Restriction map for
pFN21AB6118
(Circular) (Six-base) MAPSORT of: pFN21AB6118.seq Check: 8587 from: 1 to: 6343
pFN21AB6118 6343 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2398 2398
Size: 6343
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4153 278
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2532 3686 1937
Size: 595 1154 4594
Fragments arranged by size:
4594 1154 595
Acc65I G'GTAC_C
Cuts at: 3664 3664
Size: 6343
AclI AA'CG_TT
Cuts at: 1563 4470 4843 1563
Size: 2907 373 3063
Fragments arranged by size:
3063 2907 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4406 5790 6058 1632
Size: 2774 1384 268 1917
Fragments arranged by size:
2774 1917 1384 268
AfeI AGC'GCT
Cuts at: 2304 2304
Size: 6343
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6146
AflIII A'CryG_T
Cuts at: 1203 5242 6219 1203
Size: 4039 977 1327
Fragments arranged by size:
4039 1327 977
AgeI A'CCGG_T
Cuts at: 5174 5174
Size: 6343
AhdI GACnn_n'nnGTC
Cuts at: 5072 5072
Size: 6343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3048 1184
Size: 1864 4479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 6311
AlwNI CAG_nnn'CTG
Cuts at: 2185 2353 3363 4086 5658 2185
Size: 168 1010 723 1572 2870
Fragments arranged by size:
2870 1572 1010 723 168
ApaI G_GGCC'C
Cuts at: 2994 3232 2994
Size: 238 6105
ApaLI G'TGCA_C
Cuts at: 2411 2498 4400 5556 2411
Size: 87 1902 1156 3198
Fragments arranged by size:
3198 1902 1156 87
ApoI r'AATT_y
Cuts at: 1493 3029 3632 3652 3878 4030 6139 1493
Size: 1536 603 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 1536 603 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 4897 160
Size: 872 3865 1606
Fragments arranged by size:
3865 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2986 3611 3668 1870
Size: 73 110 933 625 57 4545
Fragments arranged by size:
4545 933 625 110 73 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2916 2949 2916
Size: 33 6310
BamHI G'GATC_C
Cuts at: 2217 3673 4040 2217
Size: 1456 367 4520
Fragments arranged by size:
4520 1456 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2388 2698 3018 3161
Size: 325 217 627 270 331 310 320 143
Cuts at: 3161 3664 5119 618
Size: 503 1455 1842
Fragments arranged by size:
1842 1455 627 503 331 325 320 310
270 217 143
BanII G_rGCy'C
Cuts at: 1391 2597 2751 2994 3232 3662 1391
Size: 1206 154 243 238 430 4072
Fragments arranged by size:
4072 1206 430 243 238 154
BbeI G_GCGC'C
Cuts at: 2061 2392 2061
Size: 331 6012
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3456 928
Size: 971 1557 3815
Fragments arranged by size:
3815 1557 971
BbvCI CC'TCA_GC
Cuts at: 3130 3475 3130
Size: 345 5998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2497 2531 2534 2568 4534 4568 1903
Size: 34 560 34 3 34 1966 34 3678
Fragments arranged by size:
3678 1966 560 34 34 34 34 3
BciVI GTATCCnnnnn_n'
Cuts at: 4235 5445 4235
Size: 1210 5133
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6343
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2386 3016 3434
Size: 107 122 71 631 605 714 630 418
Cuts at: 3434 4954 136
Size: 1520 1525
Fragments arranged by size:
1525 1520 714 631 630 605 418 122
107 71
BglII A'GATC_T
Cuts at: 6338 6338
Size: 6343
BlpI GC'TnA_GC
Cuts at: 2295 3760 2295
Size: 1465 4878
Bme1580I G_kGCm'C
Cuts at: 1790 2415 2502 2696 2703 2994 3021 3166 3174
Size: 625 87 194 7 291 27 145 8
Cuts at: 3174 3232 4404 5560 1790
Size: 58 1172 1156 2573
Fragments arranged by size:
2573 1172 1156 625 291 194 145 87
58 27 8 7
BmgBI CAC'GTC
Cuts at: 1318 3244 1318
Size: 1926 4417
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3300 3373 4074
Size: 416 53 83 399 24 1883 73 701
Cuts at: 4074 5032 442
Size: 958 1753
Fragments arranged by size:
1883 1753 958 701 416 399 83 73
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2741 5003 1239
Size: 1502 2262 2579
Fragments arranged by size:
2579 2262 1502
Bpu10I CC'TnA_GC
Cuts at: 3130 3475 3130
Size: 345 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4468 5333 5631 4468
Size: 865 298 5180
Fragments arranged by size:
5180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 3292 5006 882
Size: 617 558 1235 1714 2219
Fragments arranged by size:
2219 1714 1235 617 558
BsaAI yAC'GTr
Cuts at: 493 1204 4134 493
Size: 711 2930 2702
Fragments arranged by size:
2930 2702 711
BsaBI GATnn'nnATC
Cuts at: 6337 6337
Size: 6343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2261 2389 2474
Size: 53 83 186 936 525 203 128 85
Cuts at: 2474 4150 4532 5167 275
Size: 1676 382 635 1451
Fragments arranged by size:
1676 1451 936 635 525 382 203 186
128 85 83 53
BsaWI w'CCGG_w
Cuts at: 2066 2586 2997 4776 5174 5448 5595 6006 2066
Size: 520 411 1779 398 274 147 411 2403
Fragments arranged by size:
2403 1779 520 411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 3191 3221 1178
Size: 30 165 30 675 30 1083 30 4300
Fragments arranged by size:
4300 1083 675 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2345 2605 2701 3118 3194 3463 3520
Size: 862 297 260 96 417 76 269 57
Cuts at: 3520 1186
Size: 4009
Fragments arranged by size:
4009 862 417 297 269 260 96 76
57
BseYI C'CCAG_C
Cuts at: 2178 2597 3499 3506 5546 6042 2178
Size: 419 902 7 2040 496 2479
Fragments arranged by size:
2479 2040 902 496 419 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 3285 1163
Size: 573 609 940 4221
Fragments arranged by size:
4221 940 609 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2419 3793 4554 5582 1540
Size: 275 186 418 1374 761 1028 2301
Fragments arranged by size:
2301 1374 1028 761 418 275 186
BsiHKAI G_wGCw'C
Cuts at: 2415 2502 2751 2927 4404 4489 5560 2415
Size: 87 249 176 1477 85 1071 3198
Fragments arranged by size:
3198 1477 1071 249 176 87 85
BsmI GAATG_Cn'
Cuts at: 3854 3947 3854
Size: 93 6250
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2415 2502 2597 2696 2703 2751 2927
Size: 399 625 87 95 99 7 48 176
Cuts at: 2927 2994 3021 3166 3174 3232 3662 4404 4489
Size: 67 27 145 8 58 430 742 85
Cuts at: 4489 5560 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 625 430 399 176 145
99 95 87 85 67 58 48 27
8 7
BspEI T'CCGG_A
Cuts at: 2586 2997 6006 2586
Size: 411 3009 2923
Fragments arranged by size:
3009 2923 411
BspHI T'CATG_A
Cuts at: 4232 5962 4232
Size: 1730 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2398 2442 3698 844
Size: 1554 44 1256 3489
Fragments arranged by size:
3489 1554 1256 44
BsrBI CCG'CTC
Cuts at: 1394 1823 2971 4230 1394
Size: 429 1148 1259 3507
Fragments arranged by size:
3507 1259 1148 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4838 5012 65
Size: 1183 3590 174 1396
Fragments arranged by size:
3590 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2418 4987 5174 1700
Size: 110 608 2569 187 2869
Fragments arranged by size:
2869 2569 608 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6343
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6343
BssSI C'ACGA_G
Cuts at: 2098 2750 3486 4403 5415 2098
Size: 652 736 917 1012 3026
Fragments arranged by size:
3026 1012 917 736 652
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2353 3299 794
Size: 1559 946 3838
Fragments arranged by size:
3838 1559 946
BstBI TT'CG_AA
Cuts at: 5147 5147
Size: 6343
BstEII G'GTnAC_C
Cuts at: 1184 3415 1184
Size: 2231 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 2948 2948
Size: 6343
BstYI r'GATC_y
Cuts at: 1972 2217 2349 2826 3673 4040 4425 4442 5894
Size: 245 132 477 847 367 385 17 1452
Cuts at: 5894 5980 5992 6338 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 847 477 385 367 346 245
132 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2564 2564
Size: 6343
BtgI C'CryG_G
Cuts at: 513 1065 2004 3105 513
Size: 552 939 1101 3751
Fragments arranged by size:
3751 1101 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5604
Fragments arranged by size:
5604 683 56
BtsI GCAGTG_nn'
Cuts at: 2110 2369 2925 3395 3859 4653 4673 2110
Size: 259 556 470 464 794 20 3780
Fragments arranged by size:
3780 794 556 470 464 259 20
ClaI AT'CG_AT
Cuts at: 1554 3213 1554
Size: 1659 4684
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6308
DraI TTT'AAA
Cuts at: 3647 3647
Size: 6343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2071 5350 809
Size: 507 755 3279 1802
Fragments arranged by size:
3279 1802 755 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2416 3196 3790
Size: 54 1425 50 275 18 586 780 594
Cuts at: 3790 4679 6308 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 780 594 586 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2416 3790 1537
Size: 275 604 1374 4090
Fragments arranged by size:
4090 1374 604 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 2337 4273 1353
Size: 762 222 1936 3423
Fragments arranged by size:
3423 1936 762 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2290 4930 5304 5450 387
Size: 1064 138 701 2640 374 146 1280
Fragments arranged by size:
2640 1280 1064 701 374 146 138
EcoICRI GAG'CTC
Cuts at: 2749 2749
Size: 6343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2741 4406 5003 5790 6058 1239
Size: 393 1109 1665 597 787 268 1524
Fragments arranged by size:
1665 1524 1109 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 2327 2834 2189
Size: 138 507 5698
Fragments arranged by size:
5698 507 138
EcoO109I rG'GnC_Cy
Cuts at: 2131 2990 3113 3228 2131
Size: 859 123 115 5246
Fragments arranged by size:
5246 859 123 115
EcoRI G'AATT_C
Cuts at: 3632 3652 4030 3632
Size: 20 378 5945
Fragments arranged by size:
5945 378 20
FspI TGC'GCA
Cuts at: 4849 4849
Size: 6343
HaeII r_GCGC'y
Cuts at: 1678 2061 2295 2306 2392 2797 5490 1678
Size: 383 234 11 86 405 2693 2531
Fragments arranged by size:
2693 2531 405 383 234 86 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4984 5016 5058 5090 1373
Size: 32 135 32 3412 32 42 32 2626
Fragments arranged by size:
3412 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3687 3933 669
Size: 988 281 1749 246 3079
Fragments arranged by size:
3079 1749 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6343
HpaI GTT'AAC
Cuts at: 3933 3933
Size: 6343
KasI G'GCGC_C
Cuts at: 2057 2388 2057
Size: 331 6012
KpnI G_GTAC'C
Cuts at: 3668 3668
Size: 6343
MfeI C'AATT_G
Cuts at: 3942 3942
Size: 6343
MluI A'CGCG_T
Cuts at: 6219 6219
Size: 6343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 2664 5457 5641 1305
Size: 795 564 2793 184 2007
Fragments arranged by size:
2793 2007 795 564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3198 10
Size: 54 1425 343 1366 3155
Fragments arranged by size:
3155 1425 1366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3184 4301 4660 4819 518
Size: 648 2018 1117 359 159 2042
Fragments arranged by size:
2042 2018 1117 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2236 2359 2578 3134 3759 4436 5584 5829
Size: 630 123 219 556 625 677 1148 245
Cuts at: 5829 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 630 625 556 245 219
123
NarI GG'CG_CC
Cuts at: 2058 2389 2058
Size: 331 6012
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4852
Fragments arranged by size:
4852 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6343
NotI GC'GGCC_GC
Cuts at: 3790 3790
Size: 6343
NspI r_CATG'y
Cuts at: 1132 1229 3586 3701 5246 1132
Size: 97 2357 115 1545 2229
Fragments arranged by size:
2357 2229 1545 115 97
PasI CC'CwG_GG
Cuts at: 1348 2168 2468 3167 3596 1348
Size: 820 300 699 429 4095
Fragments arranged by size:
4095 820 699 429 300
PciI A'CATG_T
Cuts at: 5242 5242
Size: 6343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6343
PmeI GTTT'AAAC
Cuts at: 3647 3647
Size: 6343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 5925 5957 4412
Size: 32 1481 32 4798
Fragments arranged by size:
4798 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2131 2131
Size: 6343
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6343
PsiI TTA'TAA
Cuts at: 3913 3913
Size: 6343
PspOMI G'GGCC_C
Cuts at: 2990 3228 2990
Size: 238 6105
PstI C_TGCA'G
Cuts at: 830 2190 2328 2358 2376 2444 3320 3695 830
Size: 1360 138 30 18 68 876 375 3478
Fragments arranged by size:
3478 1360 876 375 138 68 30 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6343
PvuII CAG'CTG
Cuts at: 2359 2578 3134 2359
Size: 219 556 5568
Fragments arranged by size:
5568 556 219
SacI G_AGCT'C
Cuts at: 2751 2751
Size: 6343
SalI G'TCGA_C
Cuts at: 1936 3685 1936
Size: 1749 4594
SapI GCTCTTCn'nnn_
Cuts at: 2337 2337
Size: 6343
SbfI CC_TGCA'GG
Cuts at: 2190 2328 3695 2190
Size: 138 1367 4838
Fragments arranged by size:
4838 1367 138
ScaI AGT'ACT
Cuts at: 4591 6303 4591
Size: 1712 4631
SfcI C'TryA_G
Cuts at: 826 1045 2080 2186 2324 2354 2372 2440 2834
Size: 219 1035 106 138 30 18 68 394
Cuts at: 2834 3316 3691 4826 5507 5698 826
Size: 482 375 1135 681 191 1471
Fragments arranged by size:
1471 1135 1035 681 482 394 375 219
191 138 106 68 30 18
SfoI GGC'GCC
Cuts at: 2059 2390 2059
Size: 331 6012
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6343
SmaI CCC'GGG
Cuts at: 1872 2988 3613 3670 1872
Size: 1116 625 57 4545
Fragments arranged by size:
4545 1116 625 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4447 5348 5610 820
Size: 197 926 2504 901 262 1553
Fragments arranged by size:
2504 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4134 493
Size: 3641 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6343
SphI G_CATG'C
Cuts at: 1132 3701 1132
Size: 2569 3774
SspI AAT'ATT
Cuts at: 5 52 4267 5
Size: 47 4215 2081
Fragments arranged by size:
4215 2081 47
StuI AGG'CCT
Cuts at: 1517 3006 3255 3428 1517
Size: 1489 249 173 4432
Fragments arranged by size:
4432 1489 249 173
StyI C'CwwG_G
Cuts at: 513 1065 2004 2286 3782 513
Size: 552 939 282 1496 3074
Fragments arranged by size:
3074 1496 939 552 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3319 4370 4387 4540 4725 5170 325
Size: 1255 1739 1051 17 153 185 445 1498
Fragments arranged by size:
1739 1498 1255 1051 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2878 4589 6301
Size: 274 80 33 51 1443 901 1711 1712
Cuts at: 6301 96
Size: 138
Fragments arranged by size:
1712 1711 1443 901 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 3191 3223 3365 3397 6303 6335 2078
Size: 32 1081 32 142 32 2906 32 2086
Fragments arranged by size:
2906 2086 1081 142 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2204 1316
Size: 888 5455
XbaI T'CTAG_A
Cuts at: 3679 3679
Size: 6343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2647 2647
Size: 6343
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6343
XmaI C'CCGG_G
Cuts at: 1870 2986 3611 3668 1870
Size: 1116 625 57 4545
Fragments arranged by size:
4545 1116 625 57
XmnI GAAnn'nnTTC
Cuts at: 4472 4472
Size: 6343
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4151 276
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I DraIII
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI