Restriction map for
pFN21AB6108
(Circular) (Six-base) MAPSORT of: pFN21AB6108.seq Check: 195 from: 1 to: 6556
pFN21AB6108 6556 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3139 3673 3139
Size: 534 6022
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2481 2638 3899 1937
Size: 544 157 1261 4594
Fragments arranged by size:
4594 1261 544 157
Acc65I G'GTAC_C
Cuts at: 3877 3877
Size: 6556
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3065 3356 3383 3805 4619 6003 6271 1632
Size: 1433 291 27 422 814 1384 268 1917
Fragments arranged by size:
1917 1433 1384 814 422 291 268 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 2720 5455 6432 1203
Size: 1517 2735 977 1327
Fragments arranged by size:
2735 1517 1327 977
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 3182 1166
Size: 2016 4540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3417 1184
Size: 2233 4323
AlwNI CAG_nnn'CTG
Cuts at: 2996 3067 3131 3397 4299 5871 2996
Size: 71 64 266 902 1572 3681
Fragments arranged by size:
3681 1572 902 266 71 64
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 2337 2439 2981 3865 4091 4243 6352 1493
Size: 844 102 542 884 226 152 2109 1697
Fragments arranged by size:
2109 1697 884 844 542 226 152 102
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 3881 1870
Size: 73 1938 4545
Fragments arranged by size:
4545 1938 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3749 3782 3749
Size: 33 6523
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3877 5332 618
Size: 325 217 627 2090 1455 1842
Fragments arranged by size:
2090 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3166 3455 3704 3711 3875 1391
Size: 1775 289 249 7 164 4072
Fragments arranged by size:
4072 1775 289 249 164 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2851 928
Size: 971 952 4633
Fragments arranged by size:
4633 971 952
BbvCI CC'TCA_GC
Cuts at: 2643 3540 2643
Size: 897 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4747 4781 1903
Size: 34 2810 34 3678
Fragments arranged by size:
3678 2810 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 3717 1548
Size: 2169 4387
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2018 5167 136
Size: 107 122 71 631 605 346 3149 1525
Fragments arranged by size:
3149 1525 631 605 346 122 107 71
BglII A'GATC_T
Cuts at: 6551 6551
Size: 6556
BlpI GC'TnA_GC
Cuts at: 2237 3139 3207 3973 2237
Size: 902 68 766 4820
Fragments arranged by size:
4820 902 766 68
Bme1580I G_kGCm'C
Cuts at: 1790 4617 5773 1790
Size: 2827 1156 2573
Fragments arranged by size:
2827 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2104 1318
Size: 786 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2441 2610 4287
Size: 416 53 83 399 24 1024 169 1677
Cuts at: 4287 5245 442
Size: 958 1753
Fragments arranged by size:
1753 1677 1024 958 416 399 169 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2451 3174 3357 3728 3734 5216 1239
Size: 1034 178 723 183 371 6 1482 2579
Fragments arranged by size:
2579 1482 1034 723 371 183 178 6
Bpu10I CC'TnA_GC
Cuts at: 2643 3540 2643
Size: 897 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3336 3816 4681 5546 5844 3336
Size: 480 865 865 298 4048
Fragments arranged by size:
4048 865 865 480 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3015 5219 882
Size: 617 1516 2204 2219
Fragments arranged by size:
2219 2204 1516 617
BsaAI yAC'GTr
Cuts at: 493 1204 4347 493
Size: 711 3143 2702
Fragments arranged by size:
3143 2702 711
BsaBI GATnn'nnATC
Cuts at: 6550 6550
Size: 6556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4363 4745 5380 275
Size: 53 83 186 936 2830 382 635 1451
Fragments arranged by size:
2830 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4989 5387 5661 5808 6219 4989
Size: 398 274 147 411 5326
Fragments arranged by size:
5326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3288 3318 3547 3577 1178
Size: 30 165 30 1885 30 229 30 4157
Fragments arranged by size:
4157 1885 229 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3003 3507 3674 1186
Size: 1817 504 167 4068
Fragments arranged by size:
4068 1817 504 167
BseYI C'CCAG_C
Cuts at: 5759 6255 5759
Size: 496 6060
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5983
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3166 3444 3455 4617 4702 5773 3166
Size: 278 11 1162 85 1071 3949
Fragments arranged by size:
3949 1162 1071 278 85 11
BsmI GAATG_Cn'
Cuts at: 2366 2499 4067 4160 2366
Size: 133 1568 93 4762
Fragments arranged by size:
4762 1568 133 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3573 3573
Size: 6556
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3166 3444 3455 3704 3711 3875 4617
Size: 399 1376 278 11 249 7 164 742
Cuts at: 4617 4702 5773 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1376 1071 742 399 278 249 164
85 11 7
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 4445 6175 4445
Size: 1730 4826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3139 3673 3911 844
Size: 2295 534 238 3489
Fragments arranged by size:
3489 2295 534 238
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 4443 1394
Size: 429 1810 810 3507
Fragments arranged by size:
3507 1810 810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5051 5225 65
Size: 1183 3803 174 1396
Fragments arranged by size:
3803 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2943 3411 5200 5387 1700
Size: 110 1133 468 1789 187 2869
Fragments arranged by size:
2869 1789 1133 468 187 110
BsrGI T'GTAC_A
Cuts at: 96 2124 3040 3379 96
Size: 2028 916 339 3273
Fragments arranged by size:
3273 2028 916 339
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 3033 4616 5628 3033
Size: 1583 1012 3961
Fragments arranged by size:
3961 1583 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3397 794
Size: 2603 3953
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6556
BstXI CCAn_nnnn'nTGG
Cuts at: 2116 2116
Size: 6556
BstYI r'GATC_y
Cuts at: 1972 3886 4253 4638 4655 6107 6193 6205 6551
Size: 1914 367 385 17 1452 86 12 346
Cuts at: 6551 1972
Size: 1977
Fragments arranged by size:
1977 1914 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2639 2639
Size: 6556
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 3585 513
Size: 552 939 9 1572 3484
Fragments arranged by size:
3484 1572 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5817
Fragments arranged by size:
5817 683 56
BtsI GCAGTG_nn'
Cuts at: 3758 4072 4866 4886 3758
Size: 314 794 20 5428
Fragments arranged by size:
5428 794 314 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DraIII CAC_nnn'GTG
Cuts at: 2526 2526
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2134 3413
Size: 54 1425 50 275 18 276 28 1279
Cuts at: 3413 3423 4003 4892 6521 8
Size: 10 580 889 1629 43
Fragments arranged by size:
1629 1425 1279 889 580 276 275 54
50 43 28 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3268 3824 4486 1353
Size: 1915 556 662 3423
Fragments arranged by size:
3423 1915 662 556
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5143 5517 5663 387
Size: 1064 138 3554 374 146 1280
Fragments arranged by size:
3554 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3164 3453 3164
Size: 289 6267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2451 3065 3174 3356 3357 3383
Size: 393 641 178 614 109 182 1 26
Cuts at: 3383 3728 3734 3805 4619 5216 6003 6271 1239
Size: 345 6 71 814 597 787 268 1524
Fragments arranged by size:
1524 814 787 641 614 597 393 345
268 182 178 109 71 26 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3648 3648
Size: 6556
EcoO109I rG'GnC_Cy
Cuts at: 2293 2993 2293
Size: 700 5856
EcoRI G'AATT_C
Cuts at: 3865 4243 3865
Size: 378 6178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2217 2810 2217
Size: 593 5963
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 2074 5703 1678
Size: 396 3629 2531
Fragments arranged by size:
3629 2531 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2313 2345 3288 3320 3334
Size: 32 135 32 741 32 943 32 14
Cuts at: 3334 3366 5197 5229 5271 5303 1373
Size: 32 1831 32 42 32 2626
Fragments arranged by size:
2626 1831 943 741 135 42 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3900 4146 669
Size: 988 281 1962 246 3079
Fragments arranged by size:
3079 1962 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2792 3368 748
Size: 2044 576 3936
Fragments arranged by size:
3936 2044 576
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 3881 3881
Size: 6556
MfeI C'AATT_G
Cuts at: 4155 4155
Size: 6556
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2308 5670 5854 1305
Size: 1003 3362 184 2007
Fragments arranged by size:
3362 2007 1003 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 10
Size: 54 1425 343 276 4458
Fragments arranged by size:
4458 1425 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 3182 4514 4873 5032 518
Size: 648 1059 957 1332 359 159 2042
Fragments arranged by size:
2042 1332 1059 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3143 3397 3972 4649 5797 6042 1606
Size: 1537 254 575 677 1148 245 2120
Fragments arranged by size:
2120 1537 1148 677 575 254 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3585 513
Size: 552 939 1581 3484
Fragments arranged by size:
3484 1581 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 2724 3404 3914 5459 1132
Size: 97 1495 680 510 1545 2229
Fragments arranged by size:
2229 1545 1495 680 510 97
PasI CC'CwG_GG
Cuts at: 1348 3092 1348
Size: 1744 4812
PciI A'CATG_T
Cuts at: 2720 5455 2720
Size: 2735 3821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2151 2213 1108
Size: 1043 62 5451
Fragments arranged by size:
5451 1043 62
PfoI T'CCnGG_A
Cuts at: 2289 3190 3479 3750 2289
Size: 901 289 271 5095
Fragments arranged by size:
5095 901 289 271
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4625 4657 6138 6170 4625
Size: 32 1481 32 5011
Fragments arranged by size:
5011 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2993 2293
Size: 700 5856
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6556
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2521 2553 2521
Size: 32 6524
PstI C_TGCA'G
Cuts at: 830 2070 3908 830
Size: 1240 1838 3478
Fragments arranged by size:
3478 1838 1240
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
PvuII CAG'CTG
Cuts at: 3143 3397 3143
Size: 254 6302
SacI G_AGCT'C
Cuts at: 3166 3455 3166
Size: 289 6267
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 4804 6516 4804
Size: 1712 4844
SfcI C'TryA_G
Cuts at: 826 1045 2066 2308 3555 3904 5039 5720 5911
Size: 219 1021 242 1247 349 1135 681 191
Cuts at: 5911 826
Size: 1471
Fragments arranged by size:
1471 1247 1135 1021 681 349 242 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3351 3831 4660 5561 5823 820
Size: 197 926 1408 480 829 901 262 1553
Fragments arranged by size:
1553 1408 926 901 829 480 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3404 3914 1132
Size: 2272 510 3774
Fragments arranged by size:
3774 2272 510
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 3221 1517
Size: 1704 4852
StyI C'CwwG_G
Cuts at: 513 1065 2004 3420 3471 3585 3995 513
Size: 552 939 1416 51 114 410 3074
Fragments arranged by size:
3074 1416 939 552 410 114 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2934 3511 4583 4600 4753 4938 5383
Size: 1255 1354 577 1072 17 153 185 445
Cuts at: 5383 325
Size: 1498
Fragments arranged by size:
1498 1354 1255 1072 577 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 3040 3119
Size: 274 80 33 51 1443 147 916 79
Cuts at: 3119 3379 4802 6514 96
Size: 260 1423 1712 138
Fragments arranged by size:
1712 1443 1423 916 274 260 147 138
80 79 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6516 6548 6516
Size: 32 6524
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6556
XbaI T'CTAG_A
Cuts at: 3892 3892
Size: 6556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2426 2011
Size: 415 6141
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 3068 4685 3068
Size: 1617 4939
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI Bsu36I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI