Restriction map for
pFN21AB6116
(Circular) (Six-base) MAPSORT of: pFN21AB6116.seq Check: 8434 from: 1 to: 6451
pFN21AB6116 6451 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2464 2464
Size: 6451
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4261 278
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3696 3794 1937
Size: 1759 98 4594
Fragments arranged by size:
4594 1759 98
Acc65I G'GTAC_C
Cuts at: 2148 3772 2148
Size: 1624 4827
AclI AA'CG_TT
Cuts at: 1563 4578 4951 1563
Size: 3015 373 3063
Fragments arranged by size:
3063 3015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3731 4514 5898 6166 1632
Size: 2099 783 1384 268 1917
Fragments arranged by size:
2099 1917 1384 783 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6254
AflIII A'CryG_T
Cuts at: 1203 3651 5350 6327 1203
Size: 2448 1699 977 1327
Fragments arranged by size:
2448 1699 1327 977
AgeI A'CCGG_T
Cuts at: 5282 5282
Size: 6451
AhdI GACnn_n'nnGTC
Cuts at: 5180 5180
Size: 6451
AleI CACnn'nnGTG
Cuts at: 1166 2267 1166
Size: 1101 5350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2504 3443 1184
Size: 1320 939 4192
Fragments arranged by size:
4192 1320 939
AlwNI CAG_nnn'CTG
Cuts at: 2062 2166 2656 2992 4194 5766 2062
Size: 104 490 336 1202 1572 2747
Fragments arranged by size:
2747 1572 1202 490 336 104
ApaI G_GGCC'C
Cuts at: 2021 2123 3277 2021
Size: 102 1154 5195
Fragments arranged by size:
5195 1154 102
ApaLI G'TGCA_C
Cuts at: 2786 4508 5664 2786
Size: 1722 1156 3573
Fragments arranged by size:
3573 1722 1156
ApoI r'AATT_y
Cuts at: 1493 3760 3986 4138 6247 1493
Size: 2267 226 152 2109 1697
Fragments arranged by size:
2267 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5005 160
Size: 872 3973 1606
Fragments arranged by size:
3973 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 2975 3293 3405 3456 3776 1870
Size: 73 229 803 318 112 51 320 4545
Fragments arranged by size:
4545 803 320 318 229 112 73 51
BamHI G'GATC_C
Cuts at: 3781 4148 3781
Size: 367 6084
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2148 2292 2552 2567 2903
Size: 325 217 627 361 144 260 15 336
Cuts at: 2903 2999 3242 3772 5227 618
Size: 96 243 530 1455 1842
Fragments arranged by size:
1842 1455 627 530 361 336 325 260
243 217 144 96 15
BanII G_rGCy'C
Cuts at: 1391 2021 2123 2403 2829 3277 3406 3705 3750
Size: 630 102 280 426 448 129 299 45
Cuts at: 3750 3770 1391
Size: 20 4072
Fragments arranged by size:
4072 630 448 426 299 280 129 102
45 20
BbeI G_GCGC'C
Cuts at: 2296 2296
Size: 6451
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2469 3531 928
Size: 971 570 1062 3848
Fragments arranged by size:
3848 1062 971 570
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2557 2591 2893 2927 4642 4676 1903
Size: 34 620 34 302 34 1715 34 3678
Fragments arranged by size:
3678 1715 620 302 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4343 5553 4343
Size: 1210 5241
BclI T'GATC_A
Cuts at: 1548 2634 1548
Size: 1086 5365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 2325 3336
Size: 107 122 71 631 605 439 214 1011
Cuts at: 3336 5062 136
Size: 1726 1525
Fragments arranged by size:
1726 1525 1011 631 605 439 214 122
107 71
BglII A'GATC_T
Cuts at: 6446 6446
Size: 6451
BlpI GC'TnA_GC
Cuts at: 3868 3868
Size: 6451
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2123 2790 3277 3375 4512 5668 1790
Size: 231 102 667 487 98 1137 1156 2573
Fragments arranged by size:
2573 1156 1137 667 487 231 102 98
BmgBI CAC'GTC
Cuts at: 1318 2770 2785 1318
Size: 1452 15 4984
Fragments arranged by size:
4984 1452 15
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4182 5140 442
Size: 416 53 83 399 24 2765 958 1753
Fragments arranged by size:
2765 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6451
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 6451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2694 2843 3087 5111 1239
Size: 1455 149 244 2024 2579
Fragments arranged by size:
2579 2024 1455 244 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3573 3689 4576 5441 5739 3573
Size: 116 887 865 298 4285
Fragments arranged by size:
4285 887 865 298 116
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3167 3179 5114 882
Size: 617 1668 12 1935 2219
Fragments arranged by size:
2219 1935 1668 617 12
BsaAI yAC'GTr
Cuts at: 493 1204 4242 493
Size: 711 3038 2702
Fragments arranged by size:
3038 2702 711
BsaBI GATnn'nnATC
Cuts at: 2693 6445 2693
Size: 3752 2699
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2293 2350 3610 4258
Size: 53 83 186 936 760 57 1260 648
Cuts at: 4258 4640 5275 275
Size: 382 635 1451
Fragments arranged by size:
1451 1260 936 760 648 635 382 186
83 57 53
BsaWI w'CCGG_w
Cuts at: 2410 3559 4884 5282 5556 5703 6114 2410
Size: 1149 1325 398 274 147 411 2747
Fragments arranged by size:
2747 1325 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 2873 2903 3437
Size: 30 165 30 1152 30 288 30 534
Cuts at: 3437 3467 1178
Size: 30 4162
Fragments arranged by size:
4162 1152 534 288 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 3578 3743 3758 1186
Size: 841 1551 165 15 3879
Fragments arranged by size:
3879 1551 841 165 15
BseYI C'CCAG_C
Cuts at: 2024 2095 2659 3246 5654 6150 2024
Size: 71 564 587 2408 496 2325
Fragments arranged by size:
2408 2325 587 564 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 2201 2492 1163
Size: 573 360 105 291 5122
Fragments arranged by size:
5122 573 360 291 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2115 3901 4662 5690 1540
Size: 275 186 114 1786 761 1028 2301
Fragments arranged by size:
2301 1786 1028 761 275 186 114
BsiHKAI G_wGCw'C
Cuts at: 2483 2790 2829 3705 3750 4512 4597 5668 2483
Size: 307 39 876 45 762 85 1071 3266
Fragments arranged by size:
3266 1071 876 762 307 85 45 39
BsmI GAATG_Cn'
Cuts at: 3962 4055 3962
Size: 93 6358
BsmBI CGTCTCn'nnnn_
Cuts at: 2810 2810
Size: 6451
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2123 2403 2483 2790 2829 3277
Size: 399 231 102 280 80 307 39 448
Cuts at: 3277 3375 3406 3705 3750 3770 4512 4597 5668
Size: 98 31 299 45 20 742 85 1071
Cuts at: 5668 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 448 399 307 299 280
231 102 98 85 80 45 39 31
20
BspEI T'CCGG_A
Cuts at: 3559 6114 3559
Size: 2555 3896
BspHI T'CATG_A
Cuts at: 4340 6070 4340
Size: 1730 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2464 3185 3806 844
Size: 1620 721 621 3489
Fragments arranged by size:
3489 1620 721 621
BsrBI CCG'CTC
Cuts at: 1394 1823 2344 3733 4338 1394
Size: 429 521 1389 605 3507
Fragments arranged by size:
3507 1389 605 521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2483 3187 4946 5120 65
Size: 1183 1235 704 1759 174 1396
Fragments arranged by size:
1759 1396 1235 1183 704 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5095 5282 1700
Size: 110 3285 187 2869
Fragments arranged by size:
3285 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6451
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6451
BssSI C'ACGA_G
Cuts at: 3740 4511 5523 3740
Size: 771 1012 4668
Fragments arranged by size:
4668 1012 771
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2062 794
Size: 1268 5183
BstBI TT'CG_AA
Cuts at: 5255 5255
Size: 6451
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6451
BstXI CCAn_nnnn'nTGG
Cuts at: 2281 2549 2885 2281
Size: 268 336 5847
Fragments arranged by size:
5847 336 268
BstYI r'GATC_y
Cuts at: 1972 3075 3781 4148 4533 4550 6002 6088 6100
Size: 1103 706 367 385 17 1452 86 12
Cuts at: 6100 6446 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1103 706 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2013 2082 3388 513
Size: 552 948 69 1306 3576
Fragments arranged by size:
3576 1306 948 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5712
Fragments arranged by size:
5712 683 56
BtsI GCAGTG_nn'
Cuts at: 2500 3427 3967 4761 4781 2500
Size: 927 540 794 20 4170
Fragments arranged by size:
4170 927 794 540 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3178 3213 529
Size: 35 2614 35 3767
Fragments arranged by size:
3767 2614 35 35
DraI TTT'AAA
Cuts at: 3755 3755
Size: 6451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2615 5458 809
Size: 507 1299 2843 1802
Fragments arranged by size:
2843 1802 1299 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2271 3164
Size: 54 1425 50 275 18 282 159 893
Cuts at: 3164 3898 4787 6416 8
Size: 734 889 1629 43
Fragments arranged by size:
1629 1425 893 889 734 282 275 159
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2112 3898 1537
Size: 275 300 1786 4090
Fragments arranged by size:
4090 1786 300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2915 4381 1353
Size: 1562 1466 3423
Fragments arranged by size:
3423 1562 1466
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5038 5412 5558 387
Size: 1064 138 3449 374 146 1280
Fragments arranged by size:
3449 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2827 3703 3748 2827
Size: 876 45 5530
Fragments arranged by size:
5530 876 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2694 2843 3087 3731 4514 5111 5898
Size: 393 1062 149 244 644 783 597 787
Cuts at: 5898 6166 1239
Size: 268 1524
Fragments arranged by size:
1524 1062 787 783 644 597 393 268
244 149
EcoNI CCTnn'n_nnAGG
Cuts at: 3539 3539
Size: 6451
EcoO109I rG'GnC_Cy
Cuts at: 2018 2119 2120 2163 3273 3274 3502 3553 3628
Size: 101 1 43 1110 1 228 51 75
Cuts at: 3628 2018
Size: 4841
Fragments arranged by size:
4841 1110 228 101 75 51 43 1
1
EcoRI G'AATT_C
Cuts at: 3760 4138 3760
Size: 378 6073
FspI TGC'GCA
Cuts at: 4957 4957
Size: 6451
HaeII r_GCGC'y
Cuts at: 1678 2296 5598 1678
Size: 618 3302 2531
Fragments arranged by size:
3302 2531 618
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 3060 3092 3437
Size: 32 135 32 983 32 473 32 345
Cuts at: 3437 3469 5092 5124 5166 5198 1373
Size: 32 1623 32 42 32 2626
Fragments arranged by size:
2626 1623 983 473 345 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 3697 3795 4041 669
Size: 988 281 400 1359 98 246 3079
Fragments arranged by size:
3079 1359 988 400 281 246 98
HindIII A'AGCT_T
Cuts at: 748 3194 748
Size: 2446 4005
HpaI GTT'AAC
Cuts at: 4041 4041
Size: 6451
KasI G'GCGC_C
Cuts at: 2292 2292
Size: 6451
KpnI G_GTAC'C
Cuts at: 2152 3776 2152
Size: 1624 4827
MfeI C'AATT_G
Cuts at: 4050 4050
Size: 6451
MluI A'CGCG_T
Cuts at: 6327 6327
Size: 6451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5565 5749 1305
Size: 4260 184 2007
Fragments arranged by size:
4260 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 10
Size: 54 1425 343 441 4188
Fragments arranged by size:
4188 1425 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 2279 2306 2327 2547 2883 3344
Size: 648 1101 12 27 21 220 336 461
Cuts at: 3344 4409 4768 4927 518
Size: 1065 359 159 2042
Fragments arranged by size:
2042 1101 1065 648 461 359 336 220
159 27 21 12
MspA1I CmG'CkG
Cuts at: 1606 2015 3250 3867 4544 5692 5937 1606
Size: 409 1235 617 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 677 617 409 245
NarI GG'CG_CC
Cuts at: 2293 2293
Size: 6451
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5899
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6451
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6451
