 788 886 669 3423
Fragments arranged by size:
3423 886 788 691 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3405 3417 3444 3459 3522 3537
Size: 1064 138 1816 12 27 15 63 15
Cuts at: 3537 3564 3684 5044 5418 5564 387
Size: 27 120 1360 374 146 1280
Fragments arranged by size:
1816 1360 1280 1064 374 146 138 120
63 27 27 15 15 12
EcoICRI GAG'CTC
Cuts at: 2187 2187
Size: 6457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2654 2689 2732 2831 2856 2873 3033
Size: 393 1022 35 43 99 25 17 160
Cuts at: 3033 3149 3350 3541 3673 4520 5117 5904 6172
Size: 116 201 191 132 847 597 787 268
Cuts at: 6172 1239
Size: 1524
Fragments arranged by size:
1524 1022 847 787 597 393 268 201
191 160 132 116 99 43 35 25
17
EcoNI CCTnn'n_nnAGG
Cuts at: 2798 2798
Size: 6457
EcoRI G'AATT_C
Cuts at: 3766 4144 3766
Size: 378 6079
EcoRV GAT'ATC
Cuts at: 3078 3237 3078
Size: 159 6298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2147 2147
Size: 6457
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 5604 1678
Size: 3926 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3226 3258 3722 3754 5098
Size: 32 135 32 1654 32 464 32 1344
Cuts at: 5098 5130 5172 5204 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1654 1344 464 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3801 4047 669
Size: 988 281 1863 246 3079
Fragments arranged by size:
3079 1863 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6457
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KpnI G_GTAC'C
Cuts at: 3782 3782
Size: 6457
MfeI C'AATT_G
Cuts at: 4056 4056
Size: 6457
MluI A'CGCG_T
Cuts at: 6333 6333
Size: 6457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3349 5571 5755 1305
Size: 2044 2222 184 2007
Fragments arranged by size:
2222 2044 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4635
Fragments arranged by size:
4635 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4415 4774 4933 518
Size: 648 3249 359 159 2042
Fragments arranged by size:
3249 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2696 3561 3585 3693 3873 4550 5698 5943
Size: 1090 865 24 108 180 677 1148 245
Cuts at: 5943 1606
Size: 2120
Fragments arranged by size:
2120 1148 1090 865 677 245 180 108
24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5905
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6457
NotI GC'GGCC_GC
Cuts at: 3904 3904
Size: 6457
NspI r_CATG'y
Cuts at: 1132 1229 3815 5360 1132
Size: 97 2586 1545 2229
Fragments arranged by size:
2586 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6457
PciI A'CATG_T
Cuts at: 5356 5356
Size: 6457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2746 1108
Size: 1638 4819
PfoI T'CCnGG_A
Cuts at: 3309 3309
Size: 6457
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 6039 6071 4526
Size: 32 1481 32 4912
Fragments arranged by size:
4912 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6457
PsiI TTA'TAA
Cuts at: 4027 4027
Size: 6457
PstI C_TGCA'G
Cuts at: 830 3809 830
Size: 2979 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6457
PvuII CAG'CTG
Cuts at: 2696 2696
Size: 6457
SacI G_AGCT'C
Cuts at: 2189 2189
Size: 6457
SalI G'TCGA_C
Cuts at: 1936 3799 1936
Size: 1863 4594
SapI GCTCTTCn'nnn_
Cuts at: 2832 2832
Size: 6457
SbfI CC_TGCA'GG
Cuts at: 3809 3809
Size: 6457
ScaI AGT'ACT
Cuts at: 4705 6417 4705
Size: 1712 4745
SfcI C'TryA_G
Cuts at: 826 1045 2590 3048 3140 3805 4940 5621 5812
Size: 219 1545 458 92 665 1135 681 191
Cuts at: 5812 826
Size: 1471
Fragments arranged by size:
1545 1471 1135 681 665 458 219 191
92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6457
SmaI CCC'GGG
Cuts at: 1872 2934 3784 1872
Size: 1062 850 4545
Fragments arranged by size:
4545 1062 850
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2023 4561 5462 5724 820
Size: 197 926 80 2538 901 262 1553
Fragments arranged by size:
2538 1553 926 901 262 197 80
SnaBI TAC'GTA
Cuts at: 493 4248 493
Size: 3755 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 3815 1132
Size: 2683 3774
SspI AAT'ATT
Cuts at: 5 52 4381 5
Size: 47 4329 2081
Fragments arranged by size:
4329 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6457
StyI C'CwwG_G
Cuts at: 513 1065 2131 2799 3180 3896 513
Size: 552 1066 668 381 716 3074
Fragments arranged by size:
3074 1066 716 668 552 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4484 4501 4654 4839 5284 325
Size: 1255 2904 17 153 185 445 1498
Fragments arranged by size:
2904 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3118 4703 6415
Size: 274 80 33 51 1443 1141 1585 1712
Cuts at: 6415 96
Size: 138
Fragments arranged by size:
1712 1585 1443 1141 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 2079 2111 2176 2208 3692 3724 6417
Size: 32 23 32 65 32 1484 32 2693
Cuts at: 6417 6449 2024
Size: 32 2032
Fragments arranged by size:
2693 2032 1484 65 32 32 32 32
32 23
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6457
XbaI T'CTAG_A
Cuts at: 3793 3793
Size: 6457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2806 2806
Size: 6457
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6457
XmaI C'CCGG_G
Cuts at: 1870 2932 3782 1870
Size: 1062 850 4545
Fragments arranged by size:
4545 1062 850
XmnI GAAnn'nnTTC
Cuts at: 4586 4586
Size: 6457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 276
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI