Restriction map for
pFN21AB6117
(Circular) (Six-base) MAPSORT of: pFN21AB6117.seq Check: 3169 from: 1 to: 6568
pFN21AB6117 6568 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3911 1937
Size: 1974 4594
Acc65I G'GTAC_C
Cuts at: 3889 3889
Size: 6568
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2799 4631 6015 6283 1632
Size: 678 489 1832 1384 268 1917
Fragments arranged by size:
1917 1832 1384 678 489 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6371
AflIII A'CryG_T
Cuts at: 1203 5467 6444 1203
Size: 4264 977 1327
Fragments arranged by size:
4264 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6568
AlwNI CAG_nnn'CTG
Cuts at: 2996 3374 4311 5883 2996
Size: 378 937 1572 3681
Fragments arranged by size:
3681 1572 937 378
ApaLI G'TGCA_C
Cuts at: 4625 5781 4625
Size: 1156 5412
ApoI r'AATT_y
Cuts at: 1493 2832 3219 3254 3877 4103 4255 6364 1493
Size: 1339 387 35 623 226 152 2109 1697
Fragments arranged by size:
2109 1697 1339 623 387 226 152 35
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 3893 1870
Size: 73 1950 4545
Fragments arranged by size:
4545 1950 73
BamHI G'GATC_C
Cuts at: 3898 4265 3898
Size: 367 6201
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3544 3889 5344 618
Size: 325 217 627 1757 345 1455 1842
Fragments arranged by size:
1842 1757 1455 627 345 325 217
BanII G_rGCy'C
Cuts at: 1391 3887 1391
Size: 2496 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3456 928
Size: 971 1557 4040
Fragments arranged by size:
4040 1557 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4759 4793 1903
Size: 34 2822 34 3678
Fragments arranged by size:
3678 2822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3040 3322 4460 5670 3040
Size: 282 1138 1210 3938
Fragments arranged by size:
3938 1210 1138 282
BclI T'GATC_A
Cuts at: 1548 2953 1548
Size: 1405 5163
BfrBI ATG'CAT
Cuts at: 2580 3474 2580
Size: 894 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3386 5179 136
Size: 107 122 71 631 605 1714 1793 1525
Fragments arranged by size:
1793 1714 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6563 6563
Size: 6568
BlpI GC'TnA_GC
Cuts at: 3985 3985
Size: 6568
Bme1580I G_kGCm'C
Cuts at: 1790 3044 3549 4629 5785 1790
Size: 1254 505 1080 1156 2573
Fragments arranged by size:
2573 1254 1156 1080 505
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3543 3682 4299
Size: 416 53 83 399 24 2126 139 617
Cuts at: 4299 5257 442
Size: 958 1753
Fragments arranged by size:
2126 1753 958 617 416 399 139 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5228 1239
Size: 3989 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2652 4693 5558 5856 2652
Size: 2041 865 298 3364
Fragments arranged by size:
3364 2041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3280 5231 882
Size: 617 1781 1951 2219
Fragments arranged by size:
2219 1951 1781 617
BsaAI yAC'GTr
Cuts at: 493 1204 4359 493
Size: 711 3155 2702
Fragments arranged by size:
3155 2702 711
BsaBI GATnn'nnATC
Cuts at: 2171 2679 6562 2171
Size: 508 3883 2177
Fragments arranged by size:
3883 2177 508
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2191 2779 4375 4757
Size: 53 83 186 936 658 588 1596 382
Cuts at: 4757 5392 275
Size: 635 1451
Fragments arranged by size:
1596 1451 936 658 635 588 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5001 5399 5673 5820 6231 5001
Size: 398 274 147 411 5338
Fragments arranged by size:
5338 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6343
Fragments arranged by size:
6343 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6568
BseYI C'CCAG_C
Cuts at: 5771 6267 5771
Size: 496 6072
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 3099 1163
Size: 573 726 637 4632
Fragments arranged by size:
4632 726 637 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4018 4779 5807 1540
Size: 275 186 2017 761 1028 2301
Fragments arranged by size:
2301 2017 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2968 4629 4714 5785 2968
Size: 1661 85 1071 3751
Fragments arranged by size:
3751 1661 1071 85
BsmI GAATG_Cn'
Cuts at: 4079 4172 4079
Size: 93 6475
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2968 3044 3549 3887 4629 4714 5785
Size: 399 1178 76 505 338 742 85 1071
Cuts at: 5785 1391
Size: 2174
Fragments arranged by size:
2174 1178 1071 742 505 399 338 85
76
BspEI T'CCGG_A
Cuts at: 6231 6231
Size: 6568
BspHI T'CATG_A
Cuts at: 4457 6187 4457
Size: 1730 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3923 844
Size: 3079 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2145 4455 1394
Size: 429 322 2310 3507
Fragments arranged by size:
3507 2310 429 322
BsrDI GCAATG_nn'
Cuts at: 65 1248 5063 5237 65
Size: 1183 3815 174 1396
Fragments arranged by size:
3815 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5212 5399 1700
Size: 110 3402 187 2869
Fragments arranged by size:
3402 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3089 3326 96
Size: 2993 237 3338
Fragments arranged by size:
3338 2993 237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6568
BssSI C'ACGA_G
Cuts at: 2494 4628 5640 2494
Size: 2134 1012 3422
Fragments arranged by size:
3422 2134 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6568
BstBI TT'CG_AA
Cuts at: 2016 5372 2016
Size: 3356 3212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6568
BstXI CCAn_nnnn'nTGG
Cuts at: 3266 3266
Size: 6568
BstYI r'GATC_y
Cuts at: 1972 2992 3898 4265 4650 4667 6119 6205 6217
Size: 1020 906 367 385 17 1452 86 12
Cuts at: 6217 6563 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1020 906 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 4786
Fragments arranged by size:
4786 939 552 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5829
Fragments arranged by size:
5829 683 56
BtsI GCAGTG_nn'
Cuts at: 3087 3107 3838 4084 4878 4898 3087
Size: 20 731 246 794 20 4757
Fragments arranged by size:
4757 794 731 246 20 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 3872 3872
Size: 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5575 809
Size: 507 4259 1802
Fragments arranged by size:
4259 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2292 4015 4904
Size: 54 1425 50 275 18 462 1723 889
Cuts at: 4904 6533 8
Size: 1629 43
Fragments arranged by size:
1723 1629 1425 889 462 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4015 1537
Size: 275 2203 4090
Fragments arranged by size:
4090 2203 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 2135 3438 3439 4498 1353
Size: 660 122 1303 1 1059 3423
Fragments arranged by size:
3423 1303 1059 660 122 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3570 5155 5529 5675 387
Size: 1064 138 435 1546 1585 374 146 1280
Fragments arranged by size:
1585 1546 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2799 4631 5228 6015 6283 1239
Size: 393 678 489 1832 597 787 268 1524
Fragments arranged by size:
1832 1524 787 678 597 489 393 268
EcoRI G'AATT_C
Cuts at: 3877 4255 3877
Size: 378 6190
EcoRV GAT'ATC
Cuts at: 2198 2198
Size: 6568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2999 2999
Size: 6568
FspI TGC'GCA
Cuts at: 5074 5074
Size: 6568
HaeII r_GCGC'y
Cuts at: 1678 2129 2773 5715 1678
Size: 451 644 2942 2531
Fragments arranged by size:
2942 2531 644 451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 3842 3874 5209
Size: 32 135 32 680 32 1558 32 1335
Cuts at: 5209 5241 5283 5315 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1558 1335 680 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3912 4158 669
Size: 988 281 1974 246 3079
Fragments arranged by size:
3079 1974 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6568
HpaI GTT'AAC
Cuts at: 4158 4158
Size: 6568
KpnI G_GTAC'C
Cuts at: 3893 3893
Size: 6568
MfeI C'AATT_G
Cuts at: 4167 4167
Size: 6568
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5682 5866 1305
Size: 4377 184 2007
Fragments arranged by size:
4377 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4746
Fragments arranged by size:
4746 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2436 3479 4526 4885 5044 518
Size: 648 1270 1043 1047 359 159 2042
Fragments arranged by size:
2042 1270 1047 1043 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2819 3984 4661 5809 6054 1606
Size: 1213 1165 677 1148 245 2120
Fragments arranged by size:
2120 1213 1165 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5077
Fragments arranged by size:
5077 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6568
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NsiI A_TGCA'T
Cuts at: 2582 3476 2582
Size: 894 5674
NspI r_CATG'y
Cuts at: 1132 1229 3637 3926 5471 1132
Size: 97 2408 289 1545 2229
Fragments arranged by size:
2408 2229 1545 289 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6568
PciI A'CATG_T
Cuts at: 5467 5467
Size: 6568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2844 1108
Size: 1736 4832
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 6150 6182 4637
Size: 32 1481 32 5023
Fragments arranged by size:
5023 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3818 1613
Size: 2205 4363
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3349 3381 3349
Size: 32 6536
PstI C_TGCA'G
Cuts at: 830 2153 2992 3102 3920 830
Size: 1323 839 110 818 3478
Fragments arranged by size:
3478 1323 839 818 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 2819 2819
Size: 6568
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SapI GCTCTTCn'nnn_
Cuts at: 2135 3439 2135
Size: 1304 5264
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 3692 3781 4816 6528 3692
Size: 89 1035 1712 3732
Fragments arranged by size:
3732 1712 1035 89
SfcI C'TryA_G
Cuts at: 826 1045 2149 2277 2988 3098 3375 3916 5051
Size: 219 1104 128 711 110 277 541 1135
Cuts at: 5051 5732 5923 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1104 711 681 541 277 219
191 128 110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3895 1872
Size: 2023 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2667 4672 5573 5835 820
Size: 197 926 724 2005 901 262 1553
Fragments arranged by size:
2005 1553 926 901 724 262 197
SnaBI TAC'GTA
Cuts at: 493 4359 493
Size: 3866 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 3926 1132
Size: 2794 3774
SspI AAT'ATT
Cuts at: 5 52 2791 3198 4492 5
Size: 47 2739 407 1294 2081
Fragments arranged by size:
2739 2081 1294 407 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6568
StyI C'CwwG_G
Cuts at: 513 1065 2004 4007 513
Size: 552 939 2003 3074
Fragments arranged by size:
3074 2003 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3304 4595 4612 4765 4950 5395 325
Size: 1255 1724 1291 17 153 185 445 1498
Fragments arranged by size:
1724 1498 1291 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3089 3326 3690
Size: 274 80 33 51 1443 1112 237 364
Cuts at: 3690 3779 4814 6526 96
Size: 89 1035 1712 138
Fragments arranged by size:
1712 1443 1112 1035 364 274 237 138
89 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2080 2112 2330 2362 6528 6560 2080
Size: 32 218 32 4166 32 2088
Fragments arranged by size:
4166 2088 218 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6568
XbaI T'CTAG_A
Cuts at: 3904 3904
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6568
XmaI C'CCGG_G
Cuts at: 1870 3893 1870
Size: 2023 4545
XmnI GAAnn'nnTTC
Cuts at: 4697 4697
Size: 6568
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI