Restriction map for
pFN21AB6110
(Circular) (Six-base) MAPSORT of: pFN21AB6110.seq Check: 3696 from: 1 to: 6589
pFN21AB6110 6589 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3038 3048 3565 3038
Size: 10 517 6062
Fragments arranged by size:
6062 517 10
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4399 278
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3932 1937
Size: 1995 4594
Acc65I G'GTAC_C
Cuts at: 3910 3910
Size: 6589
AclI AA'CG_TT
Cuts at: 1563 4716 5089 1563
Size: 3153 373 3063
Fragments arranged by size:
3153 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 3593 4652 6036 6304 1632
Size: 527 1434 1059 1384 268 1917
Fragments arranged by size:
1917 1434 1384 1059 527 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6392
AflIII A'CryG_T
Cuts at: 1203 3098 5488 6465 1203
Size: 1895 2390 977 1327
Fragments arranged by size:
2390 1895 1327 977
AgeI A'CCGG_T
Cuts at: 5420 5420
Size: 6589
AhdI GACnn_n'nnGTC
Cuts at: 3618 3861 5318 3618
Size: 243 1457 4889
Fragments arranged by size:
4889 1457 243
AleI CACnn'nnGTG
Cuts at: 1166 2777 2980 1166
Size: 1611 203 4775
Fragments arranged by size:
4775 1611 203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2862 1184
Size: 1678 4911
AlwNI CAG_nnn'CTG
Cuts at: 2762 4332 5904 2762
Size: 1570 1572 3447
Fragments arranged by size:
3447 1572 1570
ApaI G_GGCC'C
Cuts at: 2220 2330 3719 2220
Size: 110 1389 5090
Fragments arranged by size:
5090 1389 110
ApaLI G'TGCA_C
Cuts at: 2372 4646 5802 2372
Size: 2274 1156 3159
Fragments arranged by size:
3159 2274 1156
ApoI r'AATT_y
Cuts at: 1493 3898 4124 4276 6385 1493
Size: 2405 226 152 2109 1697
Fragments arranged by size:
2405 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5143 160
Size: 872 4111 1606
Fragments arranged by size:
4111 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
AvaI C'yCGr_G
Cuts at: 1870 1943 3914 1870
Size: 73 1971 4545
Fragments arranged by size:
4545 1971 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3533 3566 3533
Size: 33 6556
BamHI G'GATC_C
Cuts at: 3212 3919 4286 3212
Size: 707 367 5515
Fragments arranged by size:
5515 707 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 2315 3219 3763 3910
Size: 325 217 627 465 63 904 544 147
Cuts at: 3910 5365 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 904 627 544 465 325 217
147 63
BanII G_rGCy'C
Cuts at: 1391 2061 2115 2220 2330 2427 2709 3497 3719
Size: 670 54 105 110 97 282 788 222
Cuts at: 3719 3743 3908 1391
Size: 24 165 4072
Fragments arranged by size:
4072 788 670 282 222 165 110 105
97 54 24
BbeI G_GCGC'C
Cuts at: 3767 3767
Size: 6589
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2213 928
Size: 971 314 5304
Fragments arranged by size:
5304 971 314
BbvCI CC'TCA_GC
Cuts at: 3418 3418
Size: 6589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4780 4814 1903
Size: 34 2843 34 3678
Fragments arranged by size:
3678 2843 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2894 4481 5691 2894
Size: 1587 1210 3792
Fragments arranged by size:
3792 1587 1210
BclI T'GATC_A
Cuts at: 1548 2913 1548
Size: 1365 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2713 3431 3761
Size: 107 122 71 631 605 1041 718 330
Cuts at: 3761 5200 136
Size: 1439 1525
Fragments arranged by size:
1525 1439 1041 718 631 605 330 122
107 71
BglII A'GATC_T
Cuts at: 6584 6584
Size: 6589
BlpI GC'TnA_GC
Cuts at: 4006 4006
Size: 6589
Bme1580I G_kGCm'C
Cuts at: 1790 2155 2220 2330 2376 2666 2911 2987 3025
Size: 365 65 110 46 290 245 76 38
Cuts at: 3025 3224 3509 3719 4650 5806 1790
Size: 199 285 210 931 1156 2573
Fragments arranged by size:
2573 1156 931 365 290 285 245 210
199 110 76 65 46 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3293 3578 4320
Size: 416 53 83 399 24 1876 285 742
Cuts at: 4320 5278 442
Size: 958 1753
Fragments arranged by size:
1876 1753 958 742 416 399 285 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 3272 5249 1239
Size: 806 1227 1977 2579
Fragments arranged by size:
2579 1977 1227 806
Bpu10I CC'TnA_GC
Cuts at: 2579 3418 3488 2579
Size: 839 70 5680
Fragments arranged by size:
5680 839 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4714 5579 5877 3832
Size: 882 865 298 4544
Fragments arranged by size:
4544 882 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 5252 882
Size: 617 1092 2661 2219
Fragments arranged by size:
2661 2219 1092 617
BsaAI yAC'GTr
Cuts at: 493 1204 4380 493
Size: 711 3176 2702
Fragments arranged by size:
3176 2702 711
BsaBI GATnn'nnATC
Cuts at: 2171 6583 2171
Size: 4412 2177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3764 4396 4778 5413
Size: 53 83 186 936 2231 632 382 635
Cuts at: 5413 275
Size: 1451
Fragments arranged by size:
2231 1451 936 635 632 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2886 5022 5420 5694 5841 6252 2886
Size: 2136 398 274 147 411 3223
Fragments arranged by size:
3223 2136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6364
Fragments arranged by size:
6364 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3392 1186
Size: 2206 4383
BseYI C'CCAG_C
Cuts at: 2014 2323 2364 2502 2717 3733 5792 6288 2014
Size: 309 41 138 215 1016 2059 496 2315
Fragments arranged by size:
2315 2059 1016 496 309 215 138 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 2531 2594 1163
Size: 573 378 417 63 5158
Fragments arranged by size:
5158 573 417 378 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4039 4800 5828 1540
Size: 275 186 2038 761 1028 2301
Fragments arranged by size:
2301 2038 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2115 2376 2427 3743 4650 4735 5806 2061
Size: 54 261 51 1316 907 85 1071 2844
Fragments arranged by size:
2844 1316 1071 907 261 85 54 51
BsmI GAATG_Cn'
Cuts at: 4100 4193 4100
Size: 93 6496
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2115 2155 2220 2330 2376 2427
Size: 399 271 54 40 65 110 46 51
Cuts at: 2427 2666 2709 2911 2987 3025 3224 3497 3509
Size: 239 43 202 76 38 199 273 12
Cuts at: 3509 3719 3743 3908 4650 4735 5806 1391
Size: 210 24 165 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 273 271 239 210
202 199 165 110 85 76 65 54
51 46 43 40 38 24 12
BspEI T'CCGG_A
Cuts at: 2886 6252 2886
Size: 3366 3223
BspHI T'CATG_A
Cuts at: 4478 6208 4478
Size: 1730 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 3048 3565 3944 844
Size: 2194 10 517 379 3489
Fragments arranged by size:
3489 2194 517 379 10
BsrBI CCG'CTC
Cuts at: 1394 1823 4476 1394
Size: 429 2653 3507
Fragments arranged by size:
3507 2653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2396 5084 5258 65
Size: 1183 1148 2688 174 1396
Fragments arranged by size:
2688 1396 1183 1148 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5233 5420 1700
Size: 110 3423 187 2869
Fragments arranged by size:
3423 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6589
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6589
BssSI C'ACGA_G
Cuts at: 2108 4649 5661 2108
Size: 2541 1012 3036
Fragments arranged by size:
3036 2541 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6589
BstBI TT'CG_AA
Cuts at: 5393 5393
Size: 6589
BstEII G'GTnAC_C
Cuts at: 1184 2675 1184
Size: 1491 5098
BstYI r'GATC_y
Cuts at: 1972 3192 3212 3313 3382 3919 4286 4671 4688
Size: 1220 20 101 69 537 367 385 17
Cuts at: 4688 6140 6226 6238 6584 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1220 537 385 367 346 101
86 69 20 17 12
Bsu36I CC'TnA_GG
Cuts at: 3841 3841
Size: 6589
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5098
Fragments arranged by size:
5098 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5850
Fragments arranged by size:
5850 683 56
BtsI GCAGTG_nn'
Cuts at: 3745 4105 4899 4919 3745
Size: 360 794 20 5415
Fragments arranged by size:
5415 794 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2425 2460 529
Size: 35 1861 35 4658
Fragments arranged by size:
4658 1861 35 35
DraI TTT'AAA
Cuts at: 3893 3893
Size: 6589
DraIII CAC_nnn'GTG
Cuts at: 2035 2035
Size: 6589
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5596 809
Size: 507 4280 1802
Fragments arranged by size:
4280 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2118 2952
Size: 54 1425 50 275 18 253 35 834
Cuts at: 2952 3466 3673 3775 4036 4925 6554 8
Size: 514 207 102 261 889 1629 43
Fragments arranged by size:
1629 1425 889 834 514 275 261 253
207 102 54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 4036 1537
Size: 275 2224 4090
Fragments arranged by size:
4090 2224 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4519 1353
Size: 3166 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5176 5550 5696 387
Size: 1064 138 435 3152 374 146 1280
Fragments arranged by size:
3152 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2059 2113 2425 3741 2059
Size: 54 312 1316 4907
Fragments arranged by size:
4907 1316 312 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2159 3272 3593 4652 5249 6036
Size: 393 413 114 1113 321 1059 597 787
Cuts at: 6036 6304 1239
Size: 268 1524
Fragments arranged by size:
1524 1113 1059 787 597 413 393 321
268 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 6589
EcoO109I rG'GnC_Cy
Cuts at: 2216 2217 2327 3696 3807 3830 3845 2216
Size: 1 110 1369 111 23 15 4960
Fragments arranged by size:
4960 1369 111 110 23 15 1
EcoRI G'AATT_C
Cuts at: 3898 4276 3898
Size: 378 6211
FspI TGC'GCA
Cuts at: 5095 5095
Size: 6589
HaeII r_GCGC'y
Cuts at: 1678 3756 3767 5736 1678
Size: 2078 11 1969 2531
Fragments arranged by size:
2531 2078 1969 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3512 3544 3564 3596 3604
Size: 32 135 32 1940 32 20 32 8
Cuts at: 3604 3636 3688 3720 3847 3879 5230 5262 5304
Size: 32 52 32 127 32 1351 32 42
Cuts at: 5304 5336 1373
Size: 32 2626
Fragments arranged by size:
2626 1940 1351 135 127 52 42 32
32 32 32 32 32 32 32 32
20 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2844 3933 4179 669
Size: 988 281 906 1089 246 3079
Fragments arranged by size:
3079 1089 988 906 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6589
HpaI GTT'AAC
Cuts at: 4179 4179
Size: 6589
KasI G'GCGC_C
Cuts at: 3763 3763
Size: 6589
KpnI G_GTAC'C
Cuts at: 3914 3914
Size: 6589
MfeI C'AATT_G
Cuts at: 2785 4188 2785
Size: 1403 5186
MluI A'CGCG_T
Cuts at: 6465 6465
Size: 6589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5703 5887 1305
Size: 4398 184 2007
Fragments arranged by size:
4398 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 2954 3468 3675 10
Size: 54 1425 343 288 834 514 207 2924
Fragments arranged by size:
2924 1425 834 514 343 288 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2379 2570 2777 2980 3097 4547
Size: 648 870 343 191 207 203 117 1450
Cuts at: 4547 4906 5065 518
Size: 359 159 2042
Fragments arranged by size:
2042 1450 870 648 359 343 207 203
191 159 117
MspA1I CmG'CkG
Cuts at: 1606 2148 2464 2604 3422 4005 4682 5830 6075
Size: 542 316 140 818 583 677 1148 245
Cuts at: 6075 1606
Size: 2120
Fragments arranged by size:
2120 1148 818 677 583 542 316 245
140
NarI GG'CG_CC
Cuts at: 3764 3764
Size: 6589
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5098
Fragments arranged by size:
5098 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6589
NotI GC'GGCC_GC
Cuts at: 4036 4036
Size: 6589
NspI r_CATG'y
Cuts at: 1132 1229 2350 3102 3947 5492 1132
Size: 97 1121 752 845 1545 2229
Fragments arranged by size:
2229 1545 1121 845 752 97
PasI CC'CwG_GG
Cuts at: 1348 2212 3800 1348
Size: 864 1588 4137
Fragments arranged by size:
4137 1588 864
PciI A'CATG_T
Cuts at: 3098 5488 3098
Size: 2390 4199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3202 3272 1108
Size: 2094 70 4425
Fragments arranged by size:
4425 2094 70
PmeI GTTT'AAAC
Cuts at: 3893 3893
Size: 6589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 6171 6203 4658
Size: 32 1481 32 5044
Fragments arranged by size:
5044 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3696 3830 3845 3696
Size: 134 15 6440
Fragments arranged by size:
6440 134 15
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6589
PsiI TTA'TAA
Cuts at: 4159 4159
Size: 6589
PspOMI G'GGCC_C
Cuts at: 2216 2326 3715 2216
Size: 110 1389 5090
Fragments arranged by size:
5090 1389 110
PstI C_TGCA'G
Cuts at: 830 2851 3045 3752 3941 830
Size: 2021 194 707 189 3478
Fragments arranged by size:
3478 2021 707 194 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6589
PvuII CAG'CTG
Cuts at: 2148 2604 3422 2148
Size: 456 818 5315
Fragments arranged by size:
5315 818 456
SacI G_AGCT'C
Cuts at: 2061 2115 2427 3743 2061
Size: 54 312 1316 4907
Fragments arranged by size:
4907 1316 312 54
SalI G'TCGA_C
Cuts at: 1936 3931 1936
Size: 1995 4594
SanDI GG'GwC_CC
Cuts at: 3696 3696
Size: 6589
SbfI CC_TGCA'GG
Cuts at: 3941 3941
Size: 6589
ScaI AGT'ACT
Cuts at: 4837 6549 4837
Size: 1712 4877
SexAI A'CCwGG_T
Cuts at: 2829 2829
Size: 6589
SfcI C'TryA_G
Cuts at: 826 1045 2262 2847 3041 3068 3748 3937 5072
Size: 219 1217 585 194 27 680 189 1135
Cuts at: 5072 5753 5944 826
Size: 681 191 1471
Fragments arranged by size:
1471 1217 1135 681 680 585 219 194
191 189 27
SfoI GGC'GCC
Cuts at: 3765 3765
Size: 6589
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6589
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6589
SmaI CCC'GGG
Cuts at: 1872 3916 1872
Size: 2044 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4693 5594 5856 820
Size: 197 926 1868 882 901 262 1553
Fragments arranged by size:
1868 1553 926 901 882 262 197
SnaBI TAC'GTA
Cuts at: 493 4380 493
Size: 3887 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6589
SphI G_CATG'C
Cuts at: 1132 3947 1132
Size: 2815 3774
SspI AAT'ATT
Cuts at: 5 52 4513 5
Size: 47 4461 2081
Fragments arranged by size:
4461 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6589
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 3583 3670 4028 513
Size: 552 939 117 1462 87 358 3074
Fragments arranged by size:
3074 1462 939 552 358 117 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3855 4616 4633 4786 4971 5416
Size: 1255 1923 352 761 17 153 185 445
Cuts at: 5416 325
Size: 1498
Fragments arranged by size:
1923 1498 1255 761 445 352 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3291 4835 6547
Size: 274 80 33 51 1443 1314 1544 1712
Cuts at: 6547 96
Size: 138
Fragments arranged by size:
1712 1544 1443 1314 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2080 2112 3461 3493 3546 3578 6549 6581 2080
Size: 32 1349 32 53 32 2971 32 2088
Fragments arranged by size:
2971 2088 1349 53 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6589
XbaI T'CTAG_A
Cuts at: 3925 3925
Size: 6589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2069 2338 2776 2011
Size: 58 269 438 5824
Fragments arranged by size:
5824 438 269 58
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6589
XmaI C'CCGG_G
Cuts at: 1870 3914 1870
Size: 2044 4545
XmnI GAAnn'nnTTC
Cuts at: 4718 4718
Size: 6589
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4397 276
Size: 53 83 186 936 2863 2468
Fragments arranged by size:
2863 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI
BstXI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SapI SfiI SrfI SwaI