NotI GC'GGCC_GC
Cuts at: 3898 3898
Size: 6451
NspI r_CATG'y
Cuts at: 1132 1229 2332 2668 3343 3655 3809 5354 1132
Size: 97 1103 336 675 312 154 1545 2229
Fragments arranged by size:
2229 1545 1103 675 336 312 154 97
PasI CC'CwG_GG
Cuts at: 1348 2160 1348
Size: 812 5639
PciI A'CATG_T
Cuts at: 3651 5350 3651
Size: 1699 4752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6451
PfoI T'CCnGG_A
Cuts at: 2517 2671 2517
Size: 154 6297
PmeI GTTT'AAAC
Cuts at: 3755 3755
Size: 6451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 6033 6065 4520
Size: 32 1481 32 4906
Fragments arranged by size:
4906 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3502 3553 3628 3502
Size: 51 75 6325
Fragments arranged by size:
6325 75 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6451
PsiI TTA'TAA
Cuts at: 4021 4021
Size: 6451
PspOMI G'GGCC_C
Cuts at: 2017 2119 3273 2017
Size: 102 1154 5195
Fragments arranged by size:
5195 1154 102
PstI C_TGCA'G
Cuts at: 830 2058 2997 3336 3803 830
Size: 1228 939 339 467 3478
Fragments arranged by size:
3478 1228 939 467 339
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6451
PvuII CAG'CTG
Cuts at: 3250 3250
Size: 6451
SacI G_AGCT'C
Cuts at: 2829 3705 3750 2829
Size: 876 45 5530
Fragments arranged by size:
5530 876 45
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 6451
SalI G'TCGA_C
Cuts at: 1936 3695 3793 1936
Size: 1759 98 4594
Fragments arranged by size:
4594 1759 98
SbfI CC_TGCA'GG
Cuts at: 3336 3803 3336
Size: 467 5984
ScaI AGT'ACT
Cuts at: 4699 6411 4699
Size: 1712 4739
SfcI C'TryA_G
Cuts at: 826 1045 2054 2587 2993 3332 3799 4934 5615
Size: 219 1009 533 406 339 467 1135 681
Cuts at: 5615 5806 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1009 681 533 467 406 339
219 191
SfoI GGC'GCC
Cuts at: 2294 2294
Size: 6451
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6451
SmaI CCC'GGG
Cuts at: 1872 2174 2977 3407 3458 3778 1872
Size: 302 803 430 51 320 4545
Fragments arranged by size:
4545 803 430 320 302 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3588 3704 4555 5456 5718 820
Size: 197 926 1645 116 851 901 262 1553
Fragments arranged by size:
1645 1553 926 901 851 262 197 116
SnaBI TAC'GTA
Cuts at: 493 4242 493
Size: 3749 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6451
SphI G_CATG'C
Cuts at: 1132 3809 1132
Size: 2677 3774
SspI AAT'ATT
Cuts at: 5 52 4375 5
Size: 47 4323 2081
Fragments arranged by size:
4323 2081 47
StuI AGG'CCT
Cuts at: 1517 2319 2753 3318 1517
Size: 802 434 565 4650
Fragments arranged by size:
4650 802 565 434
StyI C'CwwG_G
Cuts at: 513 1065 2265 3890 513
Size: 552 1200 1625 3074
Fragments arranged by size:
3074 1625 1200 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2273 2328 3260 4478 4495 4648 4833
Size: 1255 693 55 932 1218 17 153 185
Cuts at: 4833 5278 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1218 932 693 445 185 153
55 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4697 6409 96
Size: 274 80 33 51 1443 2720 1712 138
Fragments arranged by size:
2720 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6411 6443 6411
Size: 32 6419
Tth111I GACn'n_nGTC
Cuts at: 1316 2531 2804 3157 1316
Size: 1215 273 353 4610
Fragments arranged by size:
4610 1215 353 273
XbaI T'CTAG_A
Cuts at: 3787 3787
Size: 6451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2375 3395 2375
Size: 1020 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6451
XmaI C'CCGG_G
Cuts at: 1870 2172 2975 3405 3456 3776 1870
Size: 302 803 430 51 320 4545
Fragments arranged by size:
4545 803 430 320 302 51
XmnI GAAnn'nnTTC
Cuts at: 4580 4580
Size: 6451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4259 276
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI